##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	SoilF_1_TGACCA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	28589343	
Filtered Sequences	0	
Sequence length	51	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.25233878232179	34.0	33.0	34.0	31.0	34.0
2	33.404000224839024	34.0	34.0	34.0	31.0	34.0
3	33.412315735971966	34.0	34.0	34.0	31.0	34.0
4	36.656185908154654	37.0	37.0	37.0	35.0	37.0
5	36.60148276230063	37.0	37.0	37.0	35.0	37.0
6	36.6067713763132	37.0	37.0	37.0	35.0	37.0
7	36.56639098002357	37.0	37.0	37.0	35.0	37.0
8	36.56529588665259	37.0	37.0	37.0	35.0	37.0
9	38.439599643825325	39.0	39.0	39.0	37.0	39.0
10	38.429093771060074	39.0	39.0	39.0	37.0	39.0
11	38.43736772824755	39.0	39.0	39.0	37.0	39.0
12	38.42887449354817	39.0	39.0	39.0	37.0	39.0
13	38.40773721172956	39.0	39.0	39.0	37.0	39.0
14	40.06242231589582	41.0	40.0	41.0	38.0	41.0
15	40.03504438699413	41.0	40.0	41.0	38.0	41.0
16	40.01754094174182	41.0	40.0	41.0	38.0	41.0
17	40.008905031500724	41.0	40.0	41.0	38.0	41.0
18	39.98951570870307	41.0	40.0	41.0	38.0	41.0
19	39.99913932964461	41.0	40.0	41.0	38.0	41.0
20	39.96236184930867	41.0	40.0	41.0	38.0	41.0
21	39.94661710134437	41.0	40.0	41.0	38.0	41.0
22	39.917722943126044	41.0	40.0	41.0	38.0	41.0
23	39.88454488093693	41.0	40.0	41.0	38.0	41.0
24	39.85208617770615	41.0	40.0	41.0	38.0	41.0
25	39.824542487737475	41.0	40.0	41.0	38.0	41.0
26	39.712835233744265	41.0	40.0	41.0	38.0	41.0
27	39.645906763229924	41.0	40.0	41.0	38.0	41.0
28	39.589110075037404	41.0	40.0	41.0	38.0	41.0
29	39.28627975116462	41.0	40.0	41.0	37.0	41.0
30	39.30773180062235	40.0	39.0	41.0	37.0	41.0
31	39.57883852035354	41.0	40.0	41.0	38.0	41.0
32	39.606737762389294	41.0	40.0	41.0	38.0	41.0
33	39.635182382470276	41.0	40.0	41.0	38.0	41.0
34	39.72760734655567	41.0	40.0	41.0	38.0	41.0
35	39.77380718402658	41.0	40.0	41.0	38.0	41.0
36	39.77538011279238	41.0	40.0	41.0	38.0	41.0
37	39.74531331482504	41.0	40.0	41.0	38.0	41.0
38	39.709028325694646	41.0	40.0	41.0	38.0	41.0
39	39.702494002747805	41.0	40.0	41.0	38.0	41.0
40	39.66551886134634	41.0	40.0	41.0	38.0	41.0
41	39.63482801965753	41.0	40.0	41.0	38.0	41.0
42	39.61154028618286	41.0	40.0	41.0	37.0	41.0
43	39.57580301862831	41.0	40.0	41.0	37.0	41.0
44	39.53476496469331	41.0	40.0	41.0	37.0	41.0
45	39.4749342788325	41.0	40.0	41.0	37.0	41.0
46	39.452106227135054	41.0	40.0	41.0	37.0	41.0
47	39.401868031734764	41.0	40.0	41.0	37.0	41.0
48	39.36478162509716	41.0	39.0	41.0	37.0	41.0
49	39.32252493525297	41.0	39.0	41.0	36.0	41.0
50	39.26087196197548	41.0	39.0	41.0	36.0	41.0
51	38.349910279505195	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	6.0
11	3.0
12	7.0
13	8.0
14	12.0
15	11.0
16	33.0
17	99.0
18	212.0
19	555.0
20	1669.0
21	2825.0
22	4449.0
23	7616.0
24	13154.0
25	19511.0
26	27739.0
27	40618.0
28	56291.0
29	77034.0
30	103118.0
31	136462.0
32	178813.0
33	238562.0
34	328408.0
35	470215.0
36	739158.0
37	1400901.0
38	3894568.0
39	2.0819867E7
40	27416.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.855665000392987	31.43650087706657	31.401866843356114	21.305967279184326
2	18.877159926340383	31.55478948921631	31.931961500479396	17.63608908396391
3	17.05621916530226	30.613309302001102	32.90632107215615	19.42415046054049
4	17.645792000186923	29.89162779991132	32.8073296402789	19.655250559622864
5	19.362179147151945	30.280241431474764	32.17737691003627	18.180202511337015
6	19.227314877435273	30.418715813091612	30.86977899422173	19.484190315251386
7	19.690725316772756	30.75633812221568	31.045187012517218	18.507749548494346
8	18.885418248331202	30.820620816644862	31.151705724752055	19.142255210271884
9	18.883823248404134	29.931544771770373	31.77400753840338	19.410624441422105
10	19.02586918489173	30.51467114861646	30.905704968456256	19.553754698035558
11	19.24452758498158	30.227459231924286	31.025987550675787	19.502025632418345
12	18.932886985195847	30.34436293271937	30.919349913007792	19.803400169076987
13	18.88916789728256	30.779703472024522	30.86917737144222	19.461951259250693
14	19.00885235452945	30.637440671511758	30.83059306399591	19.523113909962884
15	18.91373715421642	30.68701965928422	30.859877782426754	19.53936540407261
16	18.912442304113107	30.473327071559495	30.89399431109697	19.720236313230423
17	19.32714972144155	30.39852767458855	30.826344595949283	19.447978008020613
18	19.04825165097358	30.27375620349163	30.978123561636234	19.699868583898553
19	19.082239859368592	30.322000133129162	30.74501927801948	19.85074072948276
20	19.235530453863742	30.315702845252623	30.862928292573844	19.58583840830979
21	19.351746248370077	30.2067552809365	30.661297333002107	19.780201137691318
22	19.140982896903548	30.440808573101723	30.652975169823872	19.765233360170857
23	19.297709706850927	30.477445104418464	30.583657269067505	19.641187919663096
24	19.208058244246054	30.202132959714827	30.564506715594142	20.025302080444973
25	19.206700009027994	30.23821134227227	30.75230674664608	19.802781902053656
26	19.363192918818882	30.26757184470752	30.52501152798625	19.844223708487345
27	19.303166936302752	30.13885391725854	30.504236110520228	20.05374303591848
28	19.264568606766076	30.394699728096896	30.512170218810592	19.828561446326436
29	19.6378248458891	30.23754163466485	30.417306262469605	19.70732725697644
30	19.32978200086114	30.154578418928462	30.615738524293267	19.899901055917134
31	19.306674591816815	30.229029353453473	30.419259542056025	20.04503651267368
32	19.45890570613295	30.46959921626175	30.380470588095278	19.691024489510024
33	19.350287322599698	30.192660134887806	30.379165792931502	20.077886749581
34	19.310351100123047	30.24349016977375	30.578402624308787	19.867756105794417
35	19.6656275505034	30.265640793489208	30.353871399414572	19.71486025659282
36	19.37572094659562	30.392963911073444	30.34059185945902	19.89072328287191
37	19.348265867742068	30.260021351584548	30.34813144946219	20.04358133121119
38	19.48970301376947	30.269062923391786	30.339803413985116	19.901430648853623
39	19.36807835478596	30.382841674506654	30.35175805568921	19.89732191501817
40	19.361704658437894	30.460862026863733	30.353813602311202	19.823619712387167
41	19.495846726691394	30.265736864830178	30.51505430821424	19.723362100264193
42	19.395398677195864	30.187522774535445	30.343892905454766	20.07318564281392
43	19.359329746701956	30.276031573391826	30.51958462559027	19.84505405431595
44	19.481492014701868	30.285371065941323	30.32042732881144	19.91270959054537
45	19.578604715458823	30.185788143088182	30.346470555560916	19.88913658589207
46	19.379572386958944	30.26050341704703	30.51569176577918	19.84423243021485
47	19.476450059595457	30.479604261119732	30.335593425162383	19.70835225412243
48	19.39914758829026	30.19412608524866	30.5121686971587	19.894557629302387
49	19.57152479191476	30.249589623450017	30.31185678852924	19.86702879610598
50	19.495059568257005	30.280909374331866	30.3025940314853	19.92143702592583
51	19.40018956945371	30.18170384677929	30.331235045130857	20.086871538636146
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.16163227957732
2	36.51324901030429
3	36.48036962584275
4	37.30104255980979
5	37.542381658488964
6	38.71150519268666
7	38.1984748652671
8	38.02767345860309
9	38.29444768982624
10	38.57962388292728
11	38.74655321739993
12	38.73628715427284
13	38.35111915653326
14	38.53196626449233
15	38.45310255828903
16	38.63267861734353
17	38.77512772946217
18	38.74812023487213
19	38.93298058885135
20	38.82136886217353
21	39.13194738606139
22	38.906216257074405
23	38.93889762651403
24	39.23336032469103
25	39.00948191108165
26	39.20741662730623
27	39.35690997222123
28	39.093130053092516
29	39.34515210286554
30	39.22968305677827
31	39.35171110449049
32	39.14993019564297
33	39.4281740721807
34	39.17810720591746
35	39.38048780709622
36	39.26644422946753
37	39.391847198953265
38	39.3911336626231
39	39.26540026980413
40	39.185324370825064
41	39.21920882695559
42	39.46858432000978
43	39.204383801017904
44	39.39420160524724
45	39.4677413013509
46	39.22380481717379
47	39.18480231371789
48	39.293705217592645
49	39.438553588020746
50	39.41649659418284
51	39.487061108089854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13368.0
1	16631.5
2	19895.0
3	19532.5
4	19170.0
5	17464.0
6	15758.0
7	15983.0
8	16208.0
9	15775.0
10	15342.0
11	17142.0
12	18942.0
13	22722.0
14	26502.0
15	35615.5
16	44729.0
17	60739.0
18	76749.0
19	107161.0
20	137573.0
21	187087.5
22	236602.0
23	316490.5
24	396379.0
25	519589.0
26	806893.0
27	970987.0
28	1171008.0
29	1371029.0
30	1594380.0
31	1817731.0
32	2032572.0
33	2247413.0
34	2428178.0
35	2608943.0
36	2712198.0
37	2815453.0
38	2833015.0
39	2850577.0
40	2765374.5
41	2680172.0
42	2527301.5
43	2374431.0
44	2186934.0
45	1999437.0
46	1787149.5
47	1574862.0
48	1391412.0
49	1207962.0
50	1022161.5
51	836361.0
52	728210.0
53	620059.0
54	495953.0
55	371847.0
56	302371.0
57	232895.0
58	182242.0
59	131589.0
60	104177.0
61	76765.0
62	63993.5
63	51222.0
64	41404.5
65	31587.0
66	25042.5
67	18498.0
68	13605.5
69	8713.0
70	6326.0
71	3939.0
72	3062.5
73	2186.0
74	1809.0
75	1290.5
76	1149.0
77	944.5
78	740.0
79	647.5
80	555.0
81	460.5
82	366.0
83	284.0
84	202.0
85	169.0
86	136.0
87	93.0
88	50.0
89	37.0
90	24.0
91	17.5
92	11.0
93	6.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04688810092627872
2	0.0
3	0.0
4	0.0
5	1.049342057283373E-4
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	3.4978068576112435E-6
16	0.0
17	3.847587543372368E-5
18	0.0
19	0.0019797586814079638
20	7.415350538135837E-4
21	0.0019902521019807976
22	5.9462716579391135E-5
23	0.0011857565247302116
24	0.004127412091981268
25	0.0016999341327990642
26	0.11455667239362584
27	0.1066131530199907
28	0.12518300962704879
29	0.7462535952645012
30	0.11122676026517993
31	0.006547894437448248
32	0.07569953601242253
33	0.0013921271293292749
34	0.009681929381867922
35	0.002182631479149416
36	0.018870667996812657
37	0.024208321261527416
38	0.006138651035107733
39	0.006778749690050589
40	0.01777935225723795
41	0.017142751409152703
42	0.007142521603242159
43	0.011801600337580336
44	0.03347750943419721
45	0.0022770722643049193
46	0.015998968566713827
47	0.026114625998925545
48	0.010220591637940053
49	0.02681418737044779
50	0.024211819068385026
51	0.023361852001985494
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.8589343E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	68.28102175751519
#Duplication Level	Relative count
1	100.0
2	89.55325627546924
3	51.868627955946735
4	22.65228522518974
5	8.162275984305646
6	2.970717001711836
7	1.2891790762145703
8	0.7922921937252883
9	0.5953553195679508
10++	25.095817363772703
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC	52566	0.18386571527719262	TruSeq Adapter, Index 4 (100% over 51bp)
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE