##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	DGY1179_1_GATCAG_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	12399780	
Filtered Sequences	0	
Sequence length	51	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.33171749821368	34.0	34.0	34.0	31.0	34.0
2	33.470751497203985	34.0	34.0	34.0	31.0	34.0
3	33.48743009956628	34.0	34.0	34.0	31.0	34.0
4	36.69155493081329	37.0	37.0	37.0	35.0	37.0
5	36.64419352601417	37.0	37.0	37.0	35.0	37.0
6	36.64347585199092	37.0	37.0	37.0	35.0	37.0
7	36.63538982143231	37.0	37.0	37.0	35.0	37.0
8	36.62235684826666	37.0	37.0	37.0	35.0	37.0
9	38.48499844352077	39.0	39.0	39.0	37.0	39.0
10	38.49387973012424	39.0	39.0	39.0	37.0	39.0
11	38.47792936648876	39.0	39.0	39.0	37.0	39.0
12	38.47462172715967	39.0	39.0	39.0	37.0	39.0
13	38.476294337480184	39.0	39.0	39.0	37.0	39.0
14	40.12119747285839	41.0	40.0	41.0	38.0	41.0
15	40.106473501949225	41.0	40.0	41.0	38.0	41.0
16	40.082388316566906	41.0	40.0	41.0	38.0	41.0
17	40.072307331259104	41.0	40.0	41.0	38.0	41.0
18	40.03904859602348	41.0	40.0	41.0	38.0	41.0
19	40.08113377817993	41.0	40.0	41.0	38.0	41.0
20	40.05806482050488	41.0	40.0	41.0	38.0	41.0
21	40.02413107329323	41.0	40.0	41.0	38.0	41.0
22	39.99872134828198	41.0	40.0	41.0	38.0	41.0
23	39.95831079261084	41.0	40.0	41.0	38.0	41.0
24	39.921233763824844	41.0	40.0	41.0	38.0	41.0
25	39.884603436512585	41.0	40.0	41.0	38.0	41.0
26	39.81429444715955	41.0	40.0	41.0	38.0	41.0
27	39.745030960226714	41.0	40.0	41.0	38.0	41.0
28	39.69424634953201	41.0	40.0	41.0	38.0	41.0
29	39.430841434283515	41.0	40.0	41.0	38.0	41.0
30	39.44446667602167	41.0	40.0	41.0	37.0	41.0
31	39.68937416631585	41.0	40.0	41.0	38.0	41.0
32	39.698301421476835	41.0	40.0	41.0	38.0	41.0
33	39.72760597365437	41.0	40.0	41.0	38.0	41.0
34	39.813545885491514	41.0	40.0	41.0	38.0	41.0
35	39.846606149463945	41.0	40.0	41.0	38.0	41.0
36	39.834853279654965	41.0	40.0	41.0	38.0	41.0
37	39.82505770263666	41.0	40.0	41.0	38.0	41.0
38	39.792659143952555	41.0	40.0	41.0	38.0	41.0
39	39.76628964384852	41.0	40.0	41.0	38.0	41.0
40	39.70359248309244	41.0	40.0	41.0	38.0	41.0
41	39.69606734958201	41.0	40.0	41.0	38.0	41.0
42	39.67979915772699	41.0	40.0	41.0	38.0	41.0
43	39.64134041087826	41.0	40.0	41.0	38.0	41.0
44	39.59586960413814	41.0	40.0	41.0	37.0	41.0
45	39.517200547106484	41.0	40.0	41.0	37.0	41.0
46	39.48954062088198	41.0	40.0	41.0	37.0	41.0
47	39.45899604670405	41.0	40.0	41.0	37.0	41.0
48	39.414214848973124	41.0	40.0	41.0	37.0	41.0
49	39.34664453724179	41.0	39.0	41.0	37.0	41.0
50	39.29775754085959	41.0	39.0	41.0	36.0	41.0
51	38.3981472251927	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	40.0
11	60.0
12	88.0
13	83.0
14	91.0
15	105.0
16	127.0
17	150.0
18	248.0
19	389.0
20	622.0
21	1079.0
22	1994.0
23	3371.0
24	5206.0
25	8115.0
26	11513.0
27	15792.0
28	22109.0
29	29582.0
30	38870.0
31	50910.0
32	66792.0
33	89664.0
34	125563.0
35	181258.0
36	292137.0
37	568222.0
38	1650921.0
39	9219605.0
40	15058.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.354831422601231	31.261002509096482	31.855997224664982	21.528168843637303
2	18.872625159478638	31.870871902566012	32.89439812641837	16.36210481153698
3	16.78378164773891	31.38612943132862	33.46877928479376	18.361309636138706
4	17.521157633441884	30.350094921038924	32.97293177782186	19.15581566769733
5	19.375513113942343	30.52163022247169	32.22031358620879	17.882543077377182
6	19.309745818070965	30.669197356727295	30.978017351920762	19.043039473280977
7	19.781197730927484	30.96752523028634	31.045349191679207	18.205927847106963
8	18.883093087135418	31.02075198108353	31.250264117589182	18.845890814191865
9	18.939311826500145	30.12290540638624	31.888896415904156	19.048886351209458
10	19.024514951071716	30.67649587331388	31.026695634922557	19.27229354069185
11	19.184864570177858	30.523936715006233	31.11960050904129	19.171598205774618
12	18.926174496644297	30.66617310952291	31.017937415018654	19.38971497881414
13	18.84814891877114	31.020993920859887	31.013953473368076	19.1169036870009
14	18.96347354549839	30.92950842676241	30.961775128268403	19.145242899470798
15	18.87121384411659	30.987969141387993	31.04037329694559	19.10044371754983
16	18.867108932577835	30.746674537774055	31.041728159693154	19.344488369954952
17	19.244289635208762	30.657075526438902	31.017759432286564	19.08087540606577
18	19.042313653951926	30.59297019785835	31.109785818780654	19.25493032940907
19	18.99916415687914	30.56609096399068	30.92278403181559	19.51196084731459
20	19.136331451041872	30.580010290505154	31.08684992798259	19.19680833047038
21	19.259279809759242	30.490257387305853	30.899493412850866	19.350969390084042
22	19.058747133313993	30.668848642446704	30.874328243642562	19.398075980596737
23	19.22394166880454	30.699445771703687	30.83239572098753	19.24421683850424
24	19.160772909874904	30.461025710886396	30.80762998829919	19.57057139093951
25	19.08878499585178	30.49441294667574	30.98767502109596	19.429127036376514
26	19.260844140685865	30.526112704166188	30.74541340942557	19.467629745722373
27	19.212042390500432	30.44745510303511	30.742061302554617	19.598441203909836
28	19.168221474078464	30.63706784598022	30.706882881324283	19.48782779861703
29	19.490720740691934	30.474810054232197	30.696198423179922	19.338270781895943
30	19.27809071145695	30.444867845079475	30.83173751276287	19.44530393070071
31	19.19685671842565	30.481564995507988	30.650285730875872	19.671292555190494
32	19.346105244811106	30.709741608447473	30.63973332946012	19.304419817281296
33	19.263566666688174	30.46471611793101	30.64093874862335	19.630778466757466
34	19.405406233693753	30.480878494829184	30.63402312533117	19.4796921461459
35	19.362822463434426	30.70375937375653	30.5892734531215	19.344144709687544
36	19.331761684734172	30.446234213279112	30.78738626987782	19.434617832108895
37	19.241849498634163	30.498717391220193	30.620615226398513	19.63881788374713
38	19.380744044613902	30.72389841930602	30.57846354054934	19.316893995530737
39	19.449377721563373	30.490147348817313	30.61355701382161	19.446917915797705
40	19.253325632943046	30.68606605773357	30.608478710464247	19.452129598859138
41	19.369643729907686	30.533356668510898	30.75463222093359	19.342367380647826
42	19.29857739793478	30.468994397706467	30.614078049843453	19.618350154515298
43	19.246114841447206	30.49748755754883	30.76431687348237	19.49208072752159
44	19.35746475810227	30.53384670932037	30.605356568404403	19.503331964172958
45	19.46863291820322	30.47647665493084	30.60874770046259	19.446142726403348
46	19.26753426235172	30.49499311414074	30.784605676890127	19.452866946617412
47	19.36419823530432	30.72214128130569	30.587819236642698	19.325841246747295
48	19.29389708679562	30.472136682957995	30.758843202502952	19.475123027743432
49	19.41534784504298	30.5338137139165	30.59339209162777	19.457446349412745
50	19.37064026657733	30.531001356984294	30.58892279512634	19.509435581312037
51	19.317062863009717	30.44600972098836	30.620048721887226	19.6168786941147
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	36.88300026623853
2	35.23472997101562
3	35.14509128387762
4	36.67697330113921
5	37.258056191319525
6	38.35278529135194
7	37.987125578034444
8	37.72898390132728
9	37.988198177709606
10	38.296808491763564
11	38.35646277595248
12	38.315889475458434
13	37.96505260577204
14	38.10871644496919
15	37.97165756166642
16	38.21159730253279
17	38.32516504127453
18	38.29724398336099
19	38.51112500419373
20	38.33313978151225
21	38.61024919984328
22	38.45682311391073
23	38.46815850730878
24	38.73134430081441
25	38.517912032228296
26	38.72847388640824
27	38.810483594410265
28	38.65604927269549
29	38.82899152258788
30	38.72339464215766
31	38.86814927361614
32	38.650525062092406
33	38.89434513344564
34	38.885098379839654
35	38.70696717312197
36	38.76637951684307
37	38.88066738238129
38	38.69763804014464
39	38.89629563736108
40	38.70545523180218
41	38.71201111055551
42	38.91692755245008
43	38.7381955689688
44	38.860796722275225
45	38.91477564460657
46	38.720401208969136
47	38.69003948205162
48	38.76902011453905
49	38.87279419445573
50	38.880075847889366
51	38.93394155712442
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3345.0
1	4564.0
2	5783.0
3	6218.5
4	6654.0
5	6598.0
6	6542.0
7	7231.0
8	7920.0
9	9117.0
10	10314.0
11	12854.5
12	15395.0
13	20013.5
14	24632.0
15	31594.5
16	38557.0
17	49014.5
18	59472.0
19	75516.5
20	91561.0
21	115426.0
22	139291.0
23	177605.5
24	215920.0
25	269422.5
26	392045.0
27	461165.0
28	543897.0
29	626629.0
30	720771.0
31	814913.0
32	899640.0
33	984367.0
34	1052930.0
35	1121493.0
36	1152247.0
37	1183001.0
38	1185284.0
39	1187567.0
40	1146625.0
41	1105683.0
42	1039391.0
43	973099.0
44	897891.5
45	822684.0
46	737004.0
47	651324.0
48	576646.0
49	501968.0
50	424590.5
51	347213.0
52	303947.0
53	260681.0
54	208928.5
55	157176.0
56	128235.5
57	99295.0
58	78275.0
59	57255.0
60	46338.5
61	35422.0
62	30619.5
63	25817.0
64	20825.0
65	15833.0
66	12473.0
67	9113.0
68	6766.5
69	4420.0
70	3224.0
71	2028.0
72	1578.0
73	1128.0
74	906.5
75	605.0
76	525.0
77	448.0
78	371.0
79	305.5
80	240.0
81	199.5
82	159.0
83	126.0
84	93.0
85	77.0
86	61.0
87	44.5
88	28.0
89	23.5
90	19.0
91	14.0
92	9.0
93	6.0
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03935553695307497
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.4193977635087072E-5
18	0.0
19	0.002612949584589404
20	0.0
21	0.002693596176706361
22	7.258193290526122E-5
23	0.0030403765228092755
24	0.012024406884638275
25	0.003943618354519193
26	0.05444451433815761
27	0.05343643193669566
28	0.056726812895067494
29	0.6107850300569849
30	0.043750776223449125
31	0.0
32	0.05613002811340201
33	0.0010000177422502656
34	0.00314521709256132
35	1.6129318423391385E-5
36	0.007338839882643079
37	4.19362279008176E-4
38	0.003354898232065408
39	0.00344360948339406
40	0.01725837071302878
41	0.006129141000888725
42	2.983923908327406E-4
43	7.500133066876993E-4
44	0.023290735803377156
45	0.026331112326186432
46	0.0036774846005332355
47	0.003830713125555453
48	0.002750048791188231
49	0.021492316799169017
50	0.0018226129818432264
51	0.006733990441765902
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.239978E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	46.43972984602719
#Duplication Level	Relative count
1	100.0
2	41.87086295125487
3	11.73393646191732
4	2.7839390816843355
5	0.8304661438776045
6	0.37026040484132944
7	0.21831391794889707
8	0.15369299823602353
9	0.1047906806154706
10++	16.584871718741812
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC	20524	0.16551906566084237	TruSeq Adapter, Index 9 (100% over 51bp)
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE