##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4664453	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89881021418803	34.0	33.0	34.0	31.0	34.0
2	33.21064227681145	34.0	34.0	34.0	31.0	34.0
3	33.33064069892011	34.0	34.0	34.0	31.0	34.0
4	36.630152988999996	37.0	37.0	37.0	35.0	37.0
5	36.58477939428268	37.0	37.0	37.0	35.0	37.0
6	36.602324645569375	37.0	37.0	37.0	35.0	37.0
7	36.56984816869202	37.0	37.0	37.0	35.0	37.0
8	36.57242081761784	37.0	37.0	37.0	35.0	37.0
9	38.4483865525068	39.0	39.0	39.0	37.0	39.0
10	38.43737582949169	39.0	39.0	39.0	37.0	39.0
11	38.44450764108889	39.0	39.0	39.0	37.0	39.0
12	38.43149689792136	39.0	39.0	39.0	37.0	39.0
13	38.40933802956102	39.0	39.0	39.0	37.0	39.0
14	40.055300374985016	41.0	40.0	41.0	38.0	41.0
15	40.02855661746404	41.0	40.0	41.0	38.0	41.0
16	40.01236050615152	41.0	40.0	41.0	38.0	41.0
17	40.001963788680044	41.0	40.0	41.0	38.0	41.0
18	39.98097740506765	41.0	40.0	41.0	38.0	41.0
19	39.98921759957706	41.0	40.0	41.0	38.0	41.0
20	39.947741567982355	41.0	40.0	41.0	38.0	41.0
21	39.93064331444652	41.0	40.0	41.0	38.0	41.0
22	39.896946330041274	41.0	40.0	41.0	38.0	41.0
23	39.82583574108261	41.0	40.0	41.0	38.0	41.0
24	39.68743580436977	41.0	40.0	41.0	38.0	41.0
25	39.71407579838407	41.0	40.0	41.0	38.0	41.0
26	36.10884470269075	41.0	39.0	41.0	17.0	41.0
27	36.04426456864288	40.0	39.0	41.0	16.0	41.0
28	35.99406146872956	40.0	39.0	41.0	15.0	41.0
29	35.72555838808967	40.0	38.0	41.0	2.0	41.0
30	35.73818066126939	40.0	38.0	41.0	15.0	41.0
31	36.10214402417604	40.0	39.0	41.0	17.0	41.0
32	35.9927680694821	41.0	39.0	41.0	16.0	41.0
33	36.026747830881774	41.0	39.0	41.0	16.0	41.0
34	36.14613460570832	41.0	39.0	41.0	16.0	41.0
35	36.21552687957195	41.0	39.0	41.0	16.0	41.0
36	36.14506181110625	41.0	39.0	41.0	15.0	41.0
37	36.088784258304244	41.0	39.0	41.0	13.0	41.0
38	36.04346104462839	41.0	39.0	41.0	15.0	41.0
39	36.004031126479354	41.0	39.0	41.0	9.0	41.0
40	35.98386777613581	41.0	39.0	41.0	12.0	41.0
41	35.86119208404501	41.0	39.0	41.0	8.0	41.0
42	35.84282937356213	41.0	39.0	41.0	9.0	41.0
43	35.78161705134557	41.0	38.0	41.0	8.0	41.0
44	35.719491653147756	40.0	38.0	41.0	7.0	41.0
45	35.651600519932344	40.0	38.0	41.0	8.0	41.0
46	35.60903389958051	40.0	38.0	41.0	7.0	41.0
47	35.553029047564635	40.0	38.0	41.0	7.0	41.0
48	35.48243706175194	40.0	38.0	41.0	6.0	41.0
49	35.404658166777544	40.0	38.0	41.0	2.0	41.0
50	35.33689545162102	40.0	38.0	41.0	6.0	41.0
51	34.524301134559614	39.0	36.0	41.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	0.0
8	2.0
9	1.0
10	8.0
11	17.0
12	74.0
13	327.0
14	1415.0
15	4443.0
16	11162.0
17	26029.0
18	86087.0
19	206093.0
20	103479.0
21	5627.0
22	3062.0
23	4017.0
24	5903.0
25	6503.0
26	7151.0
27	8942.0
28	11153.0
29	12962.0
30	15705.0
31	19793.0
32	22307.0
33	28566.0
34	38169.0
35	54479.0
36	93223.0
37	210438.0
38	652632.0
39	3021325.0
40	3357.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.669378914000042	30.352613827042973	30.113336133575906	21.864671125381076
2	20.05975834679865	30.808864404893775	31.038666270192884	18.092710978114688
3	18.161915234219318	29.928203800102605	31.61183101212511	20.29804995355297
4	19.141108292869497	29.262273625653425	30.960929395150945	20.635688686326134
5	20.44318241505688	29.679157970562414	30.523009704262776	19.354649910117924
6	20.32083933528755	29.49003881055292	29.438757341964855	20.75036451219468
7	20.601601088058985	29.881102886019008	29.66632957819491	19.850966447727096
8	19.922636159052303	29.76762762964918	29.87467126370445	20.43506494759407
9	19.836645368706684	29.076935709288954	30.443505379944874	20.64291354205949
10	19.863293723829997	29.809926265737914	29.600534081917	20.72624592851509
11	20.070177574948232	29.566232096239364	29.737849218332784	20.62574111047962
12	19.901497560378463	29.475331834193636	29.831193496858045	20.79197710856986
13	19.7965334842049	30.01093590180885	29.646262916573495	20.546267697412752
14	19.85266010826993	29.91681982860584	29.72946667058281	20.50105339254142
15	19.73331063685281	29.775559963837132	29.905950386894244	20.585179012415818
16	19.755542611320127	29.743701994639025	29.776160248586493	20.724595145454355
17	20.117171295326592	29.67681312256764	29.70687023751767	20.4991453445881
18	19.847707759087722	29.390927510685604	29.882131945589336	20.879232784637342
19	19.92803702472138	29.551214393967168	29.67562634223084	20.845122239080617
20	20.111601170425153	29.526457199898466	29.685130935311026	20.676810694365354
21	20.116732960703175	29.28807858635801	29.682562585208004	20.912625867730803
22	20.034830207561967	29.62477880052535	29.548755215633843	20.79163577627884
23	20.1958464435484	29.513239815229646	29.588510902720916	20.702402838501033
24	20.089837826980894	29.278129749167608	29.596678133663012	21.035354290188483
25	20.1002104457952	29.464169330826966	29.582898835087335	20.8527213882905
26	20.311564479473855	29.304127696169413	29.38694334265039	20.99736448170634
27	20.291713324272497	29.167865755264355	29.413334625024813	21.127086295438335
28	20.315434818283354	29.410588447355927	29.222902569346758	21.051074165013954
29	20.56950540977101	29.326819357583865	29.19286221288029	20.91081301976483
30	20.288168963665655	29.14740499805583	29.379257170990442	21.185168867288073
31	20.237333845052092	29.340603873545025	29.247545593742124	21.17451668766076
32	20.459344981737875	29.441846294643227	29.234484303816377	20.864324419802525
33	20.3235064843868	29.155659566560043	29.313220791177706	21.20761315787545
34	20.34511966487456	29.354025864482587	29.172523210579225	21.128331260063625
35	20.519808483171058	29.321002939918223	29.21563189374976	20.94355668316096
36	20.434938598814682	29.159273159452432	29.222017684660624	21.18377055707226
37	20.471103519905046	29.36717072833894	29.17831073886992	20.983415012886095
38	20.51673558797297	29.367752982119487	29.26552649249371	20.84998493741383
39	20.39697958982131	29.194869063015737	29.316319786687327	21.091831560475622
40	20.331481362917433	29.427572780540316	29.15368983171019	21.087256024832055
41	20.385335092354563	29.452796094306695	29.22865174353661	20.933217069802133
42	20.33010796851902	29.190838294747813	29.380283838121574	21.098769898611597
43	20.34922818079121	29.390882131088308	29.1809579630719	21.07893172504858
44	20.497716260472888	29.3699420094814	29.225156064258783	20.907185665786926
45	20.40727072769246	29.21036242759319	29.268983542163635	21.11338330255072
46	20.387508969784697	29.408216596307717	29.174151893031887	21.0301225408757
47	20.466907811311337	29.421169568183565	29.18752053858017	20.92440208192493
48	20.409002696200538	29.265427577606516	29.200254124101395	21.12531560209155
49	20.312365241713184	29.495226727467223	29.05790109426707	21.134506936552523
50	20.432305823907836	29.430118081881883	29.177631367492328	20.959944726717954
51	20.368724325192296	29.274138416757207	29.237035098029246	21.120102160021244
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.53405003938112
2	38.15246932491334
3	38.45996518777229
4	39.77679697919563
5	39.79783232517481
6	41.071203847482224
7	40.45256753578608
8	40.357701106646374
9	40.47955891076617
10	40.58953965234509
11	40.69591868542785
12	40.69347466894832
13	40.34280118161765
14	40.35371350081135
15	40.31848964926863
16	40.48013775677448
17	40.61631663991469
18	40.726940543725064
19	40.773159263802
20	40.78841186479051
21	41.02935882843398
22	40.82646598384081
23	40.898249282049434
24	41.12519211716938
25	40.9529318340857
26	41.3089289611802
27	41.41879961971083
28	41.366508983297315
29	41.48031842953584
30	41.473337830953724
31	41.411850532712855
32	41.3236694015404
33	41.531119642262254
34	41.473450924938184
35	41.46336516633202
36	41.61870915588695
37	41.45451853279114
38	41.366720525386796
39	41.48881115029693
40	41.41873738774949
41	41.3185521621567
42	41.428877867130616
43	41.4281599058398
44	41.40490192625982
45	41.52065403024318
46	41.4176315106604
47	41.39130989323627
48	41.53431829829209
49	41.44687217826571
50	41.39225055062579
51	41.48882648521354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	961.0
1	1065.5
2	1170.0
3	1526.5
4	1883.0
5	2663.5
6	3444.0
7	5490.5
8	7537.0
9	11350.5
10	15164.0
11	21565.0
12	27966.0
13	36046.5
14	44127.0
15	53058.5
16	61990.0
17	68918.0
18	75846.0
19	78919.0
20	81992.0
21	81927.5
22	81863.0
23	82488.5
24	83114.0
25	88852.5
26	105901.5
27	117212.0
28	130362.5
29	143513.0
30	166915.0
31	190317.0
32	214037.0
33	237757.0
34	267381.5
35	297006.0
36	325163.0
37	353320.0
38	370930.0
39	388540.0
40	398474.5
41	408409.0
42	418732.0
43	429055.0
44	418889.0
45	408723.0
46	376381.0
47	344039.0
48	307087.0
49	270135.0
50	229100.5
51	188066.0
52	156926.5
53	125787.0
54	102273.0
55	78759.0
56	62293.5
57	45828.0
58	34009.0
59	22190.0
60	20145.0
61	18100.0
62	12823.5
63	7547.0
64	6017.5
65	4488.0
66	3406.0
67	2324.0
68	1542.0
69	760.0
70	547.0
71	334.0
72	266.5
73	199.0
74	163.5
75	113.0
76	98.0
77	78.0
78	58.0
79	54.0
80	50.0
81	36.0
82	22.0
83	18.5
84	15.0
85	12.5
86	10.0
87	8.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	4.0
100	7.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.084092818600595
2	0.0
3	0.0
4	0.0
5	1.7150992838817328E-4
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0023582615153373825
20	0.003537392273006074
21	0.002829913818404859
22	0.0023797002563859045
23	0.08500460825738838
24	0.3064882420296656
25	0.0014792731323479945
26	9.730958806959787
27	9.710056034437478
28	9.726628181267987
29	10.26456907165749
30	9.687202336479753
31	8.723552365089754
32	9.576857136303014
33	9.325552213732243
34	9.261707642889744
35	9.09429251404184
36	9.590663685538262
37	9.652149994865422
38	9.54860087560106
39	9.691275697278973
40	9.590041962047854
41	9.890934692663855
42	9.750339428867651
43	9.873697944860844
44	9.898202425879305
45	9.704460523023814
46	9.863107206782875
47	9.904612609452812
48	9.933769297278802
49	10.014529034808584
50	9.952549634437307
51	9.745772977024316
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4664453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	56.174897393726575
#Duplication Level	Relative count
1	100.0
2	13.23010345527081
3	3.83934005003719
4	1.8912705389140578
5	1.119074988166881
6	0.8127662451822301
7	0.6863209141929812
8	0.6126174859693015
9	0.6491311109608493
10++	12.3950233281493
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE