##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW243_AGTTCC_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6921054	
Filtered Sequences	0	
Sequence length	51	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71102869591828	34.0	31.0	34.0	30.0	34.0
2	32.03132196338881	34.0	31.0	34.0	30.0	34.0
3	32.444980489965836	34.0	31.0	34.0	30.0	34.0
4	36.090223541096485	37.0	35.0	37.0	35.0	37.0
5	36.068918838084485	37.0	35.0	37.0	35.0	37.0
6	36.08480760300382	37.0	35.0	37.0	35.0	37.0
7	36.08648697149307	37.0	35.0	37.0	35.0	37.0
8	36.080555938445215	37.0	35.0	37.0	35.0	37.0
9	37.6154264942883	39.0	38.0	39.0	35.0	39.0
10	37.72295101295265	39.0	38.0	39.0	35.0	39.0
11	37.763566503021075	39.0	38.0	39.0	35.0	39.0
12	37.74463860562278	39.0	38.0	39.0	35.0	39.0
13	37.738590971837525	39.0	38.0	39.0	35.0	39.0
14	39.18374845796608	40.0	39.0	41.0	36.0	41.0
15	39.14347106669013	40.0	39.0	41.0	36.0	41.0
16	39.13692639878261	40.0	39.0	41.0	36.0	41.0
17	39.10571988023789	40.0	38.0	41.0	36.0	41.0
18	39.087231077809825	40.0	38.0	41.0	36.0	41.0
19	39.11532708746385	40.0	39.0	41.0	36.0	41.0
20	39.073850601367944	40.0	39.0	41.0	36.0	41.0
21	39.02898532506754	40.0	39.0	41.0	36.0	41.0
22	38.97402722186534	40.0	38.0	41.0	36.0	41.0
23	38.90954412434869	40.0	38.0	41.0	35.0	41.0
24	38.81521571714366	40.0	38.0	41.0	35.0	41.0
25	38.78986899972172	40.0	38.0	41.0	35.0	41.0
26	38.68131862574689	40.0	38.0	41.0	35.0	41.0
27	38.5736610348655	40.0	38.0	41.0	34.0	41.0
28	38.48620036774746	40.0	38.0	41.0	34.0	41.0
29	38.18957603856291	40.0	38.0	41.0	34.0	41.0
30	38.16995330480011	40.0	38.0	41.0	34.0	41.0
31	38.41639712679601	40.0	38.0	41.0	34.0	41.0
32	38.38456238023862	40.0	38.0	41.0	34.0	41.0
33	38.40514681723333	40.0	38.0	41.0	34.0	41.0
34	38.52937023753896	40.0	38.0	41.0	34.0	41.0
35	38.582421550243645	40.0	38.0	41.0	34.0	41.0
36	38.566878830883276	40.0	38.0	41.0	34.0	41.0
37	38.5331253014353	40.0	38.0	41.0	34.0	41.0
38	38.478609038449925	40.0	38.0	41.0	34.0	41.0
39	38.40249230825247	40.0	38.0	41.0	34.0	41.0
40	38.35461824167244	40.0	38.0	41.0	34.0	41.0
41	38.33350917360275	40.0	38.0	41.0	34.0	41.0
42	38.27020161957991	40.0	38.0	41.0	34.0	41.0
43	38.234182683735746	40.0	38.0	41.0	33.0	41.0
44	38.15993431058333	40.0	38.0	41.0	33.0	41.0
45	38.055471319830765	40.0	38.0	41.0	33.0	41.0
46	38.01799552495906	40.0	38.0	41.0	33.0	41.0
47	37.916550138172596	40.0	37.0	41.0	33.0	41.0
48	37.83474684636184	40.0	37.0	41.0	33.0	41.0
49	37.78138402041076	40.0	37.0	41.0	33.0	41.0
50	37.69731387733718	40.0	37.0	41.0	33.0	41.0
51	36.66816802758655	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	0.0
14	7.0
15	20.0
16	67.0
17	147.0
18	321.0
19	698.0
20	1370.0
21	2164.0
22	3675.0
23	6075.0
24	9737.0
25	14691.0
26	21100.0
27	29792.0
28	41062.0
29	55398.0
30	72258.0
31	94138.0
32	121166.0
33	156216.0
34	209604.0
35	292758.0
36	436463.0
37	724328.0
38	1499284.0
39	3127535.0
40	974.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.092929610659645	27.86079928855501	32.33563446622544	21.710636634559897
2	19.148892516788994	30.321180895450446	32.5525465561382	17.977380031622356
3	18.05476449107318	30.294923287695774	32.278522895501176	19.371789325729868
4	18.331008542918465	29.905026026382686	31.543129124552415	20.220836306146435
5	19.718774047999048	30.875672982756676	31.26502119474866	18.14053177449562
6	19.769647802198914	31.189209042437753	30.244121776827633	18.797021378535696
7	18.758963591383623	32.19867956528009	30.98311326569624	18.059243577640054
8	18.03712266946624	31.885548068256657	31.536742814028035	18.540586448249066
9	19.42057363533752	30.103920617848267	31.666472091236997	18.809033655577213
10	18.433854149960396	31.48768959178761	31.10664936294385	18.971806895308145
11	19.595743654073498	30.36814912873097	30.99202809283095	19.044079124364583
12	18.444314984393994	30.43393390659862	30.77532699499238	20.346424114015004
13	18.453417644191187	31.788510825085314	30.713443357037818	19.044628173685684
14	18.4376974952081	30.77599163364424	30.765949810534636	20.020361060613023
15	18.43750966254562	31.9111655536859	30.78739163139025	18.863933152378237
16	18.490911933355818	30.739176431797816	30.78375056747137	19.986161067374997
17	19.73323716301014	30.65439165768682	30.7638403052483	18.84853087405473
18	18.664758286815854	30.468162797169335	31.889102440177464	18.97797647583735
19	18.764394604476003	30.398457100683196	30.611706754345764	20.22544154049504
20	18.755438116795506	30.41406699037459	31.700330614383297	19.130164278446607
21	19.961735698316282	30.363999248065003	30.511525311123222	19.162739742495496
22	18.73824870716815	31.52683401803224	30.578913855085798	19.15600341971381
23	18.76788170896868	31.5122942641698	30.567479705104695	19.152344321756832
24	18.804268759488227	30.361489725042624	30.514833063551944	20.319408451917212
25	18.782876134184185	30.448281432278957	31.64314279299078	19.125699640546078
26	18.829208062476617	30.430032673370945	30.53944018737898	20.201319076773462
27	18.876934740079772	30.44212202964054	30.41661095906449	20.264332271215203
28	18.851032049213604	31.620103881504303	30.364059451401104	19.164804617880986
29	20.122235631097602	30.362301350742253	30.37812059263998	19.13734242552016
30	19.010012498454863	30.310842138515266	31.401323289269474	19.2778220737604
31	18.93783716493394	30.272850268966096	30.33065048800002	20.458662078099945
32	18.931318066696488	31.495352660815172	30.348887034899114	19.224442237589223
33	18.953004229534066	30.348926804330677	30.30714167298647	20.390927293148785
34	18.943412771574316	31.523813336150337	30.362839949750597	19.16993394252475
35	20.095740863861305	30.42799732441162	30.358853392756174	19.1174084189709
36	18.981684400460765	30.408626559086127	31.448809719416882	19.160879321036226
37	18.94616051836872	30.483749810109835	31.44010246191609	19.129987209605357
38	18.973135921251007	30.476789810014548	30.311515317772912	20.238558950961533
39	19.069267256909335	30.378805604785803	30.23127625174855	20.320650886556315
40	20.145229595155158	30.372887970283436	30.217728764074174	19.26415367048723
41	19.066769911210272	30.356800656232956	31.325180714081117	19.251248718475658
42	19.00575701677969	31.3908291225622	30.292723984024413	19.310689876633703
43	18.97389213969186	30.32094720316694	31.39409034224647	19.31107031489473
44	18.97834321012738	30.281168186612522	30.348306012650404	20.392182590609693
45	19.01892979045537	30.350231323004596	31.3987925369757	19.23204634956433
46	18.990197584742926	30.397633856902573	30.304768261676024	20.307400296678477
47	20.14190700178106	30.467809003580186	30.269832840199278	19.120451154439472
48	19.081817844195477	30.391790918062217	31.34220408746015	19.18418715028216
49	19.13105391079064	31.426744830793318	30.226378181751972	19.21582307666407
50	19.172093612062326	30.315188664630384	31.275824475648978	19.23689324765831
51	20.244292335762943	30.203127036696742	30.166999378315257	19.385581249225062
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.80356624521954
2	37.12627254841135
3	37.42655381680305
4	38.5518448490649
5	37.85930582249467
6	38.56666918073461
7	36.81820716902367
8	36.57770911771531
9	38.229607290914736
10	37.40566104526854
11	38.63982277843808
12	38.790739098409
13	37.49804581787687
14	38.458058555821125
15	37.30144281492385
16	38.47707300073082
17	38.581768037064876
18	37.6427347626532
19	38.98983614497104
20	37.88560239524211
21	39.12447544081178
22	37.89425212688196
23	37.920226030725516
24	39.123677211405436
25	37.90857577473027
26	39.03052713925008
27	39.14126701129497
28	38.0158366670946
29	39.25957805661777
30	38.28783457221526
31	39.39649924303389
32	38.15576030428571
33	39.34393152268285
34	38.113346714099066
35	39.213149282832205
36	38.142563721496984
37	38.07614772797408
38	39.21169487221254
39	39.38991814346565
40	39.40938326564239
41	38.31801862968593
42	38.31644689341339
43	38.28496245458659
44	39.37052580073707
45	38.2509761400197
46	39.2975978814214
47	39.262358156220536
48	38.26600499447764
49	38.34687698745471
50	38.40898685972064
51	39.629873584988005
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	59.0
2	63.0
3	123.0
4	183.0
5	421.0
6	659.0
7	1357.5
8	2056.0
9	3648.5
10	5241.0
11	8845.5
12	12450.0
13	18868.0
14	25286.0
15	35632.0
16	45978.0
17	61771.5
18	77565.0
19	99088.0
20	120611.0
21	146701.0
22	172791.0
23	202183.5
24	231576.0
25	259258.5
26	313128.5
27	339316.0
28	361886.5
29	384457.0
30	402442.0
31	420427.0
32	431242.5
33	442058.0
34	452255.5
35	462453.0
36	468071.5
37	473690.0
38	472522.0
39	471354.0
40	462803.5
41	454253.0
42	440900.0
43	427547.0
44	408113.5
45	388680.0
46	362176.5
47	335673.0
48	312097.5
49	288522.0
50	296579.0
51	304636.0
52	246533.5
53	188431.0
54	166790.0
55	145149.0
56	128279.0
57	111409.0
58	98604.5
59	85800.0
60	75086.0
61	64372.0
62	55386.0
63	46400.0
64	39952.5
65	33505.0
66	28629.5
67	23754.0
68	20241.5
69	16729.0
70	14060.0
71	11391.0
72	9595.5
73	7800.0
74	6416.0
75	4015.5
76	2999.0
77	2387.5
78	1776.0
79	1404.5
80	1033.0
81	772.0
82	511.0
83	369.0
84	227.0
85	172.0
86	117.0
87	82.5
88	48.0
89	36.5
90	25.0
91	18.5
92	12.0
93	11.0
94	10.0
95	6.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047709496270365755
2	1.444866634475038E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.889733268950076E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	8.38022647995522E-4
20	0.0
21	0.0035110259217743423
22	1.0114066441325267E-4
23	0.0032509499275688355
24	0.009189351795261242
25	0.0
26	0.0416410564055706
27	0.035962730532083695
28	0.056956642731006
29	0.626407480710308
30	0.060669949981606845
31	0.009608363119259002
32	0.09011633199220812
33	0.033593149251544635
34	0.016919388289702696
35	0.010215207105738519
36	0.017555129608871713
37	0.03729200783580073
38	0.03266843460548061
39	0.02532851210234742
40	0.03401216057554239
41	0.022539919497810593
42	0.021644102184436072
43	0.022641060162223847
44	0.013885168357305116
45	0.006184029195553163
46	0.035442578543672686
47	0.01486767766874814
48	0.014607601674542633
49	0.015676802984054165
50	0.01823421692707498
51	0.01658706896377344
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6921054.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.923571613710937
#Duplication Level	Relative count
1	100.0
2	31.007456789413943
3	6.728351604143619
4	1.7025617448025172
5	0.7665119754026523
6	0.5567448743552535
7	0.468384074941452
8	0.40444821195097774
9	0.3045933248085516
10++	1.7370440079883909
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG	67700	0.9781747115396008	TruSeq Adapter, Index 8 (97% over 37bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3235220	3.672909	3.9640453	13
TTTTT	3254130	3.5534687	4.0342226	1
GGAAG	423985	2.0173655	19.87458	5
CTCCA	445845	1.9775242	18.236933	24
TCCAG	427720	1.9370327	18.548485	25
GAGCA	413435	1.9266456	19.190561	9
CTGAA	668350	1.9168063	12.575057	19
GAAGA	642455	1.8959818	12.852141	6
CGGAA	394510	1.8384533	19.397318	4
TTCCG	397695	1.7871037	17.546288	36
CACAG	344385	1.5718074	17.839298	31
TCTCG	339205	1.5242699	17.476612	43
CACAC	339210	1.516297	18.08587	12
TCTGA	525485	1.4953983	12.04026	18
AGAGC	320865	1.4952607	18.787931	8
GCACA	325350	1.4849299	18.438398	11
AGCAC	323435	1.4761895	18.53698	10
TCGGA	318495	1.4727176	18.839931	3
CAGTT	497885	1.4168555	11.482588	33
CCAGT	312275	1.4142121	17.932198	26
GTTCC	313745	1.4098616	17.231281	35
AAGAG	453835	1.3393357	12.315163	7
ATCGG	285145	1.3185076	18.39157	2
TGAAC	457065	1.3108475	11.947646	20
TCACA	466490	1.3103192	11.329895	30
GTCTG	279510	1.2824382	18.320013	17
ACTCC	287010	1.2730191	17.645649	23
GTCAC	275670	1.2484376	17.584557	29
TCCGT	271170	1.2185441	16.9781	37
CTCGT	270830	1.2170162	17.106274	44
CACGT	265160	1.2008406	17.995707	14
CGTCT	267175	1.200592	17.859661	16
AACTC	425050	1.193919	11.546726	22
ACAGT	416205	1.1936625	11.385424	32
GAACT	403955	1.1585298	11.732816	21
CAGTC	253940	1.1500282	17.641176	27
GATCG	248660	1.1498013	18.069016	1
ACACG	251215	1.1465703	18.159792	13
ATCTC	410550	1.1442556	10.986891	42
AGTTC	398955	1.1353254	11.176195	34
ACGTC	243660	1.1034728	17.942753	15
AGTCA	350475	1.005151	11.396804	28
CCGTA	214210	0.9701015	16.73512	38
CGTAT	328055	0.9335621	10.719224	46
TCGTA	279950	0.7966673	10.832664	45
GTATC	272125	0.7743992	10.769484	40
GTATG	260990	0.7583328	10.975018	47
TATCT	382850	0.67050934	6.826267	41
>>END_MODULE