FastQCFastQC Report
Wed 23 Jul 2014
Undetermined_Undetermined_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename Undetermined_Undetermined_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4846604
Filtered Sequences 0
Sequence length 51
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1425985 3.2550354 3.5512393 14
AAAAA 1478390 3.1751297 3.3267493 13
GGAAG 366230 2.3160172 41.9374 5
TGCCG 259030 2.2697458 11.555625 47
GAGCA 358270 2.2141237 40.46152 9
CTGAA 498790 2.1770296 28.558233 19
CGGAA 344700 2.1302605 40.879345 4
GAAGA 475725 2.0989654 29.653759 6
CTCCA 341805 2.0420706 36.13361 24
AGAGC 329515 2.0364165 40.41704 8
AGCAC 329785 1.9917092 39.19977 10
TCCAG 318905 1.9496202 35.853374 25
AAGAG 432175 1.9068168 29.356422 7
TCTGA 421365 1.8616531 28.628483 18
CGTCT 299585 1.8539689 39.276024 16
GCACA 305870 1.8472766 38.882744 11
CACGT 299305 1.8297961 39.010853 14
CACAC 308305 1.8196139 37.74233 12
TCGGA 290725 1.8187268 41.051865 3
GTCTG 280470 1.7760909 40.08498 17
ATCGG 281530 1.7612045 40.869045 2
TCTCG 281350 1.7411222 12.898943 41
TGAAC 384860 1.6797682 27.953001 20
ACGTC 270970 1.6565706 38.624493 15
CCAGT 267445 1.6350205 33.96934 26
CAGTC 260480 1.5924401 32.4603 27
ATGCC 259175 1.5844619 11.919875 47
ACACG 260675 1.5743251 38.339333 13
CTCGT 250955 1.5530242 11.593197 42
GATCG 244180 1.5275491 40.544674 1
ACTCC 253630 1.5152802 36.360966 23
ATCTC 338300 1.4606491 9.078599 40
GTCAC 231910 1.4177779 26.304777 29
GAACT 315200 1.3757287 27.518963 21
TCACC 228835 1.3671457 12.03871 30
AACTC 317805 1.3555357 26.526443 22
AGTCA 306130 1.3361416 22.094097 28
GTATG 264605 1.1962851 8.107712 45
TCACG 187190 1.144383 7.832561 30
TATGC 258800 1.1434168 7.9744887 46
CGTAT 254875 1.1260756 7.9781632 44
TCGTA 247840 1.094994 8.054189 43
CACCG 115855 0.98005825 5.256716 31
CACCC 97605 0.80688715 8.054262 31