##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW235_GCCAAT_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	6441440	
Filtered Sequences	0	
Sequence length	51	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.676610043716934	34.0	31.0	34.0	30.0	34.0
2	32.01712427655928	34.0	31.0	34.0	30.0	34.0
3	32.45121354852331	34.0	31.0	34.0	30.0	34.0
4	36.09576895849375	37.0	35.0	37.0	35.0	37.0
5	36.0730608373283	37.0	35.0	37.0	35.0	37.0
6	36.09171582751683	37.0	35.0	37.0	35.0	37.0
7	36.09252527385181	37.0	35.0	37.0	35.0	37.0
8	36.08698955513053	37.0	35.0	37.0	35.0	37.0
9	37.63033017461934	39.0	38.0	39.0	35.0	39.0
10	37.7408823182394	39.0	38.0	39.0	35.0	39.0
11	37.77735785787029	39.0	38.0	39.0	35.0	39.0
12	37.7611482836136	39.0	38.0	39.0	35.0	39.0
13	37.75064535259197	39.0	38.0	39.0	35.0	39.0
14	39.20501673538836	40.0	39.0	41.0	36.0	41.0
15	39.16498593482203	40.0	39.0	41.0	36.0	41.0
16	39.14989319158449	40.0	39.0	41.0	36.0	41.0
17	39.126014369457764	40.0	39.0	41.0	36.0	41.0
18	39.09938678307956	40.0	38.0	41.0	36.0	41.0
19	39.134879623189846	40.0	39.0	41.0	36.0	41.0
20	39.10128232196527	40.0	39.0	41.0	36.0	41.0
21	39.052697222981195	40.0	39.0	41.0	36.0	41.0
22	39.012138900618496	40.0	39.0	41.0	36.0	41.0
23	38.94308384460618	40.0	38.0	41.0	35.0	41.0
24	38.84444006309148	40.0	38.0	41.0	35.0	41.0
25	38.82246128194938	40.0	38.0	41.0	35.0	41.0
26	38.70757749819916	40.0	38.0	41.0	35.0	41.0
27	38.60727135547331	40.0	38.0	41.0	34.0	41.0
28	38.53104367967411	40.0	38.0	41.0	34.0	41.0
29	38.2275778397377	40.0	38.0	41.0	34.0	41.0
30	38.22307962194789	40.0	38.0	41.0	34.0	41.0
31	38.467133280757096	40.0	38.0	41.0	34.0	41.0
32	38.44400429096599	40.0	38.0	41.0	34.0	41.0
33	38.46456273752453	40.0	38.0	41.0	34.0	41.0
34	38.59836294368961	40.0	38.0	41.0	35.0	41.0
35	38.658580379542464	40.0	38.0	41.0	35.0	41.0
36	38.6683221453588	40.0	38.0	41.0	35.0	41.0
37	38.621558378250825	40.0	38.0	41.0	35.0	41.0
38	38.573409206637024	40.0	38.0	41.0	34.0	41.0
39	38.507218416999926	40.0	38.0	41.0	34.0	41.0
40	38.46828550758836	40.0	38.0	41.0	34.0	41.0
41	38.46427537941827	40.0	38.0	41.0	34.0	41.0
42	38.41332543654835	40.0	38.0	41.0	34.0	41.0
43	38.37030881293624	40.0	38.0	41.0	34.0	41.0
44	38.30040099729253	40.0	38.0	41.0	34.0	41.0
45	38.191566171539286	40.0	38.0	41.0	33.0	41.0
46	38.142546852877615	40.0	38.0	41.0	33.0	41.0
47	38.07035569686281	40.0	38.0	41.0	33.0	41.0
48	38.02447806701607	40.0	38.0	41.0	33.0	41.0
49	37.97624304503341	40.0	37.0	41.0	33.0	41.0
50	37.88883075833975	40.0	37.0	41.0	33.0	41.0
51	36.86152009488561	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	7.0
15	17.0
16	43.0
17	112.0
18	226.0
19	584.0
20	1150.0
21	1733.0
22	3075.0
23	5083.0
24	8020.0
25	12339.0
26	18386.0
27	26028.0
28	36453.0
29	49006.0
30	65454.0
31	84859.0
32	109791.0
33	142450.0
34	190465.0
35	266228.0
36	395043.0
37	652626.0
38	1365668.0
39	3005582.0
40	1005.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.853239935387673	29.114376242544733	32.31723720178926	21.71514662027833
2	18.9939789823263	31.22763309299355	30.601778858006195	19.17660906667395
3	18.715132020169403	30.65319245386125	30.679537494721675	19.95213803124767
4	18.750341538537967	29.18114583074592	30.944664547057798	21.123848083658313
5	20.036994833453388	30.191975707295267	31.107718149978886	18.66331130927246
6	19.876580391962044	30.739632752924813	29.628530266524255	19.75525658858889
7	19.336514816562755	31.560132516952734	30.43158051615788	18.671772150326635
8	18.55471757867806	31.777925432822475	30.580662087980325	19.086694900519138
9	19.623788915423685	30.196321100400425	30.809212292226096	19.37067769194979
10	18.790223925085073	31.32296505129288	30.397581907151196	19.489229116470852
11	19.765626940559873	30.2879325119849	30.336306788544178	19.61013375891105
12	18.803606025981768	30.312725104945475	30.238487046374722	20.64518182269803
13	18.712663628008645	31.49457264214213	30.29280719839042	19.499956531458803
14	18.781328398618943	30.599043071114533	30.273308452768326	20.3463200774982
15	18.71741411858218	31.534920763059194	30.275186914727144	19.472478203631486
16	18.78738294542835	30.471493951662982	30.349937281104843	20.39118582180382
17	19.822989890459276	30.393747360838567	30.36945155120594	19.413811197496212
18	18.877409399140564	30.264102436722222	31.27931021634914	19.579177947788075
19	18.895921532464264	30.30069596779577	30.27303113147938	20.530351368260575
20	18.996482153058945	30.268495864278794	31.17670893464815	19.55831304801411
21	19.998298455817604	30.23477894208664	30.142886241214377	19.624036360881377
22	19.0294272983314	31.19851883912806	30.194192783780753	19.577861078759792
23	19.076856916858592	31.21053273003475	30.175144440019842	19.537465913086812
24	19.08648857703166	30.1829156108389	30.125252315730915	20.60534349639853
25	19.037777267194915	30.275776844929087	31.08536290022107	19.601082987654934
26	19.08903971298554	30.292248435243142	30.142357929395686	20.476353922375633
27	19.122501836054504	30.252261231769328	30.048179250479766	20.577057681696402
28	19.120024605137253	31.258823054268	30.013265648494485	19.60788669210026
29	20.12843345862665	30.308124693406324	30.000968601682864	19.562473246284164
30	19.218988257312617	30.202374635099318	30.892081981331497	19.686555126256568
31	19.15475747796648	30.211054449361914	30.006761629653667	20.62742644301794
32	19.233266165121922	31.178118411631328	29.94241082083615	19.646204602410595
33	19.271667740298334	30.26141119306051	29.901584108843267	20.56533695779789
34	20.14569555138838	30.325891160926417	29.88983862345094	19.63857466423427
35	19.262927194470503	30.292143742347616	29.94687618094765	20.49805288223423
36	19.23833098561807	30.27742168692658	29.91383636548834	20.570410961967013
37	19.20154358797234	31.283172729283198	29.932331359535237	19.58295232320923
38	19.26065186649344	31.290045150912505	29.935315890220267	19.513987092373792
39	19.281068384655494	30.34011420890596	30.79494355240196	19.583873854036582
40	19.252896857972505	31.212516799400174	29.932796187363124	19.601790155264194
41	19.27968904598907	30.279584699005536	30.84433169173168	19.596394563273712
42	19.29666913558393	30.243071892075708	29.9265225377985	20.53373643454186
43	19.25647420829207	30.241766302719064	30.848806308383715	19.652953180605152
44	19.317833703721472	30.24969486258996	29.931957579339997	20.500513854348572
45	20.22739714202587	30.23783640782442	29.935991961579028	19.59877448857068
46	19.253334194725856	30.30485530313925	30.83717413971269	19.604636362422205
47	19.37365421270628	31.233879277096012	29.896119350332928	19.49634715986478
48	19.367057711453292	30.19468602244165	30.827910896691012	19.61034536941404
49	20.270100313302454	30.25963205516657	29.881613347696785	19.588654283834195
50	19.44360510921727	30.24633082837056	29.839966635001385	20.47009742741079
51	19.36951981418433	30.208648241923086	29.82679076530027	20.595041178592318
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.56838655566601
2	38.17058804900025
3	38.66727005141708
4	39.874189622196276
5	38.70030614272585
6	39.631836980550936
7	38.00828696688939
8	37.6414124791972
9	38.99446660737348
10	38.279453041555925
11	39.37576069947092
12	39.4487878486798
13	38.21262015946745
14	39.127648476117145
15	38.189892322213666
16	39.17856876723217
17	39.236801087955484
18	38.456587346928636
19	39.42627290072485
20	38.55479520107305
21	39.62233481669898
22	38.60728837709119
23	38.61432282994541
24	39.691832073430184
25	38.63886025484985
26	39.565393635361175
27	39.69955951775091
28	38.72791129723752
29	39.69090670491081
30	38.90554338356919
31	39.78218392098442
32	38.87947076753252
33	39.83700469809622
34	39.78427021562265
35	39.76098007670473
36	39.808741947585084
37	38.78449591118157
38	38.77463895886723
39	38.86494223869208
40	38.8546870132367
41	38.876083609262786
42	39.83040557012579
43	38.90942738889722
44	39.818347558070045
45	39.826171630596555
46	38.857970557148064
47	38.87000137257106
48	38.97740308086733
49	39.858754597136645
50	39.91370253662805
51	39.96456099277665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	7.0
2	10.0
3	21.5
4	33.0
5	96.0
6	159.0
7	411.5
8	664.0
9	1384.5
10	2105.0
11	3897.0
12	5689.0
13	9427.5
14	13166.0
15	19963.5
16	26761.0
17	37803.0
18	48845.0
19	64893.0
20	80941.0
21	102583.5
22	124226.0
23	150199.0
24	176172.0
25	202292.0
26	255661.5
27	282911.0
28	308713.0
29	334515.0
30	356729.5
31	378944.0
32	395120.0
33	411296.0
34	426335.5
35	441375.0
36	451028.0
37	460681.0
38	465151.5
39	469622.0
40	468932.0
41	468242.0
42	461839.5
43	455437.0
44	439720.0
45	424003.0
46	395385.0
47	366767.0
48	337802.0
49	308837.0
50	281959.0
51	255081.0
52	249812.0
53	244543.0
54	191845.5
55	139148.0
56	118863.0
57	98578.0
58	84366.5
59	70155.0
60	59328.0
61	48501.0
62	39609.0
63	30717.0
64	24438.5
65	18160.0
66	14821.5
67	11483.0
68	9094.0
69	6705.0
70	5201.5
71	3698.0
72	2920.0
73	2142.0
74	1688.5
75	944.5
76	654.0
77	521.5
78	389.0
79	307.5
80	226.0
81	164.5
82	103.0
83	75.0
84	47.0
85	40.5
86	34.0
87	21.0
88	8.0
89	7.5
90	7.0
91	5.0
92	3.0
93	2.5
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047194416155393826
2	6.209791599393924E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.657343699545443E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	7.762239499242405E-4
20	0.0
21	0.003601679127648476
22	9.314687399090887E-5
23	0.00287202861471969
24	0.009330211878089372
25	0.0
26	0.04253707245584838
27	0.036653294915422636
28	0.05837204103430289
29	0.6281204202786954
30	0.06321567848183016
31	0.010634268113962096
32	0.09091134901512705
33	0.034712735040612036
34	0.018023920117240865
35	0.00990461760103331
36	0.017635808142278744
37	0.0375071412603393
38	0.033315531930748404
39	0.025801684095481756
40	0.03449539233463325
41	0.021547976849896917
42	0.02126853622792419
43	0.023317767455724187
44	0.013956506619637845
45	0.005806155145433319
46	0.034992175662584765
47	0.015089793586527237
48	0.014220422762612086
49	0.01580391962045754
50	0.01797734668024541
51	0.016750912839365112
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6441440.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	12.520302134904023
#Duplication Level	Relative count
1	100.0
2	6.1436424541016335
3	1.030091880616713
4	0.5374149279438986
5	0.445702078638176
6	0.39760875522176053
7	0.3377717132966855
8	0.3651737231502245
9	0.31819884911558616
10++	2.2693337956257444
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC	52881	0.8209499739188753	TruSeq Adapter, Index 6 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2564245	3.1877859	3.4192364	13
TTTTT	2474570	3.1852577	3.5305502	1
GAAGA	636555	1.9964632	11.102642	6
CTCCA	437110	1.9827775	14.741218	24
GAGCA	409280	1.9716179	15.828231	9
GGAAG	385200	1.9189315	16.39636	5
CTGAA	607970	1.8567761	10.561799	19
TCCAG	391850	1.838121	15.09952	25
CGGAA	373800	1.8007007	15.870077	4
GCCAA	367375	1.7113577	14.466535	34
CGCCA	231695	1.6577717	21.285835	33
TCTCG	334155	1.5784296	14.771915	41
TCGGA	320740	1.5558882	15.754291	3
TCTGA	483520	1.4870133	10.293684	18
CACAC	322520	1.4528369	14.424474	12
AGCAC	308850	1.4387285	14.923201	10
GCACA	307465	1.4322766	14.902421	11
AGAGC	292495	1.4090313	15.361129	8
TGAAC	449060	1.3714556	10.151749	20
CCAAT	450830	1.3314326	9.39691	35
ATCGG	271750	1.3182409	15.229272	2
CCAGT	280345	1.3150645	14.501037	26
AAGAG	419155	1.3146193	10.369976	7
GTCAC	277110	1.2998894	14.394661	29
CTCGT	271275	1.2814068	14.449513	42
CACGT	269880	1.2659745	14.715769	14
CACGC	174535	1.2487934	21.025694	31
CGTCT	258285	1.2200466	14.726603	16
ACGCC	167910	1.2013917	20.895567	32
GTCTG	245225	1.1978793	15.184763	17
ACTCC	263055	1.1932455	14.002327	23
AACTC	400490	1.1827639	9.565911	22
ATGCC	249260	1.1692485	14.261129	47
ACACG	249870	1.1639795	14.516354	13
ATCTC	386410	1.1491529	9.505202	40
TCACG	244365	1.1462867	14.295002	30
GAACT	375200	1.1458828	9.848403	21
GATCG	235575	1.1427584	14.808164	1
ACGTC	242315	1.1366704	14.578194	15
CAGTC	236300	1.1084547	14.298216	27
AGTCA	347220	1.0604303	9.583257	28
CAATA	470955	0.9055427	6.1696177	36
TCGTA	279610	0.8599102	9.404096	43
ATATC	419940	0.81309223	6.1843534	38
TATGC	252355	0.7760905	9.2747755	46
GTATG	240820	0.7658857	9.596387	45
CGTAT	240340	0.7391396	9.273057	44
TATCT	333935	0.65108484	6.08623	39
>>END_MODULE