##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV19_TGACCA_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	15948282	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.05620780971894	31.0	30.0	31.0	25.0	34.0
2	30.209064524943816	31.0	30.0	33.0	26.0	34.0
3	30.78928583028567	31.0	30.0	34.0	27.0	34.0
4	33.650664943095435	35.0	33.0	37.0	28.0	37.0
5	33.503490595413346	35.0	33.0	37.0	28.0	37.0
6	33.90647406410295	35.0	33.0	37.0	29.0	37.0
7	33.888053020381754	35.0	33.0	37.0	29.0	37.0
8	33.96983562242002	35.0	33.0	37.0	30.0	37.0
9	35.12577392348593	37.0	34.0	39.0	29.0	39.0
10	34.66215602407833	37.0	33.0	39.0	27.0	39.0
11	34.86838569822129	37.0	34.0	39.0	27.0	39.0
12	34.98931715654388	37.0	34.0	39.0	28.0	39.0
13	34.97365013987086	37.0	34.0	39.0	27.0	39.0
14	36.01117217515968	38.0	34.0	40.0	29.0	41.0
15	36.187199912818194	38.0	34.0	40.0	30.0	41.0
16	35.920175916126894	38.0	34.0	40.0	29.0	41.0
17	35.79174314825885	38.0	34.0	40.0	27.0	41.0
18	35.94114174805788	38.0	34.0	40.0	29.0	41.0
19	36.23543200452563	38.0	34.0	40.0	30.0	41.0
20	35.688652796583355	38.0	34.0	40.0	27.0	41.0
21	35.69312594296991	38.0	34.0	40.0	27.0	41.0
22	35.79672650634093	38.0	34.0	40.0	27.0	41.0
23	35.44729946460691	38.0	34.0	40.0	27.0	41.0
24	35.46483025569776	38.0	34.0	40.0	27.0	41.0
25	35.6273634363877	38.0	34.0	40.0	27.0	41.0
26	35.15136508120436	38.0	34.0	40.0	27.0	41.0
27	35.06080811713763	38.0	33.0	40.0	27.0	41.0
28	34.93336881050887	38.0	33.0	40.0	26.0	41.0
29	34.622578908499364	37.0	33.0	40.0	25.0	40.0
30	34.52168848030151	37.0	33.0	40.0	25.0	40.0
31	34.63631229997062	37.0	33.0	40.0	25.0	40.0
32	34.41779308893585	37.0	33.0	40.0	24.0	40.0
33	34.44670385186317	37.0	33.0	40.0	24.0	40.0
34	35.107124014987946	38.0	34.0	40.0	26.0	41.0
35	34.895047378770954	38.0	33.0	40.0	25.0	41.0
36	34.9382665794347	38.0	33.0	40.0	25.0	41.0
37	34.86509499894722	38.0	33.0	40.0	25.0	41.0
38	34.88693114405677	38.0	33.0	40.0	25.0	41.0
39	34.77233140221624	38.0	33.0	40.0	25.0	41.0
40	34.60648005847903	37.0	33.0	40.0	25.0	41.0
41	34.39814188136377	37.0	33.0	40.0	24.0	41.0
42	34.532129855742454	37.0	33.0	40.0	24.0	41.0
43	34.54790616318422	37.0	33.0	40.0	24.0	41.0
44	34.577902184072244	37.0	33.0	40.0	24.0	41.0
45	34.37768594761492	37.0	33.0	40.0	24.0	41.0
46	34.245172614830864	37.0	33.0	40.0	24.0	41.0
47	34.356685817318755	37.0	33.0	40.0	24.0	41.0
48	34.063290578884924	37.0	32.0	40.0	23.0	40.0
49	33.8767350614944	37.0	32.0	40.0	23.0	40.0
50	33.85302868359112	37.0	32.0	40.0	23.0	40.0
51	32.83612517009669	36.0	30.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	11.0
11	19.0
12	34.0
13	55.0
14	143.0
15	422.0
16	1062.0
17	2433.0
18	5164.0
19	9930.0
20	18076.0
21	30917.0
22	50189.0
23	78909.0
24	117527.0
25	171145.0
26	240591.0
27	329139.0
28	438063.0
29	569130.0
30	722016.0
31	888856.0
32	1069581.0
33	1261972.0
34	1464094.0
35	1671717.0
36	1844592.0
37	1927407.0
38	1897239.0
39	1137806.0
40	38.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.495468983342924	26.03503345982029	30.256343983092158	23.213153573744624
2	20.60343552992103	28.621408876517236	28.930338703566942	21.844816889994796
3	21.425945976856685	27.85980127256388	29.94598287614285	20.768269874436584
4	21.481580273035053	26.672283572613026	30.132192295069775	21.713943859282146
5	21.630751271351322	28.30317795424781	29.06352035010232	21.00255042429855
6	21.933824717535387	28.5869743579261	27.908142576620016	21.571058347918502
7	21.078295455272237	29.26813057356272	28.726855971069487	20.92671800009556
8	21.029406176790705	29.281981595258976	28.327960340806612	21.360651887143707
9	21.638609400815643	27.948321491732436	28.65353542000707	21.759533687444847
10	21.02532215406771	28.801416117789756	28.083951218958543	22.08931050918399
11	21.660420852854244	28.051272231077927	28.42037781875189	21.867929097315937
12	20.942368713853377	28.01440477998683	28.22131169438909	22.82191481177071
13	20.80050377840071	29.03414298794064	28.292401651789202	21.872951581869447
14	20.89464248730719	28.334966641696248	28.128730240015127	22.641660630981434
15	20.9106034117029	29.149829429903484	28.071757196166956	21.86780996222665
16	20.96725032622638	28.20862010143915	28.180918056309643	22.64321151602483
17	21.802931500709608	28.171642563129996	28.1727085086657	21.852717427494696
18	21.184455603450033	28.086608848428874	28.849438386832915	21.879497161288178
19	21.347072670134587	28.04762346965386	27.966530039525917	22.63877382068564
20	21.113102410091827	27.85778923258896	29.12820272942423	21.900905627894986
21	22.035080964875455	27.90565442048562	28.18125071129765	21.878013903341273
22	21.074711246917214	28.81063024682115	28.257728695626877	21.85692981063476
23	20.892163350716007	28.93451782805514	28.33614433248521	21.837174488743653
24	21.07016918812948	28.07044043991698	28.122383338844898	22.737007033108643
25	21.27163406115547	28.051858980962898	28.965731686005665	21.71077527187597
26	21.186365736248913	28.181601864556725	28.16236650491069	22.469665894283665
27	21.44784603012468	28.078703201366885	27.82168491950766	22.65176584900077
28	21.56442930815776	28.91895239752823	27.841620920689724	21.67499737362429
29	22.293245042365	27.918446231675055	28.089466978521244	21.698841747438703
30	21.578568572370365	27.814316684536138	28.690997497939197	21.916117245154297
31	21.41912302473898	27.969306016350863	27.950043741415996	22.66152721749416
32	21.238777960008587	28.73971874862838	28.317451749772644	21.704051541590392
33	21.31917208569484	27.98874024305304	28.060090327865524	22.631997343386594
34	21.439518299343536	28.175478746089816	28.703155632699612	21.681847321867036
35	22.15196877998226	28.203853869077903	28.07167406165034	21.57250328928949
36	21.353530512779397	29.0961456587952	27.976202309094177	21.574121519331218
37	21.292397363373862	28.162919367107875	28.11282771010007	22.431855559418192
38	21.547785909104505	28.084279028808197	27.876864670941494	22.491070391145808
39	21.519604766394586	28.867318830624498	27.955349803824735	21.65772659915618
40	21.54867054363242	28.743788724743148	27.949012626632697	21.75852810499174
41	21.532382364528456	27.910410429083438	28.970046871658088	21.587160334730015
42	21.522747466842745	27.970633623222213	27.965284819115737	22.541334090819305
43	21.426431016174558	28.068768910839125	28.868382911044165	21.636417161942152
44	21.371972612343257	28.123911651668337	27.992999455081197	22.511116280907213
45	22.27351845419588	28.051289515198874	28.042014931264923	21.633177099340326
46	21.358730481217698	28.133903455471902	28.82052063463398	21.686845428676417
47	21.412615313625658	28.94600510009771	27.899038308678513	21.74234127759812
48	21.305171605979947	28.011770477691574	29.043167113293357	21.639890803035122
49	22.190163725540668	28.01956853089974	28.126176340139676	21.664091403419917
50	21.413386578757038	27.964175220786785	28.115780954828303	22.506657245627874
51	21.48004423466949	27.882207509660343	28.047930757542545	22.589817498127626
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.70862255708755
2	42.448252419915825
3	42.19421585129327
4	43.195524132317196
5	42.63330169564987
6	43.50488306545389
7	42.005013455367795
8	42.39005806393441
9	43.39814308826049
10	43.1146326632517
11	43.52834995017018
12	43.76428352562409
13	42.673455360270154
14	43.53630311828863
15	42.77841337392956
16	43.61046184225121
17	43.65564892820431
18	43.06395276473821
19	43.98584649082023
20	43.01400803798681
21	43.91309486821673
22	42.93164105755197
23	42.72933783945966
24	43.80717622123812
25	42.98240933303144
26	43.65603163053258
27	44.099611879125455
28	43.23942668178204
29	43.9920867898037
30	43.49468581752466
31	44.08065024223314
32	42.94282950159898
33	43.95116942908144
34	43.12136562121057
35	43.724472069271755
36	42.927652032110615
37	43.724252922792054
38	44.03885630025031
39	43.17733136555077
40	43.30719864862416
41	43.119542699258474
42	44.06408155766206
43	43.06284817811671
44	43.88308889325047
45	43.9066955535362
46	43.04557590989411
47	43.15495659122377
48	42.94506240901507
49	43.85425512896058
50	43.92004382438491
51	44.06986173279711
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	120.0
2	154.0
3	246.0
4	338.0
5	488.0
6	638.0
7	964.0
8	1290.0
9	1980.0
10	2670.0
11	4251.0
12	5832.0
13	8678.0
14	11524.0
15	16743.5
16	21963.0
17	30786.0
18	39609.0
19	53413.5
20	67218.0
21	87207.5
22	107197.0
23	135756.5
24	164316.0
25	203790.0
26	297327.0
27	351390.0
28	430024.5
29	508659.0
30	611899.0
31	715139.0
32	827309.0
33	939479.0
34	1035681.5
35	1131884.0
36	1168820.0
37	1205756.0
38	1205498.5
39	1205241.0
40	1159149.5
41	1113058.0
42	1090624.0
43	1068190.0
44	1044347.0
45	1020504.0
46	998039.5
47	975575.0
48	950291.5
49	925008.0
50	891252.5
51	857497.0
52	829050.0
53	800603.0
54	697420.0
55	594237.0
56	525331.0
57	456425.0
58	401754.0
59	347083.0
60	303430.5
61	259778.0
62	226960.0
63	194142.0
64	170452.0
65	146762.0
66	129939.0
67	113116.0
68	99214.5
69	85313.0
70	76571.0
71	67829.0
72	60740.0
73	53651.0
74	48000.0
75	37389.0
76	32429.0
77	28152.0
78	23875.0
79	20567.0
80	17259.0
81	14406.5
82	11554.0
83	9611.5
84	7669.0
85	6244.5
86	4820.0
87	3833.5
88	2847.0
89	2216.5
90	1586.0
91	1190.5
92	795.0
93	608.5
94	422.0
95	303.0
96	184.0
97	120.5
98	57.0
99	37.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.1073503716575868
2	0.0
3	3.7621607142386874E-5
4	0.0
5	0.9314984523097848
6	6.270267857064478E-6
7	0.0
8	0.0
9	0.048462900267251356
10	0.006401943482062833
11	0.0
12	0.015449939999806877
13	0.0
14	4.953511607080939E-4
15	0.0
16	6.270267857064478E-6
17	0.0
18	1.6929723214074093E-4
19	6.897294642770926E-5
20	6.27026785706448E-5
21	0.0019876749106894397
22	3.3859446428148186E-4
23	0.0013543778571259274
24	0.0
25	6.834591964200281E-4
26	0.023124747856853797
27	0.026836746428235966
28	0.026692530267523485
29	0.36616483204899436
30	0.01897383053547711
31	6.27026785706448E-5
32	0.14304362062321194
33	9.907023214161877E-4
34	0.011167347053431836
35	0.001937512767832924
36	0.012722373481983826
37	0.022014910446153384
38	0.0031037825892469167
39	2.696215178537726E-4
40	0.007994591517757211
41	0.0016616209821220867
42	0.004966052142795067
43	5.455133035646096E-4
44	0.005969294999925384
45	0.009248645089170105
46	0.005154160178507002
47	0.01450312955339014
48	0.0036743769642397844
49	0.0065524299106323805
50	0.00624518678563622
51	0.003824863392809332
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.5948282E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	15.707347761642168
#Duplication Level	Relative count
1	100.0
2	5.444357747848605
3	1.1930205470932138
4	0.7898813764403886
5	0.6044260496884577
6	0.4772082188372856
7	0.4461105268514435
8	0.3409437866811412
9	0.2911874795037939
10++	3.4953805792086485
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC	72489	0.45452544669074707	TruSeq Adapter, Index 4 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GAAAA	3183690	3.1320646	3.4597666	4
TTTTC	3298400	3.0791974	3.64541	2
CACAC	1919320	3.0337892	13.237965	12
CACTG	1509410	2.4376295	12.239217	31
CTGAA	1800630	2.2626133	10.038088	19
TCCAG	1372835	2.2170668	12.076814	25
GGAAG	1215675	2.0877993	12.607973	5
ACTGA	1604170	2.015748	9.740608	32
CTCCA	1228310	1.9295559	11.713528	24
GCACA	1073195	1.7439246	11.894466	11
GAAGA	1333765	1.733664	9.79687	6
AGAGC	965675	1.613211	11.709561	8
AAGAG	1222595	1.5891622	9.44194	7
GAGCA	933070	1.5587426	11.568068	9
TCTGA	1205195	1.5050623	9.212096	18
GTCTG	895700	1.4779013	11.456541	17
CCAGT	901895	1.45652	11.059375	26
TCACT	1194240	1.4507004	8.914417	30
AGCAC	870105	1.4139067	11.477219	10
CTGAC	839980	1.35653	11.173381	33
CAGTC	819250	1.3230519	10.912309	27
CACGT	773555	1.2492566	11.317137	14
GACCA	750320	1.219258	10.888327	35
ACTCC	775545	1.218306	11.1918545	23
ATGCC	751575	1.2137599	10.100684	47
AACTC	975955	1.1929014	8.918227	22
TGACC	732200	1.1824701	10.8707485	34
AGTCA	922555	1.1592528	8.547984	28
ACCAA	919990	1.1314791	8.472425	36
GAACT	896755	1.1268333	8.854343	21
ATCTC	923150	1.1213944	8.615064	40
GTCAC	684870	1.1060343	10.822155	29
TGAAC	817085	1.0267226	8.734669	20
ACGTC	559050	0.9028406	10.938102	15
CCAAT	730260	0.89259046	8.1795	37
AATCT	920115	0.86966825	6.591361	39
GTATG	667325	0.8567333	7.691782	45
TATGC	635230	0.79328305	7.4475327	46
CAATC	626525	0.76579607	8.108763	38
CGGAA	441960	0.7383175	11.200039	4
TCTCG	454415	0.7293306	10.543736	41
ACACG	349220	0.5674769	10.71649	13
CGTCT	309285	0.49639872	10.448015	16
CTCGT	291310	0.46754906	9.895157	42
TCGGA	270090	0.44841486	10.906171	3
ATCGG	233040	0.3869029	10.914029	2
GATCG	228910	0.38004613	11.02283	1
CGTAT	237630	0.29675525	7.2514977	44
TCGTA	212925	0.26590335	7.2821865	43
>>END_MODULE