##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Undetermined_Undetermined_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4736347	
Filtered Sequences	0	
Sequence length	51	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33802147520019	34.0	31.0	34.0	31.0	34.0
2	32.728361118811605	34.0	31.0	34.0	31.0	34.0
3	32.940094549660316	34.0	31.0	34.0	31.0	34.0
4	36.34323540906103	37.0	37.0	37.0	35.0	37.0
5	36.305043950538256	37.0	37.0	37.0	35.0	37.0
6	36.32064985948031	37.0	37.0	37.0	35.0	37.0
7	36.2937348129265	37.0	37.0	37.0	35.0	37.0
8	36.29197290654591	37.0	37.0	37.0	35.0	37.0
9	38.08418534368364	39.0	38.0	39.0	37.0	39.0
10	38.04568943111643	39.0	38.0	39.0	37.0	39.0
11	38.044849332196314	39.0	38.0	39.0	37.0	39.0
12	38.02675796346847	39.0	38.0	39.0	35.0	39.0
13	38.01495202948602	39.0	38.0	39.0	35.0	39.0
14	39.54912340670985	41.0	39.0	41.0	37.0	41.0
15	39.49727648755465	41.0	39.0	41.0	37.0	41.0
16	39.48473517670897	41.0	39.0	41.0	37.0	41.0
17	39.454880311767695	41.0	39.0	41.0	37.0	41.0
18	39.38018603788954	41.0	39.0	41.0	37.0	41.0
19	39.44816057607266	41.0	39.0	41.0	37.0	41.0
20	39.4327852245623	41.0	39.0	41.0	37.0	41.0
21	39.393545489804694	41.0	39.0	41.0	37.0	41.0
22	39.31944471129332	40.0	39.0	41.0	36.0	41.0
23	39.23907961135449	40.0	39.0	41.0	36.0	41.0
24	38.980205842181746	40.0	39.0	41.0	36.0	41.0
25	39.043044988046695	40.0	39.0	41.0	36.0	41.0
26	35.384920488300374	40.0	38.0	41.0	2.0	41.0
27	35.188719492047355	40.0	38.0	41.0	2.0	41.0
28	35.03528267671266	40.0	38.0	41.0	2.0	41.0
29	34.80464607006201	40.0	38.0	41.0	2.0	41.0
30	34.79416182978147	40.0	37.0	41.0	2.0	41.0
31	35.11900775006561	40.0	38.0	41.0	9.0	41.0
32	35.00928964875251	40.0	38.0	41.0	2.0	41.0
33	34.98516388262938	40.0	38.0	41.0	2.0	41.0
34	35.07893129451875	40.0	38.0	41.0	2.0	41.0
35	34.99669534347885	40.0	38.0	41.0	2.0	41.0
36	34.8915641104843	40.0	38.0	41.0	2.0	41.0
37	34.80897598930146	40.0	38.0	41.0	2.0	41.0
38	34.824431149153554	40.0	37.0	41.0	2.0	41.0
39	34.751478301737606	40.0	37.0	41.0	2.0	41.0
40	34.81145849322273	40.0	37.0	41.0	2.0	41.0
41	34.76378356568892	40.0	37.0	41.0	2.0	41.0
42	34.73117151256021	40.0	37.0	41.0	2.0	41.0
43	34.663598760817145	40.0	37.0	41.0	2.0	41.0
44	34.574644974280815	40.0	37.0	41.0	2.0	41.0
45	34.43069648402028	40.0	36.0	41.0	2.0	41.0
46	34.37400954786463	40.0	36.0	41.0	2.0	41.0
47	34.307720696984404	40.0	36.0	41.0	2.0	41.0
48	34.20504093133379	40.0	36.0	41.0	2.0	41.0
49	34.1260237056111	40.0	35.0	41.0	2.0	41.0
50	34.0225308660873	40.0	35.0	41.0	2.0	41.0
51	33.182894538765844	38.0	34.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	0.0
9	5.0
10	5.0
11	36.0
12	196.0
13	930.0
14	3832.0
15	11762.0
16	27099.0
17	54433.0
18	128524.0
19	193790.0
20	67454.0
21	11966.0
22	8118.0
23	9574.0
24	9569.0
25	13765.0
26	13779.0
27	17048.0
28	20583.0
29	24140.0
30	29804.0
31	35464.0
32	43546.0
33	55869.0
34	75709.0
35	113293.0
36	183950.0
37	351490.0
38	885349.0
39	2344149.0
40	1113.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.571846863835535	27.055075738354674	28.974592164903214	21.398485232906584
2	19.931436611380036	32.15558319523464	28.324466091694717	19.588514101690606
3	19.335323193169756	28.527280623653628	32.34081033336451	19.7965858498121
4	19.762424501414273	28.073534308191523	28.78562318174745	23.378418008646747
5	23.4260285405609	28.874805836650058	28.87649490208382	18.82267072070522
6	23.538815884900323	28.8752281030085	27.784260739341942	19.80169527274923
7	19.975753465698354	32.4779202199501	28.434318684842978	19.112007629508565
8	19.46827375612471	32.548839854850165	28.49229585585685	19.490590533168284
9	22.975639242648395	28.480662417681813	28.77388417698281	19.769814162686984
10	19.51491307541445	32.420576448473895	28.230532940259657	19.833977535851997
11	23.079073387148366	28.761680679223883	28.35193451831126	19.807311415316487
12	19.53921450434269	28.664538303464678	28.450575939642935	23.345671252549696
13	19.386100722772213	32.50462856712145	28.359408632855658	19.749862077250675
14	19.36716170833188	29.03380831572868	28.42563236848245	23.17339760745698
15	19.330225145830887	32.33759290992055	28.557771158463513	19.774410785785047
16	19.41800294615238	28.916040146551765	28.546113703240074	23.119843204055783
17	22.996351407529897	28.898410526086877	28.46622090822315	19.639017158160076
18	19.44021415660635	28.652440372295356	32.077759505374075	19.829585965724217
19	19.526173339506943	28.70291487702714	28.482679160836682	23.288232622629234
20	19.60570034247913	28.66574176258623	31.91210441295792	19.816453481976723
21	23.03908640235452	28.558942965395683	28.448949572601023	19.95302105964877
22	19.552742207977175	32.21483340748425	28.408093437337477	19.824330947201098
23	19.611036922908564	32.16570890999961	28.404250846685624	19.819003320406207
24	19.548282521448982	28.581995356496797	28.501129887983286	23.368592234070935
25	19.58186340654517	28.71958072328738	31.840783625017337	19.857772245150112
26	19.682209265478836	28.620188443553808	28.266721410817023	23.430880880150333
27	19.684496772044515	28.609999662414427	28.225671470690372	23.479832094850686
28	19.85774930703868	32.130678989762785	28.046978296080184	19.964593407118354
29	23.098244548069083	28.700017771247644	28.25852002857674	19.943217652106533
30	19.85340093992533	28.520863673031656	31.29819066145562	20.327544725587394
31	19.755094137357194	28.946009580710612	28.172943893629977	23.125952388302213
32	19.857201171271292	31.665498698718537	28.103456005360854	20.373844124649317
33	20.117565618717666	28.789760012955096	28.401624941950388	22.69104942637685
34	21.872826860184205	29.186620619384467	28.547890660399727	20.3926618600316
35	21.30158377142965	29.00897170788952	29.456894634483344	20.232549886197482
36	20.37824752948677	30.423056415744494	28.78662602273935	20.41207003202939
37	21.52852596046873	29.472979716803522	28.533177843230078	20.46531647949767
38	20.507056446386514	29.316479369856957	28.494158158852645	21.682306024903887
39	20.16391501895334	30.644555620535428	28.601264818765344	20.59026454174589
40	19.96359266247765	29.775006806322494	29.865647676437103	20.39575285476275
41	19.92479255381938	29.111186232343982	29.254967956641227	21.709053257195414
42	19.832026666546877	28.762383505854643	30.27621929494607	21.129370532652406
43	19.991521702639368	28.754373103881903	29.30131337740657	21.95279181607216
44	21.57438626285409	28.74474448554824	28.75465618840013	20.926213063197537
45	20.697104956869474	28.808440734937502	30.185109242661646	20.309345065531375
46	20.193267329738426	30.433888570550355	29.199451846342416	20.173392253368803
47	20.30763535216021	29.55425842146977	30.171387290909024	19.966718935460996
48	21.647550016039038	28.93677674441465	29.221799131398786	20.193874108147522
49	20.683120373788842	28.989064607272102	28.441440791615374	21.886374227323678
50	20.41006196319907	28.714640488336762	28.398355403019558	22.47694214544461
51	21.742392864640365	28.514227976707197	28.46637900546008	21.277000153192358
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.97033209674212
2	39.51995071307064
3	39.13190904298186
4	43.14084251006102
5	42.24869926126612
6	43.34051115764956
7	39.08776109520692
8	38.958864289293
9	42.745453405335375
10	39.34889061126645
11	42.88638480246485
12	42.88488575689239
13	39.13596280002289
14	42.54055931578886
15	39.10463593161593
16	42.53784615020817
17	42.63536856568997
18	39.26980012233056
19	42.81440596213618
20	39.422153824455854
21	42.99210746200329
22	39.37707315517827
23	39.43004024331477
24	42.91687475551992
25	39.43963565169528
26	43.113090145629165
27	43.164328866895204
28	39.82234271415703
29	43.041462200175616
30	40.18094566551272
31	42.881046525659414
32	40.23104529592061
33	42.80861504509452
34	42.26548872021581
35	41.53413365762714
36	40.79031756151616
37	41.9938424399664
38	42.1893624712904
39	40.75417956069923
40	40.3593455172404
41	41.633845811014794
42	40.96139719919928
43	41.94431351871153
44	42.50059932605163
45	41.00645002240085
46	40.36665958310723
47	40.27435428762121
48	41.84142412418656
49	42.569494601112524
50	42.88700410864368
51	43.019393017832726
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	316.0
1	481.5
2	647.0
3	1633.0
4	2619.0
5	4632.0
6	6645.0
7	10222.0
8	13799.0
9	18830.5
10	23862.0
11	30296.5
12	36731.0
13	43887.0
14	51043.0
15	58779.5
16	66516.0
17	74834.0
18	83152.0
19	91201.5
20	99251.0
21	107471.5
22	115692.0
23	124723.0
24	133754.0
25	144788.0
26	159889.5
27	163957.0
28	167922.5
29	171888.0
30	180689.0
31	189490.0
32	197133.0
33	204776.0
34	219695.0
35	234614.0
36	251719.0
37	268824.0
38	283772.0
39	298720.0
40	311046.0
41	323372.0
42	336296.5
43	349221.0
44	347598.5
45	345976.0
46	325556.5
47	305137.0
48	277310.5
49	249484.0
50	232289.0
51	215094.0
52	192983.5
53	170873.0
54	161937.0
55	153001.0
56	130707.5
57	108414.0
58	80175.0
59	51936.0
60	47125.5
61	42315.0
62	35244.0
63	28173.0
64	24575.5
65	20978.0
66	18451.0
67	15924.0
68	13684.5
69	11445.0
70	9969.0
71	8493.0
72	7196.5
73	5900.0
74	4817.5
75	3029.5
76	2324.0
77	1770.5
78	1217.0
79	926.5
80	636.0
81	466.5
82	297.0
83	223.0
84	149.0
85	114.0
86	79.0
87	52.0
88	25.0
89	18.0
90	11.0
91	8.0
92	5.0
93	6.0
94	7.0
95	4.5
96	2.0
97	2.0
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.4727594916504219
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.008065287446211183
15	8.234193989587334E-4
16	0.0
17	0.0
18	0.0
19	0.0012245724394770907
20	0.0
21	0.012836897296587433
22	0.01076779214022959
23	0.06101748879463434
24	0.5239903241886626
25	0.0
26	10.318268488351888
27	10.564766475091458
28	10.72041385481258
29	11.251878293545637
30	10.620674540948963
31	9.717510140198765
32	10.519013915154444
33	10.299625428626745
34	10.070144776132322
35	10.76202820443688
36	10.983549136074702
37	11.069480340017316
38	10.408717942329817
39	10.827268356815917
40	10.50685264403136
41	10.748916834007307
42	10.696598032196542
43	10.94765649560727
44	10.96013446649918
45	10.93156814735069
46	10.97915756594692
47	10.918351210331506
48	11.079171352943524
49	11.016401458761361
50	11.06354749768123
51	10.691171909490583
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4736347.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	42.40042492770326
#Duplication Level	Relative count
1	100.0
2	9.586836633215803
3	1.8473314649067294
4	0.6931259982521171
5	0.39176686857728354
6	0.2428954585179158
7	0.17840460476750142
8	0.13741976313172408
9	0.09342133019919839
10++	7.372449748365127
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGCATCTCGTATGCCG	21908	0.46255056903558794	TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCATCTCGTATGCCG	19803	0.4181070348097384	TruSeq Adapter, Index 3 (97% over 37bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1495280	3.4931877	3.7861347	14
AAAAA	1574145	3.332356	3.6048977	47
TGCCG	318910	2.8634665	34.714283	47
GAGCA	396975	2.4656546	47.422752	9
GGAAG	388030	2.4463644	48.85872	5
CTGAA	514685	2.2661366	33.363853	19
CGGAA	364650	2.2648807	47.92089	4
CTCCA	354925	2.1821814	45.185955	24
AGAGC	351200	2.1813412	47.41317	8
GAAGA	487725	2.1372128	34.251865	6
AGCAC	345985	2.1170914	46.383522	10
TCCAG	333890	2.0837438	43.50223	25
CACGT	329905	2.0588741	46.5961	14
CGTCT	316975	2.017551	47.228207	16
GCACA	325035	1.9888979	46.077843	11
AAGAG	452220	1.9816296	34.06842	7
CACAC	328535	1.9805112	45.183525	12
TCTGA	437355	1.9639808	33.746445	18
TCTCG	308180	1.9615705	24.082115	40
ATGCC	312810	1.9521877	23.827917	46
GTCTG	299785	1.9368503	47.9013	17
TCGGA	304760	1.9305702	48.535454	3
ATCGG	301990	1.9130231	48.454506	2
CCAGT	288580	1.8009728	41.92416	26
TGAAC	403615	1.7771002	32.783783	20
CTCGT	278760	1.7743119	23.75286	41
ACGTC	284245	1.7739187	46.258858	15
CAGTC	279415	1.7437756	40.29722	27
ACTCC	276660	1.7009852	45.55068	23
ACACG	274885	1.6820287	45.51559	13
GATCG	263345	1.6682174	48.349434	1
ATCTC	368780	1.6314876	16.996443	39
GTCAC	255665	1.5955565	34.402885	29
GCATC	253425	1.5815772	18.832151	37
GCCGT	171000	1.5353949	5.905203	47
TCACG	241475	1.5069995	23.317936	30
GAACT	333965	1.470434	32.417736	21
AACTC	338100	1.4665707	32.50812	22
AGTCA	328610	1.446856	27.402578	28
GTATG	313965	1.4311036	17.180798	44
GGAGC	156830	1.4014618	12.037699	34
TATGC	308420	1.3849869	17.029274	45
CATCT	306500	1.35596	14.151691	38
AGCAT	297055	1.3079208	12.954967	36
CGTAT	289550	1.3002496	16.767775	43
TCGTA	277445	1.245891	16.753363	42
CGGAG	132680	1.185653	12.102124	33
GTAGC	175040	1.10883	10.525656	34
CACGG	121525	1.0698701	18.608381	31
ACGGA	155495	0.96579623	9.076279	32
TAGCA	203075	0.89413077	7.387507	35
CGTAG	83485	0.52885437	7.4229946	33
ACGTA	114685	0.50495327	5.3768034	32
>>END_MODULE