##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename SoilF2Pool_1_CAGATC_L005_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17445990 Filtered Sequences 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.218055897085804 34.0 33.0 34.0 31.0 34.0 2 33.37374153028862 34.0 34.0 34.0 31.0 34.0 3 33.380628614369265 34.0 34.0 34.0 31.0 34.0 4 36.63606398948985 37.0 37.0 37.0 35.0 37.0 5 36.5763793284302 37.0 37.0 37.0 35.0 37.0 6 36.580369242444824 37.0 37.0 37.0 35.0 37.0 7 36.54099262925177 37.0 37.0 37.0 35.0 37.0 8 36.53888062528982 37.0 37.0 37.0 35.0 37.0 9 38.403799268485194 39.0 39.0 39.0 37.0 39.0 10 38.39436569664433 39.0 39.0 39.0 37.0 39.0 11 38.40017356424026 39.0 39.0 39.0 37.0 39.0 12 38.39183577429541 39.0 39.0 39.0 37.0 39.0 13 38.369394972712925 39.0 39.0 39.0 37.0 39.0 14 40.01054259460197 41.0 40.0 41.0 38.0 41.0 15 39.982022115110695 41.0 40.0 41.0 38.0 41.0 16 39.963791450069614 41.0 40.0 41.0 38.0 41.0 17 39.95351934742597 41.0 40.0 41.0 38.0 41.0 18 39.93438612540761 41.0 40.0 41.0 38.0 41.0 19 39.942050637424416 41.0 40.0 41.0 38.0 41.0 20 39.9022366171252 41.0 40.0 41.0 38.0 41.0 21 39.88499884500679 41.0 40.0 41.0 38.0 41.0 22 39.85554852433138 41.0 40.0 41.0 38.0 41.0 23 39.81806908063113 41.0 40.0 41.0 38.0 41.0 24 39.78168943121027 41.0 40.0 41.0 38.0 41.0 25 39.753640750682536 41.0 40.0 41.0 38.0 41.0 26 39.63643307143934 41.0 40.0 41.0 38.0 41.0 27 39.5668573121961 41.0 40.0 41.0 37.0 41.0 28 39.50819770044578 41.0 40.0 41.0 37.0 41.0 29 39.200932535212964 41.0 39.0 41.0 37.0 41.0 30 39.22142767478372 40.0 39.0 41.0 37.0 41.0 31 39.495009340255265 41.0 40.0 41.0 37.0 41.0 32 39.51991770028528 41.0 40.0 41.0 37.0 41.0 33 39.54567857714007 41.0 40.0 41.0 37.0 41.0 34 39.64089346606298 41.0 40.0 41.0 38.0 41.0 35 39.68698388569522 41.0 40.0 41.0 38.0 41.0 36 39.68775965135828 41.0 40.0 41.0 38.0 41.0 37 39.65581460266801 41.0 40.0 41.0 38.0 41.0 38 39.61790743890143 41.0 40.0 41.0 38.0 41.0 39 39.60745604004129 41.0 40.0 41.0 37.0 41.0 40 39.569736426536984 41.0 40.0 41.0 37.0 41.0 41 39.53330639304505 41.0 40.0 41.0 37.0 41.0 42 39.50634667336161 41.0 40.0 41.0 37.0 41.0 43 39.467125052805834 41.0 40.0 41.0 37.0 41.0 44 39.42004196952996 41.0 40.0 41.0 37.0 41.0 45 39.35684842190096 41.0 39.0 41.0 37.0 41.0 46 39.33318934609042 41.0 39.0 41.0 37.0 41.0 47 39.278047562792366 41.0 39.0 41.0 36.0 41.0 48 39.234913868459174 41.0 39.0 41.0 36.0 41.0 49 39.188495981024865 40.0 39.0 41.0 36.0 41.0 50 39.1193236955885 40.0 39.0 41.0 36.0 41.0 51 38.19045740597123 40.0 38.0 41.0 34.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 8.0 10 26.0 11 30.0 12 32.0 13 21.0 14 32.0 15 35.0 16 67.0 17 95.0 18 196.0 19 406.0 20 1185.0 21 2021.0 22 3215.0 23 5482.0 24 9314.0 25 13752.0 26 19374.0 27 27657.0 28 38407.0 29 51895.0 30 68985.0 31 91088.0 32 118159.0 33 158452.0 34 216761.0 35 310421.0 36 490331.0 37 931582.0 38 2562151.0 39 1.2310708E7 40 14101.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.387669758437895 31.15146509652913 31.61774543372547 21.843119711307505 2 19.225013885712418 31.572005945205746 32.577193956892096 16.625786212189738 3 17.146106354526168 31.101473748408658 33.004593032553615 18.74782686451156 4 17.925185099842427 29.861051164193032 32.748562850259574 19.465200885704967 5 19.686508833153407 30.10899704218359 31.926980533851413 18.27751359081159 6 19.572062118572806 30.286312212720517 30.644990625352875 19.496635043353802 7 20.24008955639663 30.450453084061152 30.69452063196184 18.61493672758038 8 19.301432592819324 30.518720920968086 30.888846090133033 19.291000396079557 9 19.154252639145156 29.776005832859013 31.551600109824662 19.51814141817117 10 19.405169898641464 30.218995883867866 30.659589968812316 19.71624424867835 11 19.445505815376485 30.1532099926688 30.78792891661637 19.613355275338346 12 19.294095663244104 30.306677924267984 30.6663021129784 19.732924299509516 13 19.196709387085516 30.548487073533803 30.65890213166464 19.595901407716042 14 19.322457481633315 30.59065722266263 30.640812014680733 19.446073281023317 15 19.247950832475112 30.511257402633625 30.668548589426326 19.572243175464937 16 19.212546837410773 30.41341878563498 30.695512263849743 19.678522113104503 17 19.48752566922844 30.335264512994563 30.636789459764557 19.54042035801244 18 19.380138358442256 30.241511086501827 30.638083593994953 19.740266961060964 19 19.37955742254241 30.23633666730121 30.571928853722767 19.81217705643361 20 19.524790161214447 30.273463215669157 30.55238272984937 19.649363893267026 21 19.508447730641116 30.189442262682142 30.510084128795107 19.79202587788164 22 19.422787270669907 30.225747746887432 30.493706371388658 19.857758611054 23 19.597416774161598 30.27259766600343 30.439996276464278 19.6899892833707 24 19.533518120819604 30.14191529907972 30.419274227132693 19.905292352967983 25 19.476843431002617 30.177345950939362 30.462167244192774 19.883643373865247 26 19.652899166446357 30.191745972867455 30.377431542433754 19.77792331825243 27 19.62735245104998 30.092638868503048 30.34324029701267 19.9367683834343 28 19.541747449341216 30.198018608701098 30.33295642722856 19.927277514729123 29 19.755338439751963 30.183216942093754 30.279355722902473 19.78208889525181 30 19.648400308856488 30.110213748153424 30.3160511265825 19.925334816407588 31 19.560761857287098 30.16770802509991 30.270976372270958 20.000553745342035 32 19.727885810361013 30.28242523915468 30.250359165834663 19.73932978464964 33 19.65633649682031 30.143442566569878 30.242733349963956 19.957487586645854 34 19.598513598471293 30.166214397994253 30.264464385654964 19.97080761787949 35 19.75293688952671 30.28502400865594 30.198056436405707 19.76398266541164 36 19.715427578133152 30.16597239182556 30.228738047981214 19.889861982060076 37 19.625814953251954 30.250728737943188 30.21174766500564 19.911708643799223 38 19.7356392182184 30.24657905199878 30.242319920719396 19.775461809063433 39 19.653336648941576 30.157165219154887 30.239143976905847 19.950354154997694 40 19.619700329234696 30.23677402740236 30.23203856978631 19.911487073576627 41 19.74610430895162 30.2432495804905 30.256097148482 19.754548962075884 42 19.673427141095086 30.1635403750243 30.232678919989546 19.930353563891074 43 19.62657974413383 30.222613827137707 30.243979480488438 19.906826948240028 44 19.710986327976613 30.25815682411677 30.2220506137167 19.808806234189916 45 19.725448767480092 30.162958368776927 30.239344566755094 19.872248296987888 46 19.617933154153448 30.19418823794609 30.264410533447517 19.923468074452945 47 19.719140001708578 30.320719115347767 30.219133408212517 19.74100747473114 48 19.667319739624585 30.18407783402125 30.25075903465204 19.89784339170212 49 19.647101979914865 30.221933432240018 30.209726812525357 19.921237775319764 50 19.74533219292475 30.243103568676954 30.190413756639117 19.82115048175918 51 19.68442771053771 30.15812097065807 30.21610204255855 19.941349276245663 >>END_MODULE >>Per base GC content pass #Base %GC 1 37.230789469745396 2 35.85080009790215 3 35.89393321903773 4 37.3903859855474 5 37.96402242396499 6 39.06869716192661 7 38.855026283977004 8 38.592432988898885 9 38.672394057316325 10 39.12141414731982 11 39.05886109071483 12 39.02701996275362 13 38.792610794801554 14 38.76853076265663 15 38.82019400794005 16 38.891068950515276 17 39.02794602724088 18 39.12040531950322 19 39.191734478976024 20 39.17415405448148 21 39.30047360852275 22 39.2805458817239 23 39.2874060575323 24 39.43881047378759 25 39.36048680486787 26 39.43082248469879 27 39.56412083448428 28 39.469024964070336 29 39.53742733500377 30 39.57373512526408 31 39.56131560262913 32 39.46721559501066 33 39.61382408346616 34 39.56932121635079 35 39.51691955493835 36 39.60528956019323 37 39.53752359705118 38 39.51110102728183 39 39.603690803939266 40 39.53118740281133 41 39.5006532710275 42 39.60378070498616 43 39.53340669237386 44 39.51979256216653 45 39.59769706446798 46 39.5414012286064 47 39.460147476439715 48 39.565163131326706 49 39.568339755234625 50 39.566482674683925 51 39.62577698678338 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1508.0 1 1991.0 2 2474.0 3 2459.0 4 2444.0 5 2328.5 6 2213.0 7 2463.5 8 2714.0 9 3213.0 10 3712.0 11 5530.0 12 7348.0 13 11114.5 14 14881.0 15 22344.5 16 29808.0 17 41868.0 18 53928.0 19 73402.5 20 92877.0 21 123174.5 22 153472.0 23 202717.5 24 251963.0 25 326369.0 26 496560.5 27 592346.0 28 708801.5 29 825257.0 30 957522.0 31 1089787.0 32 1216579.0 33 1343371.0 34 1450153.0 35 1556935.0 36 1618673.5 37 1680412.0 38 1693333.5 39 1706255.0 40 1658968.0 41 1611681.0 42 1528130.5 43 1444580.0 44 1337659.0 45 1230738.0 46 1109457.5 47 988177.0 48 880838.0 49 773499.0 50 657999.5 51 542500.0 52 463143.0 53 383786.0 54 316549.5 55 249313.0 56 205162.5 57 161012.0 58 127112.5 59 93213.0 60 74832.5 61 56452.0 62 48510.0 63 40568.0 64 33337.5 65 26107.0 66 20585.5 67 15064.0 68 11181.0 69 7298.0 70 5290.0 71 3282.0 72 2493.0 73 1704.0 74 1324.5 75 798.0 76 651.0 77 525.5 78 400.0 79 320.0 80 240.0 81 192.5 82 145.0 83 118.0 84 91.0 85 64.0 86 37.0 87 26.5 88 16.0 89 12.0 90 8.0 91 5.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046985009162564005 2 0.0 3 0.0 4 0.0 5 6.87837147676916E-5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.71959286919229E-5 16 0.0 17 2.2927904922563867E-5 18 0.0 19 0.0023558422307934372 20 5.560016943721738E-4 21 0.002052047490569466 22 7.451569099833257E-5 23 0.0011807871035120392 24 0.0041556827672147014 25 0.0017998405364212636 26 0.11324665438877358 27 0.10498687664041995 28 0.12420046096552848 29 0.7474325045468901 30 0.10962977738723913 31 0.0065803087127758305 32 0.07586270541253319 33 0.0015017777724279332 34 0.009818875283087976 35 0.0023329143258708734 36 0.01908748084803442 37 0.02402844435884693 38 0.006408349425856601 39 0.007033134834996467 40 0.01784937398221597 41 0.0171959286919229 42 0.007491692933447744 43 0.012065809965499236 44 0.03311362668441287 45 0.0024647497791756155 46 0.0161183171605624 47 0.026137811611722808 48 0.010277433381539254 49 0.026561977852790238 50 0.024578714076988466 51 0.023449514759552193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1.744599E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Duplicate Percentage 50.59872587347715 #Duplication Level Relative count 1 100.0 2 48.69222809841343 3 17.04319000536522 4 5.257913696635242 5 1.692917912163716 6 0.7175979152295547 7 0.4071817276002146 8 0.29221276921897754 9 0.22131524488388135 10++ 17.290373265884877 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content pass >>END_MODULE