##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW240_GGCTAC_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4925928	
Filtered Sequences	0	
Sequence length	51	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.756325102599956	34.0	31.0	34.0	30.0	34.0
2	32.07118800762009	34.0	31.0	34.0	30.0	34.0
3	32.467628231675334	34.0	31.0	34.0	30.0	34.0
4	36.10113546117605	37.0	35.0	37.0	35.0	37.0
5	36.079204365147035	37.0	35.0	37.0	35.0	37.0
6	36.09572815518213	37.0	36.0	37.0	35.0	37.0
7	36.09482578714102	37.0	36.0	37.0	35.0	37.0
8	36.08972542838629	37.0	35.0	37.0	35.0	37.0
9	37.61453699688668	39.0	38.0	39.0	35.0	39.0
10	37.73036288796751	39.0	38.0	39.0	35.0	39.0
11	37.77126035946932	39.0	38.0	39.0	35.0	39.0
12	37.755376042849186	39.0	38.0	39.0	35.0	39.0
13	37.74392398752072	39.0	38.0	39.0	35.0	39.0
14	39.20253726810461	40.0	39.0	41.0	36.0	41.0
15	39.16488304335751	40.0	39.0	41.0	36.0	41.0
16	39.1516997406377	40.0	39.0	41.0	36.0	41.0
17	39.124771007615216	40.0	39.0	41.0	36.0	41.0
18	39.09347294560538	40.0	38.0	41.0	36.0	41.0
19	39.126697751164855	40.0	39.0	41.0	36.0	41.0
20	39.09500991488304	40.0	39.0	41.0	36.0	41.0
21	39.04500207879612	40.0	39.0	41.0	36.0	41.0
22	39.001846352606044	40.0	39.0	41.0	36.0	41.0
23	38.9315938032387	40.0	38.0	41.0	35.0	41.0
24	38.83135421386589	40.0	38.0	41.0	35.0	41.0
25	38.80889042633185	40.0	38.0	41.0	35.0	41.0
26	38.69491332394627	40.0	38.0	41.0	35.0	41.0
27	38.59119154806972	40.0	38.0	41.0	34.0	41.0
28	38.51405501663849	40.0	38.0	41.0	34.0	41.0
29	38.20680428134557	40.0	38.0	41.0	34.0	41.0
30	38.20072664480683	40.0	38.0	41.0	34.0	41.0
31	38.43242572770045	40.0	38.0	41.0	34.0	41.0
32	38.39775693026776	40.0	38.0	41.0	34.0	41.0
33	38.41497459970994	40.0	38.0	41.0	34.0	41.0
34	38.54983142262737	40.0	38.0	41.0	34.0	41.0
35	38.598911108729155	40.0	38.0	41.0	35.0	41.0
36	38.60200900216162	40.0	38.0	41.0	35.0	41.0
37	38.55979137332093	40.0	38.0	41.0	34.0	41.0
38	38.50555103525671	40.0	38.0	41.0	34.0	41.0
39	38.42277373928324	40.0	38.0	41.0	34.0	41.0
40	38.36550534234362	40.0	38.0	41.0	34.0	41.0
41	38.354202294471214	40.0	38.0	41.0	34.0	41.0
42	38.29522213885384	40.0	38.0	41.0	34.0	41.0
43	38.246837956218606	40.0	38.0	41.0	33.0	41.0
44	38.166634591492205	40.0	38.0	41.0	33.0	41.0
45	38.04466427442707	40.0	38.0	41.0	33.0	41.0
46	37.98172872197888	40.0	37.0	41.0	33.0	41.0
47	37.901199530322	40.0	37.0	41.0	33.0	41.0
48	37.8508871830851	40.0	37.0	41.0	33.0	41.0
49	37.78525630094472	40.0	37.0	41.0	33.0	41.0
50	37.69441960986843	40.0	37.0	41.0	33.0	41.0
51	36.70767701030141	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	5.0
13	2.0
14	13.0
15	21.0
16	40.0
17	88.0
18	224.0
19	525.0
20	941.0
21	1570.0
22	2431.0
23	4222.0
24	6738.0
25	9993.0
26	14770.0
27	21152.0
28	28830.0
29	38592.0
30	50627.0
31	65950.0
32	85433.0
33	111055.0
34	148808.0
35	207841.0
36	310902.0
37	514847.0
38	1057374.0
39	2242205.0
40	720.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.715884332304448	28.065589792846435	31.71628263032683	19.502243244522287
2	19.393023768525957	30.780425852925923	31.323775468815406	18.502774909732707
3	18.88253340284308	30.260998536722422	32.27761347709508	18.578854583339425
4	19.119443077527727	29.810687448131603	31.20449588382128	19.865373590519393
5	19.83902322567443	30.385056379224384	30.965820044466746	18.81010035063444
6	20.05108479052069	30.71309609072646	30.061482831255347	19.174336287497503
7	19.1213919488876	31.609373908835046	30.433493952814576	18.835740189462776
8	18.900641665895236	31.7731197045511	30.364978944069016	18.961259685484645
9	19.800545075290426	30.705258403244063	30.416865867831934	19.07733065363358
10	18.82573192300009	31.543213786316	30.527323988495166	19.103730302188744
11	19.64535413428698	30.59557508757741	30.624239737162217	19.134831040973395
12	18.675384617883168	30.53254127953149	30.677163775028788	20.114910327556554
13	18.64613124674173	31.56858971548102	30.757250207473597	19.02802883030365
14	18.635798168385733	30.64360664630096	30.79330432763126	19.927290857682046
15	18.63072298255273	31.57790775667042	30.786625383075027	19.004743877701827
16	18.685920703672483	30.60507583545679	30.82548100581251	19.883522455058213
17	19.68226088566459	30.561002921682977	30.822050180189397	18.934686012463033
18	18.824331171710185	30.489260094747628	31.63326788373683	19.053140849805356
19	18.836640452109254	30.471332179835965	30.669428401655015	20.02259896639977
20	18.9022657253618	30.42265335587528	31.553932578795308	19.12114833996762
21	19.822155037881533	30.388848319779054	30.64588526703847	19.143111375300943
22	18.899251307369994	31.33394371000747	30.610627050378408	19.156177932244127
23	18.919802849738094	31.357472695728156	30.58322062712428	19.139503827409474
24	18.92316487964287	30.38080539103784	30.593758044855473	20.102271684463815
25	18.953975566426383	30.449903134983526	31.45895178714585	19.13716951144424
26	18.96408159253207	30.481376279935223	30.524776995062027	20.029765132470683
27	18.999750415855114	30.46177534543238	30.47294469284396	20.06552954586855
28	19.02520986554955	31.42113020276441	30.432799962300578	19.120859969385467
29	19.93247653522826	30.452874231520173	30.456939694877782	19.157709538373783
30	19.025447811855443	30.42219910143089	31.304903697751218	19.247449388962444
31	19.03459247697273	30.407916898793697	30.42610807834551	20.131382545888062
32	19.020397298782786	31.378984188715396	30.37140212760986	19.22921638489196
33	19.035689786051364	30.430261078503882	30.421772503759954	20.112276631684804
34	19.933390157921732	30.476083927670068	30.409770526393586	19.180755388014614
35	19.92212680158922	30.47435530164495	30.488912367882442	19.11460552888339
36	19.05367645177704	30.473480549235326	30.417420796564215	20.055422202423422
37	19.028259588386508	30.50118051214691	31.36263147997191	19.107928419494677
38	19.020342217793385	31.407607714491288	30.456384482557997	19.115665585157334
39	19.083781598998197	30.484650805598935	30.40763121403223	20.023936381370643
40	19.11655583575228	31.339101170964184	30.358518682180964	19.18582431110257
41	19.08049389193611	30.49471429067452	31.26949411793133	19.15529769945804
42	19.060149185118526	30.423250234320047	30.40838695214998	20.10821362841145
43	19.042478882391162	30.422981643924622	31.31028671215075	19.224252761533464
44	19.079361186245418	30.43108030570606	30.425253195063647	20.064305312984875
45	19.965677682047758	30.459930429599456	30.464965289916595	19.109426598436187
46	19.120012314620688	30.465973549559443	31.29635752040726	19.11765661541261
47	19.044388821254916	31.428667726250524	30.446474907435306	19.08046854505925
48	19.089256183245165	30.495035232036027	31.291123922760576	19.12458466195823
49	20.020458148001584	30.447555488868726	30.392856947070122	19.139129416059564
50	19.12771982231232	30.417990004560362	30.365523489526797	20.088766683600518
51	19.148558169452652	30.375921194332413	30.35169847362343	20.12382216259151
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	40.21812757682673
2	37.89579867825867
3	37.4613879861825
4	38.98481666804712
5	38.64912357630887
6	39.225421078018194
7	37.95713213835038
8	37.861901351379885
9	38.87787572892401
10	37.929462225188836
11	38.78018517526038
12	38.790294945439726
13	37.674160077045386
14	38.56308902606778
15	37.63546686025456
16	38.5694431587307
17	38.61694689812762
18	37.87747202151554
19	38.85923941850902
20	38.02341406532941
21	38.965266413182476
22	38.05542923961413
23	38.05930667714756
24	39.025436564106684
25	38.09114507787062
26	38.993846725002754
27	39.06527996172366
28	38.14606983493501
29	39.09018607360205
30	38.27289720081789
31	39.16597502286079
32	38.249613683674745
33	39.14796641773617
34	39.114145545936346
35	39.03673233047261
36	39.109098654200466
37	38.13618800788118
38	38.136007802950715
39	39.10771798036884
40	38.30238014685485
41	38.235791591394154
42	39.168362813529974
43	38.26673164392463
44	39.14366649923029
45	39.07510428048395
46	38.237668930033294
47	38.12485736631417
48	38.21384084520339
49	39.15958756406115
50	39.21648650591284
51	39.27238033204416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	30.0
2	25.0
3	67.5
4	110.0
5	226.0
6	342.0
7	740.0
8	1138.0
9	2174.0
10	3210.0
11	5680.5
12	8151.0
13	12713.0
14	17275.0
15	24832.5
16	32390.0
17	43615.0
18	54840.0
19	70643.5
20	86447.0
21	105793.5
22	125140.0
23	146735.5
24	168331.0
25	188500.0
26	227651.0
27	246633.0
28	262401.0
29	278169.0
30	289982.5
31	301796.0
32	308387.0
33	314978.0
34	320501.0
35	326024.0
36	327293.0
37	328562.0
38	326265.5
39	323969.0
40	318027.5
41	312086.0
42	302720.5
43	293355.0
44	280418.5
45	267482.0
46	250775.5
47	234069.0
48	217107.0
49	200145.0
50	185093.5
51	170042.0
52	155784.5
53	141527.0
54	145874.0
55	150221.0
56	119499.0
57	88777.0
58	78724.5
59	68672.0
60	60576.5
61	52481.0
62	45404.5
63	38328.0
64	32763.5
65	27199.0
66	23119.0
67	19039.0
68	16021.5
69	13004.0
70	10849.5
71	8695.0
72	7164.0
73	5633.0
74	4644.5
75	2965.0
76	2274.0
77	1815.0
78	1356.0
79	1094.5
80	833.0
81	627.5
82	422.0
83	327.0
84	232.0
85	164.0
86	96.0
87	66.0
88	36.0
89	25.0
90	14.0
91	11.5
92	9.0
93	7.5
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05032554272007224
2	4.060148666403569E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.0902229996053534E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	7.917289899486959E-4
20	0.0
21	0.0035932315697671585
22	1.6240594665614276E-4
23	0.002882705553146534
24	0.008526312199447494
25	2.0300743332017845E-5
26	0.04153532085730851
27	0.035262391167715
28	0.056354863489681536
29	0.6300741707958379
30	0.06069922256273336
31	0.00941954490605628
32	0.09019620262415529
33	0.033739835417813654
34	0.01684961696557481
35	0.009845860516028655
36	0.016768413992246742
37	0.03641953353764001
38	0.03323231683451321
39	0.024502997201745542
40	0.03268419676454873
41	0.021214276781958648
42	0.02111277306529856
43	0.02289923847851613
44	0.013682701005780028
45	0.005481200699644818
46	0.03451126366443034
47	0.01469773817238092
48	0.014677437429048902
49	0.015245858242345402
50	0.017905255618839738
51	0.016240594665614276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4925928.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	16.964325152019317
#Duplication Level	Relative count
1	100.0
2	13.646706976139393
3	1.9398194939447133
4	0.6761588973408751
5	0.3977752953762316
6	0.267153690208108
7	0.19859212369452275
8	0.16076505251461365
9	0.12707531724500712
10++	0.7955505907524631
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC	38320	0.7779244844829238	TruSeq Adapter, Index 11 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2383190	3.7987845	3.9665384	11
TTTTT	2395505	3.7648962	4.1347685	1
GAAAA	1194575	3.0409703	3.1347685	9
TTTTC	1214090	3.0283144	3.2769675	1
TCCAG	304260	1.9344337	15.059863	25
CTGAA	470895	1.8917553	10.303328	19
GGAAG	290705	1.8874564	15.575841	5
CTCCA	293395	1.8484895	14.739805	24
GAGCA	281090	1.8085276	15.296036	9
CGGAA	280780	1.8065331	15.385144	4
GAAGA	442675	1.7996855	10.241713	6
ACGGC	151685	1.544509	21.902369	32
CGGCT	150615	1.5292907	21.799501	33
TCTGA	365255	1.4632248	9.743522	18
TCTCG	225575	1.4301249	14.093766	41
GCACA	221600	1.4128785	14.774506	11
CACAC	218740	1.3820333	14.596414	12
CCAGT	216560	1.376852	14.42899	26
CACGG	135180	1.3764495	21.88615	31
TCGGA	214225	1.3744339	14.980257	3
AGAGC	211805	1.3627491	14.830779	8
AGCAC	212745	1.3564208	14.743071	10
ATCGG	200060	1.2835535	14.602174	2
TGAAC	317520	1.2755927	9.59655	20
AAGAG	303525	1.2339741	9.7616005	7
GTCTG	191750	1.2267697	14.581246	17
ATGCC	191585	1.2180651	13.7540865	47
GTCAC	190870	1.2135192	14.125583	29
ACTCC	184385	1.161689	14.179068	23
AACTC	285935	1.1383178	9.396582	22
GAACT	281040	1.1290392	9.457878	21
CGTCT	177855	1.1275845	14.32822	16
CACGT	176070	1.1194234	14.397	14
CTCGT	174685	1.1074868	13.688607	42
CATCT	278180	1.1043229	8.915043	39
TCACG	172340	1.0957085	13.984537	30
GATCG	169200	1.0855606	14.279407	1
ATCTC	270415	1.0734973	8.99228	40
CAGTC	166685	1.0597552	14.050466	27
ACACG	166175	1.0594994	14.317947	13
CTACA	256445	1.0209169	8.836674	36
ACGTC	159590	1.0146464	14.31499	15
ACATC	247005	0.9833361	8.750539	38
AGTCA	240045	0.96434754	9.15341	28
GCTAC	142685	0.90716714	13.386336	35
GGCTA	126660	0.81263053	13.549878	34
TATGC	186270	0.7462044	8.6291895	46
TCGTA	176220	0.70594364	8.601538	43
GTATG	174060	0.7036531	8.672681	45
TACAT	274095	0.6875482	5.6124277	37
CGTAT	160980	0.6448917	8.532929	44
>>END_MODULE