##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SoilN_1_CGATGT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	22858326	
Filtered Sequences	0	
Sequence length	51	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.255379680909265	34.0	33.0	34.0	31.0	34.0
2	33.405858504249174	34.0	34.0	34.0	31.0	34.0
3	33.41367871820535	34.0	34.0	34.0	31.0	34.0
4	36.65877457518105	37.0	37.0	37.0	35.0	37.0
5	36.60311861857251	37.0	37.0	37.0	35.0	37.0
6	36.60830587506714	37.0	37.0	37.0	35.0	37.0
7	36.567578351975556	37.0	37.0	37.0	35.0	37.0
8	36.56592569377128	37.0	37.0	37.0	35.0	37.0
9	38.44141487001279	39.0	39.0	39.0	37.0	39.0
10	38.43003359038628	39.0	39.0	39.0	37.0	39.0
11	38.43872696539545	39.0	39.0	39.0	37.0	39.0
12	38.429351650685184	39.0	39.0	39.0	37.0	39.0
13	38.40894009473835	39.0	39.0	39.0	37.0	39.0
14	40.06560922265261	41.0	40.0	41.0	38.0	41.0
15	40.03742522527678	41.0	40.0	41.0	38.0	41.0
16	40.02259133061625	41.0	40.0	41.0	38.0	41.0
17	40.01447962549838	41.0	40.0	41.0	38.0	41.0
18	39.99392405200626	41.0	40.0	41.0	38.0	41.0
19	40.00479155822697	41.0	40.0	41.0	38.0	41.0
20	39.967602133244576	41.0	40.0	41.0	38.0	41.0
21	39.95132036352968	41.0	40.0	41.0	38.0	41.0
22	39.92379078852931	41.0	40.0	41.0	38.0	41.0
23	39.88965001199126	41.0	40.0	41.0	38.0	41.0
24	39.85716272486445	41.0	40.0	41.0	38.0	41.0
25	39.82946559603708	41.0	40.0	41.0	38.0	41.0
26	39.71570914685529	41.0	40.0	41.0	38.0	41.0
27	39.64902272371126	41.0	40.0	41.0	38.0	41.0
28	39.592581101520736	41.0	40.0	41.0	38.0	41.0
29	39.28937254635357	41.0	40.0	41.0	37.0	41.0
30	39.309920376496514	41.0	39.0	41.0	37.0	41.0
31	39.581015293945846	41.0	40.0	41.0	38.0	41.0
32	39.609315572802664	41.0	40.0	41.0	38.0	41.0
33	39.63726359489317	41.0	40.0	41.0	38.0	41.0
34	39.7265666785923	41.0	40.0	41.0	38.0	41.0
35	39.770785927193444	41.0	40.0	41.0	38.0	41.0
36	39.76912399446923	41.0	40.0	41.0	38.0	41.0
37	39.74150863015953	41.0	40.0	41.0	38.0	41.0
38	39.70638287335652	41.0	40.0	41.0	38.0	41.0
39	39.698573377595544	41.0	40.0	41.0	38.0	41.0
40	39.66456598790305	41.0	40.0	41.0	38.0	41.0
41	39.632484592266294	41.0	40.0	41.0	38.0	41.0
42	39.609152918722046	41.0	40.0	41.0	37.0	41.0
43	39.57318926154085	41.0	40.0	41.0	37.0	41.0
44	39.53139250004572	41.0	40.0	41.0	37.0	41.0
45	39.47222666261737	41.0	40.0	41.0	37.0	41.0
46	39.44866238236343	41.0	40.0	41.0	37.0	41.0
47	39.398945749570636	41.0	40.0	41.0	37.0	41.0
48	39.36422452807786	41.0	39.0	41.0	37.0	41.0
49	39.32299263734361	41.0	39.0	41.0	37.0	41.0
50	39.26310124372187	41.0	39.0	41.0	36.0	41.0
51	38.35295638009538	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	12.0
11	17.0
12	14.0
13	21.0
14	14.0
15	25.0
16	45.0
17	84.0
18	205.0
19	516.0
20	1456.0
21	2453.0
22	3773.0
23	6315.0
24	10865.0
25	16305.0
26	22874.0
27	33065.0
28	45723.0
29	61866.0
30	82500.0
31	108557.0
32	143275.0
33	190264.0
34	261020.0
35	375112.0
36	589113.0
37	1113020.0
38	3092781.0
39	1.667468E7
40	22350.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.191713784144115	31.31489636212063	32.44241570445733	21.05097414927792
2	18.540631540559882	32.07980321918586	32.948873858916876	16.430691381337372
3	16.55564803826842	31.692233280774808	33.442737670291336	18.309381010665433
4	17.167425121157166	30.357008645339995	33.24226804710021	19.233298186402624
5	19.07257464395668	30.494440666096374	32.557287470983205	17.875697218963744
6	19.037496446590183	30.725640189049713	31.067826226644947	19.169037137715158
7	19.570475108282206	30.97820461568358	31.257372040279762	18.193948235754448
8	18.679963703378803	31.11311388244266	31.349089167771954	18.85783324640658
9	18.652686990289666	30.139547401677618	32.090604535082754	19.117161072949962
10	18.755695408316427	30.75964092908641	31.203999802960197	19.280663859636967
11	18.979237587214392	30.510606944708023	31.328225872708266	19.181929595369322
12	18.635546627517694	30.652992699465393	31.238980492272265	19.472480180744643
13	18.56544963091348	31.089525103456833	31.23681497936463	19.108210286265056
14	18.708443479194408	30.94108028733163	31.180695384255174	19.169780849218792
15	18.602725143047458	31.0359775736829	31.2371996843338	19.124097598935844
16	18.58295309989017	30.730898666857758	31.297773074021258	19.38837515923082
17	19.05863056578621	30.67178702053117	31.18003530968773	19.089547103994885
18	18.76599362525497	30.550776115451324	31.363534670036643	19.319695589257062
19	18.775357209136203	30.57094751232156	31.1344463957272	19.519248882815035
20	18.93779547958722	30.556608667584968	31.265540936814823	19.24005491601299
21	19.086408198062387	30.48960470496641	31.0359281209214	19.388058976049802
22	18.834602434099352	30.739143412651966	31.022655173429044	19.403598979819638
23	18.99817993994847	30.750042096790725	30.96272002709594	19.28905793616486
24	18.926687664722696	30.467354182815736	30.941079181781056	19.66487897068051
25	18.89981430071315	30.49318172959109	31.161151430950223	19.445852538745537
26	19.066505076143134	30.53636056135991	30.866407643557036	19.530726718939924
27	18.99920994523046	30.45148739634911	30.849812515273374	19.699490143147063
28	18.950840441720686	30.703498303811948	30.868936155636767	19.4767250988306
29	19.360958172218755	30.497834294779302	30.796055401185384	19.34515213181656
30	19.03491764285436	30.462477997728715	30.99243765676413	19.510166702652796
31	18.983669809502622	30.4912794888336	30.786745881795508	19.738304819868272
32	19.163487581428303	30.747505620606358	30.750793574628588	19.33821322333675
33	19.044301049076267	30.48658558000708	30.73674292088543	19.732370450031222
34	18.997780111411824	30.507839875758343	30.761290792279457	19.733089220550383
35	19.38300050284844	30.554080006537816	30.706386997653578	19.356532492960167
36	19.079956926445472	30.694147571117963	30.738406917322315	19.487488585114246
37	19.0260768928027	30.508856611177542	30.97818519570994	19.48688130030982
38	19.400769998319547	30.535264726899275	30.72183831487803	19.34212695990315
39	19.08994418711688	30.474420444219273	30.94447893465076	19.491156434013092
40	19.034727016789546	30.75590083100842	30.717317250102944	19.492054902099085
41	19.16407014694384	30.540917664983102	30.917905637929998	19.377106550143058
42	19.065555943724753	30.497561206103185	30.711727058088513	19.72515579208355
43	19.01923103511461	30.53780008677102	30.96384528995926	19.479123588155108
44	19.160929984882678	30.563891341534543	30.6867808506452	19.58839782293758
45	19.30694464070642	30.475549452111906	30.721741447219042	19.49576445996263
46	19.03296155927206	30.52797949048422	30.94937457125628	19.489684378987445
47	19.160523389089416	30.80681238120681	30.707430330057722	19.32523389964605
48	19.07157194756065	30.502578626517035	30.941614981401354	19.48423444452096
49	19.258008097585517	30.54824725309252	30.70553158805761	19.488213061264357
50	19.16343315294095	30.566258329307168	30.689049657432715	19.581258860319164
51	19.082921739650967	30.492467959229746	30.67882556550856	19.745784735610723
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	36.24268793342203
2	34.971322921897254
3	34.865029048933856
4	36.40072330755979
5	36.94827186292042
6	38.20653358430534
7	37.76442334403665
8	37.53779694978539
9	37.76984806323963
10	38.03635926795339
11	38.16116718258371
12	38.10802680826234
13	37.67365991717854
14	37.878224328413204
15	37.7268227419833
16	37.97132825912099
17	38.1481776697811
18	38.08568921451204
19	38.29460609195124
20	38.17785039560021
21	38.474467174112185
22	38.238201413918986
23	38.28723787611333
24	38.591566635403204
25	38.34566683945869
26	38.59723179508306
27	38.69870008837752
28	38.427565540551285
29	38.70611030403531
30	38.545084345507156
31	38.72197462937089
32	38.50170080476506
33	38.77667149910749
34	38.730869331962204
35	38.73953299580861
36	38.56744551155972
37	38.51295819311252
38	38.7428969582227
39	38.58110062112997
40	38.52678191888863
41	38.54117669708689
42	38.7907117358083
43	38.49835462326972
44	38.74932780782026
45	38.802709100669055
46	38.5226459382595
47	38.48575728873547
48	38.55580639208161
49	38.74622115884987
50	38.74469201326012
51	38.82870647526169
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2624.0
1	3698.5
2	4773.0
3	5206.5
4	5640.0
5	5955.0
6	6270.0
7	7352.0
8	8434.0
9	10674.0
10	12914.0
11	17268.5
12	21623.0
13	31624.5
14	41626.0
15	55520.5
16	69415.0
17	90478.5
18	111542.0
19	143863.5
20	176185.0
21	222217.0
22	268249.0
23	342354.0
24	416459.0
25	517761.0
26	750204.0
27	881345.0
28	1040154.5
29	1198964.0
30	1370432.5
31	1541901.0
32	1700438.0
33	1858975.0
34	1987651.0
35	2116327.0
36	2170460.0
37	2224593.0
38	2206231.5
39	2187870.0
40	2106789.0
41	2025708.0
42	1900680.0
43	1775652.0
44	1631078.0
45	1486504.0
46	1327742.0
47	1168980.0
48	1029678.5
49	890377.0
50	749566.5
51	608756.0
52	536729.5
53	464703.0
54	365346.0
55	265989.0
56	217176.0
57	168363.0
58	131494.0
59	94625.0
60	74064.0
61	53503.0
62	43762.0
63	34021.0
64	27533.5
65	21046.0
66	16792.0
67	12538.0
68	9073.5
69	5609.0
70	4001.0
71	2393.0
72	1878.5
73	1364.0
74	1163.0
75	865.5
76	769.0
77	678.5
78	588.0
79	508.0
80	428.0
81	359.0
82	290.0
83	240.5
84	191.0
85	143.5
86	96.0
87	72.0
88	48.0
89	36.0
90	24.0
91	15.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04657821399519808
2	0.0
3	0.0
4	0.0
5	5.2497282609408934E-5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.3124320652352233E-5
16	0.0
17	4.3747735507840776E-5
18	0.0
19	0.0018724030797355852
20	6.474664855160435E-4
21	0.0019117760416926418
22	6.562160326176116E-5
23	0.0012643095561765984
24	0.004252279891362123
25	0.0017630337409659832
26	0.11430845810843715
27	0.10703320969348323
28	0.12514039742017855
29	0.747198198153268
30	0.11136423550875947
31	0.006916516983789626
32	0.07600731567132256
33	0.0013649293478446322
34	0.009729496376943788
35	0.002379876811626538
36	0.019231504529246806
37	0.024848713768453563
38	0.0060940595562422204
39	0.006982138587051388
40	0.017695959012921595
41	0.017569090579948855
42	0.007616480751915079
43	0.012118122735671895
44	0.03376450226495151
45	0.002432374094235947
46	0.016466647645151267
47	0.026734241168841495
48	0.010324465579850424
49	0.02660737273586876
50	0.025089326313746687
51	0.0240131320202538
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.2858326E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	58.651659682793934
#Duplication Level	Relative count
1	100.0
2	67.2673399741745
3	30.28846153846154
4	10.400525733259546
5	3.160164176351227
6	1.187511529238148
7	0.5314978786201808
8	0.38622947795609663
9	0.2559490868843387
10++	17.107083563918096
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC	49567	0.2168444005917144	TruSeq Adapter, Index 2 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGAAG	1197055	1.7740933	5.4495597	5
CACCG	562295	1.2596588	6.693751	31
>>END_MODULE