##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW241_CTTGTA_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9405925	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.770408545677327	34.0	31.0	34.0	30.0	34.0
2	32.086936478868374	34.0	31.0	34.0	30.0	34.0
3	32.47885083072638	34.0	31.0	34.0	30.0	34.0
4	36.10508089316043	37.0	35.0	37.0	35.0	37.0
5	36.07405821330704	37.0	35.0	37.0	35.0	37.0
6	36.09085390325779	37.0	35.0	37.0	35.0	37.0
7	36.08600344995309	37.0	35.0	37.0	35.0	37.0
8	36.07554376629624	37.0	35.0	37.0	35.0	37.0
9	37.60527263400463	39.0	38.0	39.0	35.0	39.0
10	37.71998532839673	39.0	38.0	39.0	35.0	39.0
11	37.76401842455686	39.0	38.0	39.0	35.0	39.0
12	37.74296084648772	39.0	38.0	39.0	35.0	39.0
13	37.72743403758801	39.0	38.0	39.0	35.0	39.0
14	39.176484396803076	40.0	39.0	41.0	36.0	41.0
15	39.131965755627434	40.0	39.0	41.0	36.0	41.0
16	39.12101967642736	40.0	39.0	41.0	36.0	41.0
17	39.09651671685666	40.0	38.0	41.0	36.0	41.0
18	39.07465709114202	40.0	38.0	41.0	36.0	41.0
19	39.10252877840298	40.0	39.0	41.0	36.0	41.0
20	39.06425269178736	40.0	39.0	41.0	36.0	41.0
21	39.01781440953442	40.0	39.0	41.0	36.0	41.0
22	38.96203286758081	40.0	38.0	41.0	36.0	41.0
23	38.89151061697813	40.0	38.0	41.0	35.0	41.0
24	38.79723652910267	40.0	38.0	41.0	35.0	41.0
25	38.774643961120255	40.0	38.0	41.0	35.0	41.0
26	38.661070442300996	40.0	38.0	41.0	35.0	41.0
27	38.55538854498627	40.0	38.0	41.0	34.0	41.0
28	38.46220015575289	40.0	38.0	41.0	34.0	41.0
29	38.160748145450874	40.0	38.0	41.0	34.0	41.0
30	38.15343094910921	40.0	38.0	41.0	34.0	41.0
31	38.40148597825307	40.0	38.0	41.0	34.0	41.0
32	38.37018134845855	40.0	38.0	41.0	34.0	41.0
33	38.38964960915593	40.0	38.0	41.0	34.0	41.0
34	38.53881760698709	40.0	38.0	41.0	34.0	41.0
35	38.599091317440866	40.0	38.0	41.0	35.0	41.0
36	38.60960469066041	40.0	38.0	41.0	35.0	41.0
37	38.56431100609456	40.0	38.0	41.0	34.0	41.0
38	38.50897471540545	40.0	38.0	41.0	34.0	41.0
39	38.431413284711496	40.0	38.0	41.0	34.0	41.0
40	38.38420825171368	40.0	38.0	41.0	34.0	41.0
41	38.37772584833496	40.0	38.0	41.0	34.0	41.0
42	38.324368841979926	40.0	38.0	41.0	34.0	41.0
43	38.27627064855397	40.0	38.0	41.0	34.0	41.0
44	38.20476072262962	40.0	38.0	41.0	33.0	41.0
45	38.0875572577923	40.0	38.0	41.0	33.0	41.0
46	38.03834232146227	40.0	38.0	41.0	33.0	41.0
47	37.95870507153735	40.0	37.0	41.0	33.0	41.0
48	37.90641962380096	40.0	37.0	41.0	33.0	41.0
49	37.84937685554584	40.0	37.0	41.0	33.0	41.0
50	37.75627479487663	40.0	37.0	41.0	33.0	41.0
51	36.71069575825876	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	5.0
11	5.0
12	5.0
13	9.0
14	14.0
15	35.0
16	90.0
17	191.0
18	427.0
19	934.0
20	1773.0
21	2857.0
22	4786.0
23	8073.0
24	12734.0
25	19658.0
26	28325.0
27	40098.0
28	55152.0
29	74083.0
30	97625.0
31	126549.0
32	162210.0
33	212089.0
34	282940.0
35	395239.0
36	588633.0
37	978176.0
38	2057441.0
39	4254549.0
40	1219.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.776588079345093	27.195870086377766	33.70075209051721	22.326789743759935
2	18.773899841801757	30.260612465340976	31.095682293704392	19.869805399152874
3	18.695471205649632	30.04248917570574	31.409042704465534	19.852996914179094
4	18.97286019184716	28.4146003715743	31.307043166939984	21.305496269638553
5	20.2983332314472	29.424091729415235	31.424851888570238	18.85272315056733
6	20.256306530192404	30.221801683513316	29.28689097563504	20.23500081065924
7	20.09290952245526	30.793887895129927	30.273577558825952	18.839625023588855
8	19.182823592575957	31.29293503828704	30.177265925467196	19.346975443669816
9	19.806060417913443	29.87596427313482	30.55209172011794	19.765883588833795
10	19.15121585596313	30.805412545815535	30.108830338323983	19.93454125989735
11	19.966818787094304	29.895443563498542	30.10380159314475	20.03393605626241
12	19.171001257186298	29.9749466426747	29.988066032846316	20.865986067292692
13	19.076922259107956	30.979281676177518	30.08478166687487	19.85901439783966
14	19.09358197093853	30.29936981211311	30.05208950741155	20.55495870953681
15	19.05710496309507	31.043028729231843	30.15171819890122	19.748148108771865
16	19.11338863535484	30.15182451486696	30.188386575483005	20.54640027429519
17	20.0397834343778	30.116793404157487	30.112189922841186	19.731233238623528
18	19.24607096059133	29.91607949244758	30.943485090514756	19.894364456446336
19	19.22505961443819	29.88014172868209	30.130868429986034	20.763930226893685
20	19.307617273154953	29.90224778530554	30.88556415238267	19.904570789156832
21	20.143920382894603	29.833619633884727	30.027567569464075	19.994892413756588
22	19.36067443539765	30.653082869582448	30.03541814977644	19.95082454524346
23	19.390979398925705	30.68689952162674	30.038011292390323	19.884109787057234
24	19.442729652675457	29.80944156432674	29.961752641706717	20.78607614129109
25	19.39349931027517	29.87214973540614	30.784989248797967	19.949361705520722
26	19.419279405550473	29.896364013651077	29.94416213582516	20.74019444497329
27	19.493472960288123	29.84924985466752	29.852440468986273	20.804836716058087
28	19.47126270248259	30.67799868157674	29.83234196286778	20.018396653072887
29	20.369984802854376	29.888732585444895	29.80558909160785	19.935693520092887
30	19.596312411246082	29.78823310645024	30.533954860323973	20.081499621979706
31	19.544245471487084	29.719599715853846	29.806340635781808	20.92981417687726
32	19.583127070520387	30.618109527362687	29.784921269679387	20.01384213243754
33	19.578778256789327	29.811300929010148	29.774311537882326	20.835609276318195
34	19.588306338160713	29.866579812540557	29.747197922986928	20.797915926311802
35	19.61797379828531	29.87295954675816	30.579352994383708	19.929713660572823
36	19.61349345740152	29.805840759287438	30.538166035057827	20.042499748253217
37	20.371083342993916	29.86840691860756	29.78669434733639	19.973815391062136
38	19.638512317062666	29.888767294032313	30.547336381358335	19.925384007546683
39	19.657615758752485	30.64303015434429	29.698496967167955	20.000857119735265
40	19.67112029038066	30.622652967244967	29.713490512962586	19.992736229411783
41	19.666352931218135	29.832093934754017	30.49156577666963	20.009987357358217
42	19.706414705339085	29.77592114401326	29.667083055212228	20.850581095435423
43	19.623993715717905	29.750146191228016	30.554397333242306	20.071462759811773
44	19.68165476996822	29.777895214773242	29.761339433913513	20.77911058134503
45	20.440801840652565	29.80814131516497	29.76490001445984	19.986156829722628
46	19.604274273467315	29.846647879103326	30.545991491356606	20.003086356072753
47	19.73560361851689	30.61505770206569	29.748293266766733	19.90104541265069
48	19.766934065631432	29.760906131702857	30.45078504030337	20.021374762362342
49	20.517484441886783	29.75942796247466	29.703964668520687	20.01912292711787
50	19.80089568338286	29.77517743105858	29.666513402074134	20.75741348348443
51	19.782570461661614	29.72914403082664	29.6341346447287	20.85415086278305
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	39.10337782310502
2	38.64370524095463
3	38.548468119828726
4	40.278356461485714
5	39.151056382014524
6	40.49130734085165
7	38.93253454604412
8	38.52979903624577
9	39.57194400674724
10	39.08575711586048
11	40.00075484335671
12	40.036987324478986
13	38.93593665694762
14	39.648540680475335
15	38.805253071866936
16	39.65978890965003
17	39.77101667300133
18	39.140435417037665
19	39.98898984133188
20	39.21218806231179
21	40.138812796651195
22	39.31149898064111
23	39.27508918598294
24	40.22880579396655
25	39.34286101579589
26	40.15947385052377
27	40.298309676346214
28	39.48965935555548
29	40.305678322947266
30	39.677812033225784
31	40.474059648364346
32	39.59696920295793
33	40.41438753310753
34	40.386222264472515
35	39.547687458858135
36	39.65599320565474
37	40.34489873405605
38	39.563896324609345
39	39.65847287848775
40	39.663856519792446
41	39.676340288576355
42	40.55699580077451
43	39.69545647552968
44	40.46076535131324
45	40.42695867037519
46	39.60736062954007
47	39.63664903116758
48	39.788308827993774
49	40.53660736900465
50	40.55830916686728
51	40.636721324444665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	25.5
2	25.0
3	42.0
4	59.0
5	156.5
6	254.0
7	533.5
8	813.0
9	1692.0
10	2571.0
11	4723.5
12	6876.0
13	11381.5
14	15887.0
15	24133.5
16	32380.0
17	46659.0
18	60938.0
19	81963.5
20	102989.0
21	131292.5
22	159596.0
23	193887.0
24	228178.0
25	264417.0
26	337435.0
27	374214.0
28	411496.5
29	448779.0
30	483898.5
31	519018.0
32	546853.0
33	574688.0
34	600906.0
35	627124.0
36	649605.5
37	672087.0
38	686890.0
39	701693.0
40	704389.5
41	707086.0
42	701314.0
43	695542.0
44	674621.0
45	653700.0
46	612066.5
47	570433.0
48	531556.5
49	492680.0
50	480394.5
51	468109.0
52	386241.0
53	304373.0
54	262282.5
55	220192.0
56	188449.0
57	156706.0
58	134116.5
59	111527.0
60	93929.5
61	76332.0
62	62467.0
63	48602.0
64	38931.0
65	29260.0
66	23698.0
67	18136.0
68	14272.5
69	10409.0
70	8219.0
71	6029.0
72	4791.5
73	3554.0
74	2774.0
75	1534.0
76	1074.0
77	839.0
78	604.0
79	485.0
80	366.0
81	261.0
82	156.0
83	127.5
84	99.0
85	75.0
86	51.0
87	38.5
88	26.0
89	24.0
90	22.0
91	14.0
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049575134821934055
2	5.3157982867182125E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.378957944061856E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	7.016853738468041E-4
20	0.0
21	0.003274531744618419
22	1.3821075545467353E-4
23	0.0031788473754574913
24	0.008834856752525669
25	0.0
26	0.0412718578980802
27	0.035583953731291716
28	0.05636872503235992
29	0.631867679149047
30	0.05991967828788768
31	0.0096322264955334
32	0.09067688717483927
33	0.03432942533562621
34	0.017552765942743537
35	0.01029138548308646
36	0.01737202880099512
37	0.03673216616122285
38	0.03311742332625446
39	0.024856672788694363
40	0.032904791394785735
41	0.02164593062351656
42	0.021773509782397796
43	0.022815406246594568
44	0.014405813357006357
45	0.006006852063991579
46	0.03456332046024182
47	0.01497991957197192
48	0.014629076885048518
49	0.01602181603616869
50	0.01816939854400285
51	0.01691487014833735
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	9405925.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	14.28648405047418
#Duplication Level	Relative count
1	100.0
2	7.548905868704244
3	1.1127049531658084
4	0.5057749787117312
5	0.39319003994765084
6	0.3148949302487727
7	0.2417432949096749
8	0.22688436898142061
9	0.23659982054989456
10++	3.718731962121169
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC	67435	0.7169417149296853	TruSeq Adapter, Index 12 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	3475550	3.2358778	3.512485	13
TTTTT	3483730	3.1104057	3.4565105	1
GAAGA	937400	2.0577683	9.879349	6
CTCCA	653940	1.9793838	12.593816	24
GAGCA	602275	1.9640565	13.628137	9
GGAAG	568025	1.9146547	14.070584	5
CTGAA	863890	1.8193945	9.34436	19
TCCAG	575845	1.8016133	12.888811	25
CGGAA	527470	1.7201127	13.6181755	4
TCTCG	486470	1.5092906	12.358283	41
TCACC	496135	1.5017302	12.046932	30
TCGGA	463200	1.4979194	13.33371	3
TCTGA	696595	1.4548209	8.901785	18
AGAGC	442505	1.4430366	13.13737	8
CACAC	466655	1.4243845	12.327999	12
AGCAC	448300	1.4143728	12.75885	10
AAGAG	630300	1.3836262	9.224813	7
GCACA	437990	1.3818454	12.697372	11
TGAAC	620115	1.3059924	8.829419	20
CCAGT	404630	1.2659428	12.2501	26
ATCGG	384290	1.2427365	12.815912	2
CTCGT	394315	1.2233764	11.913616	42
CACGT	381095	1.1923102	12.398234	14
CGTCT	382000	1.1851686	12.267162	16
GTCAC	378000	1.182627	12.067558	29
CACCT	389680	1.1795062	11.67106	31
AACTC	574075	1.1696962	8.421771	22
CCTTG	374500	1.1618997	11.896942	33
ACTCC	381600	1.1550491	11.913745	23
GTCTG	358005	1.1480738	12.552884	17
CTTGT	551310	1.1417891	8.263968	34
ATCTC	564300	1.1401851	8.159203	40
GAACT	535020	1.1267782	8.651882	21
ACACG	350835	1.1068739	12.435158	13
ATGCC	351760	1.1005315	11.937047	47
GATCG	334850	1.0828549	12.387276	1
ACGTC	345300	1.0803204	12.2631445	15
CAGTC	340980	1.0668046	12.00393	27
AGTCA	473955	0.99817234	8.312861	28
AATCT	718405	0.9771181	5.5956855	39
TTGTA	649375	0.9053115	5.665728	35
ACCTT	431865	0.8725962	7.8115163	32
TCGTA	404470	0.8447253	8.086403	43
TGTAA	551400	0.77519035	5.5556626	36
TATGC	357815	0.7472877	7.9495163	46
GTATG	338065	0.72978234	8.188311	45
CGTAT	343175	0.7167123	7.9286346	44
GTAAT	468690	0.6589118	5.47432	37
TAATC	463775	0.6307904	5.300672	38
>>END_MODULE