FastQCFastQC Report
Wed 23 Jul 2014
DGY952_1_ACAGTG_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename DGY952_1_ACAGTG_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 19533739
Filtered Sequences 0
Sequence length 51
%GC 38

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC 73071 0.3740758489708499 TruSeq Adapter, Index 5 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GAAGA 2118300 2.2404923 6.11229 6
GGAAG 1099250 1.8328303 7.9426928 5
AAGAG 1513095 1.6003767 5.4193254 7
CTCCA 1015695 1.5425538 6.928196 24
TCCAG 896305 1.3986317 6.9634833 25
AGAGC 783225 1.2709892 7.121954 8
CCAGT 809515 1.2632009 6.8053875 26
GAGCA 757900 1.2298927 7.109764 9
CAGTG 756690 1.2132103 6.92534 35
ATGCC 766765 1.1964921 6.766376 47
CACAC 767335 1.1795036 6.6151996 12
CGGAA 718920 1.1666372 7.091488 4
AGCAC 737850 1.1653405 6.8870325 10
CACAG 687540 1.0858822 6.720465 33
ACTCC 694935 1.0554099 6.5149546 23
GCACA 622300 0.9828439 6.6986914 11
GTCTG 608405 0.9637706 6.6256967 17
GTCAC 605565 0.94494885 6.513941 29
CGTCT 609885 0.94028234 6.4172826 16
TCTCG 581345 0.89628124 6.3525734 41
ACGTC 567020 0.88480175 6.4797015 15
CTCGT 572710 0.88296825 6.3325 42
CAGTC 564485 0.880846 6.4629045 27
ATCGG 542265 0.8694201 6.763351 2
TCGGA 524115 0.84031993 6.7618446 3
GATCG 501400 0.8039008 6.5455465 1
CACGT 497170 0.7758049 6.370776 14
ACACG 442905 0.69951224 6.4180365 13
Produced by FastQC (version 0.10.1)