##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename DGY1162_1_CTTGTA_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12608334 Filtered Sequences 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.34558618133054 34.0 34.0 34.0 31.0 34.0 2 33.483380912973914 34.0 34.0 34.0 31.0 34.0 3 33.500070350293704 34.0 34.0 34.0 31.0 34.0 4 36.70077616915923 37.0 37.0 37.0 35.0 37.0 5 36.65344826683684 37.0 37.0 37.0 35.0 37.0 6 36.653253712980636 37.0 37.0 37.0 35.0 37.0 7 36.64468549135834 37.0 37.0 37.0 35.0 37.0 8 36.63048377366907 37.0 37.0 37.0 35.0 37.0 9 38.49448277623356 39.0 39.0 39.0 37.0 39.0 10 38.5029052212608 39.0 39.0 39.0 37.0 39.0 11 38.487295863196515 39.0 39.0 39.0 37.0 39.0 12 38.48402421763256 39.0 39.0 39.0 37.0 39.0 13 38.485826358978116 39.0 39.0 39.0 37.0 39.0 14 40.13130354890662 41.0 40.0 41.0 38.0 41.0 15 40.117429154398984 41.0 40.0 41.0 38.0 41.0 16 40.092099558910796 41.0 40.0 41.0 38.0 41.0 17 40.08196388198473 41.0 40.0 41.0 38.0 41.0 18 40.050182760069646 41.0 40.0 41.0 38.0 41.0 19 40.092390239662116 41.0 40.0 41.0 38.0 41.0 20 40.06943946757755 41.0 40.0 41.0 38.0 41.0 21 40.036374036411154 41.0 40.0 41.0 38.0 41.0 22 40.01284499601613 41.0 40.0 41.0 38.0 41.0 23 39.97388560613956 41.0 40.0 41.0 38.0 41.0 24 39.93541660619079 41.0 40.0 41.0 38.0 41.0 25 39.89969277463621 41.0 40.0 41.0 38.0 41.0 26 39.83009579219586 41.0 40.0 41.0 38.0 41.0 27 39.76109754072188 41.0 40.0 41.0 38.0 41.0 28 39.714105923907155 41.0 40.0 41.0 38.0 41.0 29 39.448365977614486 41.0 40.0 41.0 38.0 41.0 30 39.46470128408718 41.0 40.0 41.0 37.0 41.0 31 39.7075263075994 41.0 40.0 41.0 38.0 41.0 32 39.71960062288959 41.0 40.0 41.0 38.0 41.0 33 39.74955049572767 41.0 40.0 41.0 38.0 41.0 34 39.83595358435143 41.0 40.0 41.0 38.0 41.0 35 39.87160643111136 41.0 40.0 41.0 38.0 41.0 36 39.85753050323699 41.0 40.0 41.0 38.0 41.0 37 39.84864772776483 41.0 40.0 41.0 38.0 41.0 38 39.81697661245332 41.0 40.0 41.0 38.0 41.0 39 39.79069582071668 41.0 40.0 41.0 38.0 41.0 40 39.73135142200389 41.0 40.0 41.0 38.0 41.0 41 39.72366103245679 41.0 40.0 41.0 38.0 41.0 42 39.70838756333708 41.0 40.0 41.0 38.0 41.0 43 39.67138497441454 41.0 40.0 41.0 38.0 41.0 44 39.625277931247695 41.0 40.0 41.0 37.0 41.0 45 39.54753038744056 41.0 40.0 41.0 37.0 41.0 46 39.52318347531085 41.0 40.0 41.0 37.0 41.0 47 39.49436388661658 41.0 40.0 41.0 37.0 41.0 48 39.448357649789415 41.0 40.0 41.0 37.0 41.0 49 39.38120627197852 41.0 39.0 41.0 37.0 41.0 50 39.33357936107974 41.0 39.0 41.0 36.0 41.0 51 38.4334720193802 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 3.0 11 12.0 12 11.0 13 16.0 14 17.0 15 28.0 16 33.0 17 32.0 18 94.0 19 230.0 20 427.0 21 906.0 22 1654.0 23 2897.0 24 4815.0 25 7133.0 26 10877.0 27 15401.0 28 21483.0 29 28468.0 30 38151.0 31 50098.0 32 66538.0 33 89325.0 34 125042.0 35 180113.0 36 290973.0 37 569956.0 38 1664389.0 39 9424098.0 40 15111.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.2952781333302 31.88289896340908 31.583729765910913 21.238093137349804 2 19.07642199199355 31.94293552185404 32.33753959880822 16.643102887344195 3 16.950129969589955 31.10893953158284 33.1159850302189 18.824945468608302 4 17.69455821839745 30.221233035228924 32.861296345734495 19.22291240063913 5 19.45168172099502 30.503895280692912 32.09057596348574 17.95384703482633 6 19.25458986096022 30.648093554628232 30.918930288490138 19.178386295921413 7 19.876250105684065 30.85502811077181 31.009299087413133 18.259422696130986 8 18.963173006296707 30.899049065407773 31.224920851947672 18.91285707634784 9 18.913807327756388 30.107911164155393 31.83371411322067 19.144567394867554 10 19.107068388258117 30.551546302628086 31.025930943771 19.315454365342795 11 19.20891372325638 30.46132819768258 31.114546933797914 19.215211145263126 12 19.015906463137792 30.596429314134603 30.999971923332616 19.38769229939499 13 18.94274850269671 30.865933595985005 30.97486947918734 19.21644842213095 14 19.065754444639555 30.905915087592064 30.92867780945524 19.099652658313143 15 18.951869453965926 30.842020841135714 30.98346696716632 19.222642737732045 16 18.978772294579123 30.719284562099958 30.98940748238427 19.312535660936646 17 19.260149382206002 30.619878335979333 30.936891002764916 19.183081279049745 18 19.111438513605368 30.505743264732676 30.95995077541569 19.42286744624627 19 19.122848108027636 30.500459508632318 30.88873577662749 19.48795660671256 20 19.268683713486652 30.534168907644737 30.87342863854971 19.323718740318903 21 19.31308726844368 30.412260583562052 30.783042569655265 19.491609578339 22 19.173144924529247 30.522294470765672 30.767783008183162 19.53677759652192 23 19.356685474681278 30.554350625396427 30.709356330935716 19.379607568986582 24 19.29580903566922 30.384602449020516 30.664172993128492 19.655415522181773 25 19.271873113771363 30.44272487811146 30.723954875660603 19.56144713245658 26 19.434545665488123 30.454808267180848 30.599585349041803 19.511060718289226 27 19.365491707740713 30.365662161853322 30.5950689561479 19.673777174258063 28 19.338262666707827 30.481472226015967 30.598358262646073 19.581906844630133 29 19.589604184909863 30.40281058217002 30.526464642240754 19.481120590679364 30 19.412298574427226 30.357942849815682 30.58928837526028 19.64047020049681 31 19.38077623895433 30.383229061032168 30.513650732919988 19.72234396709351 32 19.53075767700981 30.516709674158044 30.486569909929084 19.465962738903066 33 19.417927968935132 30.355596386129374 30.503738083448717 19.722737561486774 34 19.368305686386204 30.439243579145842 30.50312415182161 19.689326582646345 35 19.520724904711827 30.450401333784573 30.542998824666196 19.48587493683741 36 19.48262514574889 30.361861778493417 30.53112879046267 19.624384285295026 37 19.50572782512058 30.43404982568592 30.464958095777728 19.595264253415774 38 19.523965510540606 30.44262689057901 30.5301037206008 19.50330387827959 39 19.459278191557537 30.460787722733578 30.4315282623069 19.648405823401983 40 19.41044434424661 30.51456857799497 30.45951617985222 19.6154708979062 41 19.52846692818354 30.460864795099283 30.49885020565582 19.511818071061352 42 19.454380137785403 30.391721264290783 30.451911764169353 19.70198683375446 43 19.426804055354687 30.429753797899608 30.49766188230074 19.645780264444962 44 19.532916890678926 30.470482463102734 30.41805229007364 19.578548356144704 45 19.54293344875931 30.40023152893509 30.41733598288412 19.63949903942148 46 19.4311480166189 30.436489624608992 30.524086094544224 19.608276264227882 47 19.542398579676053 30.538995297207418 30.445418513006363 19.473187610110163 48 19.473364654803532 30.389638613368614 30.493810856867064 19.64318587496079 49 19.521625780900013 30.45917226363408 30.39745310911034 19.621748846355562 50 19.51477457547455 30.48020483375817 30.429086812438822 19.575933778328466 51 19.44932360376307 30.38449326538529 30.442498526866242 19.723684603985397 >>END_MODULE >>Per base GC content pass #Base %GC 1 36.53337127068001 2 35.71952487933775 3 35.77507543819826 4 36.917470619036585 5 37.40552875582135 6 38.43297615688163 7 38.135672801815055 8 37.876030082644554 9 38.05837472262394 10 38.422522753600916 11 38.424124868519506 12 38.40359876253278 13 38.15919692482766 14 38.1654071029527 15 38.17451219169797 16 38.29130795551578 17 38.44323066125575 18 38.534305959851636 19 38.6108047147402 20 38.592402453805555 21 38.80469684678268 22 38.70992252105117 23 38.73629304366786 24 38.95122455785099 25 38.833320246227935 26 38.945606383777346 27 39.03926888199878 28 38.92016951133796 29 39.07072477558923 30 39.05276877492403 31 39.10312020604784 32 38.996720415912876 33 39.140665530421906 34 39.05763226903255 35 39.00659984154923 36 39.10700943104392 37 39.10099207853635 38 39.02726938882019 39 39.107684014959524 40 39.02591524215281 41 39.04028499924489 42 39.15636697153986 43 39.072584319799645 44 39.11146524682363 45 39.182432488180794 46 39.03942428084679 47 39.01558618978622 48 39.11655052976432 49 39.143374627255575 50 39.09070835380302 51 39.17300820774847 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2143.0 1 2861.0 2 3579.0 3 3886.5 4 4194.0 5 4276.0 6 4358.0 7 5323.0 8 6288.0 9 7515.5 10 8743.0 11 11692.5 12 14642.0 13 18862.0 14 23082.0 15 30651.5 16 38221.0 17 48251.0 18 58281.0 19 74694.0 20 91107.0 21 113618.5 22 136130.0 23 172164.5 24 208199.0 25 263114.5 26 388108.0 27 458186.0 28 539189.5 29 620193.0 30 713098.5 31 806004.0 32 892033.5 33 978063.0 34 1049891.5 35 1121720.0 36 1160909.0 37 1200098.0 38 1208822.0 39 1217546.0 40 1177963.0 41 1138380.0 42 1072799.0 43 1007218.0 44 931131.0 45 855044.0 46 767723.5 47 680403.0 48 606204.5 49 532006.0 50 455267.5 51 378529.0 52 317285.0 53 256041.0 54 213978.0 55 171915.0 56 141005.0 57 110095.0 58 86219.5 59 62344.0 60 49779.5 61 37215.0 62 31775.5 63 26336.0 64 21285.5 65 16235.0 66 12665.5 67 9096.0 68 6676.0 69 4256.0 70 3059.0 71 1862.0 72 1423.0 73 984.0 74 771.0 75 495.0 76 432.0 77 340.5 78 249.0 79 211.0 80 173.0 81 127.0 82 81.0 83 60.0 84 39.0 85 29.5 86 20.0 87 15.0 88 10.0 89 6.5 90 3.0 91 2.5 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04043357353953345 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 7.931261973231358E-6 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 3.965630986615678E-5 18 0.0 19 0.002387309853942638 20 0.0 21 0.003061467121667304 22 4.7587571839388135E-5 23 0.0032597486709980876 24 0.011881030435900572 25 0.003997356034508604 26 0.0546622575195105 27 0.05315531774459655 28 0.056875079610042054 29 0.6139351955619197 30 0.04393919133170172 31 0.0 32 0.05584401555352198 33 0.001070720366386233 34 0.0034263051724359463 35 7.931261973231358E-6 36 0.008200924880321222 37 4.6001319444741867E-4 38 0.0033628550766500952 39 0.003537342840061185 40 0.017147388386126193 41 0.006836747820925429 42 2.5380038314340344E-4 43 9.12095126921606E-4 44 0.023769992133774374 45 0.027204228568183553 46 0.004124256226080305 47 0.004013218558455067 48 0.0029266356681223703 49 0.02258030283778967 50 0.0020304030651472275 51 0.006797091511059272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1.2608334E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Duplicate Percentage 47.79565642003441 #Duplication Level Relative count 1 100.0 2 43.26488797826377 3 12.211482784731192 4 2.8488708743485813 5 0.9148812970469021 6 0.49886419313170904 7 0.35811322435526255 8 0.3206984098703844 9 0.2850652532181195 10++ 17.462919246358737 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content pass >>END_MODULE