FastQCFastQC Report
Wed 23 Jul 2014
Undetermined_Undetermined_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename Undetermined_Undetermined_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 4736347
Filtered Sequences 0
Sequence length 51
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGCATCTCGTATGCCG 21908 0.46255056903558794 TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCATCTCGTATGCCG 19803 0.4181070348097384 TruSeq Adapter, Index 3 (97% over 37bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1495280 3.4931877 3.7861347 14
AAAAA 1574145 3.332356 3.6048977 47
TGCCG 318910 2.8634665 34.714283 47
GAGCA 396975 2.4656546 47.422752 9
GGAAG 388030 2.4463644 48.85872 5
CTGAA 514685 2.2661366 33.363853 19
CGGAA 364650 2.2648807 47.92089 4
CTCCA 354925 2.1821814 45.185955 24
AGAGC 351200 2.1813412 47.41317 8
GAAGA 487725 2.1372128 34.251865 6
AGCAC 345985 2.1170914 46.383522 10
TCCAG 333890 2.0837438 43.50223 25
CACGT 329905 2.0588741 46.5961 14
CGTCT 316975 2.017551 47.228207 16
GCACA 325035 1.9888979 46.077843 11
AAGAG 452220 1.9816296 34.06842 7
CACAC 328535 1.9805112 45.183525 12
TCTGA 437355 1.9639808 33.746445 18
TCTCG 308180 1.9615705 24.082115 40
ATGCC 312810 1.9521877 23.827917 46
GTCTG 299785 1.9368503 47.9013 17
TCGGA 304760 1.9305702 48.535454 3
ATCGG 301990 1.9130231 48.454506 2
CCAGT 288580 1.8009728 41.92416 26
TGAAC 403615 1.7771002 32.783783 20
CTCGT 278760 1.7743119 23.75286 41
ACGTC 284245 1.7739187 46.258858 15
CAGTC 279415 1.7437756 40.29722 27
ACTCC 276660 1.7009852 45.55068 23
ACACG 274885 1.6820287 45.51559 13
GATCG 263345 1.6682174 48.349434 1
ATCTC 368780 1.6314876 16.996443 39
GTCAC 255665 1.5955565 34.402885 29
GCATC 253425 1.5815772 18.832151 37
GCCGT 171000 1.5353949 5.905203 47
TCACG 241475 1.5069995 23.317936 30
GAACT 333965 1.470434 32.417736 21
AACTC 338100 1.4665707 32.50812 22
AGTCA 328610 1.446856 27.402578 28
GTATG 313965 1.4311036 17.180798 44
GGAGC 156830 1.4014618 12.037699 34
TATGC 308420 1.3849869 17.029274 45
CATCT 306500 1.35596 14.151691 38
AGCAT 297055 1.3079208 12.954967 36
CGTAT 289550 1.3002496 16.767775 43
TCGTA 277445 1.245891 16.753363 42
CGGAG 132680 1.185653 12.102124 33
GTAGC 175040 1.10883 10.525656 34
CACGG 121525 1.0698701 18.608381 31
ACGGA 155495 0.96579623 9.076279 32
TAGCA 203075 0.89413077 7.387507 35
CGTAG 83485 0.52885437 7.4229946 33
ACGTA 114685 0.50495327 5.3768034 32
Produced by FastQC (version 0.10.1)