##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	DGY955_1_GCCAAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	24478116	
Filtered Sequences	0	
Sequence length	51	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.21799639318647	34.0	33.0	34.0	31.0	34.0
2	33.37087241518097	34.0	34.0	34.0	31.0	34.0
3	33.377744676101706	34.0	34.0	34.0	31.0	34.0
4	36.63690616549084	37.0	37.0	37.0	35.0	37.0
5	36.579958318687595	37.0	37.0	37.0	35.0	37.0
6	36.58307620570145	37.0	37.0	37.0	35.0	37.0
7	36.54198676891637	37.0	37.0	37.0	35.0	37.0
8	36.54149486014365	37.0	37.0	37.0	35.0	37.0
9	38.40823125439883	39.0	39.0	39.0	37.0	39.0
10	38.39636502253686	39.0	39.0	39.0	37.0	39.0
11	38.40505016807666	39.0	39.0	39.0	37.0	39.0
12	38.39632465995341	39.0	39.0	39.0	37.0	39.0
13	38.37616865611716	39.0	39.0	39.0	37.0	39.0
14	40.021009419188964	41.0	40.0	41.0	38.0	41.0
15	39.99256078368123	41.0	40.0	41.0	38.0	41.0
16	39.976731583427416	41.0	40.0	41.0	38.0	41.0
17	39.965372580144646	41.0	40.0	41.0	38.0	41.0
18	39.939548125354094	41.0	40.0	41.0	38.0	41.0
19	39.95173958649432	41.0	40.0	41.0	38.0	41.0
20	39.91467321259528	41.0	40.0	41.0	38.0	41.0
21	39.89808884801428	41.0	40.0	41.0	38.0	41.0
22	39.87087776690004	41.0	40.0	41.0	38.0	41.0
23	39.83432491291405	41.0	40.0	41.0	38.0	41.0
24	39.799453560886796	41.0	40.0	41.0	38.0	41.0
25	39.77022855843971	41.0	40.0	41.0	38.0	41.0
26	39.65400266098911	41.0	40.0	41.0	38.0	41.0
27	39.586005720374885	41.0	40.0	41.0	37.0	41.0
28	39.524599564770426	41.0	40.0	41.0	37.0	41.0
29	39.22186887258807	41.0	39.0	41.0	37.0	41.0
30	39.23687427578168	40.0	39.0	41.0	37.0	41.0
31	39.51047012768466	41.0	40.0	41.0	37.0	41.0
32	39.53431648906313	41.0	40.0	41.0	37.0	41.0
33	39.563701103467274	41.0	40.0	41.0	37.0	41.0
34	39.65466026878866	41.0	40.0	41.0	38.0	41.0
35	39.702601458380215	41.0	40.0	41.0	38.0	41.0
36	39.70252269414852	41.0	40.0	41.0	38.0	41.0
37	39.66913883405079	41.0	40.0	41.0	38.0	41.0
38	39.630374371949216	41.0	40.0	41.0	38.0	41.0
39	39.62500455508913	41.0	40.0	41.0	38.0	41.0
40	39.58517796059141	41.0	40.0	41.0	37.0	41.0
41	39.55342175843925	41.0	40.0	41.0	37.0	41.0
42	39.52805395644011	41.0	40.0	41.0	37.0	41.0
43	39.48994738810781	41.0	40.0	41.0	37.0	41.0
44	39.44663000208023	41.0	40.0	41.0	37.0	41.0
45	39.38342897794912	41.0	40.0	41.0	37.0	41.0
46	39.35331746936733	41.0	39.0	41.0	37.0	41.0
47	39.299946409274305	41.0	39.0	41.0	37.0	41.0
48	39.263103214316004	41.0	39.0	41.0	36.0	41.0
49	39.22111905997994	41.0	39.0	41.0	36.0	41.0
50	39.15565724094125	40.0	39.0	41.0	36.0	41.0
51	38.236653425451536	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	10.0
10	29.0
11	52.0
12	46.0
13	53.0
14	60.0
15	65.0
16	86.0
17	133.0
18	324.0
19	665.0
20	1892.0
21	2959.0
22	4627.0
23	7934.0
24	13259.0
25	19781.0
26	27896.0
27	39361.0
28	54814.0
29	72231.0
30	95924.0
31	125676.0
32	165677.0
33	218953.0
34	300770.0
35	429911.0
36	672131.0
37	1267698.0
38	3497746.0
39	1.7436329E7
40	21022.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.961533989172045	31.23757509405827	31.526366090295856	21.274524826473833
2	18.99886821355042	31.84402345343898	33.28914284089511	15.867965492115488
3	16.422771262298127	31.381071157600527	34.03149163930754	18.16466594079381
4	17.33791930718851	30.35115937844236	32.9016497838314	19.409271530537726
5	19.64529416229684	30.247344502343854	31.922222186816402	18.185139148542905
6	19.51205721878269	30.32407804587575	30.94983290380681	19.214031831534747
7	19.785832373700657	31.089202289914798	30.799241248795457	18.325724087589094
8	18.825827935450587	30.932008819633015	31.206429449063812	19.03573379585259
9	19.115617394737406	29.741549553895407	31.87059821107147	19.272234840295717
10	18.99188646707941	30.669239413686906	30.936645614392873	19.402228504840814
11	19.487806986452718	30.238442370319678	30.984132929184582	19.289617714043025
12	18.88264194842446	30.38187252646405	30.90882484583372	19.82666067927777
13	18.854731303667325	31.038111756640095	30.834068275515975	19.273088664176605
14	18.993880084562065	30.622532387705004	30.82982775308361	19.553759774649322
15	18.895213904143105	31.001886011316067	30.827759034049762	19.27514105049106
16	18.903109209875467	30.4555301559973	30.845699072592026	19.795661561535212
17	19.612872040600525	30.364095133496427	30.77348951969654	19.249543306206508
18	19.075091400008073	30.265127430558792	31.202062282897913	19.457718886535222
19	19.08684778328822	30.301396029555555	30.698935621121095	19.91282056603513
20	19.22176473556093	30.295541557920814	31.0968098016398	19.385883904878455
21	19.627433732151868	30.191895196398082	30.644557447387065	19.53611362406298
22	19.13312464064814	30.678890982461137	30.625157232396077	19.56282714449464
23	19.29169984463092	30.706864162826765	30.574618033133067	19.426817959409252
24	19.18348315420618	30.1958167544146	30.566485377629615	20.054214713749605
25	19.206655296321046	30.204752013546365	30.99713420880746	19.59145848132513
26	19.362718794252913	30.244023321039588	30.489913280650825	19.903344604056674
27	19.28760210405074	30.134451364867594	30.49487200816023	20.083074522921436
28	19.267197228250257	30.620036006503	30.47221906129327	19.640547703953477
29	19.882571080489292	30.22944963039792	30.399524209347888	19.488455079764904
30	19.30703194606531	30.113751737910693	30.90633921521996	19.672877100804033
31	19.3007202369846	30.190206314809288	30.397486538837803	20.11158690936831
32	19.44480388108165	30.69095686993391	30.382885261324883	19.481353987659556
33	19.334020732862022	30.139323620270957	30.41023077316841	20.11642487369861
34	19.771099638968668	30.18308556052397	30.39913236446821	19.64668243603915
35	19.458378403508945	30.251954350532113	30.328587777650633	19.96107946830831
36	19.360080335230258	30.17898949829176	30.36390419379828	20.097025972679695
37	19.32515037845768	30.705919711226944	30.36489447222916	19.60403543808622
38	19.45880952804097	30.715542746039638	30.32900613831295	19.496641587606444
39	19.34029604794795	30.197693097747774	30.817760458218217	19.644250396086054
40	19.335376120506574	30.717153626464793	30.38068703155936	19.56678322146927
41	19.46198293064141	30.242954546160313	30.785003683122962	19.51005884007532
42	19.3688319500788	30.16413323988797	30.382405306284838	20.084629503748396
43	19.30962556274352	30.249634098737243	30.809650363376257	19.631089975142984
44	19.436106550640783	30.256896399672712	30.33767305210613	19.96932399758038
45	19.806248369167093	30.198455946706698	30.374486830223795	19.620808853902417
46	19.348954945401935	30.218004089869922	30.815806798101242	19.617234166626897
47	19.443604580387685	30.74778294618245	30.333420196094625	19.47519227733524
48	19.340736977961278	30.205527520608992	30.80319614597442	19.650539355455308
49	19.802614356970395	30.253818668796818	30.317566330054497	19.62600064417829
50	19.42694215053668	30.286424316190104	30.333906787006264	19.952726746266954
51	19.347326443511452	30.209048344339955	30.346820809248555	20.096804402900037
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.236058815645876
2	34.86683370566591
3	34.58743720309194
4	36.74719083772624
5	37.830433310839744
6	38.726089050317434
7	38.111556461289744
8	37.861561731303176
9	38.38785223503312
10	38.394114971920224
11	38.777424700495736
12	38.70930262770223
13	38.12781996784393
14	38.54763985921139
15	38.17035495463416
16	38.69877077141067
17	38.86241534680703
18	38.532810286543295
19	38.99966834932335
20	38.607648640439386
21	39.16354735621485
22	38.69595178514278
23	38.71851780404017
24	39.23769786795578
25	38.79811377764618
26	39.26606339830958
27	39.37067662697218
28	38.907744932203734
29	39.371026160254196
30	38.97990904686935
31	39.41230714635291
32	38.92615786874121
33	39.450445606560635
34	39.41778207500782
35	39.41945787181725
36	39.45710630790995
37	38.9291858165439
38	38.95545111564741
39	38.98454644403401
40	38.902159341975846
41	38.97204177071673
42	39.453461453827195
43	38.9407155378865
44	39.40543054822116
45	39.427057223069504
46	38.96618911202883
47	38.918796857722924
48	38.991276333416586
49	39.42861500114868
50	39.37966889680363
51	39.44413084641149
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1136.0
1	1596.0
2	2056.0
3	2389.0
4	2722.0
5	2819.5
6	2917.0
7	3566.5
8	4216.0
9	5471.5
10	6727.0
11	9988.5
12	13250.0
13	20565.5
14	27881.0
15	42405.0
16	56929.0
17	76454.5
18	95980.0
19	129237.0
20	162494.0
21	208592.0
22	254690.0
23	327801.5
24	400913.0
25	506815.5
26	752589.0
27	892460.0
28	1056463.0
29	1220466.0
30	1405656.5
31	1590847.0
32	1762751.0
33	1934655.0
34	2078597.5
35	2222540.0
36	2288969.0
37	2355398.0
38	2359248.0
39	2363098.0
40	2282188.0
41	2201278.0
42	2074662.0
43	1948046.0
44	1798643.0
45	1649240.0
46	1482653.5
47	1316067.0
48	1168632.0
49	1021197.0
50	863869.0
51	706541.0
52	652006.0
53	597471.0
54	458847.0
55	320223.0
56	261577.5
57	202932.0
58	158866.5
59	114801.0
60	90768.0
61	66735.0
62	56563.5
63	46392.0
64	37797.5
65	29203.0
66	23106.5
67	17010.0
68	12409.0
69	7808.0
70	5590.0
71	3372.0
72	2627.5
73	1883.0
74	1545.5
75	1073.5
76	939.0
77	777.0
78	615.0
79	530.0
80	445.0
81	376.5
82	308.0
83	226.5
84	145.0
85	116.0
86	87.0
87	64.5
88	42.0
89	31.0
90	20.0
91	15.5
92	11.0
93	6.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047524082327251006
2	0.0
3	0.0
4	0.0
5	1.225584518024181E-4
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	8.170563453494542E-6
16	0.0
17	3.2682253813978166E-5
18	0.0
19	0.002263246076617988
20	6.985831752737834E-4
21	0.001977276355745679
22	7.353507108145089E-5
23	0.001115281911402005
24	0.004073025881567029
25	0.0016667949445128866
26	0.11335839735378327
27	0.10562087376332394
28	0.12382488913770977
29	0.742361054257607
30	0.1105518088075079
31	0.006491512663801414
32	0.07575337905907464
33	0.0014584455764487757
34	0.009808761425920198
35	0.0022346491045307573
36	0.019074180382183007
37	0.0246219929671058
38	0.006213713506382598
39	0.007120646049720493
40	0.01782816945552509
41	0.01764841705954821
42	0.007279972037063637
43	0.011986216586276494
44	0.03339309283443219
45	0.002422572063961132
46	0.016292103526268115
47	0.026811703972642337
48	0.010266312979315892
49	0.027085417848334403
50	0.024213464794431074
51	0.02402962711672745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.4478116E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	63.20310873638193
#Duplication Level	Relative count
1	100.0
2	70.38419954238013
3	33.890271806089864
4	13.110055065248549
5	4.537225616655352
6	1.5388096854491966
7	0.6763722310226045
8	0.4651630585099696
9	0.3532724849764904
10++	26.48412159613789
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC	108341	0.44260350755752603	TruSeq Adapter, Index 6 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GAAGA	2671980	2.2401228	6.691985	6
GGAAG	1404935	1.8715601	9.035239	5
AAGAG	1967710	1.6496805	6.08366	7
GCCAA	1241215	1.5956583	8.329102	34
CTCCA	1249265	1.5567942	8.041578	24
CGCCA	708195	1.4211087	11.938006	33
TCCAG	1106790	1.4040067	8.088461	25
AGAGC	1019565	1.3342437	8.265487	8
CTGAA	1612105	1.3101339	5.5650573	19
CCAAT	1623900	1.2964457	5.4646635	35
CCAGT	1003320	1.272751	7.901128	26
GAGCA	957975	1.2536445	8.248592	9
TGAAC	1520470	1.2356634	5.5033445	20
CGGAA	942640	1.2335765	8.16842	4
ATGCC	964035	1.2229164	7.858192	47
AGCAC	915855	1.1773881	7.9971986	10
CACAC	907630	1.146237	7.8626866	12
ACTCC	896515	1.1172084	7.6244917	23
TCTGA	1382815	1.1089131	5.300598	18
GAACT	1326230	1.0778074	5.3414025	21
ACGCC	507790	1.0189635	11.500196	32
ATCTC	1267390	0.99842715	5.0736732	40
GCACA	774700	0.9959246	7.8333316	11
AACTC	1213360	0.96868986	5.1269855	22
GTCTG	758530	0.9665293	7.660772	17
CACGC	477065	0.9573087	11.455292	31
GTCAC	748330	0.9492861	7.6147475	29
TCTCG	756370	0.9467803	7.430053	41
CGTCT	755125	0.9452219	7.485845	16
ATCGG	706715	0.9125898	7.8744407	2
TATGC	1130665	0.9067078	5.0195575	46
CTCGT	723820	0.9060361	7.3793135	42
ACGTC	706015	0.8956078	7.582365	15
CAGTC	699700	0.887597	7.548313	27
TCGGA	679875	0.87793094	7.8671656	3
AGTCA	1059410	0.86096686	5.104779	28
GTATG	1052230	0.858957	5.0951724	45
GATCG	646555	0.83490443	7.639897	1
CACGT	625150	0.7930274	7.4666696	14
TCACG	617680	0.7835514	7.4009476	30
ACACG	560065	0.71999806	7.5318565	13
>>END_MODULE