##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW238_GATCAG_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5522722	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67812900957173	34.0	31.0	34.0	30.0	34.0
2	31.997479865906705	34.0	31.0	34.0	30.0	34.0
3	32.42654419324384	34.0	31.0	34.0	30.0	34.0
4	36.08039332778293	37.0	35.0	37.0	35.0	37.0
5	36.05986486373929	37.0	35.0	37.0	35.0	37.0
6	36.07452701765543	37.0	35.0	37.0	35.0	37.0
7	36.07102367274688	37.0	35.0	37.0	35.0	37.0
8	36.06880393400211	37.0	35.0	37.0	35.0	37.0
9	37.60594485835065	39.0	38.0	39.0	35.0	39.0
10	37.70951190373153	39.0	38.0	39.0	35.0	39.0
11	37.74934715164008	39.0	38.0	39.0	35.0	39.0
12	37.73124176809914	39.0	38.0	39.0	35.0	39.0
13	37.710834439973624	39.0	38.0	39.0	35.0	39.0
14	39.16814606999954	40.0	39.0	41.0	36.0	41.0
15	39.109796038982225	40.0	39.0	41.0	36.0	41.0
16	39.11565782235644	40.0	39.0	41.0	36.0	41.0
17	39.08273800491859	40.0	38.0	41.0	36.0	41.0
18	39.0509082296737	40.0	38.0	41.0	36.0	41.0
19	39.0787006841916	40.0	39.0	41.0	36.0	41.0
20	39.03937225158174	40.0	39.0	41.0	36.0	41.0
21	38.98419131000981	40.0	38.0	41.0	36.0	41.0
22	38.91331249336831	40.0	38.0	41.0	35.0	41.0
23	38.83871395301085	40.0	38.0	41.0	35.0	41.0
24	38.75067928459915	40.0	38.0	41.0	35.0	41.0
25	38.719922168814584	40.0	38.0	41.0	35.0	41.0
26	38.612730099396636	40.0	38.0	41.0	34.0	41.0
27	38.505612811943095	40.0	38.0	41.0	34.0	41.0
28	38.36328716165688	40.0	38.0	41.0	34.0	41.0
29	38.08285207910157	40.0	38.0	41.0	34.0	41.0
30	38.03448480658632	40.0	38.0	41.0	33.0	41.0
31	38.28518400889996	40.0	38.0	41.0	34.0	41.0
32	38.23040631051138	40.0	38.0	41.0	34.0	41.0
33	38.25844592575908	40.0	38.0	41.0	34.0	41.0
34	38.39934275163588	40.0	38.0	41.0	34.0	41.0
35	38.40075763364515	40.0	38.0	41.0	34.0	41.0
36	38.43109557207478	40.0	38.0	41.0	34.0	41.0
37	38.36047514251125	40.0	38.0	41.0	34.0	41.0
38	38.30362980428854	40.0	38.0	41.0	34.0	41.0
39	38.219670843471754	40.0	38.0	41.0	33.0	41.0
40	38.126223264542375	40.0	38.0	41.0	33.0	41.0
41	38.107524695249914	40.0	38.0	41.0	33.0	41.0
42	38.05143767873886	40.0	38.0	41.0	33.0	41.0
43	37.98982150468555	40.0	37.0	41.0	33.0	41.0
44	37.901760037894356	40.0	37.0	41.0	33.0	41.0
45	37.76405040847611	40.0	37.0	41.0	33.0	41.0
46	37.66226907673426	40.0	37.0	41.0	33.0	41.0
47	37.56737003962901	40.0	37.0	41.0	32.0	41.0
48	37.50585671341052	40.0	36.0	41.0	32.0	41.0
49	37.42864533105233	40.0	36.0	41.0	32.0	41.0
50	37.32076012517016	39.0	36.0	41.0	32.0	41.0
51	36.30073901963561	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	4.0
12	11.0
13	12.0
14	11.0
15	28.0
16	67.0
17	133.0
18	344.0
19	743.0
20	1407.0
21	2216.0
22	3838.0
23	6000.0
24	9150.0
25	13608.0
26	19273.0
27	26563.0
28	35826.0
29	47938.0
30	62517.0
31	79565.0
32	102107.0
33	132689.0
34	177597.0
35	249039.0
36	376740.0
37	614926.0
38	1195657.0
39	2363953.0
40	756.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.79723105512401	27.198296546735456	30.577286818798495	21.42718557934204
2	19.660935068565877	30.286246735755835	30.11600447460834	19.936813721069953
3	19.623566060359366	28.78254599815091	31.807775223884168	19.78611271760556
4	19.62479733725507	28.348774390599417	29.85891739616805	22.167510875977463
5	21.517939885440548	29.232487168465116	29.71056663724881	19.53900630884553
6	21.776326963406813	29.586714667151455	28.73434512908671	19.902613240355027
7	19.876991816716465	31.45780287329328	29.350725964479107	19.314479345511145
8	19.425475336256287	31.469029221459998	29.48509810922947	19.620397333054242
9	21.483012799678132	29.22329715609183	29.526752864577343	19.766937179652697
10	19.64531258317909	31.19655488724582	29.293924264158143	19.864208265416945
11	21.61530129526708	29.156256643010458	29.24641146159448	19.98203060012798
12	19.650020406603844	29.172824560062953	29.22868469569897	21.948470337634234
13	19.63602368542179	31.191991919926444	29.217404026492733	19.954580368159032
14	19.577682888981194	29.34601814105436	29.283295447426106	21.793003522538342
15	19.569534733053736	31.28484468347311	29.289614795023176	19.85600578844997
16	19.5992483416692	29.324217297195116	29.336946527455122	21.739587833680567
17	21.590838720471535	29.296477353015415	29.26801312830883	19.84467079820422
18	19.722647636437248	29.129621226634256	31.180620715654346	19.96711042127415
19	19.74293254627513	29.137655212374437	29.232609197535613	21.88680304381482
20	19.812186816573423	29.104832725601614	31.104209120067967	19.978771337757
21	21.76252007012025	29.028215958930293	29.133711410004832	20.07555256094463
22	19.81077788537379	31.01162543936715	29.133219959165018	20.044376716094035
23	19.785627200871193	31.011480428622466	29.197872931043005	20.005019439463332
24	19.824306516695877	29.047553425418098	29.19012261529806	21.938017442587967
25	19.8287221099889	29.118327722874287	31.028110961969652	20.024839205167165
26	19.797266857701505	29.17646884072502	29.11154670291003	21.914717598663444
27	19.86231756080552	29.134680088938396	29.08100911573313	21.92199323452295
28	19.875399082025282	31.081401319167423	29.06154170038021	19.98165789842708
29	21.82070766060775	29.13864035664736	29.04221175007717	19.998440232667726
30	20.010392693805805	29.00407699435178	30.87112008815671	20.114410223685702
31	19.95925820893306	29.04295001135439	28.976127534228862	22.021664245483688
32	19.92027049167255	30.926937090677637	29.01322590607316	20.139566511576653
33	19.964063211796105	29.036345243130306	29.024951977112035	21.974639567961557
34	21.819307282586117	29.123335834347046	29.018387431111197	20.038969451955634
35	19.963333248221847	30.994560289625326	29.00785904958722	20.034247412565605
36	19.931800223807112	29.143116482385285	30.89342286342351	20.031660430384086
37	19.908402073741694	29.190227453330213	29.042491199857118	21.858879273070976
38	19.94616102028163	31.026061478666456	29.024107814168943	20.003669686882972
39	21.877623378836777	29.139308060993972	28.933069166914045	20.04999939325521
40	19.99932255973882	30.946195665758946	28.99716018491583	20.0573215895864
41	19.974561205240327	29.112098043136196	30.808452359219107	20.104888392404366
42	19.984319589158023	29.018014723793495	29.01022703996114	21.987438647087345
43	19.93852980462294	28.98006384279278	30.916809671503927	20.164596681080347
44	19.938673971316717	29.043426955853736	29.042249838010477	21.975649234819073
45	21.892082662430838	29.062464010291155	29.002798052734928	20.04265527454308
46	19.984143550678148	29.153007671739257	30.873307440866167	19.989541336716428
47	19.92261749536393	31.081707087389894	29.01555980528512	19.980115611961057
48	19.985244273553928	29.126949230014752	30.892366501241238	19.995439995190083
49	21.857343385792362	29.11558535000968	28.978964050524525	20.04810721367343
50	20.03543106589483	29.064756774534256	28.941660804953827	21.958151354617087
51	20.03241702418962	28.98963922930789	28.951771074234983	22.026172672267506
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.22441663446605
2	39.59774878963583
3	39.40967877796492
4	41.79230821323253
5	41.05694619428608
6	41.67894020376184
7	39.19147116222761
8	39.04587266931053
9	41.24994997933082
10	39.50952084859603
11	41.597331895395065
12	41.59849074423808
13	39.590604053580826
14	41.370686411519536
15	39.42554052150371
16	41.33883617534976
17	41.43550951867576
18	39.689758057711394
19	41.62973559008994
20	39.79095815433042
21	41.83807263106488
22	39.85515460146783
23	39.79064664033453
24	41.76232395928385
25	39.853561315156064
26	41.71198445636495
27	41.78431079532847
28	39.85705698045236
29	41.81914789327547
30	40.1248029174915
31	41.98092245441675
32	40.05983700324921
33	41.93870277975766
34	41.85827673454175
35	39.997580660787456
36	39.9634606541912
37	41.767281346812666
38	39.9498307071646
39	41.927622772091986
40	40.05664414932522
41	40.0794495976447
42	41.971758236245364
43	40.103126485703285
44	41.91432320613579
45	41.93473793697392
46	39.973684887394576
47	39.90273310732499
48	39.98068426874401
49	41.905450599465794
50	41.99358242051191
51	42.058589696457126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	38.5
2	24.0
3	65.5
4	107.0
5	249.0
6	391.0
7	844.0
8	1297.0
9	2444.5
10	3592.0
11	6161.0
12	8730.0
13	13341.0
14	17952.0
15	25496.5
16	33041.0
17	44189.0
18	55337.0
19	71036.5
20	86736.0
21	104800.0
22	122864.0
23	143295.0
24	163726.0
25	183528.5
26	221528.5
27	239726.0
28	256111.0
29	272496.0
30	284931.5
31	297367.0
32	303210.0
33	309053.0
34	315868.0
35	322683.0
36	323125.0
37	323567.0
38	321701.5
39	319836.0
40	316160.0
41	312484.0
42	307341.0
43	302198.0
44	294431.0
45	286664.0
46	275814.5
47	264965.0
48	253280.5
49	241596.0
50	233177.5
51	224759.0
52	256057.0
53	287355.0
54	227977.0
55	168599.0
56	154427.0
57	140255.0
58	128373.5
59	116492.0
60	105992.0
61	95492.0
62	85828.5
63	76165.0
64	68747.5
65	61330.0
66	54463.5
67	47597.0
68	42099.5
69	36602.0
70	31934.0
71	27266.0
72	23310.0
73	19354.0
74	16200.0
75	10681.0
76	8316.0
77	6646.5
78	4977.0
79	3879.5
80	2782.0
81	2082.5
82	1383.0
83	1017.5
84	652.0
85	458.0
86	264.0
87	203.0
88	142.0
89	92.0
90	42.0
91	30.0
92	18.0
93	14.0
94	10.0
95	5.5
96	1.0
97	4.0
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04782062178758952
2	7.242805268851122E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	9.053506586063902E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	8.510296190900067E-4
20	0.0
21	0.0037843657529747104
22	1.086420790327668E-4
23	0.003494653542220666
24	0.008890543467514752
25	1.8107013172127805E-5
26	0.04097617080852522
27	0.03706505596334561
28	0.05758030188736641
29	0.6287479253889658
30	0.06265026557556219
31	0.01137120427209626
32	0.09173012872999944
33	0.034711144250968995
34	0.01740083965841482
35	0.010013178284186675
36	0.018161334211644186
37	0.03753583830582093
38	0.03286422890741196
39	0.026345704165445953
40	0.03512760555392794
41	0.022344054254405707
42	0.02142059658262719
43	0.02439014674285615
44	0.01376133001081713
45	0.005613174083359618
46	0.03512760555392794
47	0.014521824564046496
48	0.014449396511357987
49	0.016097134710021613
50	0.01836051135653759
51	0.016875736276423112
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5522722.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.653789471141064
#Duplication Level	Relative count
1	100.0
2	26.567984339045918
3	5.57475014596284
4	1.5468626575540063
5	0.7631280695126559
6	0.513102311364495
7	0.4286155853968472
8	0.2932994470584195
9	0.2335405433252052
10++	1.4555070920767936
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC	92120	1.6680180534164133	TruSeq Adapter, Index 9 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	2434925	4.159459	4.435069	1
AAAAA	2428080	4.115898	4.2806735	13
TTTTC	1275780	3.1316178	3.472819	2
GAAAA	1250850	3.09856	3.2789853	4
ATTTT	1766430	3.0128522	3.181923	14
TCCAG	441130	2.2668066	27.171902	25
GGAAG	382260	2.0221803	28.320024	5
CTGAA	563665	2.0125992	19.568947	19
CGGAA	379505	1.9771382	27.930597	4
GAAGA	525965	1.90399	19.77291	6
GAGCA	362465	1.8883631	27.61502	9
CTCCA	365585	1.8500978	26.440855	24
TCTCG	328740	1.6918833	26.384567	41
CCAGT	312200	1.6042823	26.456787	26
ATCGG	302980	1.5808964	26.977785	2
TCTGA	439635	1.5721663	19.20881	18
TCAGA	437825	1.5632801	18.422213	36
GCACA	301335	1.5460645	26.894785	11
AGCAC	299010	1.5341353	26.899601	10
GATCG	293445	1.5311445	26.771372	1
AGAGC	290975	1.515916	27.260307	8
TCGGA	289930	1.5128038	27.492273	3
ATGCC	293490	1.5081385	26.129807	47
CACAC	296010	1.4956945	26.392902	12
CGATC	291065	1.4956774	25.783178	33
GTCTG	282795	1.4778526	27.148018	17
TGAAC	402095	1.4357042	19.007	20
GTCAC	275395	1.4151549	26.30267	29
CACGA	272300	1.397094	25.90282	31
TCACG	267355	1.3738401	26.19323	30
ATCAG	384645	1.373398	18.169521	35
CGTCT	264660	1.3620913	26.705053	16
AAGAG	374935	1.3572625	19.217926	7
GAACT	372510	1.3300691	18.84099	21
CAGAT	364825	1.3026294	18.113504	37
GATCA	364525	1.3015581	18.16151	34
CACGT	253200	1.3011029	26.6178	14
CTCGT	252590	1.2999719	25.821653	42
ACTCC	254150	1.2861645	25.998783	23
ACGTC	249585	1.2825266	26.609596	15
CAGTC	246405	1.2661858	26.12655	27
AACTC	355585	1.2503691	18.463417	22
ATCTC	354335	1.2478971	18.109608	40
ACACG	240090	1.2318336	26.486427	13
AGATC	321905	1.1493809	18.154306	38
GATCT	317815	1.1365292	18.182467	39
AGTCA	313045	1.1177458	18.33194	28
ACGAT	307770	1.098911	18.119574	32
TATGC	262685	0.93938047	18.013083	46
GTATG	244480	0.8877508	18.255466	45
TCGTA	244640	0.8748502	18.011305	43
CGTAT	235050	0.8405557	17.97974	44
>>END_MODULE