##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW236_CAGATC_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	7827595	
Filtered Sequences	0	
Sequence length	51	
%GC	37	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74262401158977	34.0	31.0	34.0	30.0	34.0
2	32.071198624864984	34.0	31.0	34.0	30.0	34.0
3	32.486776078731715	34.0	31.0	34.0	30.0	34.0
4	36.11538218316098	37.0	35.0	37.0	35.0	37.0
5	36.089855313158125	37.0	35.0	37.0	35.0	37.0
6	36.10763267644788	37.0	36.0	37.0	35.0	37.0
7	36.106683598218865	37.0	36.0	37.0	35.0	37.0
8	36.100942626694405	37.0	36.0	37.0	35.0	37.0
9	37.640559584393415	39.0	38.0	39.0	35.0	39.0
10	37.754086152898815	39.0	38.0	39.0	35.0	39.0
11	37.79580075361589	39.0	38.0	39.0	35.0	39.0
12	37.78064028606488	39.0	38.0	39.0	35.0	39.0
13	37.76752987347966	39.0	38.0	39.0	35.0	39.0
14	39.23161149752893	40.0	39.0	41.0	36.0	41.0
15	39.1865842573613	40.0	39.0	41.0	36.0	41.0
16	39.18050320692371	40.0	39.0	41.0	36.0	41.0
17	39.152953621131395	40.0	39.0	41.0	36.0	41.0
18	39.132006318671316	40.0	39.0	41.0	36.0	41.0
19	39.16618169437739	40.0	39.0	41.0	36.0	41.0
20	39.13248897011151	40.0	39.0	41.0	36.0	41.0
21	39.08652312747402	40.0	39.0	41.0	36.0	41.0
22	39.035138123523254	40.0	39.0	41.0	36.0	41.0
23	38.96714584747934	40.0	38.0	41.0	36.0	41.0
24	38.8833276376716	40.0	38.0	41.0	35.0	41.0
25	38.863470069670186	40.0	38.0	41.0	35.0	41.0
26	38.75859775576023	40.0	38.0	41.0	35.0	41.0
27	38.66116616917457	40.0	38.0	41.0	35.0	41.0
28	38.56287505932537	40.0	38.0	41.0	34.0	41.0
29	38.27316921736497	40.0	38.0	41.0	34.0	41.0
30	38.25347057429517	40.0	38.0	41.0	34.0	41.0
31	38.50798361437964	40.0	38.0	41.0	34.0	41.0
32	38.48512103653804	40.0	38.0	41.0	34.0	41.0
33	38.509162903803784	40.0	38.0	41.0	34.0	41.0
34	38.634978176566364	40.0	38.0	41.0	35.0	41.0
35	38.692964825083564	40.0	38.0	41.0	35.0	41.0
36	38.70050928797415	40.0	38.0	41.0	35.0	41.0
37	38.66302638294393	40.0	38.0	41.0	35.0	41.0
38	38.61776382145474	40.0	38.0	41.0	35.0	41.0
39	38.54041924754666	40.0	38.0	41.0	34.0	41.0
40	38.50820181677769	40.0	38.0	41.0	34.0	41.0
41	38.50266422828468	40.0	38.0	41.0	34.0	41.0
42	38.45601324544768	40.0	38.0	41.0	34.0	41.0
43	38.41254932070451	40.0	38.0	41.0	34.0	41.0
44	38.34517856378619	40.0	38.0	41.0	34.0	41.0
45	38.22561987430367	40.0	38.0	41.0	33.0	41.0
46	38.16594381799263	40.0	38.0	41.0	33.0	41.0
47	38.10013548222666	40.0	38.0	41.0	33.0	41.0
48	38.059657276596454	40.0	38.0	41.0	33.0	41.0
49	38.008213761698194	40.0	37.0	41.0	33.0	41.0
50	37.92390497975432	40.0	37.0	41.0	33.0	41.0
51	36.93083073408882	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	7.0
14	10.0
15	29.0
16	57.0
17	132.0
18	328.0
19	678.0
20	1366.0
21	2073.0
22	3457.0
23	5988.0
24	9513.0
25	14565.0
26	21481.0
27	30855.0
28	43258.0
29	58188.0
30	77488.0
31	100377.0
32	130148.0
33	169859.0
34	227092.0
35	317229.0
36	470267.0
37	782093.0
38	1650328.0
39	3709516.0
40	1207.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.649197873539183	28.696855095744933	33.084469964610115	20.569477066105772
2	18.482776946671965	31.3690931691619	31.192793695121996	18.955336189044136
3	18.727067509241344	30.649988917413328	31.448752777832784	19.17419079551254
4	18.814233490618765	29.70185606179165	31.12801569319823	20.355894754391354
5	20.114402955186108	30.617514064026054	31.292178504380974	17.975904476406864
6	20.024260325170122	31.105812193911415	30.086469726652947	18.783457754265516
7	19.016262338559926	32.21159244953271	30.794809900103417	17.977335311803945
8	18.31271290862647	32.417377240391204	30.90134326060559	18.368566590376737
9	19.496337824627457	30.77254449263298	31.07482151589992	18.656296166839645
10	18.320058715352545	32.07280141601603	30.881643723263657	18.725496145367764
11	19.560835735625055	30.7261809023078	30.892068381156662	18.820914980910484
12	18.35663444519038	30.76303768909863	30.78276277707265	20.097565088638337
13	18.302773712743186	32.11622471525418	30.868868407218308	18.71213316478433
14	18.331262667524317	30.989927302064046	30.85356357859598	19.82524645181566
15	18.279944222970144	32.18785591232045	30.895428289276587	18.63677157543281
16	18.297535833164595	30.920276278984797	30.96468327755843	19.81750461029218
17	19.581904733434055	30.843832728166536	30.954185922264237	18.620076616135176
18	18.384318555060656	30.740808639179722	32.12999650595106	18.74487629980856
19	18.39793355599125	30.723880119368086	30.910272980585376	19.967913344055287
20	18.45812155585464	30.730217902178126	32.04940981233699	18.762250729630235
21	19.706356409454486	30.670362954741044	30.812634017910618	18.810646617893855
22	18.483318232896266	31.910494011693004	30.807140079421657	18.79904767598908
23	18.51377873033467	31.897164737917823	30.782931243431687	18.806125288315823
24	18.528809206680783	30.66947747678686	30.745931512056142	20.055781804476215
25	18.47765501408798	30.720930247413158	31.987053494719643	18.81436124377922
26	18.52132131794379	30.778669939934982	30.743279686019815	19.956729056101413
27	18.554764529533205	30.734066071135086	30.688863164142145	20.022306235189564
28	18.552277682610427	31.944375737323284	30.711674113240328	18.791672466825965
29	19.78756140337118	30.741034403887646	30.682265108279633	18.789139084461546
30	18.613179648405332	30.688294333021677	31.82424919702233	18.87427682155066
31	18.574901224857758	30.699316763742534	30.657908118547546	20.06787389285216
32	18.637666463565505	31.924126691648326	30.597520205155615	18.840686639630555
33	18.646874155334707	30.748672976908807	30.59551978345897	20.008933084297517
34	18.623627715276747	30.78814165067973	30.63381352490908	19.954417109134443
35	18.625043216862665	31.95141159752778	30.65453519609404	18.76900998951552
36	19.78071122548462	30.781502157493858	30.624440182412688	18.81334643460883
37	18.594901744826018	32.013960448463244	30.660204887690572	18.73093291902017
38	18.606163920079467	30.83027142886901	31.83695226138327	18.726612389668244
39	18.619276081529797	30.820466915976464	30.648109634973906	19.91214736751983
40	18.615011939442077	31.97256707908914	30.659579892663235	18.75284108880555
41	18.643589371383122	30.78826540261105	31.814267036178716	18.753878189827105
42	18.64715145796713	30.730460725689348	30.612096253584927	20.010291562758596
43	18.599725573189897	30.728084637337737	31.86523849550319	18.806951293969178
44	18.652362154671955	30.752750134639843	30.680226693251782	19.91466101743642
45	19.797677429905438	30.790566882646903	30.674687512177236	18.737068175270423
46	18.612937240737413	30.82585618077412	31.84495728153826	18.71624929695021
47	18.677634334466507	31.993351219807632	30.6602858684161	18.668728577309764
48	18.678828936457297	30.776961025453627	31.827419793800203	18.716790244288877
49	19.822146455163452	30.79356382630064	30.675845770308346	18.708443948227565
50	18.720668674988218	30.772719094967304	30.639817717730306	19.866794512314172
51	18.695835336740412	30.72657649237163	30.602775198495426	19.974812972392527
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.218674939644956
2	37.4381131357161
3	37.90125830475389
4	39.17012824501012
5	38.090307431592976
6	38.80771807943564
7	36.993597650363874
8	36.6812794990032
9	38.152633991467106
10	37.04555486072031
11	38.38175071653554
12	38.45419953382872
13	37.014906877527515
14	38.15650911933998
15	36.91671579840296
16	38.11504044345677
17	38.20198134956923
18	37.12919485486922
19	38.36584690004654
20	37.22037228548488
21	38.51700302734834
22	37.28236590888534
23	37.31990401865049
24	38.584591011157
25	37.2920162578672
26	38.4780503740452
27	38.57707076472276
28	37.34395014943639
29	38.576700487832724
30	37.487456469955994
31	38.642775117709924
32	37.47835310319606
33	38.655807239632225
34	38.57804482441119
35	37.39405320637818
36	38.594057660093455
37	37.32583466384619
38	37.332776309747715
39	38.531423449049626
40	37.36785302824763
41	37.39746756121023
42	38.65744302072573
43	37.40667686715907
44	38.56702317210837
45	38.53474560517586
46	37.32918653768762
47	37.34636291177627
48	37.39561918074617
49	38.53059040339102
50	38.58746318730239
51	38.67064830913294
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	70.0
1	75.5
2	81.0
3	142.5
4	204.0
5	399.0
6	594.0
7	1218.0
8	1842.0
9	3436.0
10	5030.0
11	8527.0
12	12024.0
13	18694.5
14	25365.0
15	36379.5
16	47394.0
17	64568.0
18	81742.0
19	105822.0
20	129902.0
21	160329.5
22	190757.0
23	225488.5
24	260220.0
25	294449.0
26	359530.0
27	390382.0
28	417436.5
29	444491.0
30	466766.0
31	489041.0
32	503165.0
33	517289.0
34	530685.5
35	544082.0
36	551281.5
37	558481.0
38	558303.0
39	558125.0
40	552026.5
41	545928.0
42	531716.0
43	517504.0
44	494726.0
45	471948.0
46	436297.0
47	400646.0
48	365440.0
49	330234.0
50	299697.0
51	269160.0
52	274278.0
53	279396.0
54	210555.5
55	141715.0
56	119632.5
57	97550.0
58	82652.0
59	67754.0
60	56844.5
61	45935.0
62	37354.5
63	28774.0
64	23260.5
65	17747.0
66	14456.5
67	11166.0
68	8909.5
69	6653.0
70	5365.5
71	4078.0
72	3244.0
73	2410.0
74	1937.0
75	1138.5
76	813.0
77	627.5
78	442.0
79	359.5
80	277.0
81	189.5
82	102.0
83	70.5
84	39.0
85	35.5
86	32.0
87	27.0
88	22.0
89	13.5
90	5.0
91	3.5
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04970875473245613
2	6.387658022674909E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	8.942721231744872E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.2775316045349817E-5
18	0.0
19	5.365632739046923E-4
20	0.0
21	0.003615414440833998
22	8.942721231744872E-5
23	0.003078851166929306
24	0.008559461750384377
25	0.0
26	0.043525501766506824
27	0.036754584262471426
28	0.05792328294961607
29	0.6352398150389743
30	0.06179420371135707
31	0.009287654764969316
32	0.09185452236606519
33	0.035132119124711994
34	0.01805152157207929
35	0.010079724359781005
36	0.017693812722809496
37	0.037278372220330765
38	0.03413564447317471
39	0.025422878930246133
40	0.03436560016199101
41	0.022727287244677322
42	0.02257398345213313
43	0.023366053046944817
44	0.014819366612605788
45	0.00625990486222141
46	0.035234321653074795
47	0.01529205330628373
48	0.01539425583464653
49	0.016199100745503566
50	0.019047996223616578
51	0.017170024764950154
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7827595.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	12.01508435977177
#Duplication Level	Relative count
1	100.0
2	5.994923518803019
3	0.9880212855965979
4	0.5883597176763966
5	0.4503150535479705
6	0.38184935319395275
7	0.37238661412063323
8	0.3618106116269232
9	0.3111571259991539
10++	1.8775187584886335
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC	79796	1.0194191191547342	TruSeq Adapter, Index 7 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	3732395	3.5301256	3.7293928	8
AAAAA	3740550	3.5296223	3.6846182	14
TTTTC	1964510	3.009996	3.3194056	1
CTCCA	516130	2.0743742	19.277742	24
CACCA	515445	2.070658	19.102032	31
GAGCA	489185	2.046052	20.387703	9
GGAAG	475395	2.0288823	20.931301	5
CGGAA	483065	2.0204546	20.650858	4
GAAGA	781625	2.0171156	13.395442	6
CTGAA	791200	2.00199	13.053639	19
TCCAG	479250	1.9653906	19.581068	25
CCAGA	451605	1.8511579	19.076925	33
TCACC	419980	1.6879385	18.657377	30
TCTCG	411235	1.6872475	19.146738	41
TCGGA	401765	1.681193	20.24686	3
TCTGA	627665	1.5889328	12.664655	18
GCACA	377295	1.5465564	19.506186	11
AGAGC	365770	1.5298595	19.855473	8
CACAC	376465	1.5123439	19.064856	12
TCATC	595290	1.4768876	11.840654	38
AGCAC	355950	1.4590621	19.427273	10
ATCGG	340140	1.4233221	19.679028	2
CCAGT	346895	1.4226062	18.939634	26
TGAAC	554170	1.402228	12.484772	20
GTCAC	331850	1.3609073	18.81278	29
AAGAG	524255	1.352929	12.669598	7
CTCGT	328925	1.3495395	18.678255	42
ACCAG	326900	1.339984	18.636148	32
CACGT	321680	1.3192003	19.224487	14
GTCTG	312690	1.3090656	19.549387	17
CGTCT	317835	1.3040384	19.11601	16
ACTCC	321310	1.2913744	18.655773	23
ATGCC	301785	1.2376117	18.467907	47
ACACG	301450	1.235663	19.149149	13
AACTC	497145	1.2328207	11.988774	22
GATCG	291210	1.2185736	19.203474	1
GAACT	477330	1.2077981	12.190076	21
CATCT	486500	1.2069844	11.611704	39
ACGTC	294125	1.2061981	19.112902	15
CAGTC	291830	1.1967864	18.753603	27
GATCA	467445	1.182786	11.799403	36
ATCTC	471730	1.1703408	11.721028	40
CAGAT	457625	1.1579381	11.772887	34
AGTCA	423620	1.0718946	11.902296	28
AGATC	415650	1.0517279	11.608994	35
ATCAT	619640	0.9485216	7.356214	37
TCGTA	328095	0.8305719	11.487225	43
TATGC	314955	0.79730797	11.40843	46
GTATG	301870	0.77975166	11.606732	45
CGTAT	288935	0.7314384	11.3671055	44
>>END_MODULE