##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename DGY1167_1_ACTTGA_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11563417 Filtered Sequences 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.32924774744351 34.0 34.0 34.0 31.0 34.0 2 33.47065292205583 34.0 34.0 34.0 31.0 34.0 3 33.48768300926967 34.0 34.0 34.0 31.0 34.0 4 36.69017324204429 37.0 37.0 37.0 35.0 37.0 5 36.64187134304678 37.0 37.0 37.0 35.0 37.0 6 36.64165211718993 37.0 37.0 37.0 35.0 37.0 7 36.63321213790007 37.0 37.0 37.0 35.0 37.0 8 36.619109645531246 37.0 37.0 37.0 35.0 37.0 9 38.48165304425154 39.0 39.0 39.0 37.0 39.0 10 38.4902134031835 39.0 39.0 39.0 37.0 39.0 11 38.47270741857705 39.0 39.0 39.0 37.0 39.0 12 38.46992190976076 39.0 39.0 39.0 37.0 39.0 13 38.47154781324586 39.0 39.0 39.0 37.0 39.0 14 40.11283351625216 41.0 40.0 41.0 38.0 41.0 15 40.09716280231008 41.0 40.0 41.0 38.0 41.0 16 40.07191066446882 41.0 40.0 41.0 38.0 41.0 17 40.06149073409702 41.0 40.0 41.0 38.0 41.0 18 40.0295745626055 41.0 40.0 41.0 38.0 41.0 19 40.07036890566171 41.0 40.0 41.0 38.0 41.0 20 40.047150855149475 41.0 40.0 41.0 38.0 41.0 21 40.01268664790001 41.0 40.0 41.0 38.0 41.0 22 39.9893263383998 41.0 40.0 41.0 38.0 41.0 23 39.94887531946656 41.0 40.0 41.0 38.0 41.0 24 39.91077101171739 41.0 40.0 41.0 38.0 41.0 25 39.87339970529472 41.0 40.0 41.0 38.0 41.0 26 39.80160561536439 41.0 40.0 41.0 38.0 41.0 27 39.730532073694135 41.0 40.0 41.0 38.0 41.0 28 39.683154382480545 41.0 40.0 41.0 38.0 41.0 29 39.41708303004207 41.0 40.0 41.0 37.0 41.0 30 39.43111876013811 41.0 40.0 41.0 37.0 41.0 31 39.67506845078751 41.0 40.0 41.0 38.0 41.0 32 39.68538495152428 41.0 40.0 41.0 38.0 41.0 33 39.713850239942055 41.0 40.0 41.0 38.0 41.0 34 39.80174770139311 41.0 40.0 41.0 38.0 41.0 35 39.837688634769464 41.0 40.0 41.0 38.0 41.0 36 39.82370617612424 41.0 40.0 41.0 38.0 41.0 37 39.81499395896559 41.0 40.0 41.0 38.0 41.0 38 39.780693890049974 41.0 40.0 41.0 38.0 41.0 39 39.750516218519145 41.0 40.0 41.0 38.0 41.0 40 39.689726401806666 41.0 40.0 41.0 38.0 41.0 41 39.679181335413226 41.0 40.0 41.0 38.0 41.0 42 39.66343780562441 41.0 40.0 41.0 38.0 41.0 43 39.62494295587541 41.0 40.0 41.0 37.0 41.0 44 39.57537438976731 41.0 40.0 41.0 37.0 41.0 45 39.496559970119556 41.0 40.0 41.0 37.0 41.0 46 39.46959112518385 41.0 40.0 41.0 37.0 41.0 47 39.43922051760306 41.0 40.0 41.0 37.0 41.0 48 39.39126998533392 41.0 39.0 41.0 37.0 41.0 49 39.322347364970064 41.0 39.0 41.0 37.0 41.0 50 39.271445455958215 41.0 39.0 41.0 36.0 41.0 51 38.369322752954425 40.0 38.0 41.0 34.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 9.0 10 29.0 11 49.0 12 72.0 13 68.0 14 75.0 15 88.0 16 87.0 17 129.0 18 178.0 19 297.0 20 585.0 21 996.0 22 1820.0 23 3059.0 24 4975.0 25 7495.0 26 10974.0 27 15498.0 28 20738.0 29 28121.0 30 37180.0 31 48495.0 32 63536.0 33 85377.0 34 118587.0 35 171159.0 36 274913.0 37 537420.0 38 1554754.0 39 8563236.0 40 13418.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.143843159782572 31.332607189173245 31.36561243108047 22.157937219963717 2 19.0136358482964 31.504874380989634 32.4870494595153 16.994440311198673 3 17.09710027753907 31.026685278235668 33.07093396355074 18.805280480674526 4 17.72556502978315 30.241078394042177 32.73309264899813 19.30026392717654 5 19.409755784125053 30.437430389304478 32.11715014688132 18.035663679689144 6 19.353630505584984 30.585180833658427 30.95076481285765 19.11042384789894 7 19.847403237295687 30.78257923241893 30.985616102921824 18.384401427363557 8 18.971944019661315 30.80611898714714 31.21756311304868 19.004373880142868 9 19.051332318120153 30.040367825531156 31.723814855072686 19.18448500127601 10 19.198339037673726 30.46231922622872 30.89303966120049 19.446302074897066 11 19.28730927890951 30.37338357684411 30.954102926496553 19.38520421774982 12 19.156474249782743 30.528164814950458 30.80973383559548 19.505627099671315 13 19.000162322261662 30.819419553925968 30.81422212828613 19.366195995526237 14 19.151328711919668 30.74892136122048 30.78804474490542 19.311705181954434 15 19.093560320448532 30.753902587790442 30.85689982467985 19.295637267081176 16 19.021150927965323 30.634136951041373 30.87838136426283 19.46633075673047 17 19.295775634485896 30.56741791807733 30.86573804265642 19.27106840478035 18 19.20149554409393 30.48655946594333 30.88130437568757 19.430640614275173 19 19.095162967369685 30.47590088611721 30.84336153241381 19.5855746140993 20 19.257542990968844 30.504815315403743 30.883751749158574 19.353889944468836 21 19.334019888181732 30.40497626577352 30.76787366099319 19.493130185051555 22 19.18024181448206 30.517771141903644 30.74543754826647 19.556549495347824 23 19.291362518577518 30.53644652982543 30.740536545984682 19.431654405612367 24 19.285602961264946 30.397265683718317 30.69553366062145 19.621597694395284 25 19.19383230271434 30.41450307594409 30.807361163937863 19.584303457403703 26 19.355376292960717 30.443842351787413 30.679974273820793 19.520807081431073 27 19.36002078008559 30.38012864513513 30.65143958940295 19.608410985376327 28 19.253026380663144 30.480673233955823 30.675198154795147 19.591102230585882 29 19.510659671449883 30.40408122955481 30.6400936601461 19.44516543884921 30 19.392706063491573 30.38054696477618 30.688162524145074 19.53858444758717 31 19.27782246372331 30.402094813323778 30.59203002019213 19.728052702760785 32 19.422393648401624 30.52839766228008 30.604585900269814 19.44462278904848 33 19.381068829119467 30.38259270351195 30.582241782593172 19.654096684775414 34 19.304257222319897 30.496171022611847 30.605277570916012 19.59429418415224 35 19.424855127165266 30.452408660865554 30.566336922727942 19.556399289241234 36 19.41311328411216 30.39927164565738 30.6462164250927 19.541398645137768 37 19.312177845777963 30.431664064627544 30.643081725244905 19.61307636434959 38 19.5336379304634 30.453783189929496 30.55062629329173 19.461952586315373 39 19.388312208667234 30.51961411821113 30.560286873078084 19.53178680004355 40 19.339581270787992 30.51098996813697 30.57940751271347 19.57002124836157 41 19.43722292275307 30.457896366407038 30.64281056435111 19.462070146488784 42 19.41886032996229 30.40414127977438 30.556916652930777 19.620081737332555 43 19.32513326622458 30.465428613927486 30.62014196614813 19.589296153699802 44 19.428722730379278 30.496752726345754 30.53662947691132 19.53789506636365 45 19.49200639464208 30.453864401305054 30.542010879818633 19.512118324234233 46 19.32135073963462 30.44386033689186 30.653642642238278 19.581146281235245 47 19.440583208329027 30.561150780489555 30.559153017810793 19.439112993370628 48 19.437238077958135 30.389650575737704 30.628177030372015 19.544934315932142 49 19.43959259139122 30.438846540594895 30.52544033215479 19.596120535859093 50 19.448512188631632 30.48626793654659 30.550117082712365 19.515102792109413 51 19.44073252106427 30.394948743745886 30.53720927034594 19.627109464843905 >>END_MODULE >>Per base GC content pass #Base %GC 1 37.30178037974628 2 36.00807615949507 3 35.902380758213596 4 37.02582895695969 5 37.4454194638142 6 38.464054353483924 7 38.23180466465924 8 37.97631789980419 9 38.23581731939616 10 38.644641112570795 11 38.672513496659334 12 38.66210134945406 13 38.3663583177879 14 38.463033893874105 15 38.38919758752971 16 38.487481684695794 17 38.56684403926624 18 38.63213615836911 19 38.68073758146898 20 38.61143293543768 21 38.827150073233284 22 38.73679130982989 23 38.723016924189885 24 38.90720065566023 25 38.77813576011804 26 38.87618337439179 27 38.968431765461915 28 38.844128611249026 29 38.95582511029909 30 38.93129051107875 31 39.005875166484095 32 38.86701643745011 33 39.035165513894874 34 38.89855140647214 35 38.9812544164065 36 38.95451192924993 37 38.925254210127555 38 38.99559051677877 39 38.920099008710785 40 38.90960251914956 41 38.89929306924185 42 39.03894206729485 43 38.91442941992438 44 38.96661779674292 45 39.00412471887631 46 38.902497020869866 47 38.87969620169965 48 38.98217239389028 49 39.03571312725032 50 38.96361498074105 51 39.067841985908174 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1136.0 1 1712.5 2 2289.0 3 2590.5 4 2892.0 5 2888.5 6 2885.0 7 3354.5 8 3824.0 9 4592.0 10 5360.0 11 7207.5 12 9055.0 13 12670.0 14 16285.0 15 22597.0 16 28909.0 17 38713.5 18 48518.0 19 63536.0 20 78554.0 21 100958.0 22 123362.0 23 157626.5 24 191891.0 25 244373.5 26 361719.0 27 426582.0 28 503105.5 29 579629.0 30 665107.5 31 750586.0 32 830883.5 33 911181.0 34 974010.5 35 1036840.0 36 1072423.5 37 1108007.0 38 1114603.5 39 1121200.0 40 1082843.5 41 1044487.0 42 983265.5 43 922044.0 44 850833.5 45 779623.0 46 698941.5 47 618260.0 48 548454.5 49 478649.0 50 411595.0 51 344541.0 52 286804.5 53 229068.0 54 190684.5 55 152301.0 56 123970.5 57 95640.0 58 75496.0 59 55352.0 60 45534.0 61 35716.0 62 31951.5 63 28187.0 64 22388.0 65 16589.0 66 12727.0 67 8865.0 68 6534.5 69 4204.0 70 3011.0 71 1818.0 72 1374.5 73 931.0 74 722.5 75 422.5 76 331.0 77 251.5 78 172.0 79 156.0 80 140.0 81 108.0 82 76.0 83 57.5 84 39.0 85 25.0 86 11.0 87 10.5 88 10.0 89 7.5 90 5.0 91 3.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04018708310873854 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0023695418058520246 20 0.0 21 0.0031910982713846607 22 6.053573956556267E-5 23 0.0033035217877207057 24 0.011510438480251987 25 0.004004006774122217 26 0.05463782893931785 27 0.053600073403908204 28 0.05658362056821094 29 0.6122930618172812 30 0.04461484005981969 31 0.0 32 0.05634147760994869 33 0.0011069392377702888 34 0.0035283688203927954 35 0.0 36 0.007895590031908388 37 4.323981397540191E-4 38 0.0033208177133108667 39 0.00340729734126167 40 0.017771563543890184 41 0.006546507835875849 42 3.0267869782781335E-4 43 8.388523911227971E-4 44 0.024179703975044747 45 0.02692975614388031 46 0.004133726216048423 47 0.004168318067228744 48 0.0029316593875322494 49 0.02198312142509433 50 0.0019371436660980054 51 0.007160513194326556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1.1563417E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Duplicate Percentage 43.84404924760602 #Duplication Level Relative count 1 100.0 2 39.201142425133355 3 10.272585996891932 4 2.3940526691587216 5 0.6745348397664749 6 0.33768742912344074 7 0.22428493426855392 8 0.13188290142383133 9 0.07392162627577807 10++ 14.693603259271704 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content pass >>END_MODULE