##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename DGY1163_1_ATCACG_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13698342 Filtered Sequences 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.337033927171625 34.0 34.0 34.0 31.0 34.0 2 33.47685712621279 34.0 34.0 34.0 31.0 34.0 3 33.49385407372659 34.0 34.0 34.0 31.0 34.0 4 36.69722299238842 37.0 37.0 37.0 35.0 37.0 5 36.65086314825546 37.0 37.0 37.0 35.0 37.0 6 36.64988040158437 37.0 37.0 37.0 35.0 37.0 7 36.64107481036756 37.0 37.0 37.0 35.0 37.0 8 36.627092388261296 37.0 37.0 37.0 35.0 37.0 9 38.488811492660936 39.0 39.0 39.0 37.0 39.0 10 38.49904645394311 39.0 39.0 39.0 37.0 39.0 11 38.48352669250045 39.0 39.0 39.0 37.0 39.0 12 38.47976258732626 39.0 39.0 39.0 37.0 39.0 13 38.480789280921734 39.0 39.0 39.0 37.0 39.0 14 40.125641263738345 41.0 40.0 41.0 38.0 41.0 15 40.111883102349175 41.0 40.0 41.0 38.0 41.0 16 40.086635959300764 41.0 40.0 41.0 38.0 41.0 17 40.07554731806229 41.0 40.0 41.0 38.0 41.0 18 40.043995762406865 41.0 40.0 41.0 38.0 41.0 19 40.085240826955555 41.0 40.0 41.0 38.0 41.0 20 40.06150357466619 41.0 40.0 41.0 38.0 41.0 21 40.028588277325824 41.0 40.0 41.0 38.0 41.0 22 40.00577332643615 41.0 40.0 41.0 38.0 41.0 23 39.96553955215894 41.0 40.0 41.0 38.0 41.0 24 39.928868471819435 41.0 40.0 41.0 38.0 41.0 25 39.89362435249463 41.0 40.0 41.0 38.0 41.0 26 39.82270044068107 41.0 40.0 41.0 38.0 41.0 27 39.754231862512995 41.0 40.0 41.0 38.0 41.0 28 39.70771667111246 41.0 40.0 41.0 38.0 41.0 29 39.439580425134665 41.0 40.0 41.0 38.0 41.0 30 39.455283274428396 41.0 40.0 41.0 37.0 41.0 31 39.6996153987103 41.0 40.0 41.0 38.0 41.0 32 39.71312615789561 41.0 40.0 41.0 38.0 41.0 33 39.74209294818307 41.0 40.0 41.0 38.0 41.0 34 39.82917268381823 41.0 40.0 41.0 38.0 41.0 35 39.863980545966804 41.0 40.0 41.0 38.0 41.0 36 39.85061469482949 41.0 40.0 41.0 38.0 41.0 37 39.84132079634163 41.0 40.0 41.0 38.0 41.0 38 39.80813203524923 41.0 40.0 41.0 38.0 41.0 39 39.78180848455966 41.0 40.0 41.0 38.0 41.0 40 39.7204496719384 41.0 40.0 41.0 38.0 41.0 41 39.711786287712776 41.0 40.0 41.0 38.0 41.0 42 39.696421216523866 41.0 40.0 41.0 38.0 41.0 43 39.65887192771213 41.0 40.0 41.0 38.0 41.0 44 39.611859376850134 41.0 40.0 41.0 37.0 41.0 45 39.534282689102085 41.0 40.0 41.0 37.0 41.0 46 39.51125450072717 41.0 40.0 41.0 37.0 41.0 47 39.48013839923109 41.0 40.0 41.0 37.0 41.0 48 39.433502755296956 41.0 40.0 41.0 37.0 41.0 49 39.364913213584536 41.0 39.0 41.0 37.0 41.0 50 39.3159778022771 41.0 39.0 41.0 36.0 41.0 51 38.416261325640725 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 12.0 11 17.0 12 21.0 13 26.0 14 29.0 15 43.0 16 43.0 17 55.0 18 109.0 19 243.0 20 511.0 21 986.0 22 1833.0 23 3188.0 24 5094.0 25 8121.0 26 11827.0 27 16823.0 28 23663.0 29 31630.0 30 41969.0 31 55871.0 32 73085.0 33 97747.0 34 136758.0 35 197831.0 36 317822.0 37 625389.0 38 1825969.0 39 1.0205347E7 40 16276.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.462144135771162 31.52620811386399 31.50886308874219 21.50278466162266 2 19.02022157134053 31.49459255725985 32.76887086042968 16.71631501096994 3 16.95399341029739 30.90119957583188 33.360102996406425 18.784704017464303 4 17.79591281923024 30.21516764583626 32.83688639106835 19.15203314386515 5 19.472619387076186 30.437800428694217 32.02332077852926 18.06625940570034 6 19.320841894588412 30.560209403444595 30.948979080825982 19.16996962114101 7 19.94619494826454 30.757240547797682 30.92337014216757 18.373194361770206 8 19.042990750267442 30.757028843344692 31.175590447369473 19.024389959018396 9 18.998182407768766 30.094364704867203 31.696303100039408 19.211149787324626 10 19.210062064445463 30.469534196182284 30.92715162170721 19.393252117665043 11 19.25506751109003 30.400117036061737 31.02100239576439 19.323813057083843 12 19.13575380144546 30.543440950736954 30.89778310396981 19.423022143847774 13 19.03455907291554 30.75034190269158 30.905981176408066 19.309117847984815 14 19.157581260564235 30.77844019371103 30.8428494484953 19.22112909722943 15 19.092135383975666 30.70735129842721 30.880233534832175 19.320279782764953 16 19.06667974854183 30.63901456103228 30.89138086930521 19.40292482112069 17 19.29736741824192 30.530086127224177 30.900590874514723 19.271955580019185 18 19.244453087826248 30.429901662551572 30.846908333869894 19.478736915752286 19 19.173946996430058 30.453885071984686 30.81951554473385 19.552652386851406 20 19.324440870289266 30.45604351241924 30.812349406957424 19.40716621033407 21 19.32398943430982 30.370650380400765 30.75830041958189 19.547059765707527 22 19.244407330439405 30.451625026198442 30.718781621092145 19.58518602227001 23 19.375828958457316 30.484974644310753 30.691122851424364 19.448073545807564 24 19.36278505686444 30.37184074379392 30.63426167637351 19.631112522968127 25 19.300598156107547 30.37953937292822 30.70345360066849 19.616408870295746 26 19.45442481579915 30.394181127390862 30.62792998665816 19.52346407015183 27 19.421694895941602 30.31098827174606 30.61247386031713 19.65484297199521 28 19.356785674111755 30.40997161527078 30.59796383759202 19.63527887302545 29 19.56964463828996 30.331907600651952 30.576489962148017 19.521957798910066 30 19.453564180712537 30.320165004640227 30.57874978792703 19.64752102672021 31 19.381805476896403 30.389057303431322 30.525548274382402 19.703588945289876 32 19.522531267964062 30.471194321914492 30.513946501705924 19.492327908415525 33 19.46872885443441 30.36477850415854 30.47739911578183 19.689093525625225 34 19.40952580079592 30.44423250560379 30.518879136846948 19.62736255675334 35 19.548586212009177 30.42869205840328 30.532660852872624 19.490060876714924 36 19.49594527891489 30.36915531103595 30.473387965738794 19.66151144431036 37 19.45185534945185 30.460060611980396 30.433130227571603 19.654953810996155 38 19.55134817625964 30.43098480212717 30.460208392010475 19.557458629602714 39 19.509220118278225 30.41296487512805 30.463658880737093 19.61415612585663 40 19.438396589567194 30.461613318538593 30.46203680426862 19.637953287625592 41 19.536307006608904 30.45597986482861 30.48491231016049 19.522800818402 42 19.47016195222487 30.37956756528676 30.472630214890184 19.67764026759819 43 19.416366165160824 30.44766718459314 30.4902931857092 19.64567346453683 44 19.5288705970629 30.46422907737317 30.444199878408227 19.562700447155702 45 19.522159752047074 30.390298027643876 30.459172217238482 19.628370003070568 46 19.455695263637114 30.436739766653954 30.49229626315762 19.615268706551316 47 19.56077204436754 30.5135247641184 30.450463367665463 19.475239823848597 48 19.495545824647717 30.38404185623905 30.479567210646515 19.640845108466724 49 19.472784204307093 30.44053231100149 30.425746137687238 19.66093734700418 50 19.534987011672957 30.46804626257905 30.456628594877134 19.540338130870854 51 19.496920501019247 30.35622636131727 30.465561314817062 19.68129182284642 >>END_MODULE >>Per base GC content pass #Base %GC 1 36.96492879739382 2 35.73653658231047 3 35.73869742776169 4 36.947945963095385 5 37.53887879277652 6 38.490811515729426 7 38.319389310034744 8 38.06738070928584 9 38.209332195093396 10 38.6033141821105 11 38.57888056817387 12 38.558775945293235 13 38.34367692090036 14 38.378710357793665 15 38.412415166740615 16 38.46960456966252 17 38.5693229982611 18 38.72319000357854 19 38.72659938328147 20 38.731607080623334 21 38.871049200017346 22 38.82959335270941 23 38.823902504264886 24 38.99389757983257 25 38.91700702640329 26 38.97788888595098 27 39.07653786793681 28 38.992064547137204 29 39.091602437200024 30 39.101085207432746 31 39.085394422186276 32 39.01485917637959 33 39.157822380059635 34 39.03688835754926 35 39.038647088724105 36 39.157456723225245 37 39.106809160448 38 39.10880680586236 39 39.12337624413485 40 39.07634987719279 41 39.059107825010905 42 39.147802219823056 43 39.06203962969766 44 39.0915710442186 45 39.15052975511764 46 39.07096397018843 47 39.036011868216136 48 39.13639093311444 49 39.13372155131127 50 39.07532514254382 51 39.178212323865665 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2682.0 1 3685.5 2 4689.0 3 4999.0 4 5309.0 5 5386.0 6 5463.0 7 6562.0 8 7661.0 9 9035.0 10 10409.0 11 13642.5 12 16876.0 13 20976.5 14 25077.0 15 32345.5 16 39614.0 17 49776.5 18 59939.0 19 76575.5 20 93212.0 21 117172.0 22 141132.0 23 179511.5 24 217891.0 25 277286.0 26 411482.0 27 486283.0 28 574852.0 29 663421.0 30 767070.5 31 870720.0 32 965121.0 33 1059522.0 34 1138803.5 35 1218085.0 36 1260262.0 37 1302439.0 38 1319259.5 39 1336080.0 40 1292467.5 41 1248855.0 42 1180051.0 43 1111247.0 44 1025533.0 45 939819.0 46 842696.5 47 745574.0 48 662054.0 49 578534.0 50 491632.5 51 404731.0 52 344033.5 53 283336.0 54 234617.5 55 185899.0 56 151614.5 57 117330.0 58 92393.0 59 67456.0 60 54421.0 61 41386.0 62 35740.5 63 30095.0 64 24633.5 65 19172.0 66 14916.0 67 10660.0 68 7925.5 69 5191.0 70 3852.0 71 2513.0 72 1922.5 73 1332.0 74 1052.5 75 658.5 76 544.0 77 428.5 78 313.0 79 251.5 80 190.0 81 161.0 82 132.0 83 86.5 84 41.0 85 26.0 86 11.0 87 12.5 88 14.0 89 9.0 90 4.0 91 3.5 92 3.0 93 1.5 94 0.0 95 0.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04128236833333552 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.4600307102859602E-5 18 0.0 19 0.0026207551249632986 20 0.0 21 0.0030660644916005162 22 6.570138196286821E-5 23 0.003139066027114814 24 0.012008752592102023 25 0.004234089059829285 26 0.05533516391983789 27 0.05454674733628347 28 0.05753981029236969 29 0.6170308786275011 30 0.044684239888301815 31 0.0 32 0.05702879954376961 33 0.0010147213436487424 34 0.0034310721691720066 35 7.300153551429801E-6 36 0.00802286875302135 37 4.3800921308578806E-4 38 0.0036792773899206196 39 0.0034310721691720066 40 0.018140881575303053 41 0.006694240806661127 42 3.1390660271148144E-4 43 9.49019961685874E-4 44 0.024353312247569817 45 0.02779168457029325 46 0.004190288138520706 47 0.004234089059829285 48 0.0029784626489833587 49 0.02273997831270383 50 0.0019929419195403356 51 0.007183351094606924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1.3698342E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Duplicate Percentage 50.951201601831706 #Duplication Level Relative count 1 100.0 2 45.4737351100531 3 13.51044737258111 4 3.671226252801309 5 1.202354494648453 6 0.6406977933586885 7 0.4296115827746213 8 0.2966365690586671 9 0.23898306660839325 10++ 20.515347920289383 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content pass >>END_MODULE