FastQCFastQC Report
Wed 23 Jul 2014
DGY955_1_GCCAAT_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename DGY955_1_GCCAAT_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 24478116
Filtered Sequences 0
Sequence length 51
%GC 38

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC 108341 0.44260350755752603 TruSeq Adapter, Index 6 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GAAGA 2671980 2.2401228 6.691985 6
GGAAG 1404935 1.8715601 9.035239 5
AAGAG 1967710 1.6496805 6.08366 7
GCCAA 1241215 1.5956583 8.329102 34
CTCCA 1249265 1.5567942 8.041578 24
CGCCA 708195 1.4211087 11.938006 33
TCCAG 1106790 1.4040067 8.088461 25
AGAGC 1019565 1.3342437 8.265487 8
CTGAA 1612105 1.3101339 5.5650573 19
CCAAT 1623900 1.2964457 5.4646635 35
CCAGT 1003320 1.272751 7.901128 26
GAGCA 957975 1.2536445 8.248592 9
TGAAC 1520470 1.2356634 5.5033445 20
CGGAA 942640 1.2335765 8.16842 4
ATGCC 964035 1.2229164 7.858192 47
AGCAC 915855 1.1773881 7.9971986 10
CACAC 907630 1.146237 7.8626866 12
ACTCC 896515 1.1172084 7.6244917 23
TCTGA 1382815 1.1089131 5.300598 18
GAACT 1326230 1.0778074 5.3414025 21
ACGCC 507790 1.0189635 11.500196 32
ATCTC 1267390 0.99842715 5.0736732 40
GCACA 774700 0.9959246 7.8333316 11
AACTC 1213360 0.96868986 5.1269855 22
GTCTG 758530 0.9665293 7.660772 17
CACGC 477065 0.9573087 11.455292 31
GTCAC 748330 0.9492861 7.6147475 29
TCTCG 756370 0.9467803 7.430053 41
CGTCT 755125 0.9452219 7.485845 16
ATCGG 706715 0.9125898 7.8744407 2
TATGC 1130665 0.9067078 5.0195575 46
CTCGT 723820 0.9060361 7.3793135 42
ACGTC 706015 0.8956078 7.582365 15
CAGTC 699700 0.887597 7.548313 27
TCGGA 679875 0.87793094 7.8671656 3
AGTCA 1059410 0.86096686 5.104779 28
GTATG 1052230 0.858957 5.0951724 45
GATCG 646555 0.83490443 7.639897 1
CACGT 625150 0.7930274 7.4666696 14
TCACG 617680 0.7835514 7.4009476 30
ACACG 560065 0.71999806 7.5318565 13
Produced by FastQC (version 0.10.1)