##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SV16_ACAGTG_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	17701344	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.05684896016935	31.0	30.0	31.0	25.0	34.0
2	30.22485399978668	31.0	30.0	33.0	26.0	34.0
3	30.80652649877885	31.0	30.0	34.0	28.0	34.0
4	33.6734294299913	35.0	33.0	37.0	28.0	37.0
5	33.50143073881848	35.0	33.0	37.0	28.0	37.0
6	33.906418066334396	35.0	33.0	37.0	29.0	37.0
7	33.898343990151254	35.0	33.0	37.0	29.0	37.0
8	33.968665712614815	35.0	33.0	37.0	30.0	37.0
9	35.101901188971866	37.0	34.0	39.0	29.0	39.0
10	34.632515022588116	37.0	33.0	39.0	27.0	39.0
11	34.869157844737664	37.0	34.0	39.0	27.0	39.0
12	34.98443248151101	37.0	34.0	39.0	28.0	39.0
13	34.96940695576562	37.0	34.0	39.0	27.0	39.0
14	35.99539159286436	38.0	34.0	40.0	29.0	41.0
15	36.18016637606726	38.0	34.0	40.0	30.0	41.0
16	35.91421306766311	38.0	34.0	40.0	29.0	41.0
17	35.80043340211907	38.0	34.0	40.0	27.0	41.0
18	35.96526133834809	38.0	34.0	40.0	29.0	41.0
19	36.240704265167665	38.0	34.0	40.0	30.0	41.0
20	35.69039825450542	38.0	34.0	40.0	27.0	41.0
21	35.71052791245682	38.0	34.0	40.0	27.0	41.0
22	35.834078756957666	38.0	34.0	40.0	27.0	41.0
23	35.47711269833522	38.0	34.0	40.0	27.0	41.0
24	35.47305357152542	38.0	34.0	40.0	27.0	41.0
25	35.65572947455289	38.0	34.0	40.0	27.0	41.0
26	35.167213235333996	38.0	34.0	40.0	27.0	41.0
27	35.069611889357105	38.0	33.0	40.0	27.0	41.0
28	34.99234668282815	38.0	33.0	40.0	26.0	41.0
29	34.6809971604416	37.0	33.0	40.0	25.0	40.0
30	34.60478933125078	37.0	33.0	40.0	25.0	40.0
31	34.707670841264935	37.0	33.0	40.0	25.0	40.0
32	34.496898653571165	37.0	33.0	40.0	25.0	40.0
33	34.51025424962082	37.0	33.0	40.0	25.0	40.0
34	35.18198894953965	38.0	34.0	40.0	26.0	41.0
35	34.989187261713006	38.0	34.0	40.0	25.0	41.0
36	35.04255529975577	38.0	34.0	40.0	25.0	41.0
37	34.97813505008433	38.0	34.0	40.0	25.0	41.0
38	35.00557652571466	38.0	33.0	40.0	25.0	41.0
39	34.90392458335367	38.0	33.0	40.0	25.0	41.0
40	34.741118471004235	38.0	33.0	40.0	25.0	41.0
41	34.52154282748248	37.0	33.0	40.0	24.0	41.0
42	34.655480849363755	37.0	33.0	40.0	25.0	41.0
43	34.67802348793403	37.0	33.0	40.0	25.0	41.0
44	34.704401880444784	37.0	33.0	40.0	25.0	41.0
45	34.560824703480144	37.0	33.0	40.0	24.0	41.0
46	34.48264030121103	37.0	33.0	40.0	24.0	41.0
47	34.599774627282535	37.0	33.0	40.0	24.0	41.0
48	34.282105867215506	37.0	33.0	40.0	24.0	41.0
49	34.116435057134645	37.0	32.0	40.0	24.0	40.0
50	34.08052021360638	37.0	32.0	40.0	23.0	40.0
51	33.02270985751139	36.0	30.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	10.0
11	24.0
12	23.0
13	40.0
14	118.0
15	273.0
16	737.0
17	1823.0
18	4028.0
19	8610.0
20	16181.0
21	29811.0
22	49725.0
23	79762.0
24	121920.0
25	181040.0
26	256791.0
27	355255.0
28	477859.0
29	622313.0
30	793432.0
31	982545.0
32	1186305.0
33	1402510.0
34	1631011.0
35	1859642.0
36	2059990.0
37	2146285.0
38	2125157.0
39	1308084.0
40	35.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.150684838848344	24.72084796258023	33.8037180931198	26.324749105451634
2	19.91844800033263	27.82428837041978	29.484433498382952	22.77283013086464
3	21.592163939199416	28.201097509946	28.67209567093299	21.5346428799216
4	21.434027834270665	25.783454634857105	30.308523465788813	22.47399406508342
5	20.867029558302878	27.5000379287503	30.20306311446714	21.429869398479678
6	21.535478974674408	28.45549063706635	27.689317132604007	22.319713255655234
7	21.829461084988804	27.9430533636316	29.15034587204226	21.077139679337343
8	21.414407855132357	28.29532604981859	28.471352231785335	21.818913863263717
9	20.742352624278652	27.581141550390072	29.34154783546987	22.33495798986141
10	21.220883310873138	27.646590002000544	28.184448300897824	22.948078386228495
11	21.081280607845372	27.64507598971016	28.504214143287648	22.76942925915682
12	21.36684150985229	27.919935253365168	28.073416961389892	22.63980627539265
13	21.185515630903506	28.070919360699392	28.280406278754878	22.463158729642224
14	21.391686212371717	28.19240521260481	28.069939257383353	22.345969317640126
15	21.34198397590601	28.063428404080504	28.16957288666894	22.425014733344543
16	21.289201834437392	28.06150268849703	28.28653085020682	22.36276462685876
17	21.2897901989815	27.956233153821543	28.317375223033913	22.436601424163047
18	21.558395468993787	27.9987731989735	28.149987893559587	22.292843438473124
19	21.536600835270523	27.93356612562072	28.160656393578538	22.369176645530214
20	21.335316987301525	27.857540728555012	28.422452763463106	22.384689520680357
21	21.440788268353774	27.80482116891062	28.33466197939244	22.41972858334317
22	21.365124518248688	27.873177231436262	28.352435789889118	22.409262460425932
23	21.26379742579117	27.86098151485035	28.47536608966389	22.399854969694584
24	21.44967071426893	27.903468798753362	28.176696639532008	22.470163847445708
25	21.622566586530926	27.91571652002419	28.162869045994864	22.29884784745002
26	21.56406573507081	27.983926685633552	28.2443187632546	22.20768881604104
27	21.859038191537802	27.940142868622097	27.94955148854539	22.25126745129471
28	21.808105071785466	27.989767948541832	27.98808400440108	22.21404297527162
29	21.813701433545248	27.81137653877137	28.108035652166663	22.266886375516716
30	21.972175388431044	27.787070453271678	27.88853995289505	22.352214205402227
31	21.79412188735218	27.842662426231524	28.036444497271617	22.32677118914468
32	21.643553871523075	27.852125558886016	28.27001889557917	22.234301674011743
33	21.757177656340577	27.825326799444127	28.112347747860632	22.305147796354667
34	21.896137760399128	28.01119226404016	27.892414011618943	22.200255963941764
35	21.700155996739394	27.994191970164866	28.170803351288804	22.134848681806933
36	21.80348569247016	28.058035021905432	28.062391191113957	22.076088094510453
37	21.751133320329654	27.96861894376828	28.172019910545842	22.108227825356224
38	21.952026837311227	27.98363683305717	27.938164132213757	22.126172197417844
39	21.943318900318985	27.91872242259968	28.0069305585489	22.131028118532438
40	22.00605561785336	27.87887046772168	27.950367933700072	22.164705980724893
41	21.874245631462923	27.8709306893038	28.153371354025463	22.101452325207813
42	21.916229431204926	27.887738464014717	27.99069649037082	22.205335614409535
43	21.88825276945084	27.925497703467663	28.002277578880996	22.183971948200504
44	21.84808271061336	27.947057686617832	28.040870339614234	22.163989263154576
45	21.95522622655323	27.901470759891296	27.992795074317673	22.150507939237805
46	21.886767956982528	27.98628817531954	28.001169242878515	22.125774624819417
47	21.982897779131797	28.034973535425152	27.82347842113631	22.158650264306743
48	21.80639694022536	27.91817814600029	28.20199258219331	22.073432331581046
49	21.84400329354704	27.87150059214468	28.15827918381773	22.126216930490543
50	21.838661928463683	27.859797402274562	28.0923667097926	22.209173959469155
51	21.905715276965264	27.78633713465951	28.081016793972307	22.226930794402914
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.47543394429998
2	42.69127813119727
3	43.12680681912101
4	43.90802189935408
5	42.296898956782556
6	43.85519223032964
7	42.90660076432614
8	43.23332171839607
9	43.07731061414006
10	44.168961697101636
11	43.850709867002195
12	44.00664778524494
13	43.64867436054573
14	43.73765553001184
15	43.76699870925055
16	43.65196646129615
17	43.72639162314455
18	43.85123890746691
19	43.90577748080074
20	43.72000650798188
21	43.86051685169694
22	43.77438697867462
23	43.663652395485755
24	43.91983456171464
25	43.92141443398094
26	43.77175455111185
27	44.110305642832515
28	44.02214804705709
29	44.08058780906197
30	44.32438959383327
31	44.120893076496856
32	43.877855545534814
33	44.06232545269524
34	44.09639372434089
35	43.835004678546326
36	43.879573786980615
37	43.859361145685874
38	44.078199034729074
39	44.07434701885143
40	44.17076159857826
41	43.97569795667074
42	44.12156504561446
43	44.072224717651345
44	44.01207197376794
45	44.105734165791034
46	44.012542581801945
47	44.14154804343854
48	43.8798292718064
49	43.97022022403758
50	44.047835887932834
51	44.13264607136818
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	62.0
2	90.0
3	129.5
4	169.0
5	252.5
6	336.0
7	651.0
8	966.0
9	1625.5
10	2285.0
11	3854.0
12	5423.0
13	8576.5
14	11730.0
15	17724.5
16	23719.0
17	34108.5
18	44498.0
19	60537.5
20	76577.0
21	99857.5
22	123138.0
23	156140.5
24	189143.0
25	231579.0
26	328502.0
27	382989.0
28	450555.5
29	518122.0
30	597186.5
31	676251.0
32	761542.0
33	846833.0
34	927949.0
35	1009065.0
36	1067732.5
37	1126400.0
38	1169518.0
39	1212636.0
40	1233440.5
41	1254245.0
42	1272282.5
43	1290320.0
44	1295404.5
45	1300489.0
46	1289745.0
47	1279001.0
48	1248132.0
49	1217263.0
50	1162899.0
51	1108535.0
52	1035612.0
53	962689.0
54	872148.5
55	781608.0
56	694656.5
57	607705.0
58	529219.5
59	450734.0
60	384174.5
61	317615.0
62	265989.0
63	214363.0
64	177064.0
65	139765.0
66	114469.0
67	89173.0
68	72523.0
69	55873.0
70	45786.5
71	35700.0
72	29775.0
73	23850.0
74	19960.0
75	13533.0
76	10996.0
77	9288.0
78	7580.0
79	6316.0
80	5052.0
81	4155.0
82	3258.0
83	2678.0
84	2098.0
85	1718.5
86	1339.0
87	1047.5
88	756.0
89	590.5
90	425.0
91	332.0
92	239.0
93	177.5
94	116.0
95	82.5
96	49.0
97	33.5
98	18.0
99	9.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.117887545714043
2	0.0
3	5.649288551197016E-6
4	0.0
5	0.9517356422201614
6	5.649288551197016E-6
7	0.0
8	0.0
9	0.043827180580186455
10	0.00645713681401819
11	0.0
12	0.015919695137273194
13	0.0
14	5.536302780173076E-4
15	0.0
16	2.2597154204788066E-5
17	0.0
18	1.2428434812633438E-4
19	7.344075116556122E-5
20	2.2597154204788066E-5
21	0.002073288898289305
22	2.4291940770147173E-4
23	0.0012202463270585555
24	0.0
25	7.287582231044152E-4
26	0.023642272586759515
27	0.027150480777052864
28	0.027088338602989693
29	0.36876860875648765
30	0.01929232040233781
31	9.038861681915226E-5
32	0.14564430813841028
33	0.0011129098445858123
34	0.011162994177165306
35	0.0018812130875486067
36	0.013032908687611517
37	0.021399505031934298
38	0.0034686631704349685
39	1.6382936798471347E-4
40	0.007999392588494976
41	0.0015874500828863614
42	0.005236890486959634
43	5.140852581589285E-4
44	0.0066266154705541
45	0.010089629352437872
46	0.005654937839748213
47	0.014631657347600273
48	0.00392060625453073
49	0.007219790768429787
50	0.007027714957689089
51	0.004333004318768111
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.7701344E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	4.423383077174878
#Duplication Level	Relative count
1	100.0
2	1.40311549757672
3	0.42446319416337164
4	0.305115352283695
5	0.2163828263644572
6	0.16449246032981515
7	0.1318015297279907
8	0.10326182840893759
9	0.08354348931577363
10++	0.9485558911132559
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE