##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	DGY1181_1_GGCTAC_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	14760280	
Filtered Sequences	0	
Sequence length	51	
%GC	37	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.301436964610424	34.0	33.0	34.0	31.0	34.0
2	33.443774372843876	34.0	34.0	34.0	31.0	34.0
3	33.46339581633953	34.0	34.0	34.0	31.0	34.0
4	36.672900514082386	37.0	37.0	37.0	35.0	37.0
5	36.624252114458535	37.0	37.0	37.0	35.0	37.0
6	36.62288452522581	37.0	37.0	37.0	35.0	37.0
7	36.61464342139851	37.0	37.0	37.0	35.0	37.0
8	36.60245828669917	37.0	37.0	37.0	35.0	37.0
9	38.46339452910107	39.0	39.0	39.0	37.0	39.0
10	38.470747573894265	39.0	39.0	39.0	37.0	39.0
11	38.45463114520863	39.0	39.0	39.0	37.0	39.0
12	38.44900584541757	39.0	39.0	39.0	37.0	39.0
13	38.45112816288038	39.0	39.0	39.0	37.0	39.0
14	40.08834635928316	41.0	40.0	41.0	38.0	41.0
15	40.072240635001506	41.0	40.0	41.0	38.0	41.0
16	40.047717116477465	41.0	40.0	41.0	38.0	41.0
17	40.035664567338834	41.0	40.0	41.0	38.0	41.0
18	39.999561051687365	41.0	40.0	41.0	38.0	41.0
19	40.041754221464636	41.0	40.0	41.0	38.0	41.0
20	40.0156845263098	41.0	40.0	41.0	38.0	41.0
21	39.98125204941912	41.0	40.0	41.0	38.0	41.0
22	39.95802383152623	41.0	40.0	41.0	38.0	41.0
23	39.91563723723398	41.0	40.0	41.0	38.0	41.0
24	39.873403282322556	41.0	40.0	41.0	38.0	41.0
25	39.83570033901796	41.0	40.0	41.0	38.0	41.0
26	39.761344838986794	41.0	40.0	41.0	38.0	41.0
27	39.69080505247868	41.0	40.0	41.0	38.0	41.0
28	39.641801713788624	41.0	40.0	41.0	38.0	41.0
29	39.37115989669573	41.0	40.0	41.0	37.0	41.0
30	39.3850376144626	41.0	40.0	41.0	37.0	41.0
31	39.62814553653454	41.0	40.0	41.0	38.0	41.0
32	39.63668019847862	41.0	40.0	41.0	38.0	41.0
33	39.66533256821686	41.0	40.0	41.0	38.0	41.0
34	39.755376998268325	41.0	40.0	41.0	38.0	41.0
35	39.789070261539756	41.0	40.0	41.0	38.0	41.0
36	39.7764846601826	41.0	40.0	41.0	38.0	41.0
37	39.767024948036216	41.0	40.0	41.0	38.0	41.0
38	39.732870921147835	41.0	40.0	41.0	38.0	41.0
39	39.70290990414816	41.0	40.0	41.0	38.0	41.0
40	39.63890014281572	41.0	40.0	41.0	38.0	41.0
41	39.630984439319576	41.0	40.0	41.0	38.0	41.0
42	39.613278271143905	41.0	40.0	41.0	38.0	41.0
43	39.574767687333846	41.0	40.0	41.0	37.0	41.0
44	39.52592715043346	41.0	40.0	41.0	37.0	41.0
45	39.446208405260606	41.0	40.0	41.0	37.0	41.0
46	39.41703273921633	41.0	40.0	41.0	37.0	41.0
47	39.386955599758274	41.0	40.0	41.0	37.0	41.0
48	39.34138844249567	41.0	39.0	41.0	37.0	41.0
49	39.273956862606944	41.0	39.0	41.0	36.0	41.0
50	39.22475948965738	41.0	39.0	41.0	36.0	41.0
51	38.32757623839114	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	23.0
10	73.0
11	129.0
12	202.0
13	161.0
14	189.0
15	234.0
16	288.0
17	347.0
18	485.0
19	713.0
20	1134.0
21	1898.0
22	3106.0
23	4978.0
24	7514.0
25	11112.0
26	15580.0
27	21474.0
28	29264.0
29	38797.0
30	50982.0
31	66122.0
32	85438.0
33	113792.0
34	158204.0
35	227172.0
36	361713.0
37	702828.0
38	2016281.0
39	1.0822711E7
40	17331.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.148773297000435	31.05842657058746	31.729508420057073	21.063291712355035
2	18.712443124385175	31.854233117528935	32.93604863864371	16.49727511944218
3	16.92961786632774	31.404790424029898	33.392943765294426	18.272647944347938
4	17.669603828653656	30.497016316763638	32.8647085285645	18.968671326018203
5	19.24934350838873	30.731788285859075	32.19332560086936	17.825542604882834
6	19.178789291260056	30.907516659575563	31.12226868324991	18.791425365914467
7	19.530395087356066	31.153921199326838	31.21290382025273	18.10277989306436
8	18.660987460942476	31.199333616977455	31.47128645256052	18.66839246951955
9	18.753207933724838	30.416733286902414	32.025720379288195	18.804338400084553
10	18.816580715270984	30.914670995401174	31.27444736820711	18.99430092112074
11	19.007478177920746	30.80522862709921	31.306723178693087	18.880570016286953
12	18.752957260973368	30.887395090066043	31.250992528597017	19.108655120363572
13	18.67429344158783	31.20520748928882	31.216101591568723	18.904397477554628
14	18.79404049245678	31.11261439484888	31.182125271336314	18.911219841358022
15	18.71157593216389	31.170899197034203	31.260768765904167	18.85675610489774
16	18.71020739443967	30.930856325218762	31.239664830206472	19.11927145013509
17	19.066908920525456	30.868864629477745	31.193282726323375	18.870943723673424
18	18.90663998243936	30.756056118176623	31.303057936570312	19.034245962813714
19	18.84782651408238	30.724468205472522	31.152539341311243	19.275165939133856
20	18.952350497416038	30.781082743687787	31.28427103008886	18.982295728807312
21	19.11714659450862	30.677610429339158	31.083916114712217	19.121326861440004
22	18.886761556529795	30.883601722732713	31.044716661687083	19.184920059050413
23	19.015061847002094	30.90157664631184	31.05481710130415	19.02854440538191
24	18.996097337410852	30.679292850927613	31.005325557730856	19.31928425393068
25	18.953297799615033	30.675549418720855	31.18360886983399	19.187543911830126
26	19.075500360989686	30.675440140683964	30.98492668936971	19.26413280895664
27	19.08243680756683	30.615917997353282	30.930662580967947	19.37098261411194
28	19.030938344093236	30.831112244204185	30.914774642633375	19.223174769069207
29	19.320112503391325	30.64817604626654	30.908275379726213	19.123436070615917
30	19.107181987017604	30.601916865692047	31.058105605531143	19.232795541759202
31	19.03611584604086	30.66991276588249	30.869610874590453	19.424360513486192
32	19.160579426578018	30.883790729047313	30.854052455007384	19.101577389367282
33	19.12988123472955	30.607163639489997	30.846748363817834	19.41620676196262
34	19.23524370643018	30.665110546649487	30.864361389497013	19.235284357423314
35	19.34780085766683	30.702323540405104	30.815553122749662	19.134322479178405
36	19.16462319326055	30.631910273630304	30.806486320652958	19.396980212456192
37	19.094931202272917	30.655167475077956	31.016422106273982	19.233479216375144
38	19.162861165760397	30.877166547401064	30.8300655552043	19.12990673163424
39	19.126984449611648	30.624176815883185	30.855576242432125	19.393262492073042
40	19.080173755814076	30.862120861200665	30.826126146027143	19.231579236958115
41	19.166308531401953	30.68650651340939	30.994488161074617	19.15269679411404
42	19.162400756139824	30.63538360132933	30.80769117940076	19.394524463130086
43	19.112034076051067	30.683140922378826	30.957101348691253	19.247723652878854
44	19.151135657799493	30.70483604125604	30.82335749526558	19.32067080567889
45	19.328737587637406	30.63626030884799	30.809441094001077	19.225561009513527
46	19.096720857425943	30.672644561086287	30.984601867570316	19.24603271391745
47	19.178553486369466	30.898802448153567	30.81530478780219	19.107339277674775
48	19.148141894959007	30.644078581689982	30.989765767679888	19.21801375567112
49	19.282382883541604	30.667430689399954	30.78536811534177	19.264818311716667
50	19.20535885416423	30.70911619976692	30.802239373507668	19.283285572561176
51	19.15339150334441	30.632404077546944	30.81687802089887	19.397326398209774
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.21206500935546
2	35.20971824382735
3	35.20226581067568
4	36.63827515467186
5	37.07488611327157
6	37.97021465717452
7	37.63317498042043
8	37.329379930462025
9	37.55754633380939
10	37.81088163639172
11	37.8880481942077
12	37.86161238133694
13	37.57869091914245
14	37.705260333814806
15	37.56833203706163
16	37.829478844574766
17	37.937852644198884
18	37.94088594525307
19	38.12299245321624
20	37.93464622622335
21	38.23847345594862
22	38.071681615580204
23	38.04360625238401
24	38.315381591341534
25	38.14084171144516
26	38.339633169946325
27	38.453419421678774
28	38.25411311316244
29	38.443548574007245
30	38.33997752877681
31	38.46047635952706
32	38.2621568159453
33	38.546087996692165
34	38.4705280638535
35	38.482123336845234
36	38.56160340571674
37	38.32841041864806
38	38.29276789739463
39	38.52024694168469
40	38.31175299277219
41	38.319005325515995
42	38.556925219269914
43	38.35975772892992
44	38.47180646347838
45	38.55429859715093
46	38.342753571343394
47	38.28589276404424
48	38.36615565063013
49	38.54720119525827
50	38.48864442672541
51	38.55071790155418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12604.0
1	15812.5
2	19021.0
3	19652.5
4	20284.0
5	19273.5
6	18263.0
7	19023.5
8	19784.0
9	20350.0
10	20916.0
11	24342.0
12	27768.0
13	33370.5
14	38973.0
15	47133.0
16	55293.0
17	67054.5
18	78816.0
19	97773.5
20	116731.0
21	145302.5
22	173874.0
23	220132.0
24	266390.0
25	330694.5
26	477125.5
27	559252.0
28	655305.5
29	751359.0
30	863700.0
31	976041.0
32	1072616.5
33	1169192.0
34	1251582.0
35	1333972.0
36	1367360.5
37	1400749.0
38	1403403.5
39	1406058.0
40	1352908.0
41	1299758.0
42	1221022.0
43	1142286.0
44	1051024.5
45	959763.0
46	858103.5
47	756444.0
48	668885.5
49	581327.0
50	490297.5
51	399268.0
52	337861.0
53	276454.0
54	238759.0
55	201064.0
56	156956.5
57	112849.0
58	87995.5
59	63142.0
60	51271.5
61	39401.0
62	34505.0
63	29609.0
64	24169.0
65	18729.0
66	14475.0
67	10221.0
68	7432.0
69	4643.0
70	3406.0
71	2169.0
72	1656.0
73	1143.0
74	893.5
75	564.5
76	485.0
77	375.5
78	266.0
79	205.5
80	145.0
81	112.0
82	79.0
83	52.5
84	26.0
85	18.0
86	10.0
87	8.5
88	7.0
89	5.5
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04296666458901864
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	4.064963537277071E-5
18	0.0
19	0.002371228730078291
20	0.0
21	0.003096147227559369
22	6.774939228795118E-5
23	0.003184221437533705
24	0.011097350456766403
25	0.0039972141449891195
26	0.05271580213925481
27	0.05205185809483289
28	0.055100580747790694
29	0.6131320002059581
30	0.04267534220218045
31	0.0
32	0.05587292381987333
33	0.0010636654589208335
34	0.0033400450397959927
35	2.0324817686385355E-5
36	0.007425333394759449
37	3.658467183549363E-4
38	0.0034281192497703297
39	0.0035229683989734615
40	0.017330294547257912
41	0.0064971667204145175
42	3.1842214375337054E-4
43	8.06217768226619E-4
44	0.023427739853173517
45	0.02726235545667155
46	0.003848165481955627
47	0.003963339448845144
48	0.002737075448433228
49	0.02237084933348148
50	0.0017547092602579356
51	0.006869788377998249
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.476028E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	49.22951392936239
#Duplication Level	Relative count
1	100.0
2	44.919643677105334
3	14.026999724492606
4	3.9094499035724124
5	1.2535586371567637
6	0.6465240150610708
7	0.39581228762971804
8	0.28652768849297455
9	0.18642666911562128
10++	18.046652585177704
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC	22083	0.14961098298948258	TruSeq Adapter, Index 11 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
ACGGC	275570	0.99067193	5.4120617	32
CGGCT	259170	0.9204412	5.337415	33
CACGG	228575	0.82172525	5.188248	31
>>END_MODULE