FastQCFastQC Report
Wed 23 Jul 2014
LW242_AGTCAA_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW242_AGTCAA_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6313438
Filtered Sequences 0
Sequence length 51
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC 395980 6.272018510358382 TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CAGTC 1059850 4.8002768 94.67202 27
TTTTT 2504545 3.74005 4.2361135 1
GGAAG 766025 3.6934004 104.479195 5
AGTCA 1138675 3.5279636 64.78312 28
GAGCA 745335 3.4528892 99.69417 9
TCCAG 760345 3.4437575 95.65545 25
AAAAA 2482615 3.3989382 3.6470342 12
CGGAA 726970 3.3678102 100.77523 4
CTCCA 767790 3.3412738 92.05169 24
TCTCG 677015 3.1200614 95.65407 41
TCGGA 659760 3.109998 102.18888 3
AGAGC 669780 3.1028686 99.41357 8
CACAG 690425 3.0732327 93.02651 31
GTCTG 639290 3.0663025 101.96817 17
CCAGT 665315 3.0133471 94.99306 26
ATCGG 637140 3.003371 100.70034 2
GAAGA 946310 2.9989347 69.02769 6
AGCAC 672315 2.992621 95.802925 10
GCACA 672010 2.9912634 95.62004 11
CTGAA 947335 2.9351335 66.71536 19
CACAC 681690 2.9155037 91.77244 12
GATCG 612770 2.8884952 99.77164 1
ATGCC 635970 2.8804376 93.10214 47
CGTCT 623955 2.8755312 98.05038 16
GTCAC 632435 2.864427 94.76534 29
CTCGT 615790 2.8379025 94.755295 42
CACGT 615520 2.7878156 96.57753 14
ACTCC 634805 2.7625487 91.959984 23
ACGTC 599905 2.717092 96.420616 15
ACACG 600605 2.6734242 94.98151 13
TCTGA 831570 2.6215975 67.67382 18
TCAAA 1276330 2.599186 43.03587 36
AAGAG 780655 2.4739604 68.42353 7
TGAAC 777950 2.410327 66.1008 20
TCACA 780775 2.3243306 62.34291 30
CAAAT 1117810 2.2763674 42.920128 37
GAACT 732890 2.2707176 65.90728 21
ACAGT 732495 2.2694933 64.40158 32
GTCAA 731950 2.2678049 64.12832 35
ATCTC 740795 2.2439492 63.13973 40
AACTC 748535 2.2283537 63.42518 22
AAATC 1093285 2.2264235 42.94078 38
GTATG 614750 2.0170562 67.321915 45
TATGC 628715 1.9820794 64.57752 46
TCGTA 621090 1.958041 64.78784 43
CGTAT 598470 1.8867294 64.64281 44
AATCT 849040 1.7593225 43.22505 39
TGCCG 234845 1.6182777 5.1103406 47
Produced by FastQC (version 0.10.1)