##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV21_TGACCA_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7849819	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.46345094071596	31.0	30.0	31.0	25.0	34.0
2	30.346682770647323	31.0	30.0	31.0	27.0	34.0
3	30.723372475212486	31.0	30.0	31.0	27.0	34.0
4	32.52972979376977	35.0	32.0	37.0	25.0	37.0
5	33.17690497067512	35.0	33.0	37.0	28.0	37.0
6	33.39046760186445	35.0	33.0	37.0	28.0	37.0
7	33.39051652018983	35.0	33.0	37.0	28.0	37.0
8	33.59814321833408	35.0	33.0	37.0	28.0	37.0
9	35.05510369092587	37.0	34.0	39.0	29.0	39.0
10	34.955526490483415	37.0	34.0	39.0	28.0	39.0
11	34.93181205834173	37.0	34.0	39.0	28.0	39.0
12	35.10611493080286	37.0	34.0	39.0	29.0	39.0
13	34.84985488200429	37.0	34.0	39.0	27.0	39.0
14	35.67543315329946	38.0	34.0	40.0	27.0	41.0
15	36.08193335413211	38.0	34.0	40.0	30.0	41.0
16	35.73129278522218	38.0	34.0	40.0	27.0	41.0
17	34.99854442503706	38.0	33.0	40.0	27.0	41.0
18	35.39260777859974	38.0	33.0	40.0	27.0	41.0
19	35.32364109287106	38.0	34.0	40.0	27.0	41.0
20	35.511059172192375	38.0	34.0	40.0	27.0	41.0
21	35.05101582597	37.0	33.0	40.0	27.0	41.0
22	35.122669707416186	37.0	33.0	40.0	27.0	41.0
23	35.31708323465802	38.0	34.0	40.0	27.0	41.0
24	35.64143198715792	38.0	34.0	40.0	27.0	41.0
25	35.71516311395205	38.0	34.0	40.0	27.0	41.0
26	35.13393327922593	38.0	34.0	40.0	27.0	41.0
27	34.616001846666784	37.0	33.0	40.0	25.0	40.0
28	34.14804914610133	37.0	32.0	40.0	24.0	40.0
29	34.02550135232418	37.0	32.0	39.0	24.0	40.0
30	34.35491149541155	37.0	32.0	39.0	25.0	40.0
31	34.40461111268935	37.0	33.0	39.0	25.0	40.0
32	34.44040607815288	37.0	33.0	39.0	25.0	40.0
33	34.18304702312244	37.0	32.0	39.0	24.0	40.0
34	34.896766409518484	37.0	33.0	40.0	25.0	40.0
35	34.355561064529006	37.0	33.0	40.0	24.0	40.0
36	34.94993094745242	38.0	33.0	40.0	25.0	40.0
37	34.58934148672727	37.0	33.0	40.0	25.0	40.0
38	34.697157603251746	37.0	33.0	40.0	25.0	40.0
39	34.097524796431614	37.0	32.0	40.0	24.0	40.0
40	33.71962194287537	37.0	31.0	40.0	22.0	40.0
41	34.12162853691276	37.0	32.0	40.0	24.0	40.0
42	34.0948749264155	37.0	32.0	40.0	24.0	40.0
43	33.962203077548665	37.0	32.0	40.0	24.0	40.0
44	33.88481275810309	37.0	32.0	40.0	23.0	40.0
45	33.54361317120815	37.0	31.0	40.0	22.0	40.0
46	33.13763361932294	37.0	30.0	40.0	19.0	40.0
47	33.68724629192087	37.0	31.0	40.0	23.0	40.0
48	33.026800872733496	37.0	30.0	39.0	18.0	40.0
49	33.14396981637411	37.0	30.0	39.0	20.0	40.0
50	33.57767102655488	37.0	31.0	39.0	23.0	40.0
51	32.67120350163488	36.0	30.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	13.0
11	14.0
12	23.0
13	35.0
14	78.0
15	171.0
16	518.0
17	1166.0
18	2501.0
19	5058.0
20	9158.0
21	16162.0
22	26616.0
23	41386.0
24	63270.0
25	92393.0
26	130045.0
27	178658.0
28	237321.0
29	309464.0
30	391665.0
31	483088.0
32	578159.0
33	678069.0
34	776413.0
35	866166.0
36	921866.0
37	908126.0
38	779175.0
39	353026.0
40	11.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.484019838127768	25.674172436562152	32.50032683827736	27.341480887032716
2	19.43684306606305	28.218344907060917	29.861784074257	22.483027952619032
3	21.62890634803172	28.347443425128656	28.211376593524	21.81227363331562
4	21.501795136932454	25.36505618791975	30.497849695642664	22.635298979505134
5	20.805040730901396	27.524244523500812	30.00863459949497	21.662080146102824
6	21.590778080360835	28.30467810786465	27.730728568391193	22.37381524338332
7	21.715991158522254	27.965689399972156	29.061256062082453	21.257063379423144
8	21.052090500430648	27.90629949556799	28.682024388078247	22.35958561592312
9	20.898239819287554	27.397510694195624	29.44215656437429	22.262092922142536
10	21.50263592064989	27.612407368883286	28.10041862111725	22.784538089349578
11	21.226922557067876	27.53932288120274	28.352105953016242	22.881648608713142
12	21.7036978814416	27.84339358652728	27.743251150122063	22.70965738190906
13	21.307370271849578	27.943204805104422	28.212675986541857	22.536748936504143
14	21.582930765664788	28.098278444381965	27.836399794695904	22.482390995257344
15	21.68393946408191	27.936491274512186	27.83786479662779	22.541704464778107
16	21.410929495819257	27.89633983217925	28.175760821544078	22.516969850457414
17	21.16488632957585	27.799099407753864	28.496516658787012	22.539497603883273
18	21.54687897899302	27.833227747034677	28.120049647004603	22.499843626967706
19	21.12168589070129	27.693119666988075	28.685220239116642	22.49997420319399
20	21.473909143637577	27.624828037436277	28.306525284213556	22.594737534712582
21	21.166117996331778	27.557119913248897	28.66335702164051	22.613405068778814
22	21.173974734914314	27.610963745740936	28.509479184368985	22.70558233497577
23	21.414922020568092	27.673353942362155	28.323983003538768	22.58774103353099
24	21.84557376418488	27.725581952908723	27.8831524650441	22.5456918178623
25	21.843109588172034	27.66747255384705	27.99060452239528	22.498813335585638
26	21.791182108886925	27.755893814806587	28.06096725302823	22.391956823278257
27	21.876766059345073	27.610907093316232	28.07952008932127	22.432806758017424
28	21.725574838805795	27.581531903472477	28.305336674232162	22.38755658348957
29	21.851269069386596	27.496712053571997	28.15398193501173	22.498036942029678
30	22.103928110047374	27.55744566773386	27.81467239714339	22.523953825075377
31	22.02505060964059	27.54202692581928	27.96161782182935	22.47130464271078
32	22.04634758314274	27.637483787123756	27.917061010938383	22.39910761879512
33	22.10783533741109	27.526189621124903	27.90540741102166	22.460567630442345
34	22.029047142426144	27.66010659333491	27.880303166203248	22.4305430980357
35	21.810858803552684	27.60410781374069	28.282106260310076	22.30292712239655
36	22.17840271261905	27.747288731550913	27.76038701608337	22.313921539746666
37	21.884758694504985	27.6086828256152	28.202616765358307	22.303941714521507
38	22.105580344060485	27.62923928028112	27.885373479669042	22.37980689598936
39	21.918400703643908	27.520242696999205	28.260982871363122	22.300373727993765
40	21.799439858419202	27.42866781830804	28.39327682315153	22.378615500121224
41	22.146724106214243	27.570129295035546	27.95749718076387	22.325649417986345
42	22.06782489510675	27.523204199247097	28.031327675991673	22.37764322965448
43	21.919801307790372	27.533307200796358	28.18541888516151	22.361472606251755
44	21.998118992278336	27.561133706621156	28.095657435899263	22.345089865201242
45	21.96178128704123	27.473732981262984	28.235589296905044	22.328896434790742
46	21.750958165887383	27.465090243056466	28.49011558134847	22.293836009707682
47	22.154025684323976	27.63063034944108	27.85647812258943	22.358865843645514
48	21.674784141014065	27.406028952785288	28.658441100901534	22.260745805299113
49	21.91193710167924	27.466427375329843	28.323355711897886	22.298279811093032
50	22.144615452823693	27.52643625677739	27.963356536375795	22.36559175402312
51	22.009802666271206	27.397039325200666	28.188226684650918	22.404931323877204
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.82550072516048
2	41.91987101868209
3	43.44117998134734
4	44.137094116437595
5	42.46712087700422
6	43.96459332374415
7	42.9730545379454
8	43.41167611635377
9	43.16033274143009
10	44.28717400999946
11	44.108571165781015
12	44.41335526335066
13	43.844119208353725
14	44.06532176092213
15	44.225643928860016
16	43.927899346276675
17	43.70438393345913
18	44.046722605960724
19	43.62166009389527
20	44.06864667835016
21	43.779523065110595
22	43.87955706989008
23	44.00266305409908
24	44.39126558204718
25	44.34192292375767
26	44.183138932165186
27	44.30957281736249
28	44.11313142229537
29	44.349306011416274
30	44.62788193512275
31	44.49635525235137
32	44.44545520193787
33	44.56840296785344
34	44.45959024046184
35	44.11378592594923
36	44.49232425236572
37	44.188700409026495
38	44.485387240049846
39	44.21877443163768
40	44.178055358540426
41	44.472373524200584
42	44.44546812476123
43	44.28127391404213
44	44.343208857479574
45	44.29067772183197
46	44.044794175595065
47	44.51289152796949
48	43.93552994631318
49	44.21021691277227
50	44.510207206846815
51	44.414733990148406
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	16.5
2	24.0
3	41.0
4	58.0
5	88.5
6	119.0
7	174.5
8	230.0
9	406.5
10	583.0
11	992.5
12	1402.0
13	2329.5
14	3257.0
15	5226.0
16	7195.0
17	10656.0
18	14117.0
19	20383.0
20	26649.0
21	35879.5
22	45110.0
23	58693.5
24	72277.0
25	90958.5
26	134144.0
27	158648.0
28	189302.0
29	219956.0
30	256906.5
31	293857.0
32	333148.5
33	372440.0
34	410171.5
35	447903.0
36	475466.5
37	503030.0
38	522870.0
39	542710.0
40	552438.5
41	562167.0
42	569054.5
43	575942.0
44	580448.5
45	584955.0
46	580195.5
47	575436.0
48	563141.0
49	550846.0
50	525765.5
51	500685.0
52	466401.0
53	432117.0
54	393990.0
55	355863.0
56	315848.5
57	275834.0
58	239996.5
59	204159.0
60	173925.5
61	143692.0
62	120152.0
63	96612.0
64	79659.5
65	62707.0
66	51340.0
67	39973.0
68	32373.5
69	24774.0
70	20189.0
71	15604.0
72	12905.5
73	10207.0
74	8453.5
75	5518.0
76	4336.0
77	3586.5
78	2837.0
79	2375.0
80	1913.0
81	1585.5
82	1258.0
83	1019.5
84	781.0
85	637.5
86	494.0
87	409.0
88	324.0
89	254.5
90	185.0
91	153.0
92	121.0
93	86.0
94	51.0
95	36.0
96	21.0
97	14.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02421711889153113
2	0.0
3	0.0
4	0.0
5	6.369573616920339E-5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.9108720850761018E-4
17	1.3535089153011044
18	0.0
19	1.2739147233840678E-4
20	0.0
21	0.0030064387471864
22	0.001184740692747183
23	0.0023567422382605252
24	0.0
25	0.0010828275148764576
26	0.09484295115594385
27	0.027924210736578768
28	0.04112196727083771
29	0.2897519038337062
30	0.01898132937842261
31	3.8217441701522036E-4
32	0.151761460996744
33	0.003057395336121763
34	0.011758232896834945
35	0.001222958134448705
36	0.018637372403108914
37	0.033758740169677795
38	0.015274237533374974
39	0.033988044819886924
40	0.009325055775171377
41	0.002229350765922119
42	0.029516604140808853
43	0.001146523251045661
44	0.024178901449829607
45	0.027325470816588256
46	0.02564390338172129
47	0.0036179178144107524
48	0.019414460384373194
49	0.013860192190418659
50	0.00714666159818462
51	0.009299577480703695
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7849819.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	2.94914159357996
#Duplication Level	Relative count
1	100.0
2	0.9190517638625775
3	0.35635656136398547
4	0.21197071322512928
5	0.1556499923199017
6	0.10905739593466797
7	0.08192104858942194
8	0.06297680610311812
9	0.0440325636168143
10++	0.46029389176181457
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE