##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW242_AGTCAA_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6313438	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.668576772275266	34.0	31.0	34.0	30.0	34.0
2	31.969813119254518	34.0	31.0	34.0	30.0	34.0
3	32.39906624568104	34.0	31.0	34.0	30.0	34.0
4	36.06411055276064	37.0	35.0	37.0	35.0	37.0
5	36.04124535633358	37.0	35.0	37.0	35.0	37.0
6	36.05231666169843	37.0	35.0	37.0	35.0	37.0
7	36.01880940305425	37.0	35.0	37.0	35.0	37.0
8	36.042165932412736	37.0	35.0	37.0	35.0	37.0
9	37.55781382505063	39.0	38.0	39.0	35.0	39.0
10	37.63193192045285	39.0	37.0	39.0	35.0	39.0
11	37.69297979959572	39.0	37.0	39.0	35.0	39.0
12	37.688772741571235	39.0	37.0	39.0	35.0	39.0
13	37.69166450989144	39.0	37.0	39.0	35.0	39.0
14	39.16456770463256	40.0	39.0	41.0	36.0	41.0
15	39.08150931394274	40.0	38.0	41.0	36.0	41.0
16	39.117477989013274	40.0	39.0	41.0	36.0	41.0
17	39.070304800649026	40.0	38.0	41.0	36.0	41.0
18	39.017331286059985	40.0	38.0	41.0	36.0	41.0
19	39.07265534246159	40.0	39.0	41.0	36.0	41.0
20	39.05017519772903	40.0	39.0	41.0	36.0	41.0
21	38.98108304857037	40.0	38.0	41.0	35.0	41.0
22	38.90282568705038	40.0	38.0	41.0	35.0	41.0
23	38.80843147584565	40.0	38.0	41.0	35.0	41.0
24	38.785982217612656	40.0	38.0	41.0	35.0	41.0
25	38.74155396790148	40.0	38.0	41.0	35.0	41.0
26	38.68120032223331	40.0	38.0	41.0	35.0	41.0
27	38.590413179000095	40.0	38.0	41.0	34.0	41.0
28	38.30540063908128	40.0	38.0	41.0	34.0	41.0
29	38.10540516910121	40.0	38.0	41.0	34.0	41.0
30	37.96991132248388	40.0	38.0	41.0	33.0	41.0
31	38.29364412860315	40.0	38.0	41.0	34.0	41.0
32	38.20934140796187	40.0	38.0	41.0	34.0	41.0
33	38.3372488967184	40.0	38.0	41.0	34.0	41.0
34	38.39714732290077	40.0	38.0	41.0	34.0	41.0
35	38.48482855141683	40.0	38.0	41.0	34.0	41.0
36	38.270279362844775	40.0	38.0	41.0	34.0	41.0
37	38.358235402010756	40.0	38.0	41.0	34.0	41.0
38	38.366067584729585	40.0	38.0	41.0	34.0	41.0
39	38.237623146057665	40.0	38.0	41.0	33.0	41.0
40	38.22258538058028	40.0	38.0	41.0	33.0	41.0
41	38.27114972856311	40.0	38.0	41.0	34.0	41.0
42	38.23287248564095	40.0	38.0	41.0	33.0	41.0
43	38.20584236354265	40.0	38.0	41.0	33.0	41.0
44	38.158555449503105	40.0	38.0	41.0	33.0	41.0
45	37.94136491084572	40.0	37.0	41.0	33.0	41.0
46	37.726537426993026	40.0	37.0	41.0	33.0	41.0
47	37.67590368353978	40.0	37.0	41.0	33.0	41.0
48	37.702767968894285	40.0	37.0	41.0	33.0	41.0
49	37.61724341000894	40.0	37.0	41.0	32.0	41.0
50	37.57675596085683	40.0	37.0	41.0	32.0	41.0
51	36.61935335390955	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	7.0
11	11.0
12	11.0
13	2.0
14	12.0
15	27.0
16	52.0
17	131.0
18	266.0
19	647.0
20	1354.0
21	1961.0
22	3525.0
23	5897.0
24	9308.0
25	14344.0
26	20760.0
27	29167.0
28	40312.0
29	54516.0
30	71509.0
31	92597.0
32	117439.0
33	152514.0
34	202784.0
35	280604.0
36	414706.0
37	675319.0
38	1358227.0
39	2764572.0
40	856.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.09960437153136	24.844456811905808	29.65357867390459	20.402360142658242
2	18.500309261123302	34.2632180421593	29.498505963869114	17.737966732848285
3	17.34641252515666	27.619388992178273	37.21034403125524	17.823854451409833
4	17.599507590000883	27.27339684020022	29.364903876461607	25.76219169333729
5	25.237485503144246	27.953628435093524	28.878623659565516	17.93026240219671
6	25.599665348737087	28.404349579420913	27.616046914533733	18.379938157308267
7	18.450327697840702	35.66023773417906	28.24910928087042	17.64032528710981
8	17.804562268608638	35.647312922056095	28.55441044958389	17.993714359751376
9	25.039835677434603	28.021920234876855	28.714506648994302	18.223737438694236
10	18.074256847061775	35.26566666212609	28.279884905815184	18.380191584996954
11	25.213916728096482	28.094169927700246	28.27584590202676	18.416067442176512
12	17.9732817523511	28.187209567908962	28.20550387918595	25.634004800553996
13	17.949443710384106	35.4347029304794	28.208323262222578	18.407530096913916
14	17.902385356441293	28.40095998408474	28.288057948775297	25.40859671069867
15	17.83438753972083	35.48865134970835	28.367095709184127	18.309865401386695
16	17.992811523610435	28.289103337991122	28.297561487101007	25.42052365129744
17	25.180638504725948	28.214326330598322	28.343004239528447	18.262030925147283
18	18.149604066754122	28.11396896587881	35.33239734040312	18.404029626963947
19	18.117529176857964	28.070068175691237	28.246439236961923	25.565963410488873
20	18.30779046218558	28.033980851637413	35.19359816315612	18.464630523020897
21	25.328648756359495	28.05431921281113	28.098971694065085	18.518060336764293
22	18.20131053959575	35.19009476623642	28.125947385177312	18.482647308990515
23	18.14975282909275	35.116864660523916	28.171100019435336	18.562282490947997
24	18.140282562313544	28.07105417770958	28.175364845768318	25.61329841420855
25	18.18376928703505	28.006468108184478	35.1847598725132	18.62500273226727
26	18.22807636085615	28.050674832529822	28.09439336378366	25.626855442830376
27	18.2601152222687	28.168247962378473	27.96979376608232	25.601843049270514
28	18.44910624715725	35.07928005312279	27.88955785423247	18.582055845487496
29	25.425972861034975	28.059971062566124	28.015439673355726	18.49861640304318
30	18.45705616857069	27.946178641038898	34.867473667961306	18.729291522429108
31	18.347226519042955	28.066500369803798	27.88873475777099	25.69753835338226
32	18.332863289995814	34.97807283499862	27.897032449160413	18.792031425845153
33	18.320297546766582	28.16907143708218	27.877105098375242	25.63352591777599
34	18.49528911498349	34.981897540745976	27.903482699612603	18.61933064465793
35	25.359894285063454	28.082525955887267	28.06042808719898	18.497151671850293
36	18.396817943614437	28.076217018070476	34.89746207303851	18.629502965276576
37	18.32153367932131	28.26266692145173	27.9614723154163	25.454327083810657
38	18.354836414810546	35.185499430318465	27.932220018281146	18.527444136589843
39	18.414926391815882	35.18400224083458	27.8431935441657	18.55787782318383
40	18.446619170563718	34.98457947886323	27.96177390259998	18.60702744797307
41	18.41525760717856	27.999407490023177	34.79502164879367	18.79031325400459
42	18.393489256912698	27.961066210065916	28.06022415176314	25.585220381258246
43	18.38127552545666	27.893811882325338	34.86750249244267	18.857410099775336
44	18.533998370884003	27.967948679361015	27.938816587581776	25.559236362173205
45	25.307059923017217	28.014541192124316	28.066148168094912	18.612250716763555
46	18.517541441278716	28.09664825536257	34.84149166421077	18.544318639147946
47	18.46369484587951	34.93095749886615	28.138262743124383	18.46708491212996
48	18.5941159110304	28.06462901161062	34.805736415663766	18.53551866169522
49	25.434838846221417	27.909194930511806	27.892434563678385	18.763531659588395
50	18.654385174762382	27.94171952187221	27.76023462499273	25.643660678372676
51	18.692425432469655	27.860538179532373	27.74329398368245	25.70374240431552
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	45.5019645141896
2	36.238275993971584
3	35.170266976566495
4	43.361699283338176
5	43.16774790534096
6	43.979603506045365
7	36.09065298495051
8	35.798276628360014
9	43.26357311612884
10	36.454448432058726
11	43.629984170273
12	43.60728655290509
13	36.35697380729802
14	43.31098206713996
15	36.144252941107524
16	43.41333517490787
17	43.442669429873234
18	36.55363369371807
19	43.68349258734684
20	36.77242098520648
21	43.846709093123785
22	36.68395784858627
23	36.71203532004075
24	43.7535809765221
25	36.808772019302324
26	43.854931803686526
27	43.861958271539216
28	37.03116209264474
29	43.92458926407815
30	37.186347690999796
31	44.04476487242521
32	37.124894715840966
33	43.95382346454257
34	37.114619759641414
35	43.85704595691375
36	37.026320908891016
37	43.775860763131966
38	36.88228055140039
39	36.97280421499971
40	37.05364661853679
41	37.205570861183155
42	43.97870963817094
43	37.238685625231994
44	44.09323473305721
45	43.919310639780775
46	37.06186008042666
47	36.93077975800947
48	37.129634572725614
49	44.19837050580981
50	44.29804585313506
51	44.39616783678518
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22.0
1	20.5
2	19.0
3	57.0
4	95.0
5	229.5
6	364.0
7	759.5
8	1155.0
9	2236.5
10	3318.0
11	5693.5
12	8069.0
13	12598.0
14	17127.0
15	24586.0
16	32045.0
17	43486.0
18	54927.0
19	71252.0
20	87577.0
21	107402.5
22	127228.0
23	150981.5
24	174735.0
25	197038.0
26	241450.0
27	263559.0
28	283577.0
29	303595.0
30	320439.0
31	337283.0
32	348420.0
33	359557.0
34	371060.5
35	382564.0
36	389621.5
37	396679.0
38	397760.0
39	398841.0
40	394426.5
41	390012.0
42	380804.0
43	371596.0
44	357910.0
45	344224.0
46	322558.0
47	300892.0
48	289943.0
49	278994.0
50	458578.5
51	638163.0
52	418314.5
53	198466.0
54	173283.0
55	148100.0
56	132874.5
57	117649.0
58	105251.0
59	92853.0
60	82751.0
61	72649.0
62	63829.5
63	55010.0
64	47875.5
65	40741.0
66	35686.5
67	30632.0
68	26295.5
69	21959.0
70	18818.0
71	15677.0
72	13240.5
73	10804.0
74	8965.0
75	5752.5
76	4379.0
77	3505.0
78	2631.0
79	2038.0
80	1445.0
81	1066.0
82	687.0
83	522.0
84	357.0
85	258.0
86	159.0
87	115.0
88	71.0
89	55.0
90	39.0
91	25.5
92	12.0
93	8.5
94	5.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04707419317335499
2	4.751769162855484E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	3.1678461085703226E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	6.969261438854709E-4
20	0.0
21	0.0037855760997415355
22	1.267138443428129E-4
23	0.0030886499558560646
24	0.008949165256711161
25	0.0
26	0.041387909408471264
27	0.03418105951147378
28	0.05588080535518049
29	0.6210879080462974
30	0.06056921759586457
31	0.009994554472539368
32	0.08725832106056954
33	0.033167348756731274
34	0.016409442842394272
35	0.009693609092225188
36	0.016504478225651383
37	0.035416519493816204
38	0.0319477280049317
39	0.023822202736448826
40	0.03235954799904584
41	0.02071771355004991
42	0.020369250478107174
43	0.02149383584664964
44	0.01286145520079551
45	0.005353659923483845
46	0.03323070567890268
47	0.013891005186080866
48	0.013542542114138129
49	0.014682966713223444
50	0.01690045898922267
51	0.015395732087651768
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6313438.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	32.02820041190582
#Duplication Level	Relative count
1	100.0
2	24.847151552778264
3	4.995073758654664
4	1.3833965421834722
5	0.7672789602256623
6	0.5317641342636956
7	0.4345889625200761
8	0.33943827352111533
9	0.2456372396852604
10++	1.4211868867504354
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC	395980	6.272018510358382	TruSeq Adapter, Index 8 (97% over 36bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAGTC	1059850	4.8002768	94.67202	27
TTTTT	2504545	3.74005	4.2361135	1
GGAAG	766025	3.6934004	104.479195	5
AGTCA	1138675	3.5279636	64.78312	28
GAGCA	745335	3.4528892	99.69417	9
TCCAG	760345	3.4437575	95.65545	25
AAAAA	2482615	3.3989382	3.6470342	12
CGGAA	726970	3.3678102	100.77523	4
CTCCA	767790	3.3412738	92.05169	24
TCTCG	677015	3.1200614	95.65407	41
TCGGA	659760	3.109998	102.18888	3
AGAGC	669780	3.1028686	99.41357	8
CACAG	690425	3.0732327	93.02651	31
GTCTG	639290	3.0663025	101.96817	17
CCAGT	665315	3.0133471	94.99306	26
ATCGG	637140	3.003371	100.70034	2
GAAGA	946310	2.9989347	69.02769	6
AGCAC	672315	2.992621	95.802925	10
GCACA	672010	2.9912634	95.62004	11
CTGAA	947335	2.9351335	66.71536	19
CACAC	681690	2.9155037	91.77244	12
GATCG	612770	2.8884952	99.77164	1
ATGCC	635970	2.8804376	93.10214	47
CGTCT	623955	2.8755312	98.05038	16
GTCAC	632435	2.864427	94.76534	29
CTCGT	615790	2.8379025	94.755295	42
CACGT	615520	2.7878156	96.57753	14
ACTCC	634805	2.7625487	91.959984	23
ACGTC	599905	2.717092	96.420616	15
ACACG	600605	2.6734242	94.98151	13
TCTGA	831570	2.6215975	67.67382	18
TCAAA	1276330	2.599186	43.03587	36
AAGAG	780655	2.4739604	68.42353	7
TGAAC	777950	2.410327	66.1008	20
TCACA	780775	2.3243306	62.34291	30
CAAAT	1117810	2.2763674	42.920128	37
GAACT	732890	2.2707176	65.90728	21
ACAGT	732495	2.2694933	64.40158	32
GTCAA	731950	2.2678049	64.12832	35
ATCTC	740795	2.2439492	63.13973	40
AACTC	748535	2.2283537	63.42518	22
AAATC	1093285	2.2264235	42.94078	38
GTATG	614750	2.0170562	67.321915	45
TATGC	628715	1.9820794	64.57752	46
TCGTA	621090	1.958041	64.78784	43
CGTAT	598470	1.8867294	64.64281	44
AATCT	849040	1.7593225	43.22505	39
TGCCG	234845	1.6182777	5.1103406	47
>>END_MODULE