##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename Undetermined_Undetermined_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4625802 Filtered Sequences 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.00082061445777 34.0 33.0 34.0 31.0 34.0 2 33.30413277524632 34.0 34.0 34.0 31.0 34.0 3 33.432364160852536 34.0 34.0 34.0 31.0 34.0 4 36.68245549636582 37.0 37.0 37.0 35.0 37.0 5 36.651476219691205 37.0 37.0 37.0 35.0 37.0 6 36.663021460927204 37.0 37.0 37.0 35.0 37.0 7 36.6600866184934 37.0 37.0 37.0 35.0 37.0 8 36.64986806612129 37.0 37.0 37.0 35.0 37.0 9 38.523668544395115 39.0 39.0 39.0 38.0 39.0 10 38.5316701838946 39.0 39.0 39.0 38.0 39.0 11 38.51414587135377 39.0 39.0 39.0 37.0 39.0 12 38.509121229140376 39.0 39.0 39.0 37.0 39.0 13 38.512876469853225 39.0 39.0 39.0 37.0 39.0 14 40.15944175734283 41.0 40.0 41.0 38.0 41.0 15 40.14335287156692 41.0 40.0 41.0 38.0 41.0 16 40.11841470949254 41.0 40.0 41.0 38.0 41.0 17 40.102179254537916 41.0 40.0 41.0 38.0 41.0 18 40.072239365195486 41.0 40.0 41.0 38.0 41.0 19 40.1113311810579 41.0 40.0 41.0 38.0 41.0 20 40.08467742458497 41.0 40.0 41.0 38.0 41.0 21 40.043967943288536 41.0 40.0 41.0 38.0 41.0 22 40.01562777654556 41.0 40.0 41.0 38.0 41.0 23 39.95710278995945 41.0 40.0 41.0 38.0 41.0 24 39.84283157817823 41.0 40.0 41.0 38.0 41.0 25 39.84860246936639 41.0 40.0 41.0 38.0 41.0 26 36.9193140994794 41.0 39.0 41.0 33.0 41.0 27 36.85707429760288 41.0 39.0 41.0 33.0 41.0 28 36.770067331027136 41.0 39.0 41.0 32.0 41.0 29 36.514489595533924 40.0 39.0 41.0 30.0 41.0 30 36.56098056077627 40.0 39.0 41.0 30.0 41.0 31 36.64998220849055 41.0 39.0 41.0 30.0 41.0 32 36.356607135368094 41.0 39.0 41.0 26.0 41.0 33 36.51751977278751 41.0 39.0 41.0 26.0 41.0 34 36.55735243315645 41.0 39.0 41.0 30.0 41.0 35 36.7067449060725 41.0 39.0 41.0 30.0 41.0 36 36.60532487123314 41.0 40.0 41.0 30.0 41.0 37 36.65351348803948 41.0 39.0 41.0 30.0 41.0 38 36.566670384940814 41.0 39.0 41.0 30.0 41.0 39 36.54964177022709 41.0 39.0 41.0 29.0 41.0 40 36.429501954471895 41.0 39.0 41.0 27.0 41.0 41 36.34631378515552 41.0 39.0 41.0 26.0 41.0 42 36.41309636685703 41.0 39.0 41.0 26.0 41.0 43 36.35378146319276 41.0 39.0 41.0 26.0 41.0 44 36.27482801901162 41.0 39.0 41.0 25.0 41.0 45 36.11149612542863 41.0 39.0 41.0 23.0 41.0 46 36.1092441916018 40.0 38.0 41.0 22.0 41.0 47 36.08907817498458 40.0 38.0 41.0 23.0 41.0 48 36.066815657047144 40.0 38.0 41.0 24.0 41.0 49 35.93269361723654 40.0 38.0 41.0 22.0 41.0 50 35.88822349075901 40.0 38.0 41.0 21.0 41.0 51 35.09259648380973 40.0 36.0 41.0 19.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 1.0 9 7.0 10 8.0 11 28.0 12 51.0 13 207.0 14 833.0 15 2801.0 16 7402.0 17 18422.0 18 76486.0 19 153578.0 20 80312.0 21 12070.0 22 9893.0 23 29564.0 24 5764.0 25 6606.0 26 8099.0 27 8178.0 28 9804.0 29 11962.0 30 13915.0 31 16301.0 32 19908.0 33 25278.0 34 32269.0 35 48416.0 36 84359.0 37 192023.0 38 617588.0 39 3129164.0 40 4504.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.58412914066796 30.616397696464524 30.04949698681683 21.74997617605068 2 20.040243832312754 30.900717324260746 31.185489564836537 17.873549278589962 3 18.116555788596227 30.09605685673533 31.711322706851696 20.076064647816747 4 19.07066061193281 29.53747263717729 31.13933540605499 20.252531344834907 5 20.251882808645938 29.980984918939463 30.70341099770375 19.06372127471085 6 20.12984991575515 29.77983061099459 29.68501462016749 20.40530485308277 7 20.520138994276017 30.061727674465967 29.89178957508341 19.526343756174604 8 19.805300788922654 29.920108988668343 30.15957016750825 20.11502005490075 9 19.67613399795322 29.378127295547884 30.674572755167645 20.27116595133125 10 19.762259603848154 29.95649186886944 29.901474382172 20.379774145110403 11 19.897522634993887 29.865177973462764 29.988961914063765 20.24833747747958 12 19.837857305608843 29.774361289134294 30.033256935770275 20.354524469486588 13 19.691525923504724 30.18533867208324 29.9149206991566 20.208214705255433 14 19.78569337814286 30.205162261592694 29.939889342431865 20.06925501783258 15 19.65527275054142 29.967235951733347 30.116399275195953 20.261092022529283 16 19.722893457177804 30.009974486586326 30.008720649954324 20.25841140628155 17 19.968924218895086 29.95254317023557 29.923313689190735 20.15521892167861 18 19.84356442407176 29.656781678074417 30.02891174330419 20.470742154549633 19 19.792053768984903 29.805532766285857 29.96181130917058 20.440602155558654 20 19.98964918788929 29.776960320157446 29.901740786955727 20.33164970499753 21 19.93489094579377 29.5966620366126 29.944802605745995 20.523644411847634 22 19.939880257801825 29.81170365287 29.811163124367173 20.437252964960997 23 20.11468892090315 29.711049678343223 29.846331555244067 20.32792984550957 24 20.027318449747312 29.546697475036815 29.849739859368917 20.576244215846952 25 20.01895964104233 29.737321415638785 29.74650938307663 20.49720956024226 26 20.162740195560563 29.60246974445657 29.652334166754706 20.582455893228158 27 20.15899301764536 29.426394501161223 29.69944171178371 20.715170769409706 28 20.185425875825196 29.645863084041103 29.465625786482015 20.703085253651686 29 20.403893000306535 29.625002684266327 29.444289615700374 20.52681469972676 30 20.214882517283478 29.41454321431514 29.596108689301843 20.774465579099534 31 20.107347086652556 29.5867806095086 29.590125265555166 20.715747038283684 32 20.3574278919626 29.596155061929796 29.504200350516435 20.542216695591165 33 20.23034681171515 29.396692169438847 29.56867268332338 20.804288335522617 34 20.18447788240777 29.569287437027818 29.518921521197335 20.72731315936708 35 20.412309275620615 29.505269246624028 29.522429628349794 20.55999184940556 36 20.360505449476225 29.361550327668784 29.465541374408694 20.812402848446297 37 20.30329393857852 29.59280871402889 29.43578941112573 20.66810793626686 38 20.387578166121006 29.57603140501105 29.512425002376375 20.523965426491568 39 20.247607630389364 29.40493351704694 29.619384002715606 20.728074849848095 40 20.235218892858715 29.661628211311275 29.35777797445481 20.745374921375202 41 20.327239214549877 29.669340414767447 29.456398587196887 20.547021783485786 42 20.202972571835108 29.47362436935224 29.58034030210705 20.7430627567056 43 20.250735322277063 29.60969410309594 29.48589141012142 20.653679164505576 44 20.32315037868408 29.662532172810774 29.430255551652827 20.584061896852315 45 20.27518515315466 29.441822517170564 29.520607049552527 20.762385280122253 46 20.323377993069354 29.596006113825947 29.37130974412331 20.709306148981394 47 20.33106692666307 29.613004807345174 29.44778856677101 20.608139699220747 48 20.283484638084282 29.462143331568285 29.482038482704098 20.772333547643335 49 20.16969045753396 29.76932273317725 29.332567003620763 20.728419805668025 50 20.295607124012804 29.685825957877725 29.46460965422904 20.553957263880427 51 20.262005475643395 29.481849063766973 29.530178841426384 20.72596661916324 >>END_MODULE >>Per base GC content pass #Base %GC 1 39.33410531671864 2 37.913793110902716 3 38.19262043641297 4 39.32319195676771 5 39.31560408335679 6 40.53515476883792 7 40.04648275045062 8 39.920320843823404 9 39.94729994928447 10 40.14203374895856 11 40.14586011247347 12 40.192381775095434 13 39.89974062876016 14 39.85494839597544 15 39.9163647730707 16 39.98130486345936 17 40.1241431405737 18 40.3143065786214 19 40.23265592454356 20 40.32129889288682 21 40.45853535764141 22 40.37713322276282 23 40.442618766412714 24 40.60356266559427 25 40.51616920128458 26 40.745196088788724 27 40.87416378705507 28 40.88851112947688 29 40.930707700033295 30 40.98934809638302 31 40.82309412493624 32 40.899644587553766 33 41.03463514723777 34 40.91179104177485 35 40.97230112502617 36 41.172908297922525 37 40.971401874845384 38 40.911543592612574 39 40.97568248023746 40 40.98059381423392 41 40.87426099803566 42 40.94603532854071 43 40.904414486782635 44 40.907212275536395 45 41.03757043327691 46 41.03268414205075 47 40.93920662588382 48 41.05581818572762 49 40.89811026320198 50 40.84956438789323 51 40.98797209480664 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2947.0 1 3462.5 2 3978.0 3 4367.0 4 4756.0 5 5414.5 6 6073.0 7 7828.0 8 9583.0 9 12855.5 10 16128.0 11 21679.0 12 27230.0 13 34248.5 14 41267.0 15 48580.5 16 55894.0 17 62586.0 18 69278.0 19 73052.0 20 76826.0 21 78468.5 22 80111.0 23 82983.5 24 85856.0 25 93261.5 26 113419.5 27 126172.0 28 139303.5 29 152435.0 30 175700.0 31 198965.0 32 221334.5 33 243704.0 34 270205.0 35 296706.0 36 325170.5 37 353635.0 38 369224.0 39 384813.0 40 392500.0 41 400187.0 42 407958.5 43 415730.0 44 406582.5 45 397435.0 46 365069.5 47 332704.0 48 295312.0 49 257920.0 50 219870.0 51 181820.0 52 151876.0 53 121932.0 54 100580.5 55 79229.0 56 62338.5 57 45448.0 58 33777.0 59 22106.0 60 19992.5 61 17879.0 62 12743.5 63 7608.0 64 6154.0 65 4700.0 66 3542.5 67 2385.0 68 1593.0 69 801.0 70 581.5 71 362.0 72 281.0 73 200.0 74 168.0 75 112.0 76 88.0 77 62.5 78 37.0 79 30.5 80 24.0 81 21.0 82 18.0 83 12.0 84 6.0 85 4.5 86 3.0 87 2.5 88 2.0 89 1.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 2.5 96 5.0 97 4.5 98 4.0 99 6.0 100 8.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 1.0933023073620531 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.007241987443474667 18 0.0 19 0.0018807549480068537 20 0.0016861940913164898 21 0.020450507825453837 22 0.015110893202951618 23 0.06187035242753581 24 0.22927916067311138 25 0.004042542244566455 26 7.917589209395473 27 7.811358981642534 28 8.005119977033171 29 8.390869302231268 30 7.642220743559712 31 7.573281346672426 32 9.042669790016951 33 7.937650595507546 34 8.619845812682861 35 7.911925326678487 36 8.6150466448845 37 8.334965482742236 38 8.57511843351704 39 8.357361599134594 40 8.64792742966517 41 8.987241563733164 42 8.355524079932518 43 8.553846446518895 44 8.726724576624767 45 9.011496817200564 46 8.74551050823187 47 8.682624115774951 48 8.509119067353076 49 8.901721258281267 50 8.717147858901008 51 8.437844940185506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 4625802.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Duplicate Percentage 51.341954645693384 #Duplication Level Relative count 1 100.0 2 10.930858553773406 3 2.927557683365316 4 1.3910811005521098 5 0.9332818118110907 6 0.8291472096511163 7 0.8697531551474585 8 0.8252176020224381 9 0.7662734875922639 10++ 11.51375035202735 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content pass >>END_MODULE