##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV22_GCCAAT_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7769597	
Filtered Sequences	0	
Sequence length	51	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.401147575607848	31.0	30.0	31.0	25.0	34.0
2	30.260961926339295	31.0	30.0	31.0	27.0	34.0
3	30.618131802717695	31.0	30.0	31.0	27.0	34.0
4	32.4565077184827	35.0	32.0	37.0	25.0	37.0
5	33.061481695897484	35.0	33.0	37.0	28.0	37.0
6	33.210698959032236	35.0	33.0	37.0	28.0	37.0
7	33.18001422725014	35.0	33.0	37.0	28.0	37.0
8	33.41868979819674	35.0	33.0	37.0	28.0	37.0
9	34.82330241838798	37.0	34.0	39.0	28.0	39.0
10	34.69326735994158	37.0	33.0	39.0	27.0	39.0
11	34.6646783610527	37.0	33.0	39.0	27.0	39.0
12	34.8242013067087	37.0	34.0	39.0	27.0	39.0
13	34.58158486212348	37.0	33.0	39.0	27.0	39.0
14	35.356748490301364	38.0	33.0	40.0	27.0	41.0
15	35.7078111001124	38.0	34.0	40.0	27.0	41.0
16	35.303127305058425	38.0	33.0	40.0	27.0	41.0
17	34.517164275058285	37.0	33.0	40.0	25.0	41.0
18	34.84439077084693	37.0	33.0	40.0	26.0	40.0
19	34.741745035167206	37.0	32.0	40.0	25.0	40.0
20	34.83053033509975	37.0	33.0	40.0	26.0	40.0
21	34.277779014793175	37.0	32.0	40.0	25.0	40.0
22	34.33300864896854	37.0	32.0	39.0	25.0	40.0
23	34.456676324396234	37.0	32.0	39.0	25.0	40.0
24	34.68405967516719	37.0	32.0	39.0	26.0	40.0
25	34.682450711407554	37.0	33.0	39.0	25.0	40.0
26	34.00570917642189	37.0	32.0	39.0	24.0	40.0
27	33.28764606452561	37.0	31.0	39.0	19.0	40.0
28	32.72530904756064	36.0	30.0	39.0	18.0	40.0
29	32.43505139327046	36.0	30.0	39.0	17.0	40.0
30	32.66345783442822	36.0	30.0	39.0	18.0	40.0
31	32.65922415280998	36.0	30.0	39.0	18.0	40.0
32	32.556903144397324	36.0	30.0	39.0	18.0	40.0
33	32.24697728852603	36.0	30.0	39.0	16.0	40.0
34	32.95219739196254	36.0	30.0	39.0	20.0	40.0
35	32.416140759939026	36.0	30.0	39.0	16.0	40.0
36	32.883144904426835	36.0	30.0	39.0	19.0	40.0
37	32.48326341765216	36.0	30.0	39.0	16.0	40.0
38	32.429059447999684	36.0	30.0	39.0	16.0	40.0
39	31.709637578371183	35.0	28.0	39.0	15.0	40.0
40	31.219353075841642	35.0	27.0	39.0	13.0	40.0
41	31.506793853014514	35.0	28.0	39.0	15.0	40.0
42	31.38908324331365	35.0	28.0	39.0	14.0	40.0
43	31.15231085988115	35.0	27.0	39.0	13.0	40.0
44	31.03839362582126	35.0	27.0	39.0	13.0	40.0
45	30.554885665241066	34.0	26.0	38.0	11.0	40.0
46	30.03920962696006	34.0	24.0	38.0	9.0	40.0
47	30.51701446033816	34.0	26.0	38.0	12.0	40.0
48	29.836617008578436	34.0	24.0	38.0	9.0	40.0
49	29.783787885008707	33.0	24.0	38.0	10.0	40.0
50	30.115448845030187	33.0	25.0	38.0	11.0	40.0
51	29.208319427635693	33.0	24.0	38.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	10.0
11	28.0
12	98.0
13	277.0
14	878.0
15	2191.0
16	5261.0
17	10867.0
18	19924.0
19	32393.0
20	49084.0
21	68776.0
22	91279.0
23	118026.0
24	148583.0
25	183764.0
26	224284.0
27	273477.0
28	325503.0
29	386450.0
30	451022.0
31	516583.0
32	581171.0
33	643609.0
34	700054.0
35	737046.0
36	744399.0
37	686577.0
38	546201.0
39	221772.0
40	4.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.322400944293115	21.925103055165167	28.188897758694182	30.563598241847533
2	23.260871831576335	24.26535121448384	25.39467619749132	27.079100756448497
3	25.365266692725502	23.877969475122068	25.14954121816099	25.607222613991432
4	25.277565876325376	21.740149456915205	26.994257231102203	25.98802743565722
5	25.149464224923214	23.958100722239347	26.057929734985848	24.834505317851598
6	25.675321384107825	24.560720459503884	23.909991728013694	25.853966428374598
7	25.686467393353862	24.47866987180931	25.056421845302918	24.778440889533908
8	25.222247691868706	24.54521128959456	24.263549319224666	25.968991699312078
9	25.082690904045602	24.07885505515923	24.79588066150664	26.042573379288527
10	25.379810561603133	24.19085571619738	23.889141225728956	26.54019249647054
11	25.251142369417618	23.98090917714265	24.17153682488294	26.596411628556798
12	25.537746681069816	24.1007995652799	23.70673794277876	26.654715810871526
13	25.290076177696218	24.272545924840117	24.03474723335071	26.40263066411295
14	25.49722977909922	24.230484541218804	23.760138395852447	26.512147283829524
15	25.681718112277895	24.266277903474272	23.603077482654506	26.448926501593327
16	25.345324017866073	24.029707113132268	23.992330609078707	26.632638259922953
17	25.27590130717871	23.923889978461816	24.31278291945147	26.487425794908003
18	25.574170706665996	23.948449321116655	24.00595552124518	26.47142445097217
19	25.29241568731426	23.74773958471425	24.3273711997673	26.632473528204194
20	25.56145447440839	23.681253995541855	24.180790844106845	26.57650068594291
21	25.397639953972924	23.5775368306703	24.40541925054123	26.619403964815547
22	25.377188438160637	23.67720034365035	24.18439994722972	26.761211270959294
23	25.6036878981318	23.77788692374661	23.949894792553156	26.668530385568435
24	26.0661138537816	23.617531256769173	23.53755284862265	26.778802040826573
25	26.108687025893484	23.503249830136703	23.712890548830597	26.675172595139212
26	26.034642829952404	23.657867626778973	23.677976601706906	26.62951294156172
27	26.120978811572567	23.483658022061547	23.660908431521836	26.73445473484405
28	25.99341589728099	23.583206314846702	23.9237806675932	26.499597120279105
29	26.275050315183734	23.37421139539701	23.703352388253187	26.64738590116607
30	26.468983278393626	23.36601738392086	23.49127141596143	26.673727921724083
31	26.307946455174775	23.392004571684517	23.455251355770883	26.844797617369824
32	26.27832857264748	23.5470800081354	23.534474780910067	26.640116638307056
33	26.371437731928005	23.317437176156716	23.444577259223802	26.866547832691477
34	26.445453353037816	23.501713458374667	23.446878671340237	26.605954517247277
35	26.016003060165666	23.457335941475876	23.82705018615064	26.69961081220782
36	26.511633894377756	23.492189360538088	23.251324300839958	26.7448524442442
37	26.162146963066114	23.579985827538863	23.695832307649525	26.562034901745502
38	26.41138437520274	23.507956262183864	23.455603624496554	26.62505573811684
39	26.17120128213117	23.240211106687877	23.97505725455217	26.613530356628782
40	25.96652974515918	23.332253819317156	24.00570580651539	26.69551062900827
41	26.46573589197268	23.28863514265246	23.62510718272947	26.620521782645394
42	26.38967403849731	23.20685383145642	23.538931883831644	26.86454024621462
43	26.203131881865456	23.23781127107822	23.84576435540241	26.713292491653917
44	26.315568589925718	23.229369197791048	23.55079707496512	26.904265137318113
45	26.31535514370021	23.180380969055918	23.801682896559537	26.702580990684332
46	25.934173763046502	23.17009696443976	24.219494771484918	26.676234501028812
47	26.641665762469014	23.38206218588785	23.19272770238936	26.783544349253773
48	25.96460111600985	23.095562157104126	24.23832205381651	26.701514673069514
49	26.346780629714488	23.121283114074608	23.79078598460469	26.741150271606212
50	26.590097217568797	23.131947985015966	23.325549045838052	26.952405751577185
51	26.547827126776006	22.885716098802572	23.434467679945197	27.13198909447622
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.88599918614065
2	50.33997258802484
3	50.97248930671694
4	51.26559331198259
5	49.98396954277481
6	51.52928781248243
7	50.46490828288778
8	51.19123939118077
9	51.125264283334126
10	51.920003058073675
11	51.847553997974416
12	52.19246249194135
13	51.69270684180917
14	52.00937706292874
15	52.130644613871226
16	51.977962277789025
17	51.763327102086706
18	52.04559515763816
19	51.924889215518455
20	52.137955160351304
21	52.01704391878847
22	52.13839970911993
23	52.272218283700234
24	52.84491589460818
25	52.783859621032704
26	52.66415577151412
27	52.85543354641662
28	52.493013017560095
29	52.92243621634981
30	53.14271120011771
31	53.1527440725446
32	52.91844521095454
33	53.237985564619486
34	53.0514078702851
35	52.71561387237348
36	53.256486338621954
37	52.72418186481161
38	53.036440113319586
39	52.78473163875995
40	52.662040374167454
41	53.086257674618075
42	53.254214284711935
43	52.916424373519376
44	53.21983372724384
45	53.01793613438455
46	52.61040826407532
47	53.42521011172279
48	52.666115789079356
49	53.0879309013207
50	53.54250296914598
51	53.67981622125223
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	24.0
2	35.0
3	48.0
4	61.0
5	96.5
6	132.0
7	199.5
8	267.0
9	433.5
10	600.0
11	997.0
12	1394.0
13	2212.0
14	3030.0
15	4636.0
16	6242.0
17	8973.0
18	11704.0
19	15907.0
20	20110.0
21	26296.5
22	32483.0
23	41214.5
24	49946.0
25	61637.5
26	88397.5
27	103466.0
28	123193.0
29	142920.0
30	166455.5
31	189991.0
32	214637.0
33	239283.0
34	262282.5
35	285282.0
36	299471.0
37	313660.0
38	323816.5
39	333973.0
40	336739.0
41	339505.0
42	345099.5
43	350694.0
44	355404.5
45	360115.0
46	363241.5
47	366368.0
48	368338.0
49	370308.0
50	369574.5
51	368841.0
52	364395.0
53	359949.0
54	350268.5
55	340588.0
56	332803.5
57	325019.0
58	318179.5
59	311340.0
60	305913.0
61	300486.0
62	294833.0
63	289180.0
64	282614.5
65	276049.0
66	270080.5
67	264112.0
68	254633.0
69	245154.0
70	234686.0
71	224218.0
72	211680.5
73	199143.0
74	184840.5
75	155379.0
76	140220.0
77	125079.5
78	109939.0
79	96877.0
80	83815.0
81	71790.5
82	59766.0
83	50666.5
84	41567.0
85	34473.5
86	27380.0
87	22234.0
88	17088.0
89	13557.0
90	10026.0
91	7858.5
92	5691.0
93	4240.5
94	2790.0
95	2013.0
96	1236.0
97	840.5
98	445.0
99	275.5
100	106.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.021494036305872752
2	0.0
3	0.0
4	0.0
5	1.5444816507213953E-4
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.2870680422678293E-4
17	1.358294387726931
18	0.0
19	9.009476295874805E-5
20	0.0
21	0.003307764868628321
22	9.26688990432837E-4
23	0.0023810758781954845
24	0.0
25	0.0014672575681853254
26	0.08873047083394416
27	0.026449248268603894
28	0.03875361875268434
29	0.2695377894117288
30	0.01781302170498676
31	4.1186177352570537E-4
32	0.14043199409184287
33	0.0027543256104531548
34	0.010373768420678704
35	0.0010682664750822984
36	0.017336806529347663
37	0.031159917303304148
38	0.01447951547551308
39	0.032549950788953404
40	0.008610485202771778
41	0.0019949554655151356
42	0.02762048018706762
43	0.0010682664750822984
44	0.022626656183068437
45	0.026217576020995684
46	0.02450577552477947
47	0.0035780491575045656
48	0.017838763065832115
49	0.012832068381410258
50	0.006795719263174138
51	0.008880769491648023
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7769597.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	5.649891329158516
#Duplication Level	Relative count
1	100.0
2	3.4253707794256862
3	0.5422320395498054
4	0.22457136846534131
5	0.14568212895761018
6	0.1125486483643631
7	0.07941516777111601
8	0.061533606816030297
9	0.043652045860944566
10++	0.5506468917639634
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	995270	3.7433746	4.115018	3
TTTTT	1085750	3.6706464	4.264289	1
>>END_MODULE