##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW237_ACTTGA_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5155376	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.687442584207243	34.0	31.0	34.0	30.0	34.0
2	32.0045116010937	34.0	31.0	34.0	30.0	34.0
3	32.43046423772	34.0	31.0	34.0	30.0	34.0
4	36.080717875863954	37.0	35.0	37.0	35.0	37.0
5	36.05994538516686	37.0	35.0	37.0	35.0	37.0
6	36.07507231286331	37.0	35.0	37.0	35.0	37.0
7	36.06247672332726	37.0	35.0	37.0	35.0	37.0
8	36.060148086191965	37.0	35.0	37.0	35.0	37.0
9	37.601337516410055	39.0	38.0	39.0	35.0	39.0
10	37.694906637265646	39.0	38.0	39.0	35.0	39.0
11	37.741338168156894	39.0	38.0	39.0	35.0	39.0
12	37.7201951904187	39.0	38.0	39.0	35.0	39.0
13	37.70602512794411	39.0	37.0	39.0	35.0	39.0
14	39.16505702008932	40.0	39.0	41.0	36.0	41.0
15	39.113169631080254	40.0	39.0	41.0	36.0	41.0
16	39.10723970472765	40.0	39.0	41.0	36.0	41.0
17	39.075355318409365	40.0	38.0	41.0	36.0	41.0
18	39.03649627107703	40.0	38.0	41.0	36.0	41.0
19	39.073261581696464	40.0	39.0	41.0	36.0	41.0
20	39.03129839608207	40.0	39.0	41.0	36.0	41.0
21	38.98269709134698	40.0	38.0	41.0	36.0	41.0
22	38.91197654642455	40.0	38.0	41.0	35.0	41.0
23	38.85221465902778	40.0	38.0	41.0	35.0	41.0
24	38.75209218493472	40.0	38.0	41.0	35.0	41.0
25	38.72333754123851	40.0	38.0	41.0	35.0	41.0
26	38.60628788278488	40.0	38.0	41.0	34.0	41.0
27	38.49163688545705	40.0	38.0	41.0	34.0	41.0
28	38.37333629981596	40.0	38.0	41.0	34.0	41.0
29	38.078745759766115	40.0	38.0	41.0	34.0	41.0
30	38.02435283090894	40.0	38.0	41.0	33.0	41.0
31	38.28180815521506	40.0	38.0	41.0	34.0	41.0
32	38.22531799814407	40.0	38.0	41.0	34.0	41.0
33	38.25870683341041	40.0	38.0	41.0	34.0	41.0
34	38.39337790298904	40.0	38.0	41.0	34.0	41.0
35	38.45739883958027	40.0	38.0	41.0	34.0	41.0
36	38.450401483810296	40.0	38.0	41.0	34.0	41.0
37	38.39905547141469	40.0	38.0	41.0	34.0	41.0
38	38.320382451250886	40.0	38.0	41.0	34.0	41.0
39	38.18666611319912	40.0	38.0	41.0	33.0	41.0
40	38.130057051124886	40.0	38.0	41.0	33.0	41.0
41	38.12333222639823	40.0	38.0	41.0	33.0	41.0
42	38.060287746228404	40.0	38.0	41.0	33.0	41.0
43	37.99362471330898	40.0	37.0	41.0	33.0	41.0
44	37.9051396057242	40.0	37.0	41.0	33.0	41.0
45	37.74228300709783	40.0	37.0	41.0	33.0	41.0
46	37.63275714516264	40.0	37.0	41.0	33.0	41.0
47	37.538579145342645	40.0	37.0	41.0	32.0	41.0
48	37.4870564242065	40.0	36.0	41.0	32.0	41.0
49	37.40293899028897	40.0	36.0	41.0	32.0	41.0
50	37.30459000468637	39.0	36.0	41.0	32.0	41.0
51	36.29581314728547	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	5.0
12	4.0
13	9.0
14	16.0
15	21.0
16	70.0
17	142.0
18	289.0
19	695.0
20	1337.0
21	2043.0
22	3459.0
23	5460.0
24	8583.0
25	12500.0
26	17769.0
27	24618.0
28	33501.0
29	45181.0
30	58029.0
31	74711.0
32	95635.0
33	124101.0
34	166041.0
35	232969.0
36	351462.0
37	576160.0
38	1119058.0
39	2200848.0
40	654.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.507802758983384	27.08870683691103	30.11582606974404	22.28766433436154
2	19.577528307834058	30.62683121984337	30.43660257498242	19.359037897340155
3	19.68058197888961	28.801546191781163	31.85831644481411	19.659555384515116
4	19.505541399890134	27.986280729087458	29.906354066124376	22.601823804898032
5	21.88503806511882	28.766980332763314	29.900496103484986	19.447485498632883
6	22.06486975925713	29.273655306615854	28.6353701456499	20.026104788477117
7	20.06069004472225	31.564545437616964	29.205396463807876	19.16936805385291
8	19.29384006132627	31.63877862642802	29.511193751920327	19.556187560325377
9	21.71299729427919	28.891760111247045	29.672149813408268	19.723092781065503
10	19.61383611980969	31.262899156143025	29.283315125802655	19.839949598244626
11	21.888898113348084	29.00341313611267	29.14951305200629	19.958175698532948
12	19.65266936882974	28.99739999565502	29.073572907194357	22.276357728320882
13	19.5871843295232	31.447114623647238	29.05557228027597	19.910128766553594
14	19.544762593455843	29.226811002728027	29.107013727029802	22.121412676786328
15	19.50848977843711	31.563439795661846	29.137758332272952	19.79031209362809
16	19.492040929701346	29.23984593946203	29.165981297969346	22.102131832867283
17	21.887695485256558	29.162334619240188	29.20194375735155	19.748026138151708
18	19.697806716716688	28.98488878405765	31.424322881590015	19.892981617635648
19	19.660099683143773	28.987621560965486	29.100844077058035	22.251434678832705
20	19.731965233961596	28.97695531809901	31.347684436595895	19.943395011343497
21	22.062887401453526	28.889823870427854	29.070574035989416	19.976714692129203
22	19.725424009580696	31.2850084193812	29.026798674080815	19.962768896957286
23	19.781897804895177	31.265181279167166	29.00707208530109	19.945848830636574
24	19.795239917818176	28.95260461334832	29.021703769473422	22.230451699360085
25	19.757996312975038	29.02969637908079	31.262142664278997	19.950164643665175
26	19.754987460033952	28.994897076026117	29.044342810303032	22.205772653636902
27	19.798477217839192	28.956573270550944	28.996488186000015	22.248461325609853
28	19.814255126218477	31.286682390278898	28.93702972787489	19.96203275562774
29	22.097098484434415	28.995459549780982	28.934516791467235	19.97292517431737
30	19.956829471950027	28.833068135367807	31.121463256139307	20.088639136542856
31	19.87696649528134	28.90294122668024	28.87062218614836	22.349470091890055
32	19.878934328532658	31.170702091972714	28.88294609960445	20.067417479890178
33	19.961610719814527	28.927149238908846	28.827450430986808	22.283789610289816
34	19.879716402317595	31.235516254291166	28.85467704339037	20.03009030000087
35	19.839017374344355	29.028881971552185	28.95681380729031	22.17528684681315
36	19.899045632398522	29.006958704083964	31.092688044873434	20.00130761864408
37	19.88120441107698	29.013309685469174	31.13366153153555	19.971824371918288
38	22.100335590329486	29.08348621984029	28.848718518396343	19.96745967143388
39	19.906116348750952	31.22780017768589	28.845746025592746	20.02033744797042
40	19.90497885939704	31.237292838584363	28.847754671985935	20.009973630032658
41	19.910542927676126	28.984068150969577	31.07081132217506	20.034577599179233
42	19.959055085112418	28.896256714372353	28.875341902844298	22.26934629767093
43	19.93664537513567	28.91337136861434	31.058414539550753	20.091568716699243
44	19.912820361600144	28.96785888608408	28.865717981687972	22.253602770627808
45	22.139940590398673	29.006069571657665	28.87823398335499	19.975755854588666
46	19.93078897234004	29.03288901514578	31.06453189027914	19.971790122235042
47	19.95252018968644	31.280996513413616	28.889526505583735	19.876956791316204
48	19.9793971233573	28.955732915326443	31.11501914787123	19.949850813445025
49	22.11867354657372	28.914738442537985	28.90988833698999	20.056699673898304
50	19.983175558929585	28.876111039758996	28.860357527133097	22.280355874178323
51	19.986753242671554	28.817032365506517	28.854533803578992	22.34168058824294
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.795467093344925
2	38.93656620517421
3	39.34013736340473
4	42.10736520478817
5	41.33252356375171
6	42.09097454773425
7	39.23005809857516
8	38.85002762165165
9	41.436090075344694
10	39.45378571805432
11	41.84707381188104
12	41.92902709715062
13	39.497313096076795
14	41.66617527024217
15	39.2988018720652
16	41.594172762568626
17	41.63572162340826
18	39.59078833435233
19	41.91153436197648
20	39.6753602453051
21	42.03960209358273
22	39.688192906537985
23	39.727746635531744
24	42.02569161717826
25	39.708160956640214
26	41.96076011367085
27	42.046938543449045
28	39.776287881846216
29	42.070023658751786
30	40.04546860849288
31	42.22643658717139
32	39.94635180842284
33	42.245400330104346
34	39.90980670231847
35	42.0143042211575
36	39.9003532510426
37	39.85302878299527
38	42.06779526176337
39	39.92645379672137
40	39.9149524894297
41	39.94512052685536
42	42.228401382783346
43	40.028214091834904
44	42.16642313222795
45	42.11569644498734
46	39.90257909457508
47	39.82947698100264
48	39.92924793680233
49	42.175373220472025
50	42.26353143310791
51	42.32843383091449
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	92.0
1	63.5
2	35.0
3	83.5
4	132.0
5	260.0
6	388.0
7	800.5
8	1213.0
9	2296.0
10	3379.0
11	5779.5
12	8180.0
13	12252.0
14	16324.0
15	23287.0
16	30250.0
17	40526.5
18	50803.0
19	64729.0
20	78655.0
21	95190.0
22	111725.0
23	130868.5
24	150012.0
25	168180.0
26	203772.5
27	221197.0
28	236060.5
29	250924.0
30	263030.5
31	275137.0
32	280581.5
33	286026.0
34	292682.0
35	299338.0
36	300624.5
37	301911.0
38	300056.5
39	298202.0
40	296034.0
41	293866.0
42	289345.0
43	284824.0
44	277482.0
45	270140.0
46	260469.0
47	250798.0
48	243000.0
49	235202.0
50	274876.5
51	314551.0
52	249009.0
53	183467.0
54	168550.5
55	153634.0
56	141593.5
57	129553.0
58	118975.5
59	108398.0
60	98342.5
61	88287.0
62	79255.0
63	70223.0
64	62657.5
65	55092.0
66	48984.0
67	42876.0
68	37965.5
69	33055.0
70	28794.0
71	24533.0
72	20990.0
73	17447.0
74	14767.0
75	9847.5
76	7608.0
77	6106.5
78	4605.0
79	3576.0
80	2547.0
81	1892.0
82	1237.0
83	911.0
84	585.0
85	429.5
86	274.0
87	195.5
88	117.0
89	86.5
90	56.0
91	40.0
92	24.0
93	18.5
94	13.0
95	9.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04637877043303922
2	1.9397227282743296E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	5.819168184822989E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	7.952863185924752E-4
20	0.0
21	0.0037630620928521996
22	1.7457504554468966E-4
23	0.0034527064563283066
24	0.009019710686475632
25	0.0
26	0.0436825558407379
27	0.03712629301917067
28	0.05896757093953962
29	0.6311081868713359
30	0.0630409886689157
31	0.010086558187026513
32	0.09277693809336118
33	0.03596245938220607
34	0.017282929508924277
35	0.01035811936898492
36	0.01877651600969551
37	0.038522893383528184
38	0.03435248951773838
39	0.025934092877027786
40	0.03464344792697952
41	0.022733550375375142
42	0.021996455738630898
43	0.023548233921250362
44	0.014198770370968094
45	0.0061489210486296245
46	0.03611763720046802
47	0.01481948164401588
48	0.014664303825753932
49	0.016468245963049057
50	0.018815310464260996
51	0.017593285145448168
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5155376.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	24.290353040931166
#Duplication Level	Relative count
1	100.0
2	19.514600792878095
3	3.480136685565928
4	1.0773215228859236
5	0.6497018752903295
6	0.47725407097722544
7	0.349986318716632
8	0.24562676186294535
9	0.18390190201655754
10++	1.2892223303998116
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC	102566	1.989496013481849	TruSeq Adapter, Index 8 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2234230	4.0900135	4.313735	14
TTTTT	2238060	4.0882597	4.3787003	2
TTTTC	1170410	3.0635164	3.4271693	2
GAAAA	1145200	3.063002	3.199358	13
TCCAG	422185	2.3154826	31.351715	25
CACAC	410125	2.2059786	30.614288	12
TTGAA	801460	2.141784	16.006021	36
GGAAG	370265	2.1140566	32.909847	5
CGGAA	368545	2.0627894	32.43377	4
CTGAA	537125	2.056765	22.584204	19
GAAGA	510520	1.9950219	23.056715	6
GAGCA	354670	1.9851294	32.13942	9
CTCCA	357425	1.9216931	30.512817	24
TCTCG	325555	1.7847489	30.490337	41
CCAGT	308820	1.6937298	30.693424	26
ATCGG	299080	1.6732687	31.43869	2
GATCG	291760	1.6323154	31.145061	1
TCTGA	426055	1.6307559	22.211624	18
GCACA	296495	1.6268296	31.323448	11
AGCAC	296130	1.624827	31.26677	10
TCGGA	288130	1.6120067	31.864271	3
AGAGC	287685	1.6102065	31.799076	8
ATGCC	288150	1.5803647	30.115099	47
GTCTG	281380	1.5735682	31.606916	17
TGAAC	394935	1.5122895	22.075216	20
GTCAC	274665	1.5064061	30.35533	29
CGTCT	268360	1.4711958	30.887577	16
TGAAT	543395	1.4521434	15.304957	37
AAGAG	369975	1.4457967	22.516327	7
CACGT	256545	1.4070264	30.957226	14
CTCGT	254790	1.3968028	29.93394	42
GAACT	362940	1.389774	21.936745	21
CACTT	370095	1.388663	21.018858	33
ACGTC	252515	1.3849239	30.921415	15
TCACA	368195	1.3821259	21.019173	30
CTTGA	359250	1.375055	21.282894	35
ACTCC	253810	1.3646078	30.055489	23
CAGTC	247110	1.3552802	30.31504	27
ACACG	242880	1.3326511	30.91896	13
ATCTC	350240	1.3141634	20.97548	40
AACTC	346165	1.2994299	21.40717	22
GAATC	334545	1.2810435	21.114752	38
ACTTG	325750	1.2468313	21.2621	34
AGTCA	310950	1.1906933	21.417953	28
AATCT	437930	1.1472523	14.774854	39
ACACT	292635	1.0984896	20.737953	32
TATGC	261100	0.99937886	20.848316	46
GTATG	247125	0.96489394	21.198875	45
TCGTA	251150	0.96129453	20.889746	43
CGTAT	238145	0.91151696	20.832184	44
>>END_MODULE