##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV17_CAGATC_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	17208101	
Filtered Sequences	0	
Sequence length	51	
%GC	49	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.033473594791197	31.0	30.0	31.0	25.0	34.0
2	30.190534214089052	31.0	30.0	31.0	26.0	34.0
3	30.77442002461515	31.0	30.0	33.0	27.0	34.0
4	33.65357043174026	35.0	33.0	37.0	28.0	37.0
5	33.4711677947497	35.0	33.0	37.0	28.0	37.0
6	33.85171978011984	35.0	33.0	37.0	29.0	37.0
7	33.82956829460729	35.0	33.0	37.0	29.0	37.0
8	33.89268682232862	35.0	33.0	37.0	30.0	37.0
9	35.049172305532146	37.0	34.0	39.0	29.0	39.0
10	34.53508065765072	37.0	33.0	39.0	27.0	39.0
11	34.73143463070097	37.0	33.0	39.0	27.0	39.0
12	34.84756882819319	37.0	34.0	39.0	27.0	39.0
13	34.85995131014166	37.0	34.0	39.0	27.0	39.0
14	35.879611875825226	38.0	34.0	40.0	29.0	41.0
15	36.00218844601156	38.0	34.0	40.0	29.0	41.0
16	35.70124617469412	38.0	34.0	40.0	27.0	41.0
17	35.53586488131375	38.0	34.0	40.0	27.0	41.0
18	35.67882400271826	38.0	34.0	40.0	27.0	41.0
19	35.936044773330885	38.0	34.0	40.0	29.0	41.0
20	35.31269702566251	38.0	34.0	40.0	27.0	41.0
21	35.278107793532826	38.0	33.0	40.0	27.0	41.0
22	35.37429458369636	38.0	34.0	40.0	27.0	41.0
23	34.96101562862747	37.0	33.0	40.0	26.0	41.0
24	34.973276714263825	37.0	33.0	40.0	26.0	41.0
25	35.10923732955775	37.0	33.0	40.0	27.0	41.0
26	34.50542201024971	37.0	33.0	40.0	25.0	41.0
27	34.33693630691731	37.0	32.0	40.0	24.0	40.0
28	34.16876626886372	37.0	32.0	40.0	24.0	40.0
29	33.86799821781613	37.0	32.0	39.0	23.0	40.0
30	33.67824206750065	37.0	31.0	39.0	22.0	40.0
31	33.719809815156246	37.0	31.0	39.0	23.0	40.0
32	33.40701969380584	37.0	31.0	39.0	21.0	40.0
33	33.3723491627577	37.0	31.0	39.0	21.0	40.0
34	34.06931613197761	37.0	32.0	40.0	24.0	40.0
35	33.834594822519925	37.0	31.0	40.0	23.0	40.0
36	33.84849571722063	37.0	31.0	40.0	23.0	40.0
37	33.72247193342252	37.0	31.0	40.0	22.0	40.0
38	33.69282037570561	37.0	31.0	40.0	22.0	40.0
39	33.532823581172615	37.0	31.0	40.0	21.0	40.0
40	33.30755810882328	37.0	31.0	40.0	20.0	40.0
41	33.02881666024624	36.0	30.0	39.0	19.0	40.0
42	33.10180623649292	36.0	30.0	39.0	20.0	40.0
43	33.13293308773583	36.0	30.0	39.0	20.0	40.0
44	33.06283929876981	36.0	30.0	39.0	20.0	40.0
45	32.84081631087591	36.0	30.0	39.0	19.0	40.0
46	32.675687921636445	36.0	30.0	39.0	19.0	40.0
47	32.715617777929126	36.0	30.0	39.0	19.0	40.0
48	32.3610706376026	35.0	30.0	39.0	17.0	40.0
49	32.13174649544421	35.0	29.0	39.0	16.0	40.0
50	32.04194623218448	35.0	29.0	39.0	15.0	40.0
51	30.984298383650817	34.0	27.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	10.0
11	32.0
12	78.0
13	250.0
14	818.0
15	2071.0
16	5020.0
17	11234.0
18	21362.0
19	37110.0
20	58417.0
21	86431.0
22	120729.0
23	164166.0
24	218119.0
25	283941.0
26	365365.0
27	462692.0
28	579178.0
29	710594.0
30	860874.0
31	1025292.0
32	1192576.0
33	1368497.0
34	1545870.0
35	1711802.0
36	1829113.0
37	1836563.0
38	1719166.0
39	990697.0
40	31.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.761708327359848	22.859181646001417	29.55959278974756	28.819517236891173
2	22.375124367296543	25.52033487018701	26.233795350224874	25.870745412291573
3	23.872713881569016	25.076809767122434	26.872040129013676	24.178436222294874
4	23.83124669014902	23.328634577400493	27.672571191905487	25.167547540545005
5	24.107538099614363	25.075338054772306	27.443419756949496	23.373704088663843
6	24.429055179192076	25.83488158686209	25.081503773310466	24.654559460635365
7	24.40682443693235	25.930845013055187	26.407969130353198	23.254361419659265
8	23.897360899962177	26.25318737959523	25.712511798948647	24.13693992149395
9	23.753541720470043	25.222073010110364	26.38066778473037	24.64371748468922
10	23.626175361169572	25.64135585950905	25.512001113503878	25.22046766581749
11	23.963480920991806	25.16898291101383	25.786610620195688	25.080925547798678
12	23.821263066749378	25.30823832404789	25.396802517599067	25.47369609160367
13	23.666208142316226	25.949708221726496	25.524931542417146	24.85915209354013
14	23.889955542259436	25.59083618360199	25.30572505494093	25.213483219197645
15	23.860418996843404	25.924086568297106	25.361990843731103	24.853503591128387
16	23.789144426802235	25.444312536287416	25.50954344119668	25.25699959571367
17	24.27881496046542	25.36734297410272	25.49134852241976	24.862493543012096
18	24.103491335004794	25.322412344706745	25.791650995741072	24.782445324547385
19	24.146833021745408	25.30822274242971	25.289528048172222	25.255416187652656
20	23.880231688686443	25.168183966484136	26.07056761845343	24.881016726375982
21	24.469687794851957	25.160531963782912	25.506410237039184	24.86337000432595
22	23.92196700452009	25.599652348836187	25.54953628109256	24.928844365551154
23	23.81890405537691	25.63481471731435	25.636418632261528	24.909862595047215
24	24.08216339501959	25.182110449026307	25.302815226386688	25.432910929567417
25	24.28653309182524	25.1653811100791	25.730403816694125	24.817681981401538
26	24.166198853509982	25.247623220474207	25.409839781792336	25.176338144223475
27	24.50054712465287	25.21289325038546	25.048880828548718	25.237678796412954
28	24.4558245245894	25.68319910716102	25.124186577770285	24.736789790479293
29	24.926740823941206	25.051232436051308	25.21393405624429	24.808092683763196
30	24.615085504909658	25.010467972421747	25.469070948557558	24.905375574111037
31	24.432094313751755	25.063889988984833	25.16712628562313	25.33688941164028
32	24.22341160315438	25.505672306549837	25.48288380254704	24.78803228774874
33	24.366383160827056	25.049895757035905	25.24701479717776	25.33670628495928
34	24.50947680451122	25.229828906132447	25.017730559312767	25.242963730043567
35	24.310693646825168	25.722291383340796	25.305004019687345	24.662010950146694
36	24.89728658505931	25.256849648460356	25.220896829299605	24.62496693718073
37	24.342003659647546	25.683486928829613	25.32056209610234	24.653947315420503
38	24.62439639207607	25.16956346361302	25.518787691625704	24.68725245268521
39	24.584582965119445	25.13664374544291	25.103397714176996	25.17537557526065
40	24.628678749117203	25.523210077211942	25.060319652897274	24.78779152077358
41	24.521155958673972	25.043998822165257	25.738275102050828	24.696570117109946
42	24.568314827918705	25.064990176214447	25.091200110465284	25.275494885401567
43	24.537269191022528	25.09554418475074	25.531951492116434	24.835235132110302
44	24.509740848636287	25.125776059017014	25.12010393926973	25.244379153076974
45	25.05897790385974	25.100322879032994	25.069689007211483	24.77101020989578
46	24.514736662987577	25.15450552188997	25.562655574844694	24.768102240277763
47	24.65738743219789	25.622492602111897	24.8884751731211	24.83164479256911
48	24.407039403048394	25.06722534022377	25.782794346045385	24.742940910682453
49	24.9162195976402	25.022345702488447	25.27515143935382	24.786283260517532
50	24.502151283895202	24.989379281631464	25.202851362131103	25.305618072342227
51	24.641620235509485	24.847194880102283	25.10081287819903	25.410372006189206
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.58122556425103
2	48.24586977958811
3	48.05115010386389
4	48.99879423069402
5	47.4812421882782
6	49.083614639827445
7	47.661185856591615
8	48.03430082145613
9	48.397259205159266
10	48.84664302698707
11	49.044406468790484
12	49.294959158353045
13	48.525360235856354
14	49.10343876145708
15	48.713922587971794
16	49.04614402251591
17	49.14130850347752
18	48.88593665955218
19	49.40224920939807
20	48.761248415062425
21	49.33305779917791
22	48.85081137007125
23	48.72876665042412
24	49.515074324587005
25	49.104215073226776
26	49.342536997733454
27	49.738225921065826
28	49.192614315068695
29	49.7348335077044
30	49.52046107902069
31	49.76898372539203
32	49.011443890903124
33	49.70308944578633
34	49.752440534554786
35	48.97270459697186
36	49.52225352224004
37	48.99595097506805
38	49.31164884476128
39	49.7599585403801
40	49.41647026989078
41	49.217726075783915
42	49.84380971332027
43	49.37250432313283
44	49.75412000171326
45	49.82998811375552
46	49.28283890326534
47	49.489032224767
48	49.14998031373084
49	49.70250285815773
50	49.807769356237436
51	50.0519922416987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	79.0
1	138.5
2	198.0
3	269.0
4	340.0
5	520.0
6	700.0
7	1054.0
8	1408.0
9	2133.5
10	2859.0
11	4300.0
12	5741.0
13	8742.5
14	11744.0
15	16793.0
16	21842.0
17	29935.5
18	38029.0
19	50842.0
20	63655.0
21	81995.0
22	100335.0
23	125456.5
24	150578.0
25	184321.0
26	260491.5
27	302919.0
28	359075.0
29	415231.0
30	481820.0
31	548409.0
32	618288.5
33	688168.0
34	750766.5
35	813365.0
36	850423.0
37	887481.0
38	907066.5
39	926652.0
40	924234.0
41	921816.0
42	923111.0
43	924406.0
44	922101.0
45	919796.0
46	913501.0
47	907206.0
48	893056.5
49	878907.0
50	859226.5
51	839546.0
52	826040.0
53	812534.0
54	748514.5
55	684495.0
56	645222.0
57	605949.0
58	576235.0
59	546521.0
60	522276.5
61	498032.0
62	479486.5
63	460941.0
64	447623.0
65	434305.0
66	420500.5
67	406696.0
68	392960.0
69	379224.0
70	365259.5
71	351295.0
72	334339.0
73	317383.0
74	297988.5
75	256120.0
76	233646.0
77	210766.5
78	187887.0
79	165929.0
80	143971.0
81	123657.0
82	103343.0
83	87059.5
84	70776.0
85	58196.0
86	45616.0
87	36621.0
88	27626.0
89	21569.0
90	15512.0
91	11754.5
92	7997.0
93	5950.5
94	3904.0
95	2776.5
96	1649.0
97	1118.0
98	587.0
99	365.5
100	144.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.202869392735433
2	0.0
3	1.7433649418956803E-5
4	0.0
5	0.9391100156838922
6	1.1622432945971203E-5
7	0.0
8	0.0
9	0.047227756275953985
10	0.006717766242771355
11	0.0
12	0.014539663615409974
13	0.0
14	4.300300190009345E-4
15	0.0
16	0.0
17	0.0
18	7.554581414881282E-5
19	5.811216472985602E-5
20	5.811216472985602E-5
21	0.001818910756044493
22	2.731271742303233E-4
23	0.001197110593435034
24	0.0
25	6.973459767582722E-4
26	0.022890381687090285
27	0.026301565756732828
28	0.026446846168557473
29	0.3530953241150781
30	0.018834152588946336
31	3.4867298837913606E-5
32	0.13945757291870844
33	0.0013075237064217602
34	0.011227270225808182
35	0.0017143088595307525
36	0.012889278137082063
37	0.021571235547722552
38	0.00309156716362834
39	1.8595892713553925E-4
40	0.00835071807168031
41	0.0014760489841383429
42	0.005142926578592257
43	5.462543484606465E-4
44	0.0065666746144737295
45	0.009629185695737142
46	0.005514844432863336
47	0.014615209429558787
48	0.003928382335738266
49	0.007049005581731534
50	0.0067816896239741966
51	0.004218943159387547
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.7208101E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	11.759621240398408
#Duplication Level	Relative count
1	100.0
2	7.098092036422735
3	1.2712336086559655
4	0.5042634191954048
5	0.28886613086359286
6	0.2504619631765256
7	0.191464256294944
8	0.16419173141572233
9	0.13079680299218557
10++	1.4170581294387425
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC	49234	0.2861094318309731	TruSeq Adapter, Index 7 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2652955	3.2004888	3.467678	13
TTTTT	2727700	3.0536678	3.5147133	1
CACAC	1649675	2.0600798	6.8407583	12
GGAAG	1415000	1.9532465	7.0259066	5
TCCAG	1397080	1.7771317	6.479143	25
CTGAA	1376260	1.7306098	6.3960986	19
CTCCA	1367085	1.681856	6.256872	24
GAAGA	1258295	1.660651	6.4596305	6
CCAGA	1267420	1.6364816	6.3407354	33
AAGAG	1199960	1.5836626	6.2720156	7
AGAGC	1119025	1.4939477	6.3271136	8
TCTGA	1143610	1.4167209	5.98451	18
GAGCA	1018220	1.3593684	6.1357336	9
CACCA	1078340	1.3466085	5.9562483	31
CATCT	1103490	1.3221169	5.7619834	39
GCACA	1014330	1.3096942	6.1659822	11
GTCTG	957660	1.2408592	5.9498224	17
ACCAG	934945	1.2071929	5.906946	32
AGCAC	930725	1.2017441	5.997441	10
CAGAT	935750	1.1766804	5.7905903	34
CCAGT	907685	1.1546052	5.7413516	26
AACTC	923630	1.1232895	5.722442	22
TCACC	907435	1.1163718	5.5994563	30
CAGTC	863335	1.0981907	5.6904693	27
GAACT	861980	1.0839165	5.713288	21
AGTCA	856620	1.0771766	5.5594344	28
ACTCC	865805	1.0651566	5.7329497	23
ATCTC	864880	1.0362326	5.557646	40
ATCAT	821935	0.97350717	5.3109345	37
TCATC	808550	0.9687422	5.38505	38
TGAAC	754990	0.94937956	5.5720367	20
GTCAC	727655	0.9256012	5.5304136	29
AGATC	729510	0.91733915	5.476209	35
ATGCC	706895	0.89919376	5.4055066	47
GATCA	590230	0.7421983	5.300993	36
GTATG	573660	0.7347946	5.2023735	45
CACGT	570895	0.7261972	5.466672	14
CGGAA	527060	0.70364827	5.6929483	4
TCTCG	507545	0.6360344	5.3186035	41
ACGTC	419790	0.5339868	5.2936854	15
ACACG	401085	0.5178775	5.3570733	13
TCGGA	381185	0.50134784	5.4080095	3
CGTCT	399185	0.50024223	5.179295	16
CTCGT	356260	0.44645032	5.071432	42
GATCG	274000	0.3603744	5.2625365	1
ATCGG	262485	0.3452295	5.227152	2
>>END_MODULE