##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW244_ATGTCA_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7106634	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.776198971271068	34.0	31.0	34.0	30.0	34.0
2	32.09226308826373	34.0	31.0	34.0	30.0	34.0
3	32.480093388797	34.0	31.0	34.0	30.0	34.0
4	36.11118315647042	37.0	35.0	37.0	35.0	37.0
5	36.08264024853398	37.0	35.0	37.0	35.0	37.0
6	36.09720931175012	37.0	36.0	37.0	35.0	37.0
7	36.09672061344372	37.0	36.0	37.0	35.0	37.0
8	36.090240471086595	37.0	35.0	37.0	35.0	37.0
9	37.6241707396216	39.0	38.0	39.0	35.0	39.0
10	37.740757298040116	39.0	38.0	39.0	35.0	39.0
11	37.77801839802078	39.0	38.0	39.0	35.0	39.0
12	37.755771438349015	39.0	38.0	39.0	35.0	39.0
13	37.7438026216068	39.0	38.0	39.0	35.0	39.0
14	39.200441728109254	40.0	39.0	41.0	36.0	41.0
15	39.153430864738496	40.0	39.0	41.0	36.0	41.0
16	39.14618284830765	40.0	39.0	41.0	36.0	41.0
17	39.1171606698755	40.0	39.0	41.0	36.0	41.0
18	39.09054469387336	40.0	38.0	41.0	36.0	41.0
19	39.12564471450197	40.0	39.0	41.0	36.0	41.0
20	39.08656883132014	40.0	39.0	41.0	36.0	41.0
21	39.03929694423549	40.0	39.0	41.0	36.0	41.0
22	38.98048991970038	40.0	38.0	41.0	36.0	41.0
23	38.90849001088279	40.0	38.0	41.0	35.0	41.0
24	38.82471476651253	40.0	38.0	41.0	35.0	41.0
25	38.80293286526364	40.0	38.0	41.0	35.0	41.0
26	38.69615221495859	40.0	38.0	41.0	35.0	41.0
27	38.590620116358885	40.0	38.0	41.0	34.0	41.0
28	38.50069343658334	40.0	38.0	41.0	34.0	41.0
29	38.20144487530946	40.0	38.0	41.0	34.0	41.0
30	38.18888632790151	40.0	38.0	41.0	34.0	41.0
31	38.44117946696003	40.0	38.0	41.0	34.0	41.0
32	38.411831817988656	40.0	38.0	41.0	34.0	41.0
33	38.43314767581953	40.0	38.0	41.0	34.0	41.0
34	38.57375629587791	40.0	38.0	41.0	35.0	41.0
35	38.63275173591323	40.0	38.0	41.0	35.0	41.0
36	38.64122818200571	40.0	38.0	41.0	35.0	41.0
37	38.59060210501906	40.0	38.0	41.0	34.0	41.0
38	38.54166585756351	40.0	38.0	41.0	34.0	41.0
39	38.469113507182165	40.0	38.0	41.0	34.0	41.0
40	38.429005630513686	40.0	38.0	41.0	34.0	41.0
41	38.39976703457643	40.0	38.0	41.0	34.0	41.0
42	38.34717068586901	40.0	38.0	41.0	34.0	41.0
43	38.31228581632317	40.0	38.0	41.0	34.0	41.0
44	38.244470307602725	40.0	38.0	41.0	33.0	41.0
45	38.143444139658804	40.0	38.0	41.0	33.0	41.0
46	38.113749631682175	40.0	38.0	41.0	33.0	41.0
47	38.00945736054509	40.0	38.0	41.0	33.0	41.0
48	37.91372300867049	40.0	37.0	41.0	33.0	41.0
49	37.86913227837539	40.0	37.0	41.0	33.0	41.0
50	37.78763715705635	40.0	37.0	41.0	33.0	41.0
51	36.73685882796272	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	0.0
12	3.0
13	5.0
14	12.0
15	26.0
16	53.0
17	135.0
18	288.0
19	679.0
20	1262.0
21	1994.0
22	3491.0
23	5731.0
24	9087.0
25	14182.0
26	20859.0
27	29829.0
28	40787.0
29	54811.0
30	72213.0
31	94339.0
32	121147.0
33	158026.0
34	210362.0
35	295125.0
36	439293.0
37	732233.0
38	1547277.0
39	3252446.0
40	934.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.57587710894496	28.29732834943209	30.436735205867492	22.690059335755457
2	19.7603168420709	30.52938438348855	32.090236275073764	17.62006249936679
3	18.43892622020495	30.604010281097914	31.251503876518754	19.705559622178377
4	19.128000682179497	29.459488134607746	30.458878281898293	20.95363290131446
5	20.5531760887081	30.274641975371182	30.04518032024725	19.127001615673468
6	20.476107254151543	30.477635403764992	29.37414534081817	19.672112001265297
7	19.389418394137085	31.77220045382948	29.81704137289186	19.021339779141574
8	18.338555214747235	31.4512608922874	30.72647332056217	19.483710572403194
9	19.79745674109951	29.748361741078398	30.9161585730508	19.538022944771292
10	18.805611770635718	31.095297717597386	30.502035140686857	19.597055371080035
11	20.01185089875179	29.9376610642957	30.402916486201487	19.64757155075103
12	18.86063078526346	29.956840326939588	30.27361476614667	20.908914121650277
13	18.948576780512404	31.14762910261032	30.203187613151318	19.700606503725957
14	18.99232463638904	30.098778127591768	30.22368958356375	20.685207652455436
15	18.947549571287897	31.25206673088835	30.14645189269632	19.653931805127435
16	19.047653220920058	30.10367496060723	30.101592399439735	20.74707941903298
17	20.290157096898067	30.085133142516295	30.085836710577286	19.538873050008352
18	19.152161205994286	29.963777507044824	31.13856714725987	19.745494139701016
19	19.139267267077958	29.963893989409524	29.957758824119583	20.939079919392935
20	19.226711267246912	29.89311114094239	31.13554180502331	19.744635786787388
21	20.4263229365286	29.838806138172195	29.901454333096456	19.83341659220275
22	19.244251772922905	31.018066801320344	29.93116564738316	19.80651577837359
23	19.224924033543726	31.015970615890463	30.005927029986662	19.75317832057915
24	19.30269402032132	29.74981543854139	29.945466064474218	21.002024476663074
25	19.27064205079367	29.840962683599574	31.03279273985406	19.855602525752698
26	19.291652771253364	29.84658759853895	29.952054313387332	20.90970531682035
27	19.33747643411427	29.865756271203665	29.831268750829636	20.96549854385243
28	19.33967597102603	31.014445168914158	29.78047347467016	19.86540538538965
29	20.521961811050215	29.884968112151878	29.85792111083592	19.735148965961987
30	19.444221902920113	29.757592440190106	30.88489002498488	19.913295631904898
31	19.42631013815222	29.781619013978467	29.757090204941505	21.03498064292781
32	19.478043383099823	30.931599196179434	29.73350204476224	19.85685537595851
33	19.44763936300448	29.831494575786056	29.707708001632284	21.01315805957718
34	19.47121795652171	30.989186370618903	29.66386761589977	19.875728056959616
35	19.493615592438175	29.893345056914626	30.83056544795273	19.782473902694463
36	20.595012727774527	29.825720899263324	29.69580428881035	19.883462084151795
37	19.467302593849794	29.903681862983987	30.77754682671266	19.85146871645356
38	19.502177076182395	29.92608793633898	29.697534702100274	20.87420028537835
39	19.538709301481084	30.98053091964254	29.62784054282145	19.852919236054927
40	20.626747734227003	29.856093537933486	29.65024341945424	19.866915308385266
41	19.558297951277265	30.942014431684683	29.665924050476132	19.83376356656192
42	19.55828969309777	30.869461555870576	29.69413083400797	19.878117917023687
43	19.499745249106965	29.79273692536784	30.774231595303576	19.93328623022162
44	19.50637938791821	29.82736620131195	29.754325414858297	20.91192899591155
45	19.5157941812163	29.8008089563568	30.810618287457153	19.87277857496975
46	19.525612638317817	29.83792480690755	29.665897628274333	20.970564926500295
47	20.707961886704933	29.911595890087078	29.660003895545344	19.720438327662652
48	19.597248865996704	29.81384064549842	30.75404551831737	19.83486497018751
49	19.620828401700656	30.906622907076077	29.628343717322803	19.84420497390046
50	19.640877236389553	29.82774582516691	30.745579998294236	19.785796940149304
51	20.726389661969076	29.746665043491475	29.62221066609058	19.904734628448868
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.265936444700415
2	37.38037934143769
3	38.14448584238333
4	40.08163358349396
5	39.68017770438157
6	40.14821925541684
7	38.41075817327866
8	37.82226578715043
9	39.3354796858708
10	38.40266714171576
11	39.65942244950282
12	39.76954490691374
13	38.64918328423836
14	39.67753228884448
15	38.60148137641533
16	39.79473263995303
17	39.82903014690642
18	38.897655345695306
19	40.07834718647089
20	38.9713470540343
21	40.259739528731345
22	39.050767551296495
23	38.978102354122875
24	40.30471849698439
25	39.12624457654636
26	40.20135808807372
27	40.3029749779667
28	39.205081356415675
29	40.2571107770122
30	39.35751753482501
31	40.46129078108003
32	39.33489875905833
33	40.46079742258166
34	39.34694601348133
35	39.27608949513264
36	40.47847481192632
37	39.318771310303354
38	40.37637736156074
39	39.39162853753601
40	40.49366304261227
41	39.39206151783918
42	39.43640761012146
43	39.433031479328584
44	40.41830838382975
45	39.38857275618604
46	40.49617756481811
47	40.428400214367585
48	39.432113836184215
49	39.465033375601124
50	39.42667417653885
51	40.631124290417944
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	14.5
2	16.0
3	37.5
4	59.0
5	105.5
6	152.0
7	371.5
8	591.0
9	1245.5
10	1900.0
11	3432.5
12	4965.0
13	8303.0
14	11641.0
15	18014.0
16	24387.0
17	34994.5
18	45602.0
19	61664.5
20	77727.0
21	99410.0
22	121093.0
23	147121.0
24	173149.0
25	201204.5
26	258919.0
27	288578.0
28	317299.5
29	346021.0
30	372984.5
31	399948.0
32	420396.5
33	440845.0
34	460830.0
35	480815.0
36	497148.5
37	513482.0
38	522736.5
39	531991.0
40	533550.5
41	535110.0
42	529163.0
43	523216.0
44	506605.5
45	489995.0
46	460865.5
47	431736.0
48	430468.0
49	429200.0
50	360659.0
51	292118.0
52	256597.5
53	221077.0
54	190488.0
55	159899.0
56	136672.0
57	113445.0
58	96805.0
59	80165.0
60	67194.5
61	54224.0
62	44308.0
63	34392.0
64	27436.0
65	20480.0
66	16596.0
67	12712.0
68	10008.5
69	7305.0
70	5708.0
71	4111.0
72	3232.0
73	2353.0
74	1849.5
75	1013.0
76	680.0
77	533.5
78	387.0
79	305.0
80	223.0
81	163.5
82	104.0
83	77.0
84	50.0
85	40.5
86	31.0
87	27.5
88	24.0
89	15.5
90	7.0
91	6.0
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04920754326169041
2	2.8142718479662805E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	5.628543695932561E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.4071359239831402E-5
18	0.0
19	8.583529136297156E-4
20	0.0
21	0.003996266024112119
22	1.1257087391865122E-4
23	0.002926842721884932
24	0.008442815543898842
25	0.0
26	0.0418060083015391
27	0.036796604412159116
28	0.05798807142734521
29	0.6311426759841579
30	0.061055627741628454
31	0.009906236904841308
32	0.09205483214697704
33	0.03477032868162339
34	0.017575127690549423
35	0.010243949526597261
36	0.018391266526459643
37	0.03765495732578883
38	0.03350390635003857
39	0.025750587408891468
40	0.034277831108229295
41	0.022134248084254796
42	0.0220920340065353
43	0.02327402818268114
44	0.014282429628428874
45	0.006205469424765649
46	0.03551611072133446
47	0.015225210697497576
48	0.01484528399802213
49	0.016097634970367126
50	0.01857419419657745
51	0.01715298691335448
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7106634.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	12.433756183572516
#Duplication Level	Relative count
1	100.0
2	6.279644710936101
3	0.9659701091820297
4	0.5268927868265617
5	0.3658045462681224
6	0.35014318954716306
7	0.3473465187041346
8	0.3277698228029354
9	0.333922498657598
10++	2.3693395382137106
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG	67134	0.9446666312068414	TruSeq Adapter, Index 1 (97% over 35bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2663490	3.1673365	3.5250106	7
TTTTT	2635345	3.1360211	3.5838673	9
GAAGA	745145	2.1242838	12.809804	6
CTCCA	510765	2.0802696	16.925629	24
GAGCA	478510	2.056181	17.93211	9
GGAAG	451420	1.9925851	18.543642	5
TCCAG	450780	1.8859444	17.088581	25
CTGAA	674345	1.8717501	12.149798	19
CAGAA	653805	1.8144886	11.603837	38
CGGAA	417310	1.7932017	17.926035	4
TCTCG	388520	1.6256883	16.652134	43
TCGGA	365425	1.570465	17.72771	3
AGAGC	362375	1.5571431	17.444841	8
TCAGA	556405	1.5443892	11.352004	37
AGCAC	367430	1.5370189	17.009962	10
CACAC	377085	1.5355998	16.562958	12
TCTGA	547830	1.5207969	11.821993	18
GCACA	353895	1.4803997	16.949072	11
AAGAG	505475	1.4410248	12.09298	7
TCACA	507690	1.3718244	11.10549	30
TGAAC	487980	1.3544648	11.658073	20
CCAGT	321740	1.346075	16.53603	26
ATCGG	312230	1.3418521	17.201036	2
CTCGT	319450	1.336678	16.191017	44
CGTCT	311435	1.3031408	16.79577	16
CACGT	308445	1.2904524	16.759865	14
GTCAC	307090	1.2847832	16.436275	29
TGTCA	459295	1.2750202	11.1368885	35
GAATC	458965	1.2739291	11.126242	40
GTCAG	295540	1.2701243	16.568132	36
GTCTG	292945	1.259145	17.213402	17
ACTCC	307810	1.2536641	16.136429	23
AACTC	454720	1.2286946	11.188406	22
CACAT	447690	1.2096989	10.824349	31
ACACG	288750	1.2078876	16.629889	13
ATCTC	446750	1.2073249	10.8512945	42
GAACT	425745	1.1817218	11.471669	21
ACGTC	282130	1.1803572	16.70113	15
GATCG	272795	1.1723745	16.879593	1
AGAAT	635170	1.1696532	7.7113585	39
CAGTC	277660	1.1616559	16.298798	27
AGTCA	379165	1.0524317	11.115634	28
CATGT	378240	1.0500085	10.991539	33
ACATG	376290	1.0444517	11.005223	32
ATGTC	375080	1.0412363	10.91233	34
AATCT	568180	1.0187016	7.3309913	41
TCGTA	336425	0.93392855	10.811794	45
CGTAT	286785	0.79612607	10.650888	46
GTATG	276015	0.78708893	10.831784	47
>>END_MODULE