##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename SV23_ACAGTG_L008_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11074770 Filtered Sequences 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.441087805886713 31.0 30.0 31.0 25.0 34.0 2 30.323292583051387 31.0 30.0 31.0 27.0 34.0 3 30.694808650653695 31.0 30.0 31.0 27.0 34.0 4 32.51320027413662 35.0 32.0 37.0 25.0 37.0 5 33.163655136856114 35.0 33.0 37.0 28.0 37.0 6 33.38975554345598 35.0 33.0 37.0 28.0 37.0 7 33.38632414036589 35.0 33.0 37.0 28.0 37.0 8 33.61032599322604 35.0 33.0 37.0 28.0 37.0 9 35.06255335325248 37.0 34.0 39.0 29.0 39.0 10 34.959190484317055 37.0 34.0 39.0 28.0 39.0 11 34.95010686452179 37.0 34.0 39.0 28.0 39.0 12 35.11686202061081 37.0 34.0 39.0 29.0 39.0 13 34.88827957600925 37.0 34.0 39.0 27.0 39.0 14 35.72449170501961 38.0 34.0 40.0 27.0 41.0 15 36.10518286158539 38.0 34.0 40.0 30.0 41.0 16 35.7556174981512 38.0 34.0 40.0 27.0 41.0 17 35.00388269914409 38.0 33.0 40.0 27.0 41.0 18 35.4005848428455 38.0 34.0 40.0 27.0 41.0 19 35.36287182487763 38.0 34.0 40.0 27.0 41.0 20 35.5350197791918 38.0 34.0 40.0 27.0 41.0 21 35.09621147888399 38.0 33.0 40.0 27.0 41.0 22 35.17726742857865 38.0 33.0 40.0 27.0 41.0 23 35.35093685918534 38.0 34.0 40.0 27.0 41.0 24 35.64481384263511 38.0 34.0 40.0 27.0 41.0 25 35.71370105203088 38.0 34.0 40.0 27.0 41.0 26 35.18651981034369 38.0 34.0 40.0 27.0 41.0 27 34.61215772426877 37.0 33.0 40.0 25.0 40.0 28 34.13597754174579 37.0 32.0 40.0 24.0 40.0 29 33.99297430104643 37.0 32.0 39.0 24.0 40.0 30 34.321865555672936 37.0 32.0 39.0 25.0 40.0 31 34.38362503239345 37.0 33.0 39.0 25.0 40.0 32 34.386293891430704 37.0 33.0 39.0 25.0 40.0 33 34.16709800745298 37.0 32.0 39.0 24.0 40.0 34 34.841211149306034 37.0 33.0 40.0 25.0 40.0 35 34.314322735370574 37.0 33.0 40.0 24.0 40.0 36 34.874381318979985 38.0 33.0 40.0 25.0 40.0 37 34.5318663954195 37.0 33.0 40.0 24.0 40.0 38 34.586692003535966 37.0 33.0 40.0 25.0 40.0 39 33.974335539248216 37.0 32.0 40.0 23.0 40.0 40 33.59982256967865 37.0 31.0 40.0 21.0 40.0 41 33.977524589675454 37.0 32.0 40.0 24.0 40.0 42 33.96311164927127 37.0 32.0 40.0 24.0 40.0 43 33.80485355452077 37.0 31.0 40.0 23.0 40.0 44 33.75955229770009 37.0 31.0 40.0 23.0 40.0 45 33.376674188267565 37.0 31.0 40.0 20.0 40.0 46 32.96218810864695 37.0 30.0 39.0 18.0 40.0 47 33.562199937335045 37.0 31.0 40.0 23.0 40.0 48 32.92923148742592 36.0 30.0 39.0 18.0 40.0 49 33.00406554718518 36.0 30.0 39.0 19.0 40.0 50 33.43135252470255 37.0 31.0 39.0 22.0 40.0 51 32.496541869492546 36.0 30.0 39.0 19.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 7.0 10 23.0 11 40.0 12 64.0 13 83.0 14 215.0 15 546.0 16 1209.0 17 2747.0 18 5552.0 19 10191.0 20 17386.0 21 28074.0 22 43880.0 23 65896.0 24 96747.0 25 136148.0 26 189602.0 27 255019.0 28 337988.0 29 433610.0 30 545703.0 31 672399.0 32 805576.0 33 940962.0 34 1081292.0 35 1208766.0 36 1293951.0 37 1279647.0 38 1111113.0 39 510321.0 40 13.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.164234293574964 26.12697364160703 29.241149992291444 24.467642072526562 2 20.577510864785452 28.05432528169885 28.8712542111484 22.496909642367292 3 22.11274816542465 28.01433348051472 28.53085888013927 21.342059473921353 4 22.114003270496813 26.104876218648332 30.20402229572262 21.577098215132235 5 21.0840524836703 28.274285697774555 28.980070807861374 21.661591010693776 6 21.59797449518139 28.488320750679247 27.74044968879715 22.173255065342214 7 21.328551292713076 28.343613456532278 28.674058242293064 21.653777008461574 8 21.236540352531023 28.248180323383693 28.096484170777362 22.418795153307926 9 21.260297053573122 28.129297493311373 28.39943402887825 22.210971424237254 10 21.484301705588468 28.186689204380766 27.91750979930057 22.41149929073019 11 21.261958487625478 28.058117685514013 28.17932110553989 22.50060272132062 12 21.48089757168772 28.117839016069862 27.87682272408366 22.524440688158762 13 21.244703050266505 28.15019183242632 28.148304660051632 22.456800457255547 14 21.43865741681317 28.206951476193186 27.889455040601298 22.464936066392347 15 21.58833998358431 28.149993182702666 27.79076224607825 22.470904587634777 16 21.35426213530341 28.07021266567185 28.073815454501293 22.501709744523446 17 21.179678472607545 28.006779214072637 28.32695149620661 22.48659081711321 18 21.45928989947421 28.026550438519266 28.04192773303644 22.472231928970082 19 21.22249533578973 27.90012206135791 28.411447004079005 22.465935598773353 20 21.439614547299854 27.818455823461797 28.236559314550096 22.50537031468825 21 21.219114928027086 27.758215220556444 28.4802469541586 22.542422897257865 22 21.186786788522213 27.847823175880254 28.40571926336587 22.559670772231666 23 21.357826493727522 27.913223657281648 28.224759363701796 22.504190485289033 24 21.736622972756997 27.94417400993429 27.82483067368442 22.494372343624292 25 21.751168144023737 27.87693192876432 27.95335893722394 22.41854098998801 26 21.70280974227158 27.97103492655698 27.975355104136163 22.350800227035272 27 21.757751745441077 27.842228384078325 28.010766187667656 22.38925368281294 28 21.655084224283492 27.82851156130456 28.251629328601354 22.264774885810596 29 21.83692972033548 27.757749470002715 28.047064465654696 22.358256344007113 30 22.020574618303513 27.80281940980461 27.774118117108497 22.402487854783377 31 21.950124513092362 27.78498888325946 27.855311082274714 22.409575521373462 32 21.915731922143408 27.886235472424282 27.892321409839504 22.305711195592803 33 21.979830516234074 27.780541395588564 27.844950897349065 22.394677190828297 34 21.889219416326135 27.937769171883225 27.834712761863926 22.338298649926713 35 21.683104349526012 27.86049768593243 28.224915358537128 22.23148260600443 36 22.03842627876794 28.00607471271686 27.72071746706588 22.23478154144932 37 21.839081186521938 27.863388692843778 28.103174124309422 22.19435599632486 38 21.966327233359358 27.891825297959155 27.900657463514033 22.24119000516745 39 21.88005992559702 27.72136042594649 28.172906989410773 22.225672659045717 40 21.688203805426802 27.706119759790145 28.333861756971423 22.271814677811637 41 21.95329259974345 27.823998818192543 27.973512631921693 22.249195950142315 42 21.92891350680583 27.79554845270485 27.99752447949987 22.278013560989454 43 21.83043599885938 27.776658104780083 28.175415195515676 22.217490700844866 44 21.85317729546402 27.834923986089827 28.046869524567146 22.265029193879005 45 21.795193935707793 27.725017933008317 28.252368502863412 22.22741962842048 46 21.612938624715667 27.734842008622678 28.511786299902504 22.14043306675915 47 22.033632001320523 27.90232385648586 27.801496659907226 22.262547482286386 48 21.607538763612204 27.684760642196192 28.541950642350628 22.165749951840972 49 21.8373355659587 27.738768744196097 28.227283477888964 22.196612211956243 50 22.01081899161508 27.804862631495535 27.8906759002695 22.29364247661988 51 21.903044169196807 27.68233851606482 28.104089769877188 22.310527544861188 >>END_MODULE >>Per base GC content pass #Base %GC 1 44.63187636610152 2 43.074420507152745 3 43.45480763934601 4 43.69110148562905 5 42.745643494364074 6 43.77122956052361 7 42.98232830117465 8 43.65533550583895 9 43.471268477810376 10 43.895800996318656 11 43.762561208946096 12 44.00533825984648 13 43.701503507522055 14 43.90359348320552 15 44.05924457121909 16 43.85597187982685 17 43.66626928972075 18 43.93152182844429 19 43.68843093456308 20 43.944984861988104 21 43.761537825284954 22 43.746457560753875 23 43.86201697901655 24 44.23099531638129 25 44.16970913401174 26 44.053609969306855 27 44.14700542825402 28 43.91985911009409 29 44.19518606434259 30 44.423062473086894 31 44.359700034465824 32 44.221443117736214 33 44.37450770706237 34 44.22751806625285 35 43.914586955530446 36 44.27320782021726 37 44.033437182846804 38 44.20751723852681 39 44.10573258464274 40 43.960018483238436 41 44.202488549885764 42 44.206927067795284 43 44.04792669970424 44 44.118206489343024 45 44.02261356412828 46 43.75337169147482 47 44.29617948360691 48 43.77328871545318 49 44.033947777914946 50 44.304461468234955 51 44.213571714058 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 32.0 1 66.0 2 100.0 3 155.5 4 211.0 5 316.0 6 421.0 7 646.5 8 872.0 9 1324.5 10 1777.0 11 2715.5 12 3654.0 13 5715.5 14 7777.0 15 11180.0 16 14583.0 17 20644.0 18 26705.0 19 36280.0 20 45855.0 21 59918.0 22 73981.0 23 93816.5 24 113652.0 25 140778.5 26 203439.0 27 238973.0 28 284692.0 29 330411.0 30 385223.5 31 440036.0 32 499863.0 33 559690.0 34 613730.5 35 667771.0 36 702725.5 37 737680.0 38 758826.5 39 779973.0 40 783560.0 41 787147.0 42 789439.0 43 791731.0 44 788794.5 45 785858.0 46 774282.5 47 762707.0 48 740024.5 49 717342.0 50 682558.5 51 647775.0 52 600629.5 53 553484.0 54 500872.5 55 448261.0 56 399397.0 57 350533.0 58 306903.0 59 263273.0 60 227659.5 61 192046.0 62 165038.5 63 138031.0 64 119036.5 65 100042.0 66 87470.5 67 74899.0 68 65697.0 69 56495.0 70 50715.0 71 44935.0 72 40531.0 73 36127.0 74 32867.0 75 26495.5 76 23384.0 77 20698.0 78 18012.0 79 15826.0 80 13640.0 81 11769.5 82 9899.0 83 8355.5 84 6812.0 85 5642.0 86 4472.0 87 3607.5 88 2743.0 89 2200.0 90 1657.0 91 1314.0 92 971.0 93 731.0 94 491.0 95 357.0 96 223.0 97 161.0 98 99.0 99 57.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024027587028895406 2 0.0 3 0.0 4 0.0 5 9.93248618255729E-5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.444725262917424E-4 17 1.3828729625987717 18 0.0 19 1.0835439471880679E-4 20 0.0 21 0.0029436277231942518 22 0.0011467506774407053 23 0.00250118061142579 24 0.0 25 0.001363459466878319 26 0.09399743741856491 27 0.027720665982228075 28 0.041228847190505985 29 0.28703982114301246 30 0.018943960010004722 31 2.799155196902509E-4 32 0.14889699740942702 33 0.0028623619271551464 34 0.01104311872842506 35 0.0010383962827218984 36 0.017905563727282824 37 0.03270496813929319 38 0.014519488892320112 39 0.0334815079681113 40 0.008605144847251906 41 0.0018781428417926514 42 0.02852429440972589 43 9.751895524692613E-4 44 0.023350372061902866 45 0.02716083494284757 46 0.025327839765521092 47 0.0034041339007491807 48 0.01877239888503328 49 0.012722611846566563 50 0.0065464113475945775 51 0.008523879051212801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1.107477E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 4.272509312831891 #Duplication Level Relative count 1 100.0 2 1.1731100612169918 3 0.45175345542233514 4 0.3099660020595404 5 0.22924029868510248 6 0.1573116270886482 7 0.13506031141492494 8 0.11850119277401461 9 0.09004020760994996 10++ 0.8295083494181023 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content pass >>END_MODULE