##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4846604	
Filtered Sequences	0	
Sequence length	51	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.143255978825586	34.0	31.0	34.0	31.0	34.0
2	32.58071032830411	34.0	31.0	34.0	31.0	34.0
3	32.835684945582514	34.0	31.0	34.0	31.0	34.0
4	36.31237192062731	37.0	37.0	37.0	35.0	37.0
5	36.249550613171614	37.0	37.0	37.0	35.0	37.0
6	36.24254405765357	37.0	37.0	37.0	35.0	37.0
7	36.206262364327685	37.0	37.0	37.0	35.0	37.0
8	36.194395704703744	37.0	37.0	37.0	35.0	37.0
9	38.002910491552434	39.0	38.0	39.0	35.0	39.0
10	37.967529841513766	39.0	38.0	39.0	35.0	39.0
11	37.969695687949745	39.0	38.0	39.0	35.0	39.0
12	37.951977302044895	39.0	38.0	39.0	35.0	39.0
13	37.92943347548098	39.0	38.0	39.0	35.0	39.0
14	39.43417906641434	41.0	39.0	41.0	37.0	41.0
15	39.35790380233252	41.0	39.0	41.0	36.0	41.0
16	39.37097625471361	41.0	39.0	41.0	36.0	41.0
17	39.35064717480529	41.0	39.0	41.0	36.0	41.0
18	39.30241917845981	40.0	39.0	41.0	36.0	41.0
19	39.35701575783786	40.0	39.0	41.0	36.0	41.0
20	39.33022400839846	40.0	39.0	41.0	36.0	41.0
21	39.27399308876896	40.0	39.0	41.0	36.0	41.0
22	39.17730126084161	40.0	39.0	41.0	36.0	41.0
23	39.12172708973129	40.0	39.0	41.0	36.0	41.0
24	38.81582753614696	40.0	39.0	41.0	36.0	41.0
25	38.88741229941625	40.0	38.0	41.0	36.0	41.0
26	34.640759385334555	40.0	38.0	41.0	2.0	41.0
27	34.436621395104694	40.0	37.0	41.0	2.0	41.0
28	34.254201498616354	40.0	37.0	41.0	2.0	41.0
29	34.00486773831739	40.0	37.0	41.0	2.0	41.0
30	33.98373108263023	40.0	36.0	41.0	2.0	41.0
31	34.31151193701817	40.0	37.0	41.0	2.0	41.0
32	34.21071950586431	40.0	37.0	41.0	2.0	41.0
33	34.105095650480216	40.0	37.0	41.0	2.0	41.0
34	34.23243099704453	40.0	37.0	41.0	2.0	41.0
35	34.180279634977396	40.0	37.0	41.0	2.0	41.0
36	34.11549922378639	40.0	37.0	41.0	2.0	41.0
37	34.03043780758651	40.0	37.0	41.0	2.0	41.0
38	34.046221024040754	40.0	37.0	41.0	2.0	41.0
39	33.98677300641852	40.0	37.0	41.0	2.0	41.0
40	33.9722925578405	40.0	36.0	41.0	2.0	41.0
41	33.98378452211074	40.0	36.0	41.0	2.0	41.0
42	33.87663774469711	40.0	36.0	41.0	2.0	41.0
43	33.82683710078232	40.0	36.0	41.0	2.0	41.0
44	33.76859095564647	40.0	36.0	41.0	2.0	41.0
45	33.63344271576551	40.0	35.0	41.0	2.0	41.0
46	33.57975687718658	40.0	35.0	41.0	2.0	41.0
47	33.44170516097457	40.0	35.0	41.0	2.0	41.0
48	33.41162224105786	40.0	35.0	41.0	2.0	41.0
49	33.267722099845585	40.0	35.0	41.0	2.0	41.0
50	33.169617117470295	39.0	35.0	41.0	2.0	41.0
51	32.35310291494828	38.0	33.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	37.0
7	2.0
8	4.0
9	9.0
10	6.0
11	38.0
12	200.0
13	1134.0
14	4452.0
15	13768.0
16	31910.0
17	65728.0
18	154399.0
19	245566.0
20	65030.0
21	11308.0
22	9100.0
23	10976.0
24	12029.0
25	15048.0
26	18468.0
27	19954.0
28	24229.0
29	30758.0
30	37899.0
31	40626.0
32	47726.0
33	61631.0
34	82657.0
35	121390.0
36	197649.0
37	370924.0
38	915485.0
39	2235657.0
40	807.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.50846536410962	27.268431684872933	29.721109529778794	21.501993421238655
2	20.0003548876698	31.57284977274809	29.040169157620472	19.38662618196164
3	19.431482332783943	28.546648333554792	31.98996658278663	20.031902750874632
4	19.821714338534775	27.89169488573855	29.10497742336696	23.181613352359715
5	22.998970000437417	28.690831765912794	29.111579984665553	19.198618248984236
6	23.094996001323814	28.89806965867234	27.878345332112957	20.128589007890888
7	20.210914694082703	31.975936140027123	28.479797400406554	19.333351765483624
8	19.67414709351125	32.03071676580137	28.534763723217328	19.760372417470045
9	22.441507496795694	28.584282932956768	28.953985099669787	20.020224470577748
10	19.55528035713254	31.90941533494381	28.45802958112526	20.077274726798393
11	22.604528861858736	28.787043463835708	28.548484670915965	20.05994300338959
12	19.621553566167155	28.696402677008482	28.61118837024853	23.070855386575836
13	19.52443401606568	31.8925788036324	28.55440634308064	20.02858083722128
14	19.48729005901044	29.0692444187678	28.582552717368877	22.860912804852887
15	19.4530768168923	31.823339326071753	28.742818669694604	19.98076518734134
16	19.522094598107838	28.944620928318937	28.678925349384425	22.8543591241888
17	22.575166751227822	28.92769371296237	28.583781878850516	19.913357656959292
18	19.56343039373549	28.676202966035596	31.615498192136183	20.144868448092726
19	19.553457393579375	28.778269993005363	28.65556465706675	23.01270795634851
20	19.715454074083702	28.743042285941133	31.463506677569157	20.077996962406008
21	22.598205515522206	28.639732890902934	28.60343461357502	20.158626979999834
22	19.700079395848377	31.67448445340108	28.54319972287835	20.082236427872196
23	19.735828411055483	31.613788252862925	28.59462836243292	20.055754973648675
24	19.710397627105905	28.63974875825228	28.589316869020553	23.06053674562126
25	19.735572345071528	28.739227115079757	31.378799280229224	20.146401259619484
26	19.877328614926494	28.662234091208667	28.429378462167765	23.031058831697074
27	19.932253386448558	28.642750700695224	28.345794711294747	23.079201201561467
28	20.058366304551033	31.486945142321794	28.227739388957268	20.22694916416991
29	22.723148829153715	28.73788227478706	28.29969868798785	20.239270208071368
30	20.02308329181874	28.56797617238979	30.876752887472485	20.532187648318985
31	19.907167778265368	28.873095505045832	28.3724391261923	22.847297590496503
32	20.058755140429152	31.091930923150173	28.24665232339415	20.60266161302652
33	20.111924944837362	28.855509751848352	28.539610454570337	22.492954848743953
34	20.74494328318898	29.220931598225352	28.560727884226306	21.47339723435936
35	20.893731967455313	29.100245421403987	28.913168062845944	21.092854548294753
36	20.78739084866949	29.181747114181523	29.00746321596332	21.023398821185662
37	20.71841952902532	29.282856568950642	28.961185353231013	21.037538548793027
38	20.699087674949162	29.408596824258055	28.92578901726098	20.96652648353181
39	20.416505859650773	29.611121570104014	28.727467407163367	21.24490516308185
40	20.325483900874737	29.86819880961961	28.768741761249057	21.037575528256593
41	20.35030548558726	29.201002282099005	29.431349595206935	21.017342637106797
42	20.249342960023412	28.8583456211708	29.261381977618463	21.630929441187323
43	20.163489861758336	28.86920574918331	29.75638749605054	21.210916893007816
44	20.58062827509846	28.66649658951169	29.071206134694012	21.681669000695834
45	21.04927340916031	28.63279527398694	29.287516943112813	21.030414373739937
46	20.543486757651987	29.166317411373132	29.416085961067374	20.874109869907503
47	20.65707930216048	29.647415842453128	29.198154506062107	20.497350349324286
48	20.835286196175247	29.001569205844156	29.70938571815215	20.45375887982844
49	21.077576074026208	29.109793021000318	28.898549457715255	20.91408144725822
50	20.650207369543597	28.87274332447964	28.702277327312693	21.774771978664074
51	20.945623055452938	28.53089196728929	28.612581509778344	21.91090346747943
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.01045878534828
2	39.38698106963144
3	39.46338508365858
4	43.00332769089449
5	42.197588249421656
6	43.2235850092147
7	39.54426645956633
8	39.434519510981296
9	42.461731967373446
10	39.63255508393093
11	42.66447186524832
12	42.692408952742994
13	39.55301485328696
14	42.34820286386333
15	39.43384200423364
16	42.376453722296645
17	42.48852440818712
18	39.70829884182822
19	42.566165349927886
20	39.793451036489714
21	42.75683249552205
22	39.78231582372057
23	39.79158338470416
24	42.77093437272717
25	39.88197360469101
26	42.90838744662357
27	43.011454588010025
28	40.28531546872094
29	42.96241903722508
30	40.555270940137724
31	42.75446536876187
32	40.661416753455676
33	42.60487979358131
34	42.21834051754834
35	41.98658651575007
36	41.810789669855154
37	41.75595807781834
38	41.66561415848097
39	41.66141102273262
40	41.363059429131326
41	41.367648122694064
42	41.88027240121073
43	41.37440675476615
44	42.26229727579429
45	42.07968778290025
46	41.4175966275595
47	41.15442965148477
48	41.289045076003696
49	41.991657521284424
50	42.42497934820767
51	42.85652652293236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	187.0
1	414.5
2	642.0
3	1525.5
4	2409.0
5	4535.5
6	6662.0
7	10584.5
8	14507.0
9	20175.5
10	25844.0
11	32964.5
12	40085.0
13	48048.5
14	56012.0
15	64655.5
16	73299.0
17	82124.0
18	90949.0
19	98874.0
20	106799.0
21	114357.5
22	121916.0
23	130866.5
24	139817.0
25	151427.5
26	163820.0
27	164602.0
28	169016.5
29	173431.0
30	182464.0
31	191497.0
32	200098.5
33	208700.0
34	223601.0
35	238502.0
36	256406.0
37	274310.0
38	290345.5
39	306381.0
40	319416.5
41	332452.0
42	345629.0
43	358806.0
44	358175.0
45	357544.0
46	335929.5
47	314315.0
48	288032.0
49	261749.0
50	244639.5
51	227530.0
52	204014.5
53	180499.0
54	153484.5
55	126470.0
56	109755.0
57	93040.0
58	74957.5
59	56875.0
60	50303.5
61	43732.0
62	35673.5
63	27615.0
64	23925.0
65	20235.0
66	17618.0
67	15001.0
68	12825.0
69	10649.0
70	9089.0
71	7529.0
72	6385.0
73	5241.0
74	4339.0
75	2756.0
76	2075.0
77	1587.5
78	1100.0
79	845.5
80	591.0
81	424.5
82	258.0
83	204.5
84	151.0
85	108.5
86	66.0
87	48.0
88	30.0
89	19.0
90	8.0
91	8.0
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.4886506097878018
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	8.253201623239695E-5
15	0.003775839742632161
16	0.001650640324647939
17	2.682290527552901E-4
18	0.0
19	7.015221379753741E-4
20	1.0316502029049619E-4
21	0.01328765461341591
22	0.05203643623452628
23	0.023047065532896847
24	0.6232611535830036
25	0.0012586132475440536
26	12.029412759944902
27	12.286314293472294
28	12.47828376322885
29	13.03395119551752
30	12.402684436359975
31	11.464460475830087
32	12.116360239045731
33	12.549570792249584
34	11.633609843098384
35	12.29636256644859
36	12.452575040172459
37	12.68834837754436
38	12.110294135852651
39	12.322112555513097
40	12.315221132157692
41	12.155315350707422
42	12.432829255288858
43	12.624221000931787
44	12.733679087460004
45	12.365421231031046
46	12.368908208716867
47	12.780969932761167
48	12.48230719902018
49	12.669675508871778
50	12.77942245745681
51	12.481626309886263
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4846604.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	38.24531976084976
#Duplication Level	Relative count
1	100.0
2	6.328213796520269
3	1.2619123947192026
4	0.6277504152944948
5	0.4284090577915076
6	0.2611255209395856
7	0.19001544604085915
8	0.13872293299915484
9	0.09733920088596158
10++	7.24040451140967
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1425985	3.2550354	3.5512393	14
AAAAA	1478390	3.1751297	3.3267493	13
GGAAG	366230	2.3160172	41.9374	5
TGCCG	259030	2.2697458	11.555625	47
GAGCA	358270	2.2141237	40.46152	9
CTGAA	498790	2.1770296	28.558233	19
CGGAA	344700	2.1302605	40.879345	4
GAAGA	475725	2.0989654	29.653759	6
CTCCA	341805	2.0420706	36.13361	24
AGAGC	329515	2.0364165	40.41704	8
AGCAC	329785	1.9917092	39.19977	10
TCCAG	318905	1.9496202	35.853374	25
AAGAG	432175	1.9068168	29.356422	7
TCTGA	421365	1.8616531	28.628483	18
CGTCT	299585	1.8539689	39.276024	16
GCACA	305870	1.8472766	38.882744	11
CACGT	299305	1.8297961	39.010853	14
CACAC	308305	1.8196139	37.74233	12
TCGGA	290725	1.8187268	41.051865	3
GTCTG	280470	1.7760909	40.08498	17
ATCGG	281530	1.7612045	40.869045	2
TCTCG	281350	1.7411222	12.898943	41
TGAAC	384860	1.6797682	27.953001	20
ACGTC	270970	1.6565706	38.624493	15
CCAGT	267445	1.6350205	33.96934	26
CAGTC	260480	1.5924401	32.4603	27
ATGCC	259175	1.5844619	11.919875	47
ACACG	260675	1.5743251	38.339333	13
CTCGT	250955	1.5530242	11.593197	42
GATCG	244180	1.5275491	40.544674	1
ACTCC	253630	1.5152802	36.360966	23
ATCTC	338300	1.4606491	9.078599	40
GTCAC	231910	1.4177779	26.304777	29
GAACT	315200	1.3757287	27.518963	21
TCACC	228835	1.3671457	12.03871	30
AACTC	317805	1.3555357	26.526443	22
AGTCA	306130	1.3361416	22.094097	28
GTATG	264605	1.1962851	8.107712	45
TCACG	187190	1.144383	7.832561	30
TATGC	258800	1.1434168	7.9744887	46
CGTAT	254875	1.1260756	7.9781632	44
TCGTA	247840	1.094994	8.054189	43
CACCG	115855	0.98005825	5.256716	31
CACCC	97605	0.80688715	8.054262	31
>>END_MODULE