##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV20_CGATGT_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	12933943	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.185311084175954	31.0	30.0	31.0	25.0	34.0
2	30.316302228949052	31.0	30.0	33.0	27.0	34.0
3	30.884603790197623	31.0	30.0	34.0	28.0	34.0
4	33.728885615160046	35.0	35.0	37.0	28.0	37.0
5	33.557084409603476	35.0	33.0	37.0	28.0	37.0
6	33.985402595326114	35.0	35.0	37.0	30.0	37.0
7	34.00178306027791	35.0	35.0	37.0	30.0	37.0
8	34.063161172118974	35.0	35.0	37.0	30.0	37.0
9	35.25416100875039	37.0	34.0	39.0	29.0	39.0
10	34.78449062285183	37.0	34.0	39.0	27.0	39.0
11	34.996874734951284	37.0	34.0	39.0	28.0	39.0
12	35.125032559676505	37.0	34.0	39.0	29.0	39.0
13	35.11788640169514	37.0	34.0	39.0	29.0	39.0
14	36.17618440099821	38.0	35.0	40.0	30.0	41.0
15	36.34764982341425	38.0	35.0	40.0	30.0	41.0
16	36.09109897886515	38.0	34.0	40.0	29.0	41.0
17	35.98184629389506	38.0	34.0	40.0	29.0	41.0
18	36.15620812616849	38.0	34.0	40.0	30.0	41.0
19	36.4342759976598	38.0	35.0	40.0	30.0	41.0
20	35.88452307235311	38.0	34.0	40.0	27.0	41.0
21	35.92436892601119	38.0	34.0	40.0	27.0	41.0
22	36.0422894240372	38.0	34.0	40.0	29.0	41.0
23	35.69251812846245	38.0	34.0	40.0	27.0	41.0
24	35.69913938850666	38.0	34.0	40.0	27.0	41.0
25	35.88032334764426	38.0	34.0	40.0	28.0	41.0
26	35.430629623155134	38.0	34.0	40.0	27.0	41.0
27	35.38076687055138	38.0	34.0	40.0	27.0	41.0
28	35.261275621827004	38.0	34.0	40.0	27.0	41.0
29	34.95436720263882	38.0	34.0	40.0	26.0	41.0
30	34.90339349724983	38.0	33.0	40.0	25.0	41.0
31	35.01946080943762	38.0	34.0	40.0	26.0	41.0
32	34.83779370297209	38.0	33.0	40.0	25.0	41.0
33	34.87508024428436	38.0	33.0	40.0	25.0	41.0
34	35.48685980756216	38.0	34.0	40.0	27.0	41.0
35	35.28268548887219	38.0	34.0	40.0	26.0	41.0
36	35.311863443344386	38.0	34.0	40.0	26.0	41.0
37	35.24747093751689	38.0	34.0	40.0	26.0	41.0
38	35.29783748080535	38.0	34.0	40.0	26.0	41.0
39	35.196642276837004	38.0	34.0	40.0	26.0	41.0
40	35.067692968803094	38.0	34.0	40.0	25.0	41.0
41	34.85628767654226	38.0	33.0	40.0	25.0	41.0
42	34.9780243348838	38.0	33.0	40.0	25.0	41.0
43	35.00975928222353	38.0	33.0	40.0	25.0	41.0
44	35.03866206925452	38.0	33.0	40.0	25.0	41.0
45	34.88654016799053	38.0	33.0	40.0	25.0	41.0
46	34.81335668480988	38.0	33.0	40.0	25.0	41.0
47	34.92904916930591	38.0	33.0	40.0	25.0	41.0
48	34.655758649933745	38.0	33.0	40.0	24.0	41.0
49	34.50474306249842	37.0	33.0	40.0	24.0	41.0
50	34.47283106164918	37.0	33.0	40.0	24.0	41.0
51	33.44768119049233	37.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	22.0
11	36.0
12	38.0
13	52.0
14	63.0
15	175.0
16	546.0
17	1152.0
18	2551.0
19	5321.0
20	10364.0
21	18087.0
22	30729.0
23	49702.0
24	76480.0
25	114466.0
26	165418.0
27	232329.0
28	314825.0
29	417016.0
30	537852.0
31	676980.0
32	829415.0
33	993022.0
34	1173112.0
35	1361288.0
36	1534998.0
37	1634089.0
38	1660133.0
39	1093623.0
40	45.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.436516849146642	26.21252336051259	34.32123763086087	24.0297221594799
2	18.647190574444313	30.01468307073875	30.04900361784492	21.289122736972015
3	19.190342492750467	29.70908009610066	31.568560170923966	19.532017240224903
4	19.367311267724002	27.918694245057367	32.44583650940784	20.268157977810787
5	19.37276721590686	29.4386933738312	31.5498624498413	19.638676960420646
6	19.99408069140246	30.267196940639064	29.385385415723576	20.35333695223491
7	19.724039297219726	30.299236667426165	30.910867629461485	19.06585640589262
8	19.694125758865646	30.483496022829232	30.115487597247025	19.706890621058097
9	19.868966075280685	29.27630576356495	30.63640893959125	20.21831922156312
10	19.87677297835621	29.898693578617248	29.458317611327267	20.766215831699274
11	19.613477498702444	29.7899488191652	30.02678301582124	20.569790666311118
12	19.366923849728547	29.89503324491119	29.795171693201556	20.942871212158703
13	19.13748189550549	30.32090059466011	30.23383511122633	20.307782398608065
14	19.377455227862388	30.044408171066365	29.95631396330305	20.6218226377682
15	19.235804580242856	30.554340621417612	29.775568053763653	20.43428674457588
16	19.305057962993803	29.924681895125243	30.030434650163112	20.739825491717838
17	19.747164495776733	29.9107240537553	30.053186410362255	20.288925040105713
18	19.50978078739885	29.944499459097035	30.092490077260365	20.453229676243755
19	19.96469133057274	29.73933530200479	29.61950323853516	20.676470128887313
20	19.594324354185026	29.577253748648225	30.35541636265361	20.47300553451314
21	20.10461358561704	29.600787648199166	29.84388140340118	20.450717362782616
22	19.685137385141907	30.0828172797135	29.94343158035534	20.288613754789257
23	19.15425755147068	30.502625220237377	30.116519759278265	20.22659746901368
24	19.421494280591773	29.856711135962172	29.955219378962784	20.766575204483274
25	19.69065620933629	29.697653363311566	30.49280066306732	20.118889764284827
26	19.481470792170246	29.95559776974223	30.015516342968716	20.5474150951188
27	19.734131433475937	29.658979701592546	29.477747511402224	21.129141353529295
28	20.14767241511984	30.191022272591123	29.515697802159984	20.145607510129054
29	20.411355374342904	29.453112778388903	29.881520986097005	20.25401086117119
30	19.86711297956391	29.399457260585756	30.15369677427936	20.579732985570974
31	19.68500692171408	29.87739616752365	29.66050161841698	20.77709529234529
32	19.61582475676963	29.916054419935996	30.259086001601897	20.20903482169248
33	19.604896604464944	29.758526104396864	29.922159641045358	20.71441765009283
34	19.730405736073177	29.91522656420417	29.540951609325084	20.813416090397574
35	20.07583752896116	29.79278477268148	29.686009465812653	20.445368232544713
36	19.652324490646663	30.50469791329895	29.645534478930973	20.19744311712341
37	19.644821721034784	29.893845718154218	30.42781878494466	20.03351377586634
38	20.56838513941952	29.617863475516604	29.557400425978418	20.25635095908546
39	20.07527033775471	29.583313263356576	30.063523699285742	20.277892699602972
40	19.935513153692877	29.95715568160818	29.559618244055653	20.547712920643292
41	20.045536705463896	29.45920059330084	30.39271233336802	20.10255036786724
42	20.222012203735595	29.59614259459704	29.587590995088398	20.594254206578963
43	19.706508660900827	29.8265701366814	30.337175079948974	20.1297461224688
44	19.7434468192467	29.64338841892078	29.651452968098173	20.96171179373435
45	20.210839125367748	29.66276812644904	29.884508297723357	20.241884450459857
46	19.707054003572345	29.684811312125497	30.168131922425918	20.44000276187624
47	19.80209450472242	30.095087152203703	29.739721471437857	20.363096871636017
48	19.666667620265795	29.613575086691824	30.44222437976173	20.27753291328065
49	20.19345502276651	29.74243008136066	29.96748944872324	20.096625447149595
50	19.81694396443108	29.677295192200408	29.89664727429	20.60911356907851
51	19.792710314798942	29.70040819898993	29.695467502043932	20.811413984167192
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.466239008626545
2	39.93631331141633
3	38.72235973297537
4	39.63546924553479
5	39.0114441763275
6	40.34741764363737
7	38.78989570311235
8	39.40101637992374
9	40.0872852968438
10	40.642988810055485
11	40.183268165013565
12	40.30979506188725
13	39.44526429411356
14	39.99927786563059
15	39.670091324818735
16	40.044883454711645
17	40.03608953588245
18	39.963010463642604
19	40.64116145946005
20	40.06732988869817
21	40.55533094839966
22	39.97375113993116
23	39.380855020484354
24	40.18806948507505
25	39.80954597362112
26	40.02888588728905
27	40.863272787005236
28	40.293279925248896
29	40.66536623551409
30	40.446845965134884
31	40.46210221405937
32	39.82485957846211
33	40.319314254557774
34	40.54382182647075
35	40.52120576150587
36	39.849767607770076
37	39.67833549690112
38	40.82473609850498
39	40.35316303735768
40	40.48322607433617
41	40.14808707333113
42	40.81626641031456
43	39.836254783369625
44	40.705158612981045
45	40.45272357582761
46	40.14705676544858
47	40.165191376358436
48	39.94420053354644
49	40.2900804699161
50	40.42605753350959
51	40.604124298966134
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	259.0
1	416.5
2	574.0
3	893.0
4	1212.0
5	1884.0
6	2556.0
7	3839.5
8	5123.0
9	7261.5
10	9400.0
11	13619.0
12	17838.0
13	25080.5
14	32323.0
15	43287.5
16	54252.0
17	71189.0
18	88126.0
19	110994.5
20	133863.0
21	163973.5
22	194084.0
23	231111.5
24	268139.0
25	312074.0
26	409545.0
27	463081.0
28	526294.5
29	589508.0
30	658973.0
31	728438.0
32	797411.5
33	866385.0
34	920387.5
35	974390.0
36	988559.5
37	1002729.0
38	994886.0
39	987043.0
40	951095.0
41	915147.0
42	893849.5
43	872552.0
44	830306.0
45	788060.0
46	750503.0
47	712946.0
48	676241.5
49	639537.0
50	596303.0
51	553069.0
52	524772.0
53	496475.0
54	420622.0
55	344769.0
56	297898.0
57	251027.0
58	215337.0
59	179647.0
60	152034.0
61	124421.0
62	104428.0
63	84435.0
64	70906.5
65	57378.0
66	48443.5
67	39509.0
68	33513.0
69	27517.0
70	23799.5
71	20082.0
72	17626.5
73	15171.0
74	13231.5
75	9769.0
76	8246.0
77	7118.0
78	5990.0
79	5057.5
80	4125.0
81	3460.5
82	2796.0
83	2343.5
84	1891.0
85	1513.5
86	1136.0
87	908.5
88	681.0
89	513.0
90	345.0
91	281.5
92	218.0
93	157.5
94	97.0
95	66.0
96	35.0
97	24.5
98	14.0
99	8.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.8896988335266363
2	0.0
3	3.865797150953889E-5
4	0.0
5	0.9845566815935403
6	0.0
7	0.0
8	0.0
9	0.05341758503188084
10	0.006981629654622724
11	0.0
12	0.01576472078158996
13	0.0
14	4.793588467182823E-4
15	0.0
16	7.731594301907778E-6
17	0.0
18	1.1597391452861668E-4
19	6.958434871717001E-5
20	1.082423202267089E-4
21	0.0019328985754769447
22	3.711165264915734E-4
23	0.0013221026256262302
24	0.0
25	8.73670156115579E-4
26	0.023666410158139712
27	0.02759406006350886
28	0.027570865280603138
29	0.36288237856004163
30	0.019383106914882802
31	6.185275441526222E-5
32	0.1296201784714839
33	0.0012679814655128757
34	0.01138090681240825
35	0.0018633142267597746
36	0.013236489444866118
37	0.02428493770229233
38	0.0032781959840088985
39	2.3194782905723335E-4
40	0.008296000685947047
41	0.0015540504546834634
42	0.005334800068316367
43	5.334800068316367E-4
44	0.006154349064318591
45	0.009579445340063737
46	0.005458505577146892
47	0.014782808305247672
48	0.0037575548307271804
49	0.007043482409037986
50	0.006602781533829243
51	0.004213718894539739
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.2933943E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	39.937112984432574
#Duplication Level	Relative count
1	100.0
2	39.933678057553955
3	12.089703237410072
4	3.296023895169579
5	1.1345387975334018
6	0.5620503597122302
7	0.4062821171634121
8	0.3332155704008222
9	0.2529226618705036
10++	2.5774023638232273
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC	36733	0.28400465349197845	TruSeq Adapter, Index 2 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA	17519	0.13544980057512238	No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC	15104	0.1167780003360151	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACAC	1330635	2.8951218	9.067362	12
CCAGG	834430	2.865789	7.161762	35
CCCAG	795905	2.6230245	6.865413	34
CAGGG	702595	2.5146208	6.363669	6
CCCTG	725560	2.3579075	5.994901	38
GGGGA	569110	2.1226425	6.822893	19
CTCCC	678730	2.1165926	6.2107844	44
CAGGC	616240	2.1164315	6.5833545	36
TCCAG	943800	2.11015	8.255485	25
GGAAG	825845	2.0335083	8.811857	5
CTGAA	1233075	1.8967183	6.3255773	19
CTCCA	861420	1.8481411	7.8981724	24
GCACA	728925	1.6527379	8.013619	11
AGAGC	683240	1.6143868	8.050581	8
GAAGA	986755	1.6040709	6.0969043	6
AAGAG	950495	1.5451266	5.8921103	7
GAGCA	647340	1.5295608	7.9336004	9
TCTGA	970695	1.4723412	5.7015157	18
GTCTG	632560	1.4533179	7.7689056	17
CCAGT	635850	1.4216348	7.531525	26
AGCAC	622385	1.4111729	7.738184	10
CAGTC	575430	1.2865477	7.37686	27
ATGCC	546505	1.221877	7.238317	47
TCACC	546725	1.1729759	7.259432	30
ACTCC	542355	1.1636004	7.391007	23
AGTCA	719895	1.1073438	5.2998824	28
AACTC	746915	1.1024816	5.372021	22
GTCAC	487090	1.089037	7.266795	29
GATGT	671315	1.0611218	5.4026	35
ATCTC	725330	1.0557184	5.1901603	40
GAACT	664670	1.0223968	5.30242	21
TGAAC	623505	0.9590767	5.246485	20
CACGT	417365	0.9331456	7.242962	14
CGGGG	162330	0.88003415	7.6583414	18
GTATG	513845	0.8122149	5.016237	45
GGCGG	142445	0.7722321	7.646377	38
ACGTC	314245	0.7025897	6.9693046	15
CACCG	170450	0.5617436	9.659985	31
CGGAA	237125	0.5602885	7.226479	4
TCTCG	253925	0.5598235	6.764032	41
AGGCG	139150	0.49802446	5.0129223	37
ACACG	207620	0.47074994	6.897584	13
CGATG	182275	0.42469165	6.7204123	34
CGTCT	180245	0.39738268	6.5905848	16
CTCGT	174475	0.38466164	6.5049624	42
CCGAT	154990	0.34652698	6.390657	33
TCGGA	147995	0.34482092	6.934999	3
ACCGA	148855	0.33750835	6.498005	32
ATCGG	129530	0.30179837	6.9063096	2
GATCG	124560	0.29021856	6.9161882	1
>>END_MODULE