##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4790109	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.187915556827623	34.0	31.0	34.0	28.0	34.0
2	31.74417847276544	34.0	31.0	34.0	28.0	34.0
3	32.33022213064463	34.0	31.0	34.0	30.0	34.0
4	36.04220363252694	37.0	35.0	37.0	35.0	37.0
5	36.0417318269793	37.0	35.0	37.0	35.0	37.0
6	36.074910195154224	37.0	35.0	37.0	35.0	37.0
7	36.07090861606698	37.0	35.0	37.0	35.0	37.0
8	36.076478217927814	37.0	35.0	37.0	35.0	37.0
9	37.60398395944643	39.0	38.0	39.0	35.0	39.0
10	37.7263060193411	39.0	38.0	39.0	35.0	39.0
11	37.77283168295335	39.0	38.0	39.0	35.0	39.0
12	37.74987020128352	39.0	38.0	39.0	35.0	39.0
13	37.74054974531895	39.0	38.0	39.0	35.0	39.0
14	39.19498721219079	40.0	39.0	41.0	36.0	41.0
15	39.14346834278719	40.0	39.0	41.0	36.0	41.0
16	39.15299046430885	40.0	39.0	41.0	36.0	41.0
17	39.10821507402024	40.0	39.0	41.0	36.0	41.0
18	39.06100758876259	40.0	38.0	41.0	36.0	41.0
19	39.11030876332877	40.0	39.0	41.0	36.0	41.0
20	39.07841345572721	40.0	39.0	41.0	36.0	41.0
21	39.0225575242651	40.0	39.0	41.0	36.0	41.0
22	38.96292401696913	40.0	39.0	41.0	36.0	41.0
23	38.877967077575896	40.0	38.0	41.0	35.0	41.0
24	38.70875694060407	40.0	38.0	41.0	35.0	41.0
25	38.715943207137876	40.0	38.0	41.0	35.0	41.0
26	34.71915628642271	40.0	37.0	41.0	2.0	41.0
27	34.57254584394635	40.0	37.0	41.0	2.0	41.0
28	34.43991086632893	40.0	37.0	41.0	2.0	41.0
29	34.174281002791375	40.0	36.0	41.0	2.0	41.0
30	34.13581006194222	40.0	36.0	41.0	2.0	41.0
31	34.435857722653076	40.0	37.0	41.0	2.0	41.0
32	34.34090330721075	40.0	36.0	41.0	2.0	41.0
33	34.20668715471819	40.0	36.0	41.0	2.0	41.0
34	34.314707243613874	40.0	37.0	41.0	2.0	41.0
35	34.36336438273116	40.0	37.0	41.0	2.0	41.0
36	34.27986336010308	40.0	37.0	41.0	2.0	41.0
37	34.20128769512343	40.0	37.0	41.0	2.0	41.0
38	34.13641443232294	40.0	36.0	41.0	2.0	41.0
39	34.07286890548837	40.0	36.0	41.0	2.0	41.0
40	34.08971695633648	40.0	36.0	41.0	2.0	41.0
41	33.988718837087006	40.0	36.0	41.0	2.0	41.0
42	33.98105074435676	40.0	36.0	41.0	2.0	41.0
43	33.92778431555524	40.0	36.0	41.0	2.0	41.0
44	33.88853239039028	40.0	35.0	41.0	2.0	41.0
45	33.698061985645836	40.0	35.0	41.0	2.0	41.0
46	33.675472520562685	40.0	35.0	41.0	2.0	41.0
47	33.57901396398287	40.0	35.0	41.0	2.0	41.0
48	33.52321377237971	40.0	35.0	41.0	2.0	41.0
49	33.42666920523103	39.0	35.0	41.0	2.0	41.0
50	33.34000374521749	39.0	35.0	41.0	2.0	41.0
51	32.48183517327059	38.0	33.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	1.0
8	6.0
9	11.0
10	29.0
11	88.0
12	358.0
13	1519.0
14	5813.0
15	16925.0
16	38158.0
17	75072.0
18	153414.0
19	211523.0
20	39114.0
21	6073.0
22	7049.0
23	10549.0
24	13105.0
25	17864.0
26	21233.0
27	24242.0
28	28670.0
29	35264.0
30	42391.0
31	49444.0
32	59834.0
33	76279.0
34	101845.0
35	146655.0
36	231162.0
37	419480.0
38	966391.0
39	1990012.0
40	533.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.79514882567917	27.391369116459334	30.49444171643226	21.319040341429236
2	19.797507697112465	31.50868414657874	29.71400644828885	18.979801708019945
3	19.142194885335595	28.970864754852133	32.10979123857098	19.77714912124129
4	19.461582189465833	28.238626720185284	29.647258548813817	22.652532541535066
5	22.39391629710305	29.222383039717887	29.614607934809	18.769092728370065
6	22.435230597049046	29.364759758076488	28.359500796328437	19.840508848546033
7	19.835749040366306	32.21404773878841	29.028316474635545	18.921886746209744
8	19.159835402492927	32.28636342095764	29.170192160554176	19.38360901599525
9	21.92661289750164	29.0553069180515	29.40335767099935	19.61472251344751
10	19.2197922844762	32.076660468477854	28.987837228756174	19.715710018289773
11	22.028162615923772	29.18090172895857	29.081217149755883	19.709718505361778
12	19.27678472452297	29.176956098493793	29.02284687049919	22.523412306484047
13	19.23574181714863	32.143130772180754	28.951616758616556	19.669510652054058
14	19.160415063970415	29.501127845840543	29.014854580431955	22.323602509757094
15	19.11748127599628	32.0464590777494	29.174978935756773	19.66108071049755
16	19.201734240285557	29.38922684222843	29.107876250832703	22.30116266665331
17	22.03781380570767	29.339723120809285	29.071605110465832	19.55085796301721
18	19.233716811037077	29.07785605713774	31.874055475564333	19.81437165626085
19	19.323697518851887	29.170228894958683	29.05527722330542	22.450796362884013
20	19.393775799256343	29.16545322872611	31.709445442681993	19.731325529335553
21	22.068029074195316	29.028683377202945	29.016219786753606	19.887067761848133
22	19.410923388558963	31.886719858241165	28.933612547694654	19.76874420550522
23	19.445851542340602	31.852005655872063	28.986490537234733	19.715652264552602
24	19.40840242999248	29.01754145851867	29.02869570076161	22.54536041072724
25	19.421169627073127	29.129698119541352	31.601500425460138	19.847631827925383
26	19.55230934176001	28.981745337584293	28.816856172822092	22.649089147833614
27	19.596410475826946	28.964551459006554	28.717276580754287	22.721761484412216
28	19.649214417875587	31.764093491294926	28.603361380973162	19.983330709856325
29	22.238203932844254	29.143227526875194	28.752261726840295	19.86630681344025
30	19.69388440108304	28.96653008923661	31.10943350006065	20.230152009619697
31	19.582141815271818	29.3084411221115	28.735271572466697	22.37414549014999
32	19.755462488925403	31.32292003374489	28.64123190482173	20.280385572507985
33	19.96094649868994	29.109546111844576	28.741787577523993	22.1877198119415
34	21.357461993569373	29.35235083264739	28.743240214203507	20.546946959579728
35	20.83298844563504	29.357507139175354	29.62419155188177	20.185312863307836
36	20.31578984609107	29.905904579058905	29.36036576808822	20.417939806761805
37	20.881983550186686	29.666134672234595	29.006292889538514	20.4455888880402
38	20.615016665586992	29.562845741775845	28.95199488187506	20.870142710762106
39	20.11912831260546	30.395261498899444	28.89918101350906	20.58642917498603
40	19.853617380996017	29.87774402547721	29.809272377291755	20.459366216235022
41	19.86658068343403	29.36343188711242	29.47677054938453	21.29321688006903
42	19.832884095761656	29.15294761813539	30.120806364709413	20.893361921393545
43	19.852455171531524	29.101582766536605	29.637486999803368	21.408475062128502
44	20.880122651668554	29.02882243286724	29.209608344474354	20.881446570989855
45	20.379041722955606	29.081683668584922	30.190726419810872	20.348548188648603
46	20.040502947021636	30.16177559175448	29.459374939474692	20.338346521749195
47	20.23035246060461	29.64779490620269	30.070215790710147	20.051636842482555
48	21.031879115153238	29.15880346738667	29.616645350765005	20.19267206669509
49	20.43781751175161	29.32091151136068	28.950097637532284	21.29117333935542
50	20.19657703312658	29.112800451580494	28.95816877370222	21.732453741590703
51	21.165385423963883	28.828538887825804	28.96428038015168	21.041795308058628
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.11418916710841
2	38.77730940513241
3	38.919344006576885
4	42.1141147310009
5	41.163009025473116
6	42.27573944559508
7	38.757635786576046
8	38.54344441848818
9	41.54133541094915
10	38.935502302765975
11	41.73788112128555
12	41.800197031007016
13	38.905252469202686
14	41.484017573727506
15	38.778561986493834
16	41.502896906938865
17	41.588671768724886
18	39.04808846729792
19	41.7744938817359
20	39.125101328591896
21	41.955096836043445
22	39.179667594064185
23	39.16150380689321
24	41.95376284071972
25	39.26880145499851
26	42.20139848959362
27	42.31817196023916
28	39.63254512773191
29	42.104510746284504
30	39.92403641070274
31	41.9562873054218
32	40.035848061433384
33	42.14866631063144
34	41.9044089531491
35	41.018301308942874
36	40.73372965285287
37	41.32757243822688
38	41.4851593763491
39	40.705557487591484
40	40.31298359723104
41	41.159797563503055
42	40.7262460171552
43	41.26093023366003
44	41.76156922265841
45	40.727589911604205
46	40.37884946877083
47	40.281989303087165
48	41.22455118184833
49	41.728990851107035
50	41.929030774717276
51	42.20718073202251
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	189.0
1	475.5
2	762.0
3	1793.0
4	2824.0
5	5206.5
6	7589.0
7	11596.0
8	15603.0
9	21492.5
10	27382.0
11	34480.0
12	41578.0
13	49610.5
14	57643.0
15	66262.0
16	74881.0
17	83219.5
18	91558.0
19	100274.5
20	108991.0
21	116944.0
22	124897.0
23	132985.0
24	141073.0
25	151556.0
26	166251.5
27	170464.0
28	176878.5
29	183293.0
30	193001.5
31	202710.0
32	210999.0
33	219288.0
34	233338.5
35	247389.0
36	263494.5
37	279600.0
38	293649.0
39	307698.0
40	316705.5
41	325713.0
42	336670.0
43	347627.0
44	344647.0
45	341667.0
46	320946.0
47	300225.0
48	273299.5
49	246374.0
50	226015.5
51	205657.0
52	185170.5
53	164684.0
54	151965.0
55	139246.0
56	118721.0
57	98196.0
58	74458.0
59	50720.0
60	43896.0
61	37072.0
62	29761.5
63	22451.0
64	18763.0
65	15075.0
66	12611.5
67	10148.0
68	8299.5
69	6451.0
70	5388.5
71	4326.0
72	3540.0
73	2754.0
74	2286.0
75	1442.0
76	1066.0
77	853.0
78	640.0
79	515.5
80	391.0
81	278.0
82	165.0
83	126.0
84	87.0
85	64.5
86	42.0
87	32.0
88	22.0
89	16.5
90	11.0
91	9.0
92	7.0
93	6.5
94	6.0
95	5.0
96	4.0
97	5.0
98	6.0
99	6.5
100	7.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.6217376264297954
2	2.087635166548402E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.262905499645207E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	8.350540666193608E-5
15	2.087635166548402E-5
16	0.0
17	0.016701081332387215
18	0.0
19	0.0056366149496806855
20	0.0
21	0.003277587211480991
22	4.175270333096804E-5
23	0.05995688198327011
24	0.2433765077162127
25	2.087635166548402E-5
26	11.20953615042998
27	11.344585269353995
28	11.406358393932162
29	11.94321465336175
30	11.370388440012533
31	10.833720067747937
32	11.260516201197092
33	11.733824846156946
34	11.334460238796236
35	11.221393918175975
36	11.4875047728559
37	11.719315781749435
38	11.699838145645538
39	11.576104009324213
40	11.490302203979075
41	11.881378899728587
42	11.620612391075026
43	11.879040748342053
44	11.69585076247743
45	11.68457753257807
46	11.614412114630376
47	11.75927311883717
48	11.855867997993364
49	11.993818929798882
50	12.015968738915962
51	11.521783742290625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4790109.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	36.51676076616338
#Duplication Level	Relative count
1	100.0
2	8.09214236946003
3	1.5952741413601685
4	0.6607890284085849
5	0.41202837889987237
6	0.2754771870454478
7	0.1988897794401401
8	0.14901890937156767
9	0.09439843262979784
10++	7.262148603318788
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCATCTCGTATGCCG	9815	0.20490139159672566	TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGCATCTCGTATGCCG	8979	0.18744876160438104	TruSeq Adapter, Index 2 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1488025	3.202745	3.5019643	1
AAAAA	1535920	3.131264	3.3451025	14
TGCCG	268045	2.5018182	24.855434	47
GGAAG	348985	2.2809954	39.65257	5
GAGCA	351380	2.247895	38.363155	9
CTGAA	485010	2.12719	26.339956	19
CGGAA	328270	2.1000524	38.996037	4
CTCCA	334785	2.074148	35.80666	24
GAAGA	466345	2.067138	27.310621	6
AGAGC	308890	1.9760722	38.253696	8
TCCAG	308950	1.9556028	34.385723	25
AGCAC	311140	1.9482116	37.36059	10
CACGT	296740	1.8783154	37.142067	14
GCACA	294805	1.8459294	37.081768	11
AAGAG	412950	1.8304573	27.09671	7
TCTGA	410195	1.8186882	26.382643	18
CACAC	295290	1.8097154	36.138634	12
TCGGA	276570	1.7886122	39.02567	3
CGTCT	277600	1.7763319	37.238293	16
TCTCG	273260	1.7485609	16.805853	40
GTCTG	266565	1.7427156	37.99579	17
ATCGG	260280	1.683263	38.305023	2
ATGCC	262835	1.6637024	16.58591	46
TGAAC	373600	1.6385604	25.761738	20
CCAGT	256775	1.6253436	33.113327	26
ACGTC	254310	1.6097406	36.857265	15
CTCGT	244550	1.5648485	16.510597	41
CAGTC	246125	1.557931	31.488798	27
ACACG	247875	1.552076	36.580524	13
ACTCC	250435	1.5515606	36.104168	23
GTCAC	231990	1.4684587	27.341125	29
ATCTC	334450	1.4513774	11.650958	39
GATCG	221815	1.434505	37.737232	1
GGAGC	149810	1.4131728	6.3243656	34
TCACG	217815	1.3787332	18.803131	30
AACTC	318760	1.3683611	25.466831	22
GAACT	309290	1.3565055	25.431723	21
GCATC	208725	1.321195	9.510385	37
AGTCA	297995	1.3069668	21.13508	28
TCACC	206995	1.2824298	6.507498	30
GTATG	271995	1.2321042	11.886431	44
CATCT	281190	1.2202506	7.4120584	38
TATGC	265440	1.1768855	11.576866	45
CGGAG	120960	1.1410279	6.4493556	33
AGCAT	257860	1.1309402	6.577876	36
CGTAT	253170	1.1224838	11.465209	43
TCGTA	249640	1.1068329	11.498303	42
CACGG	105285	0.9720805	13.141196	31
GTAGC	147360	0.9529953	5.667349	34
>>END_MODULE