##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename SV18_GCCAAT_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21170751 Filtered Sequences 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.009210254279594 31.0 30.0 31.0 25.0 34.0 2 30.158268924895484 31.0 30.0 31.0 26.0 34.0 3 30.734566903176933 31.0 30.0 33.0 27.0 34.0 4 33.5981760873764 35.0 33.0 37.0 28.0 37.0 5 33.44232318447277 35.0 33.0 37.0 28.0 37.0 6 33.84842620840423 35.0 33.0 37.0 29.0 37.0 7 33.84485807801528 35.0 33.0 37.0 29.0 37.0 8 33.91892696673821 35.0 33.0 37.0 30.0 37.0 9 35.02373548297838 37.0 34.0 39.0 28.0 39.0 10 34.55543126457819 37.0 33.0 39.0 27.0 39.0 11 34.790521366011056 37.0 33.0 39.0 27.0 39.0 12 34.911615605889466 37.0 34.0 39.0 27.0 39.0 13 34.89747694826698 37.0 34.0 39.0 27.0 39.0 14 35.9162806269839 38.0 34.0 40.0 29.0 41.0 15 36.09162905935647 38.0 34.0 40.0 29.0 41.0 16 35.82787483542742 38.0 34.0 40.0 27.0 41.0 17 35.70355293489589 38.0 34.0 40.0 27.0 41.0 18 35.86667199477241 38.0 34.0 40.0 28.0 41.0 19 36.13829424378946 38.0 34.0 40.0 29.0 41.0 20 35.57942927957539 38.0 34.0 40.0 27.0 41.0 21 35.589118779962035 38.0 34.0 40.0 27.0 41.0 22 35.70544134216117 38.0 34.0 40.0 27.0 41.0 23 35.34015000223658 38.0 34.0 40.0 27.0 41.0 24 35.33190055468509 38.0 34.0 40.0 27.0 41.0 25 35.49827816689167 38.0 34.0 40.0 27.0 41.0 26 34.99248458403767 38.0 33.0 40.0 26.0 41.0 27 34.87921977826861 37.0 33.0 40.0 26.0 41.0 28 34.78811398802055 37.0 33.0 40.0 25.0 40.0 29 34.46985876882686 37.0 33.0 40.0 25.0 40.0 30 34.38295679732854 37.0 32.0 40.0 24.0 40.0 31 34.467471418467866 37.0 33.0 40.0 25.0 40.0 32 34.24317715512312 37.0 32.0 40.0 24.0 40.0 33 34.24307186835271 37.0 32.0 40.0 24.0 40.0 34 34.90969899934112 37.0 33.0 40.0 25.0 40.0 35 34.71343317013175 37.0 33.0 40.0 25.0 41.0 36 34.759249447504246 38.0 33.0 40.0 25.0 41.0 37 34.672661210743065 38.0 33.0 40.0 25.0 41.0 38 34.69662054029165 38.0 33.0 40.0 25.0 41.0 39 34.590866757631794 37.0 33.0 40.0 24.0 41.0 40 34.41474711974082 37.0 33.0 40.0 24.0 41.0 41 34.186007289018704 37.0 33.0 40.0 24.0 41.0 42 34.312437900762234 37.0 33.0 40.0 24.0 41.0 43 34.32136568986145 37.0 33.0 40.0 24.0 41.0 44 34.33812881744252 37.0 33.0 40.0 24.0 41.0 45 34.18309903130031 37.0 32.0 40.0 24.0 40.0 46 34.087075748989726 37.0 32.0 40.0 24.0 40.0 47 34.18876538673569 37.0 32.0 40.0 24.0 40.0 48 33.86929476427171 37.0 32.0 40.0 23.0 40.0 49 33.69778105651519 37.0 31.0 40.0 23.0 40.0 50 33.65785701225242 37.0 31.0 40.0 23.0 40.0 51 32.59827665064881 36.0 30.0 39.0 19.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 5.0 10 13.0 11 26.0 12 38.0 13 148.0 14 266.0 15 797.0 16 2075.0 17 4598.0 18 9360.0 19 17749.0 20 30457.0 21 50164.0 22 78722.0 23 118711.0 24 175292.0 25 247942.0 26 343191.0 27 462443.0 28 608830.0 29 783118.0 30 982457.0 31 1203440.0 32 1437147.0 33 1686897.0 34 1941969.0 35 2199992.0 36 2412079.0 37 2494959.0 38 2437211.0 39 1440606.0 40 46.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.751230574080104 27.267787550694457 27.492543352463116 23.488438522762326 2 21.546269189978194 27.798909920578634 28.379493953710007 22.275326935733172 3 22.474471734561682 27.385129359381665 28.77836301398689 21.362035892069763 4 22.329760526681362 26.397769261940685 29.696532730463836 21.575937480914114 5 21.74288046597414 28.305095929429548 28.363665940115656 21.588357664480657 6 21.94410108550235 28.320913131518104 27.17292362467444 22.562062158305107 7 21.773677277674278 28.05842362417847 28.393447166801028 21.774451931346224 8 21.49771635403959 28.19407776323098 27.837727627139913 22.470478255589516 9 21.35692224412933 27.712932606733027 28.230405877781788 22.699739271355856 10 21.545139342408003 27.802967000320795 27.781308305171915 22.87058535209929 11 21.493484099831885 27.722762409325963 28.117618501110332 22.666134989731823 12 21.5195131042439 27.634929280970844 28.035405103183784 22.810152511601473 13 21.50228397660527 27.77580256836425 28.01457066874954 22.70734278628094 14 21.632365967669305 27.743154121291557 27.885563494793765 22.738916416245374 15 21.720467072708 27.763710413485093 27.78462606262763 22.73119645117927 16 21.701676997665317 27.671658884467536 27.839031312587824 22.78763280527932 17 21.78292588675763 27.707326017863043 27.757097516285555 22.752650579093768 18 21.932295658273393 27.65581717206189 27.70267432976433 22.70921283990038 19 22.02058011031456 27.652638557797626 27.616749387786875 22.71003194410094 20 21.78290250358313 27.533514775607483 27.990659937818883 22.6929227829905 21 21.981229296792762 27.481662898893738 27.81683951941989 22.720268284893606 22 21.794285413600615 27.60602022578279 27.88247304612162 22.71722131449497 23 21.676095990634504 27.585111193972818 27.999343614377793 22.739449201014885 24 21.818413527229147 27.595709760130855 27.73884592001484 22.847030792625166 25 22.069039139183584 27.559757399412394 27.747031260332726 22.624172201071296 26 21.97339398229346 27.608992419730416 27.82267738549915 22.594936212476977 27 22.280390199871977 27.590755243055643 27.514723872697445 22.61413068437494 28 22.232949070872372 27.63028006524557 27.5975703427944 22.53920052108766 29 22.31662888614469 27.51154902605232 27.61598056110487 22.555841526698117 30 22.331895207099958 27.453288220254652 27.53462839182539 22.68018818082 31 22.249746064567617 27.503082556978654 27.536916997122823 22.71025438133091 32 22.04727485929482 27.552875749704302 27.85415524032045 22.545694150680426 33 22.16306548437375 27.493444770154476 27.684677844893702 22.658811900578073 34 22.34092622727436 27.62693865908733 27.480711491493015 22.551423622145293 35 22.110488841060963 27.614822569900515 27.752638042559262 22.522050546479257 36 22.195211973943618 27.65318016069182 27.619969806449774 22.53163805891479 37 22.127090067775026 27.679500869352157 27.726383190285482 22.467025872587335 38 22.364868496966615 27.7136196260364 27.463096269537356 22.45841560745962 39 22.32297467726622 27.5572607424412 27.631594609531096 22.488169970761476 40 22.36134934545241 27.58567945116476 27.51559127667946 22.53737992670337 41 22.279453651753062 27.523591924188416 27.733895187521433 22.463059236537084 42 22.2497106713841 27.543980311482922 27.601864929592768 22.60444408754021 43 22.27253197638388 27.547700058344997 27.64118809157444 22.538579873696683 44 22.19672262794411 27.58778708891135 27.624618553194708 22.590871729949832 45 22.374598056537312 27.545947412669573 27.573695913747414 22.505758617045696 46 22.227339622066637 27.570580556415674 27.710861870119917 22.491217951397772 47 22.35435087349486 27.69647900295493 27.415347433862465 22.53382268968775 48 22.180839108898496 27.504913336054805 27.83940658482922 22.47484097021748 49 22.300893441927926 27.512895470341302 27.675947972661298 22.510263115069474 50 22.22552784256358 27.542091193898266 27.678269315334592 22.55411164820356 51 22.306565816503138 27.404982019744274 27.655498224648138 22.63295393910445 >>END_MODULE >>Per base GC content pass #Base %GC 1 45.23966909684243 2 43.821596125711366 3 43.836507626631445 4 43.905698007595475 5 43.3312381304548 6 44.50616324380746 7 43.5481292090205 8 43.96819460962911 9 44.05666151548518 10 44.41572469450729 11 44.15961908956371 12 44.32966561584537 13 44.20962676288621 14 44.371282383914675 15 44.45166352388728 16 44.48930980294464 17 44.5355764658514 18 44.64150849817378 19 44.7306120544155 20 44.47582528657363 21 44.701497581686375 22 44.51150672809559 23 44.41554519164939 24 44.665444319854316 25 44.69321134025488 26 44.56833019477043 27 44.894520884246916 28 44.77214959196003 29 44.87247041284281 30 45.01208338791996 31 44.96000044589853 32 44.59296900997524 33 44.821877384951826 34 44.89234984941965 35 44.63253938754021 36 44.72685003285841 37 44.594115940362364 38 44.823284104426236 39 44.8111446480277 40 44.89872927215578 41 44.74251288829015 42 44.85415475892432 43 44.811111850080565 44 44.78759435789394 45 44.880356673583 46 44.71855757346441 47 44.888173563182605 48 44.65568007911598 49 44.8111565569974 50 44.77963949076714 51 44.939519755607584 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 101.0 1 151.5 2 202.0 3 320.0 4 438.0 5 620.0 6 802.0 7 1198.5 8 1595.0 9 2546.5 10 3498.0 11 5447.5 12 7397.0 13 11106.5 14 14816.0 15 21818.0 16 28820.0 17 40372.5 18 51925.0 19 70106.0 20 88287.0 21 115413.5 22 142540.0 23 179814.5 24 217089.0 25 266712.0 26 379625.0 27 442915.0 28 524220.0 29 605525.0 30 701994.5 31 798464.0 32 903495.0 33 1008526.0 34 1105331.0 35 1202136.0 36 1269455.0 37 1336774.0 38 1381639.5 39 1426505.0 40 1442611.0 41 1458717.0 42 1472068.0 43 1485419.0 44 1485023.0 45 1484627.0 46 1468178.0 47 1451729.0 48 1416069.0 49 1380409.0 50 1318039.5 51 1255670.0 52 1174604.0 53 1093538.0 54 992824.0 55 892110.0 56 797658.0 57 703206.0 58 620878.5 59 538551.0 60 470720.0 61 402889.0 62 349938.5 63 296988.0 64 259096.5 65 221205.0 66 195755.0 67 170305.0 68 152414.0 69 134523.0 70 122933.0 71 111343.0 72 102138.5 73 92934.0 74 85414.0 75 70824.0 76 63754.0 77 56931.5 78 50109.0 79 44090.5 80 38072.0 81 32495.0 82 26918.0 83 22687.0 84 18456.0 85 15184.0 86 11912.0 87 9453.0 88 6994.0 89 5512.0 90 4030.0 91 3085.5 92 2141.0 93 1591.0 94 1041.0 95 729.5 96 418.0 97 273.5 98 129.0 99 79.5 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.081997421820322 2 0.0 3 9.446995999338898E-6 4 0.0 5 0.9251112537292607 6 0.0 7 0.0 8 0.0 9 0.05292207158829652 10 0.006726261151529296 11 0.0 12 0.01502544713694852 13 0.0 14 4.440088119689282E-4 15 0.0 16 0.0 17 0.0 18 1.228109479914057E-4 19 8.974646199371954E-5 20 6.612897199537229E-5 21 0.0019838691598611687 22 2.3617489998347248E-4 23 0.001270620961911082 24 0.0 25 7.132481979500869E-4 26 0.02367889547234295 27 0.027410458892081813 28 0.027349053418086116 29 0.36157432487869706 30 0.01930965982264871 31 7.085246999504174E-5 32 0.14130816615811126 33 0.0011808744999173624 34 0.010949068363233784 35 0.001955528171863152 36 0.012536163691122721 37 0.023570255018350555 38 0.0031600201617788615 39 2.4089839798314194E-4 40 0.008039393595437403 41 0.0017524177578773657 42 0.004964396397652592 43 5.998842459580201E-4 44 0.006381445797553427 45 0.009635935919325676 46 0.005413128707621189 47 0.014557820834981245 48 0.00385909786572994 49 0.007066353007505496 50 0.00669792016353128 51 0.004076378773714735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2.1170751E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 5.822530726864627 #Duplication Level Relative count 1 100.0 2 1.9491939167091012 3 0.5559376131955126 4 0.328102935077616 5 0.24095879552100122 6 0.1937119728698245 7 0.1616891264062492 8 0.14646515021864778 9 0.12021691541243851 10++ 1.2966627994267386 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content pass >>END_MODULE