##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	DGY952_1_ACAGTG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	19533739	
Filtered Sequences	0	
Sequence length	51	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.261194029468705	34.0	33.0	34.0	31.0	34.0
2	33.412322392553726	34.0	34.0	34.0	31.0	34.0
3	33.41927410824932	34.0	34.0	34.0	31.0	34.0
4	36.66082166860118	37.0	37.0	37.0	35.0	37.0
5	36.6072394025537	37.0	37.0	37.0	35.0	37.0
6	36.612827272853394	37.0	37.0	37.0	35.0	37.0
7	36.571021502846946	37.0	37.0	37.0	35.0	37.0
8	36.57090938913436	37.0	37.0	37.0	35.0	37.0
9	38.44976499378844	39.0	39.0	39.0	37.0	39.0
10	38.43769715567511	39.0	39.0	39.0	37.0	39.0
11	38.44725763971762	39.0	39.0	39.0	37.0	39.0
12	38.438518094257326	39.0	39.0	39.0	37.0	39.0
13	38.41809507130202	39.0	39.0	39.0	37.0	39.0
14	40.07742096891947	41.0	40.0	41.0	38.0	41.0
15	40.049260052056596	41.0	40.0	41.0	38.0	41.0
16	40.0356646518109	41.0	40.0	41.0	38.0	41.0
17	40.026281809130346	41.0	40.0	41.0	38.0	41.0
18	40.00553898053005	41.0	40.0	41.0	38.0	41.0
19	40.01578187360853	41.0	40.0	41.0	38.0	41.0
20	39.979716888814785	41.0	40.0	41.0	38.0	41.0
21	39.96499436180651	41.0	40.0	41.0	38.0	41.0
22	39.9331173617094	41.0	40.0	41.0	38.0	41.0
23	39.90054413033777	41.0	40.0	41.0	38.0	41.0
24	39.87063408597811	41.0	40.0	41.0	38.0	41.0
25	39.845235722664256	41.0	40.0	41.0	38.0	41.0
26	39.73494311560117	41.0	40.0	41.0	38.0	41.0
27	39.66958629886475	41.0	40.0	41.0	38.0	41.0
28	39.60904228320037	41.0	40.0	41.0	38.0	41.0
29	39.30808643445067	41.0	40.0	41.0	37.0	41.0
30	39.32887400614905	41.0	39.0	41.0	37.0	41.0
31	39.600702814755536	41.0	40.0	41.0	38.0	41.0
32	39.62846432011813	41.0	40.0	41.0	38.0	41.0
33	39.659604799675066	41.0	40.0	41.0	38.0	41.0
34	39.75236169583304	41.0	40.0	41.0	38.0	41.0
35	39.79978600103134	41.0	40.0	41.0	38.0	41.0
36	39.802204688001616	41.0	40.0	41.0	38.0	41.0
37	39.77310063372916	41.0	40.0	41.0	38.0	41.0
38	39.72961878931627	41.0	40.0	41.0	38.0	41.0
39	39.72723634732705	41.0	40.0	41.0	38.0	41.0
40	39.68978110130375	41.0	40.0	41.0	38.0	41.0
41	39.66254361236218	41.0	40.0	41.0	38.0	41.0
42	39.643429913750765	41.0	40.0	41.0	38.0	41.0
43	39.60725209853577	41.0	40.0	41.0	37.0	41.0
44	39.5678339922531	41.0	40.0	41.0	37.0	41.0
45	39.51030783200287	41.0	40.0	41.0	37.0	41.0
46	39.48473658832034	41.0	40.0	41.0	37.0	41.0
47	39.436052002128214	41.0	40.0	41.0	37.0	41.0
48	39.402683480105885	41.0	39.0	41.0	37.0	41.0
49	39.36110198871808	41.0	39.0	41.0	37.0	41.0
50	39.299956091355575	41.0	39.0	41.0	36.0	41.0
51	38.391288324268075	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	6.0
11	14.0
12	14.0
13	19.0
14	8.0
15	22.0
16	37.0
17	73.0
18	164.0
19	373.0
20	1158.0
21	1856.0
22	2965.0
23	5087.0
24	8675.0
25	13105.0
26	18636.0
27	27209.0
28	38239.0
29	51612.0
30	68979.0
31	91308.0
32	119892.0
33	160051.0
34	219510.0
35	315135.0
36	494993.0
37	932183.0
38	2585025.0
39	1.435607E7
40	21314.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.690887993062294	31.188076874459785	32.04767910368718	21.073356028790737
2	18.87210635915633	31.525904999549752	32.26286068427555	17.339127957018366
3	17.11924685796201	30.91876061208763	32.96380687793566	18.9981856520147
4	17.731065209789072	29.844828990496904	32.58767817057451	19.83642762913951
5	19.66577886826847	30.063033583884764	31.91258968980502	18.35859785804174
6	19.56993487012394	30.2548017048861	30.636955884380352	19.53830754060961
7	19.80311091491496	30.764627294344415	30.77954507327041	18.65271671747022
8	19.06757840882383	30.84866650465638	30.830513298042938	19.25324178847685
9	19.197231006311693	29.80673592495528	31.46592160364178	19.530111465091245
10	19.064773006335344	30.561486461962044	30.711672762700477	19.662067769002135
11	19.50717166846552	30.1047177910998	30.813368602908024	19.574741937526653
12	18.972041143787166	30.224756253782235	30.740433257555043	20.06276934487555
13	18.89064351684027	30.83069759455678	30.758627418949334	19.52003146965361
14	19.061506862562258	30.46795086183961	30.6941799519283	19.77636232366983
15	18.963241956045486	30.75455911203478	30.71291321712209	19.569285714797648
16	18.965032756913562	30.313674202363412	30.743310330910024	19.977982709813006
17	19.570757973321538	30.241948096459794	30.669638960495778	19.51765496972289
18	19.120993681752378	30.144597508956174	31.00949080972158	19.724917999569872
19	19.144525704989952	30.17349629310479	30.58069265001847	20.101285351886787
20	19.304527547637278	30.16365801304716	30.883837637907895	19.647976801407665
21	19.60218373315489	30.0717097061593	30.500765895682246	19.82534066500356
22	19.194935731647632	30.500995202142754	30.524042527485808	19.780026538723806
23	19.368316961543858	30.526486831673118	30.42217884261289	19.683017364170137
24	19.279358756954984	30.054353833220265	30.404700532234347	20.2615868775904
25	19.263824617261005	30.112567100398618	30.7867041791359	19.83690410320448
26	19.440348275392672	30.132661550219524	30.323974797165317	20.103015377222487
27	19.33601083875679	30.033748375885356	30.351573384204194	20.278667401153665
28	19.318925985263235	30.47810931645742	30.336028389326447	19.866936308952898
29	19.875580460324443	30.121984997650564	30.262894433203023	19.739540108821966
30	19.376831295047577	30.042812303391713	30.667971563151326	19.912384838409388
31	19.332505344561884	30.094584551027875	30.303211508671897	20.269698595738348
32	19.50116502586214	30.528590779798844	30.241934988073087	19.728309206265934
33	19.383713217174765	30.08777088311647	30.247348256743518	20.281167642965247
34	19.339277485945995	30.506200145403906	30.2750437747673	19.879478593882798
35	19.496229162406852	30.171830439936343	30.216287794510777	20.115652603146028
36	19.419053483034457	30.471097707248195	30.230032987668988	19.879815822048357
37	19.766745754562276	30.15367733111508	30.23772198185203	19.84185493247061
38	19.528972112665834	30.14552266295471	30.601525381287725	19.723979843091733
39	19.79901108004026	30.10567046991617	30.232792454800787	19.86252599524278
40	19.398867232886374	30.534861139357922	30.224429535589653	19.84184209216605
41	19.49569746353622	30.170980126306045	30.57959052428171	19.75373188587603
42	19.41297503973805	30.090070865741424	30.21352799085944	20.283426103661082
43	19.360726002009276	30.16750560609922	30.62446707625657	19.847301315634933
44	19.497629046388916	30.178519522855446	30.21746014935912	20.106391281396522
45	19.790219778532045	30.08862813759068	30.25945948713223	19.861692596745044
46	19.385034876860487	30.16443810065768	30.61918670990213	19.831340312579698
47	19.51305152710415	30.561700390545226	30.212760814960788	19.71248726738983
48	19.408709318219206	30.113885870014595	30.590038873634807	19.887365938131392
49	19.774034270414116	30.17861429643564	30.188149104322065	19.85920232882818
50	19.513990458354655	30.175626226482503	30.205182396955244	20.1052009182076
51	19.428947101343972	30.10522626837804	30.189244244783982	20.276582385494017
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	36.76424402185303
2	36.211234316174696
3	36.11743250997671
4	37.56749283892859
5	38.024376726310216
6	39.10824241073355
7	38.45582763238517
8	38.320820197300684
9	38.72734247140294
10	38.72684077533748
11	39.08191360599218
12	39.03481048866272
13	38.41067498649388
14	38.83786918623209
15	38.53252767084313
16	38.94301546672657
17	39.08841294304443
18	38.84591168132225
19	39.24581105687674
20	38.95250434904495
21	39.427524398158454
22	38.974962270371435
23	39.051334325713995
24	39.54094563454538
25	39.10072872046548
26	39.54336365261516
27	39.61467823991045
28	39.185862294216136
29	39.61512056914641
30	39.28921613345696
31	39.60220394030023
32	39.22947423212808
33	39.66488086014001
34	39.21875607982879
35	39.61188176555288
36	39.29886930508282
37	39.60860068703288
38	39.25295195575757
39	39.66153707528304
40	39.24070932505243
41	39.249429349412246
42	39.69640114339913
43	39.20802731764421
44	39.60402032778544
45	39.65191237527709
46	39.216375189440186
47	39.22553879449398
48	39.2960752563506
49	39.633236599242295
50	39.61919137656225
51	39.70552948683799
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7054.0
1	8745.5
2	10437.0
3	10382.0
4	10327.0
5	9340.5
6	8354.0
7	8341.0
8	8328.0
9	8495.5
10	8663.0
11	9874.5
12	11086.0
13	13533.5
14	15981.0
15	21174.5
16	26368.0
17	36056.5
18	45745.0
19	65153.0
20	84561.0
21	116368.5
22	148176.0
23	201430.5
24	254685.0
25	339087.0
26	535246.0
27	647003.0
28	782741.0
29	918479.0
30	1073041.5
31	1227604.0
32	1380261.5
33	1532919.0
34	1660974.5
35	1789030.0
36	1861710.5
37	1934391.0
38	1948336.5
39	1962282.0
40	1902526.5
41	1842771.0
42	1739666.0
43	1636561.0
44	1509276.0
45	1381991.0
46	1235586.0
47	1089181.0
48	962968.5
49	836756.0
50	706320.5
51	575885.0
52	518697.5
53	461510.0
54	358510.0
55	255510.0
56	206521.0
57	157532.0
58	123177.0
59	88822.0
60	70246.0
61	51670.0
62	42762.5
63	33855.0
64	27389.0
65	20923.0
66	16565.5
67	12208.0
68	9003.0
69	5798.0
70	4191.5
71	2585.0
72	2036.0
73	1487.0
74	1276.5
75	967.5
76	869.0
77	744.5
78	620.0
79	540.0
80	460.0
81	408.0
82	356.0
83	267.5
84	179.0
85	135.0
86	91.0
87	74.5
88	58.0
89	37.0
90	16.0
91	13.5
92	11.0
93	8.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.046724285606560015
2	0.0
3	0.0
4	0.0
5	1.2798369016807278E-4
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	5.119347606722912E-6
16	0.0
17	2.0477390426891647E-5
18	0.0
19	0.0021040518663631167
20	7.167086649412076E-4
21	0.0020477390426891645
22	9.726760452773531E-5
23	0.0012286434256134986
24	0.004105716780591775
25	0.0016535492769715002
26	0.11407442272060664
27	0.10648243021983655
28	0.12509125877027436
29	0.7479827594706779
30	0.11053183417675438
31	0.006491332765324652
32	0.07606838608829575
33	0.0013719851586017403
34	0.009926415009435726
35	0.0021142905615765626
36	0.019013257011368894
37	0.024746926330898558
38	0.006040830175933035
39	0.00675241949326752
40	0.017974029447204144
41	0.01740066251525118
42	0.007433292724961668
43	0.011943437966484552
44	0.03365971051420314
45	0.002247393599351358
46	0.016218093218098185
47	0.026328804741375937
48	0.01019774043259204
49	0.027086468187170927
50	0.02468549415961788
51	0.02347220877682455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.9533739E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	56.96244805656408
#Duplication Level	Relative count
1	100.0
2	64.17945193330968
3	26.636218517204682
4	8.606775452288046
5	2.482041504079461
6	0.9433753103937567
7	0.5210180915218162
8	0.3946434905995034
9	0.35473572188719404
10++	17.59156615821213
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC	73071	0.3740758489708499	TruSeq Adapter, Index 5 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GAAGA	2118300	2.2404923	6.11229	6
GGAAG	1099250	1.8328303	7.9426928	5
AAGAG	1513095	1.6003767	5.4193254	7
CTCCA	1015695	1.5425538	6.928196	24
TCCAG	896305	1.3986317	6.9634833	25
AGAGC	783225	1.2709892	7.121954	8
CCAGT	809515	1.2632009	6.8053875	26
GAGCA	757900	1.2298927	7.109764	9
CAGTG	756690	1.2132103	6.92534	35
ATGCC	766765	1.1964921	6.766376	47
CACAC	767335	1.1795036	6.6151996	12
CGGAA	718920	1.1666372	7.091488	4
AGCAC	737850	1.1653405	6.8870325	10
CACAG	687540	1.0858822	6.720465	33
ACTCC	694935	1.0554099	6.5149546	23
GCACA	622300	0.9828439	6.6986914	11
GTCTG	608405	0.9637706	6.6256967	17
GTCAC	605565	0.94494885	6.513941	29
CGTCT	609885	0.94028234	6.4172826	16
TCTCG	581345	0.89628124	6.3525734	41
ACGTC	567020	0.88480175	6.4797015	15
CTCGT	572710	0.88296825	6.3325	42
CAGTC	564485	0.880846	6.4629045	27
ATCGG	542265	0.8694201	6.763351	2
TCGGA	524115	0.84031993	6.7618446	3
GATCG	501400	0.8039008	6.5455465	1
CACGT	497170	0.7758049	6.370776	14
ACACG	442905	0.69951224	6.4180365	13
>>END_MODULE