FastQCFastQC Report
Wed 23 Jul 2014
SoilN_1_CGATGT_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SoilN_1_CGATGT_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 22858326
Filtered Sequences 0
Sequence length 51
%GC 38

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC 49567 0.2168444005917144 TruSeq Adapter, Index 2 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGAAG 1197055 1.7740933 5.4495597 5
CACCG 562295 1.2596588 6.693751 31
Produced by FastQC (version 0.10.1)