##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW234_ACAGTG_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8776965	
Filtered Sequences	0	
Sequence length	51	
%GC	37	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.754685702859703	34.0	31.0	34.0	30.0	34.0
2	32.081854604638394	34.0	31.0	34.0	30.0	34.0
3	32.4999433175363	34.0	31.0	34.0	30.0	34.0
4	36.125487341011386	37.0	35.0	37.0	35.0	37.0
5	36.10141182060086	37.0	35.0	37.0	35.0	37.0
6	36.12034991594475	37.0	36.0	37.0	35.0	37.0
7	36.1121605247372	37.0	36.0	37.0	35.0	37.0
8	36.10784707470065	37.0	36.0	37.0	35.0	37.0
9	37.65299645150687	39.0	38.0	39.0	35.0	39.0
10	37.76056746267075	39.0	38.0	39.0	35.0	39.0
11	37.806216727536224	39.0	38.0	39.0	35.0	39.0
12	37.787773108358074	39.0	38.0	39.0	35.0	39.0
13	37.778492109744086	39.0	38.0	39.0	35.0	39.0
14	39.24121925973272	40.0	39.0	41.0	36.0	41.0
15	39.205288844150566	40.0	39.0	41.0	36.0	41.0
16	39.197328005751416	40.0	39.0	41.0	36.0	41.0
17	39.17194918744691	40.0	39.0	41.0	36.0	41.0
18	39.144415979783446	40.0	39.0	41.0	36.0	41.0
19	39.18620559612577	40.0	39.0	41.0	36.0	41.0
20	39.15283813937962	40.0	39.0	41.0	36.0	41.0
21	39.10737173954778	40.0	39.0	41.0	36.0	41.0
22	39.04398718691484	40.0	39.0	41.0	36.0	41.0
23	38.983660866825836	40.0	39.0	41.0	36.0	41.0
24	38.90445216541253	40.0	38.0	41.0	35.0	41.0
25	38.88411700399853	40.0	38.0	41.0	35.0	41.0
26	38.78315237670425	40.0	38.0	41.0	35.0	41.0
27	38.682270465929854	40.0	38.0	41.0	35.0	41.0
28	38.58646023995766	40.0	38.0	41.0	34.0	41.0
29	38.297118764857785	40.0	38.0	41.0	34.0	41.0
30	38.28645380265274	40.0	38.0	41.0	34.0	41.0
31	38.53483054791719	40.0	38.0	41.0	34.0	41.0
32	38.515816686064035	40.0	38.0	41.0	34.0	41.0
33	38.53985962117885	40.0	38.0	41.0	34.0	41.0
34	38.66779826511784	40.0	38.0	41.0	35.0	41.0
35	38.733208916749696	40.0	38.0	41.0	35.0	41.0
36	38.735566565435775	40.0	38.0	41.0	35.0	41.0
37	38.69578721118291	40.0	38.0	41.0	35.0	41.0
38	38.61015180076484	40.0	38.0	41.0	35.0	41.0
39	38.559236820472684	40.0	38.0	41.0	34.0	41.0
40	38.510327203082156	40.0	38.0	41.0	34.0	41.0
41	38.5183418186127	40.0	38.0	41.0	34.0	41.0
42	38.48446530207196	40.0	38.0	41.0	34.0	41.0
43	38.448042461146876	40.0	38.0	41.0	34.0	41.0
44	38.38684989629103	40.0	38.0	41.0	34.0	41.0
45	38.28420085986443	40.0	38.0	41.0	34.0	41.0
46	38.22441721027713	40.0	38.0	41.0	33.0	41.0
47	38.15737444549454	40.0	38.0	41.0	33.0	41.0
48	38.121274381292395	40.0	38.0	41.0	33.0	41.0
49	38.075210736285264	40.0	38.0	41.0	33.0	41.0
50	37.98912858829903	40.0	37.0	41.0	33.0	41.0
51	36.9852197200285	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	4.0
14	11.0
15	29.0
16	62.0
17	163.0
18	313.0
19	761.0
20	1383.0
21	2153.0
22	3700.0
23	6500.0
24	10279.0
25	16048.0
26	23535.0
27	33879.0
28	47293.0
29	63994.0
30	85896.0
31	111753.0
32	143666.0
33	187712.0
34	251698.0
35	351489.0
36	522587.0
37	865278.0
38	1831985.0
39	4213191.0
40	1596.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.18932289060325	29.25803466537113	33.02918813579531	19.523454308230306
2	18.385306529823538	31.991612129940204	30.926687600262504	18.69639373997375
3	18.699561864494164	30.415297315188113	31.960694841553998	18.92444597876373
4	18.75316809398237	29.548494268804763	31.259860327573367	20.438477309639495
5	20.196810628731	30.416858219213587	31.336458559422304	18.049872592633104
6	20.172645099986156	30.85465192125068	30.087564437137438	18.88513854162572
7	18.92933377312089	32.185841005404484	30.76162432002406	18.12320090145056
8	18.392257460295216	32.54598827726897	30.65397890956612	18.4077753528697
9	19.687197040707588	30.809529446575084	30.836600313798744	18.66667319891858
10	18.244370348975984	32.3271426968206	30.75700996870786	18.671476985495556
11	19.667493262192572	30.78048049639027	30.815173582212076	18.736852659205088
12	18.204083074274536	30.747564790334703	30.84748543488552	20.200866700505244
13	18.160184072740407	32.363134637086965	30.922579730009176	18.55410156016345
14	18.16549342511905	30.980333178951952	30.947246570995784	19.90692682493322
15	18.115760972044438	32.38309597907705	30.952145758812982	18.54899729006553
16	18.155706442944684	30.88772713574681	31.020722994793758	19.935843426514747
17	19.636514444343803	30.812951857504274	31.023833409384675	18.526700288767245
18	18.244381742436026	30.682849937307488	32.42191349743334	18.650854822823153
19	18.2688929065095	30.734455887118127	30.973401518441833	20.023249687930534
20	18.32101415466508	30.65704375031688	32.35221970236864	18.669722392649398
21	19.77939641649067	30.64413657697107	30.876867797364092	18.699599209174163
22	18.32380477544076	32.11318648427831	30.901706276292597	18.661302463988342
23	18.420339020128964	32.04543937098953	30.84093280554231	18.693288803339204
24	18.40148802663389	30.65951078754361	30.78968121349283	20.149319972329668
25	18.375577434796654	30.715834004123295	32.21456391816533	18.69402464291472
26	18.41977812324716	30.691115685876465	30.807628963910634	20.081477226965745
27	18.4540747853689	30.69530504258429	30.719194417454286	20.13142575459252
28	18.472173851086566	32.12318916993232	30.712510153188454	18.69212682579266
29	19.906033499644437	30.719049199177288	30.710438202280688	18.664479098897583
30	18.523632707178795	30.614510809402695	32.048388365541705	18.813468117876813
31	18.47922470815458	30.661425128617086	30.724266611972357	20.135083551255974
32	18.50403183042022	32.1008966845593	30.659119600135476	18.735951884884997
33	18.51298781524692	30.711964874065355	30.654727034086033	20.120320276601692
34	18.550615373167307	32.10932552757952	30.643433404285407	18.696625694967768
35	18.52976245007459	30.748811657854088	30.685229826670003	20.03619606540132
36	18.514483657845656	32.09636786949113	30.6527939040312	18.73635456863201
37	19.855185515633757	30.78254940784444	30.70892033334139	18.65334474318042
38	18.533505538857252	30.785392133040162	32.04030265743881	18.640799670663775
39	19.908617579053043	30.768169159475693	30.66535168488119	18.657861576590072
40	18.48325810709806	32.13723581253566	30.689454657853027	18.690051422513253
41	18.537310037859665	30.743023562974887	32.05056206024407	18.66910433892138
42	18.521423623175707	30.672392107534236	30.701041343902176	20.105142925387884
43	18.514742195623267	30.712079433405187	32.06795071606122	18.70522765491032
44	18.562918603393737	30.704432516891135	30.718448398594354	20.014200481120778
45	19.92885510151878	30.741717470208645	30.691309873975314	18.638117554297263
46	18.542510222973544	30.778416264848357	32.04863169781713	18.630441814360967
47	18.591466961725363	32.1734275105391	30.66502728286379	18.570078244871752
48	18.594533911110076	30.71243938380812	32.035238638295624	18.65778806678618
49	19.929559055825155	30.728118421373495	30.698126094942126	18.644196427859224
50	18.626786018708696	30.708582097376024	30.6217596859579	20.042872197957376
51	18.63437768396357	30.66465379013688	30.60174000322033	20.09922852267922
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	37.71277719883356
2	37.08170026979729
3	37.62400784325789
4	39.19164540362187
5	38.2466832213641
6	39.05778364161188
7	37.05253467457145
8	36.80003281316492
9	38.35387023962616
10	36.91584733447154
11	38.404345921397656
12	38.40494977477978
13	36.71428563290386
14	38.07242025005227
15	36.66475826210996
16	38.09154986945943
17	38.16321473311105
18	36.895236565259175
19	38.29214259444004
20	36.99073654731448
21	38.47899562566483
22	36.9851072394291
23	37.113627823468164
24	38.55080799896356
25	37.06960207771137
26	38.5012553502129
27	38.58550053996142
28	37.16430067687923
29	38.570512598542024
30	37.33710082505561
31	38.614308259410556
32	37.23998371530522
33	38.63330809184861
34	37.247241068135075
35	38.565958515475906
36	37.25083822647767
37	38.508530258814176
38	37.17430520952102
39	38.566479155643115
40	37.173309529611316
41	37.20641437678104
42	38.62656654856359
43	37.21996985053359
44	38.57711908451451
45	38.566972655816045
46	37.17295203733451
47	37.16154520659711
48	37.25232197789626
49	38.57375548368438
50	38.66965821666607
51	38.73360620664279
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	112.0
1	106.5
2	101.0
3	156.5
4	212.0
5	460.5
6	709.0
7	1399.0
8	2089.0
9	3901.0
10	5713.0
11	9706.0
12	13699.0
13	21274.5
14	28850.0
15	41337.0
16	53824.0
17	73236.0
18	92648.0
19	119909.0
20	147170.0
21	181590.5
22	216011.0
23	255250.5
24	294490.0
25	332224.5
26	405299.0
27	440639.0
28	471666.0
29	502693.0
30	527439.5
31	552186.0
32	567854.0
33	583522.0
34	598798.0
35	614074.0
36	620950.5
37	627827.0
38	626856.5
39	625886.0
40	617394.5
41	608903.0
42	593118.5
43	577334.0
44	551403.0
45	525472.0
46	485836.5
47	446201.0
48	406079.5
49	365958.0
50	330456.5
51	294955.0
52	310118.0
53	325281.0
54	240076.0
55	154871.0
56	130688.0
57	106505.0
58	90001.0
59	73497.0
60	61339.5
61	49182.0
62	40072.0
63	30962.0
64	24887.5
65	18813.0
66	15034.0
67	11255.0
68	8982.5
69	6710.0
70	5230.0
71	3750.0
72	2972.0
73	2194.0
74	1704.5
75	938.0
76	661.0
77	516.0
78	371.0
79	315.0
80	259.0
81	169.5
82	80.0
83	71.5
84	63.0
85	44.5
86	26.0
87	20.0
88	14.0
89	10.0
90	6.0
91	7.5
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04888933703165046
2	5.6967300200012186E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.2786920080004876E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	8.31722582920178E-4
20	0.0
21	0.003611726832680773
22	1.82295360640039E-4
23	0.002950906150360631
24	0.008864111911121898
25	0.0
26	0.04233809750864906
27	0.036345137527607776
28	0.05799271160361241
29	0.6330092463624954
30	0.06192345531741326
31	0.01042501593660223
32	0.0910907130198195
33	0.03473865966196743
34	0.017751010742323797
35	0.00994649061492213
36	0.01813838838368388
37	0.03710849935028794
38	0.0336448874981272
39	0.024974464407685346
40	0.03403226513948728
41	0.0219551974970847
42	0.02121462259448454
43	0.02298060890068492
44	0.013740512808242941
45	0.0058106646204012435
46	0.03530833266396756
47	0.014925432652403195
48	0.014321579270283066
49	0.01580272907548338
50	0.018013060323243856
51	0.01668002549856357
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	8776965.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	12.09711749710086
#Duplication Level	Relative count
1	100.0
2	6.548361239757749
3	1.1221945137157108
4	0.6501603135019594
5	0.5115559315995725
6	0.38352778767367296
7	0.29223815461346636
8	0.2605094406840043
9	0.2031750979693623
10++	1.357098325614535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC	107683	1.2268819574875827	TruSeq Adapter, Index 5 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	4238815	3.540963	3.7842376	15
AAAAA	4190730	3.5135098	3.6845748	13
TTTTC	2220325	3.0215316	3.3787098	1
CTCCA	598720	2.1637974	22.523823	24
GAGCA	570450	2.1356006	23.767235	9
CGGAA	563300	2.1088328	24.1144	4
GGAAG	549220	2.091925	24.330437	5
TCCAG	560595	2.0612915	22.748001	25
CACAC	568075	2.0545347	22.465601	12
GAAGA	889925	2.0466194	15.424171	6
CTGAA	901235	2.03568	15.038784	19
TCGGA	474585	1.7754217	23.707838	3
TCTCG	475830	1.7483448	22.39843	41
TCTGA	720850	1.627052	14.664605	18
GCACA	441780	1.6255904	22.89999	11
CAGTG	434510	1.6255012	22.463291	35
AGAGC	430330	1.6110315	23.294369	8
CACAG	422945	1.5562845	22.134377	33
AGCAC	422140	1.5533223	22.889624	10
ATCGG	403795	1.5105964	23.108866	2
CCAGT	407460	1.4982184	22.127697	26
GTCAC	391275	1.4387066	22.133818	29
TGAAC	635000	1.434317	14.404114	20
CTCGT	387610	1.4241974	22.081072	42
TCACA	637475	1.4152633	13.880475	30
AAGAG	605110	1.3916116	14.748179	7
CACGT	376120	1.3829823	22.585564	14
GTCTG	368560	1.377783	22.915806	17
CGTCT	373345	1.3717835	22.540043	16
ACTCC	377640	1.3648057	21.8602	23
AGTGA	574705	1.320729	14.108338	36
ATGCC	356135	1.309498	22.008303	47
ACACG	355140	1.3067865	22.536293	13
GATCG	343040	1.283312	22.688673	1
ACACA	577280	1.2825536	13.738673	32
AACTC	577040	1.2810911	13.932197	22
ACGTC	344355	1.2661833	22.475245	15
CAGTC	343910	1.264547	21.934828	27
GAACT	553840	1.2509955	14.186657	21
ACAGT	544745	1.2304521	13.822197	34
TGATC	544835	1.2297632	13.784545	38
ATCTC	546520	1.212454	13.618326	40
GTGAT	499020	1.145966	13.961749	37
AGTCA	490880	1.1087835	13.803366	28
GATCT	478495	1.0800252	13.725413	39
TCGTA	388290	0.8764209	13.591022	43
TATGC	371280	0.83802706	13.506125	46
GTATG	359100	0.8246492	13.727067	45
CGTAT	344470	0.77751344	13.465949	44
>>END_MODULE