##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW233_TGACCA_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9726786	
Filtered Sequences	0	
Sequence length	51	
%GC	37	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.748588177019624	34.0	31.0	34.0	30.0	34.0
2	32.07652322154512	34.0	31.0	34.0	30.0	34.0
3	32.493935406824	34.0	31.0	34.0	30.0	34.0
4	36.12031168363322	37.0	35.0	37.0	35.0	37.0
5	36.099553336528636	37.0	35.0	37.0	35.0	37.0
6	36.12066524338049	37.0	36.0	37.0	35.0	37.0
7	36.12110639629576	37.0	36.0	37.0	35.0	37.0
8	36.11323411453691	37.0	36.0	37.0	35.0	37.0
9	37.6588843426801	39.0	38.0	39.0	35.0	39.0
10	37.773628000040304	39.0	38.0	39.0	35.0	39.0
11	37.81255987332301	39.0	38.0	39.0	35.0	39.0
12	37.79534442312188	39.0	38.0	39.0	35.0	39.0
13	37.782197017596566	39.0	38.0	39.0	35.0	39.0
14	39.24485056009251	40.0	39.0	41.0	36.0	41.0
15	39.20751201887242	40.0	39.0	41.0	36.0	41.0
16	39.1910583824914	40.0	39.0	41.0	36.0	41.0
17	39.17032738254959	40.0	39.0	41.0	36.0	41.0
18	39.15561882414191	40.0	39.0	41.0	36.0	41.0
19	39.184800714233866	40.0	39.0	41.0	36.0	41.0
20	39.149464478811396	40.0	39.0	41.0	36.0	41.0
21	39.10380139955788	40.0	39.0	41.0	36.0	41.0
22	39.05779617234305	40.0	39.0	41.0	36.0	41.0
23	38.992995630828105	40.0	39.0	41.0	36.0	41.0
24	38.89787284309534	40.0	38.0	41.0	35.0	41.0
25	38.88196008424571	40.0	38.0	41.0	35.0	41.0
26	38.77216945042278	40.0	38.0	41.0	35.0	41.0
27	38.670742730435315	40.0	38.0	41.0	35.0	41.0
28	38.60043954909669	40.0	38.0	41.0	34.0	41.0
29	38.29697384110229	40.0	38.0	41.0	34.0	41.0
30	38.29672936157946	40.0	38.0	41.0	34.0	41.0
31	38.535889758446416	40.0	38.0	41.0	34.0	41.0
32	38.518226472752666	40.0	38.0	41.0	34.0	41.0
33	38.53229216721741	40.0	38.0	41.0	34.0	41.0
34	38.66811514101369	40.0	38.0	41.0	35.0	41.0
35	38.72270665767706	40.0	38.0	41.0	35.0	41.0
36	38.72924571384628	40.0	38.0	41.0	35.0	41.0
37	38.690089305963966	40.0	38.0	41.0	35.0	41.0
38	38.6442562836275	40.0	38.0	41.0	35.0	41.0
39	38.57149730651008	40.0	38.0	41.0	34.0	41.0
40	38.52787436672298	40.0	38.0	41.0	34.0	41.0
41	38.5232648276625	40.0	38.0	41.0	34.0	41.0
42	38.476442064213195	40.0	38.0	41.0	34.0	41.0
43	38.4344497761131	40.0	38.0	41.0	34.0	41.0
44	38.36608186918063	40.0	38.0	41.0	34.0	41.0
45	38.2614828783115	40.0	38.0	41.0	34.0	41.0
46	38.21741868280026	40.0	38.0	41.0	33.0	41.0
47	38.150592189444694	40.0	38.0	41.0	33.0	41.0
48	38.100038080410116	40.0	38.0	41.0	33.0	41.0
49	38.0528507566631	40.0	38.0	41.0	33.0	41.0
50	37.9640533882415	40.0	37.0	41.0	33.0	41.0
51	36.96548500193178	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	5.0
14	13.0
15	24.0
16	74.0
17	183.0
18	396.0
19	845.0
20	1599.0
21	2408.0
22	4237.0
23	7265.0
24	11583.0
25	17953.0
26	26485.0
27	37532.0
28	52190.0
29	71116.0
30	94039.0
31	122469.0
32	158843.0
33	207485.0
34	278612.0
35	388631.0
36	580345.0
37	963702.0
38	2043014.0
39	4654062.0
40	1668.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.167482311690712	29.46905345610421	32.225154644774236	20.138309587430843
2	18.91435670528785	31.12964549646718	31.21141968169136	18.74457811655361
3	18.927320905384367	30.968009371235265	31.076935382355487	19.02773434102488
4	18.97429428384669	30.336546933385804	31.29367706866379	19.395481714103713
5	19.16047088935646	31.200778962341722	31.36846024987082	18.270289898430992
6	19.161858809271635	31.45084100750238	30.568627704978805	18.818672478247183
7	18.857359460771523	31.75673855680592	31.04082890278454	18.345073079638023
8	18.359661660079702	31.916811987022232	31.078960717342806	18.64456563555526
9	18.897666371296655	31.136851721684344	31.16730372210422	18.798178184914786
10	18.422950808211468	31.61447162505683	31.156869288581042	18.805708278150664
11	18.864689734101276	31.06374500271724	31.181080780434566	18.89048448274692
12	18.41370828966526	31.011672303677702	31.157630074312316	19.416989332344723
13	18.417933734740334	31.520010823719165	31.212828163383055	18.84922727815745
14	18.449927859007076	31.158791814685756	31.203266937300768	19.1880133890064
15	18.411210033817955	31.59235743440845	31.20087149033607	18.79556104143753
16	18.430209115323397	31.098268225496067	31.24079217945167	19.230730479728862
17	18.944037629695977	31.0760203833003	31.19336644190589	18.786575545097836
18	18.537767768304967	30.948054167121597	31.594423892948814	18.919754171624625
19	18.55987307548295	30.96839573768193	31.104083737940186	19.36764744889493
20	18.635600700992086	30.96525409318145	31.5109122376086	18.88823296821787
21	19.077723021012087	30.90367306711833	31.05723373453148	18.961370177338104
22	18.602498629041857	31.370616814989226	31.085054510364895	18.94183004560402
23	18.614058703825958	31.367399144213117	31.099923333059508	18.918618818901418
24	18.63144607373651	30.88208485774223	31.0888112062831	19.397657862238162
25	18.620857907226497	30.95434607073703	31.511806674887268	18.912989347149203
26	18.64256982758718	30.96436827821375	31.08168053771513	19.31138135648394
27	18.647919973794945	30.980185255748683	31.02741194746853	19.34448282298784
28	18.680899639008334	31.432279936061896	30.99468310593351	18.892137318996262
29	19.16896959513638	31.014885315714164	30.950179147314653	18.8659659418348
30	18.752848927599615	30.946583102581553	31.343753571607497	18.956814398211332
31	18.73220125327031	30.96681954254451	30.91449455594964	19.386484648235538
32	18.74257185505077	31.3870624584667	30.93581197817764	18.93455370830489
33	18.769208523473118	30.964787750315008	30.89962635038157	19.366377375830304
34	18.745380533026335	31.028926243590327	31.338165753364372	18.887527470018966
35	19.125776440303703	31.04625061023283	30.970668107782647	18.857304841680826
36	18.679497634835656	31.456989154989085	30.971493710564097	18.892019499611163
37	18.6928592725089	31.065642331840998	30.973769786716442	19.267728608933655
38	18.712397817655493	31.092207870875104	30.95400903629915	19.241385275170252
39	18.75302654847964	31.487601087619023	30.933220187938314	18.826152175963028
40	18.738855609840982	31.43773714449794	30.98783750957217	18.83556973608891
41	18.740227114506393	30.993685093798106	31.404375085479007	18.861712706216498
42	18.734919211447707	30.96995107410434	31.00929416176755	19.285835552680407
43	18.721598861105974	30.97444789574233	31.405327686029196	18.8986255571225
44	18.746164065951795	30.99880962014472	30.993678761133015	19.261347552770474
45	19.19433482099528	31.00963950655465	30.998915920505222	18.797109751944845
46	18.70360903014983	31.0849284170231	31.41633270670525	18.79512984612182
47	18.752369452730065	31.50747217554294	30.991860753143307	18.74829761858369
48	18.753958715837822	31.049884343803264	31.39716140537908	18.798995534979827
49	19.168430977982013	31.05402134448234	30.96105696214897	18.81649071538668
50	18.77636182790353	31.03002889768935	30.96989521541339	19.223714058993732
51	18.787248744493663	30.993320418378723	30.93841129606094	19.281019541066676
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.30579189912156
2	37.65893482184146
3	37.955055246409245
4	38.3697759979504
5	37.430760787787456
6	37.980531287518815
7	37.202432540409546
8	37.00422729563496
9	37.69584455621144
10	37.228659086362136
11	37.7551742168482
12	37.830697622009986
13	37.26716101289779
14	37.637941248013476
15	37.20677107525548
16	37.66093959505226
17	37.73061317479381
18	37.45752193992959
19	37.92752052437788
20	37.52383366920996
21	38.03909319835019
22	37.544328674645875
23	37.532677522727376
24	38.029103935974675
25	37.5338472543757
26	37.95395118407112
27	37.992402796782784
28	37.57303695800459
29	38.03493553697118
30	37.70966332581095
31	38.11868590150584
32	37.67712556335566
33	38.13558589930342
34	37.6329080030453
35	37.983081281984525
36	37.571517134446815
37	37.96058788144256
38	37.953783092825745
39	37.57917872444266
40	37.57442534592989
41	37.60193982072288
42	38.020754764128114
43	37.620224418228474
44	38.00751161872227
45	37.991444572940125
46	37.49873887627165
47	37.50066707131376
48	37.552954250817656
49	37.98492169336869
50	38.000075886897264
51	38.06826828556034
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	123.0
1	111.0
2	99.0
3	192.5
4	286.0
5	550.5
6	815.0
7	1621.5
8	2428.0
9	4515.5
10	6603.0
11	11199.0
12	15795.0
13	24386.5
14	32978.0
15	46999.5
16	61021.0
17	82837.5
18	104654.0
19	135215.0
20	165776.0
21	203554.5
22	241333.0
23	284342.5
24	327352.0
25	370405.5
26	451148.0
27	488837.0
28	523330.5
29	557824.0
30	584155.5
31	610487.0
32	629255.5
33	648024.0
34	664906.0
35	681788.0
36	690865.5
37	699943.0
38	699609.5
39	699276.0
40	691097.5
41	682919.0
42	666842.5
43	650766.0
44	620916.0
45	591066.0
46	547214.0
47	503362.0
48	457672.0
49	411982.0
50	369838.5
51	327695.0
52	303358.0
53	279021.0
54	226508.5
55	173996.0
56	147109.0
57	120222.0
58	101933.0
59	83644.0
60	69707.5
61	55771.0
62	45693.5
63	35616.0
64	28649.5
65	21683.0
66	17454.5
67	13226.0
68	10307.0
69	7388.0
70	5782.0
71	4176.0
72	3303.5
73	2431.0
74	1903.0
75	1024.5
76	674.0
77	533.5
78	393.0
79	328.5
80	264.0
81	178.0
82	92.0
83	70.0
84	48.0
85	42.5
86	37.0
87	25.5
88	14.0
89	13.0
90	12.0
91	10.0
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04963612852179538
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	3.0842664781562996E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	6.168532956312599E-4
20	0.0
21	0.0034646593437955764
22	1.2337065912625198E-4
23	0.0030739855898957785
24	0.009098586110561083
25	0.0
26	0.04129832814251285
27	0.035684963152268386
28	0.05663741342721018
29	0.6328503577646306
30	0.061921789993117975
31	0.01059959579659715
32	0.08923811010132227
33	0.033289516187566995
34	0.016840094970733396
35	0.010178079377915789
36	0.017158802506809546
37	0.036322378224420686
38	0.03202496693152291
39	0.024931154031763422
40	0.03376243704755096
41	0.022371212854893692
42	0.021373966693623157
43	0.02327593102181954
44	0.013560491615627196
45	0.005777859202412802
46	0.03408114458362711
47	0.014794198206889717
48	0.014496052447334607
49	0.015997062133370675
50	0.01837194732155102
51	0.01671672431160714
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	9726786.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	11.221578970786725
#Duplication Level	Relative count
1	100.0
2	6.570030015648232
3	1.0419161009728635
4	0.6069954837308504
5	0.5117695867418821
6	0.399837392035551
7	0.33078469480378453
8	0.25170821894160034
9	0.20715809169529942
10++	1.4551185311823047
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC	35194	0.36182558144077603	TruSeq Adapter, Index 4 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	4721790	3.5242462	3.7702875	13
AAAAA	4686330	3.521779	3.7788656	5
TTTTC	2474735	3.0274403	3.2046943	14
GAAAA	2411755	3.009679	3.2509239	4
CTCCA	574040	1.8891118	8.152238	24
GAAGA	902695	1.8706211	5.946212	6
CTGAA	911460	1.8591911	5.8151097	19
GAGCA	543660	1.8440197	8.492828	9
CGGAA	532180	1.8050809	8.551756	4
GGAAG	517335	1.7802227	8.568552	5
TCCAG	522820	1.745549	8.123186	25
TCTCG	436375	1.4549423	7.65006	41
TCGGA	429260	1.4540008	8.10746	3
TCTGA	707460	1.441101	5.3870726	18
GCACA	401235	1.341444	7.8578653	11
CACAC	401960	1.3246229	7.7275405	12
CACTG	395830	1.3215652	7.5631275	31
AGAGC	384815	1.3052393	7.977605	8
TGAAC	614400	1.2532499	5.2039127	20
AGCAC	374135	1.2508411	7.829608	10
ATCGG	356520	1.207614	7.7575545	2
AAGAG	581345	1.2046995	5.2393026	7
CCAGT	356260	1.1894519	7.4960194	26
GTCAC	340715	1.1375517	7.393484	29
CTCGT	337495	1.125261	7.210599	42
CACGT	325705	1.0874374	7.5469117	14
ACTCC	327470	1.0776731	7.3807974	23
CGTCT	322280	1.0745318	7.532723	16
GTCTG	315840	1.0683595	7.667782	17
CTGAC	319790	1.0676888	7.176464	33
GAACT	522000	1.064773	5.0175977	21
GACCA	317320	1.0608922	7.1332383	35
ACACG	301090	1.0066307	7.496801	13
ATGCC	301035	1.0050712	7.1051917	47
GATCG	290920	0.98541176	7.4242883	1
ACGTC	290260	0.9690965	7.467781	15
CAGTC	288960	0.9647562	7.279075	27
TGACC	280730	0.9372785	6.9457836	34
>>END_MODULE