##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW231_CGATGT_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	14552538	
Filtered Sequences	0	
Sequence length	51	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74159043597756	34.0	31.0	34.0	30.0	34.0
2	32.06206711159249	34.0	31.0	34.0	30.0	34.0
3	32.474027279640154	34.0	31.0	34.0	30.0	34.0
4	36.105751793948244	37.0	35.0	37.0	35.0	37.0
5	36.08104146506953	37.0	35.0	37.0	35.0	37.0
6	36.09905942179983	37.0	36.0	37.0	35.0	37.0
7	36.08847302099469	37.0	35.0	37.0	35.0	37.0
8	36.08738846790848	37.0	35.0	37.0	35.0	37.0
9	37.62469659931484	39.0	38.0	39.0	35.0	39.0
10	37.729463273004335	39.0	38.0	39.0	35.0	39.0
11	37.776763613329855	39.0	38.0	39.0	35.0	39.0
12	37.75394237073973	39.0	38.0	39.0	35.0	39.0
13	37.74765391438937	39.0	38.0	39.0	35.0	39.0
14	39.21139480962015	40.0	39.0	41.0	36.0	41.0
15	39.16207736409965	40.0	39.0	41.0	36.0	41.0
16	39.16582220915692	40.0	39.0	41.0	36.0	41.0
17	39.13754982120645	40.0	39.0	41.0	36.0	41.0
18	39.10487600169812	40.0	38.0	41.0	36.0	41.0
19	39.14893999933208	40.0	39.0	41.0	36.0	41.0
20	39.11277249370522	40.0	39.0	41.0	36.0	41.0
21	39.06715103578496	40.0	39.0	41.0	36.0	41.0
22	39.0105853700571	40.0	39.0	41.0	36.0	41.0
23	38.9563977087708	40.0	38.0	41.0	36.0	41.0
24	38.86759512326991	40.0	38.0	41.0	35.0	41.0
25	38.8451492103989	40.0	38.0	41.0	35.0	41.0
26	38.74279661733232	40.0	38.0	41.0	35.0	41.0
27	38.63766375322298	40.0	38.0	41.0	34.0	41.0
28	38.530088084978715	40.0	38.0	41.0	34.0	41.0
29	38.24354803265245	40.0	38.0	41.0	34.0	41.0
30	38.21597435443907	40.0	38.0	41.0	34.0	41.0
31	38.475948593984086	40.0	38.0	41.0	34.0	41.0
32	38.461223464937866	40.0	38.0	41.0	34.0	41.0
33	38.4893505174149	40.0	38.0	41.0	34.0	41.0
34	38.62079769178407	40.0	38.0	41.0	35.0	41.0
35	38.6690331267302	40.0	38.0	41.0	35.0	41.0
36	38.63367750697507	40.0	38.0	41.0	35.0	41.0
37	38.622755013592815	40.0	38.0	41.0	35.0	41.0
38	38.57992530237681	40.0	38.0	41.0	34.0	41.0
39	38.493133637582666	40.0	38.0	41.0	34.0	41.0
40	38.46478188203322	40.0	38.0	41.0	34.0	41.0
41	38.46964701277537	40.0	38.0	41.0	34.0	41.0
42	38.423329868645595	40.0	38.0	41.0	34.0	41.0
43	38.38651230458907	40.0	38.0	41.0	34.0	41.0
44	38.32068323752187	40.0	38.0	41.0	34.0	41.0
45	38.18862338651856	40.0	38.0	41.0	33.0	41.0
46	38.10238832566525	40.0	38.0	41.0	33.0	41.0
47	38.0364607878021	40.0	38.0	41.0	33.0	41.0
48	38.01163453412731	40.0	38.0	41.0	33.0	41.0
49	37.95596163363394	40.0	37.0	41.0	33.0	41.0
50	37.88145923412122	40.0	37.0	41.0	33.0	41.0
51	36.882656001310565	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	9.0
12	11.0
13	15.0
14	23.0
15	63.0
16	119.0
17	240.0
18	535.0
19	1243.0
20	2443.0
21	3816.0
22	6780.0
23	11076.0
24	18180.0
25	27706.0
26	41263.0
27	58981.0
28	82264.0
29	110764.0
30	146419.0
31	190628.0
32	246458.0
33	321285.0
34	428983.0
35	596886.0
36	887789.0
37	1468750.0
38	3090366.0
39	6807097.0
40	2343.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.553027342595207	29.08262041431208	31.94323634664626	19.421115896446455
2	18.49601969581786	32.17190191864219	31.079219140642955	18.25285924489699
3	18.55536814265663	30.24082809472822	32.37998760078826	18.823816161826894
4	18.733962419476246	29.64248572998057	30.654907068444004	20.968644782099176
5	20.786862058013522	30.48941703502166	30.562339022925073	18.161381884039745
6	20.833369409514685	30.714930962557872	29.819581986317438	18.63211764161001
7	18.62718379433196	32.88184507747033	30.305126157375433	18.18584497082227
8	18.20496191111131	32.97973865452198	30.405699679327412	18.409599755039295
9	20.369472600875437	30.576105442030787	30.50859826612931	18.545823690964465
10	18.124075676696393	32.78667954689416	30.5579342929735	18.53131048343595
11	20.32211151072067	30.494495187025112	30.568832735568186	18.61456056668603
12	18.108016622255168	30.42457611174078	30.58265850259247	20.88474876341158
13	18.10998878683567	32.7213163779404	30.604998248415498	18.563696586808433
14	18.138952806720038	30.59616130189799	30.608035519302547	20.656850372079425
15	18.11804923649744	32.75833397583294	30.587104462465586	18.53651232520403
16	18.17247273293497	30.516752473005056	30.61665257290515	20.694122221154824
17	20.42634075238285	30.47329613569812	30.59726763812608	18.503095473792957
18	18.252754261833914	30.367871226311177	32.73809008435504	18.641284427499862
19	18.243952049311286	30.398826314469385	30.563582077244728	20.793639558974604
20	18.327222371795216	30.356065725442534	32.68820187928731	18.62851002347494
21	20.533755177126977	30.302295424886903	30.46570950089988	18.698239897086243
22	18.342183343290365	32.508809810750385	30.471727039003397	18.677279806955852
23	18.346519740854003	32.497777297663994	30.48720920532052	18.66849375616148
24	18.36779363772866	30.277028258931725	30.460016767017013	20.895161336322595
25	18.387927823169253	30.32515911143191	32.58464829878117	18.702264766617667
26	18.36901621099447	30.358307738515776	30.426483436715152	20.846192613774598
27	18.4139720362124	30.37149972321079	30.35303565208658	20.86149258849023
28	18.424558881932505	32.564030739375376	30.32241168012656	18.688998698565563
29	20.61332966900203	30.395586647661755	30.358775055723953	18.63230862761226
30	18.4773767897437	30.30736838804818	32.4608056675243	18.754449154683815
31	18.458192582020384	30.337745423397493	30.324234396929217	20.879827597652902
32	18.477616508502027	32.498425804765404	30.287495679775507	18.736462006957066
33	18.49980752574343	30.347684122248648	30.24797558326574	20.904532768742182
34	18.522148663485677	30.39751614693496	30.25499356528104	20.825341624298325
35	20.612588937003085	30.43524753917764	30.313345515747	18.63881800807228
36	18.46406250708768	32.50364847818219	30.32723519374907	18.705053820981064
37	18.448436103663983	30.47230356774593	32.460562315254	18.618698013336083
38	20.61779956867011	30.451334608139945	30.33024218621864	18.600623636971296
39	18.508136836822125	30.4392246384472	32.40650380575023	18.646134718980445
40	18.46923963307076	32.56085590884497	30.336986508345078	18.632917949739188
41	18.497757577844535	30.402668238709378	32.45025524730771	18.649318936138375
42	18.498077119888293	30.332513612883233	30.376172163701142	20.793237103527332
43	18.4891966765857	30.34962768230741	32.46180837979772	18.69936726130916
44	18.521707426616455	30.387208354939215	30.337168613859312	20.753915604585018
45	20.633363183325386	30.380442741514347	30.32957566120848	18.656618413951794
46	18.512245247697564	30.446487142611932	32.41850026365686	18.622767346033637
47	18.545070249846876	32.59334207172445	30.30542491343165	18.556162764997026
48	18.54936623430534	30.40735845853668	32.42687279257584	18.61640251458214
49	20.657116630648765	30.40472648708208	30.305600511114232	18.632556371154926
50	18.58346476115738	30.390277921823177	30.251245187995913	20.775012129023533
51	18.58176413883294	30.327942876284688	30.262321036500673	20.827971948381702
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	38.974143239041666
2	36.748878940714846
3	37.37918430448352
4	39.702607201575425
5	38.94824394205327
6	39.46548705112469
7	36.81302876515423
8	36.61456166615061
9	38.915296291839894
10	36.655386160132345
11	38.936672077406705
12	38.99276538566675
13	36.673685373644105
14	38.79580317879947
15	36.65456156170147
16	38.86659495408979
17	38.92943622617581
18	36.89403868933378
19	39.03759160828589
20	36.95573239527016
21	39.231995074213216
22	37.01946315024622
23	37.01501349701548
24	39.262954974051254
25	37.09019258978692
26	39.21520882476907
27	39.275464624702636
28	37.11355758049806
29	39.245638296614295
30	37.23182594442751
31	39.338020179673286
32	37.2140785154591
33	39.404340294485614
34	39.347490287784
35	39.25140694507537
36	37.16911632806874
37	37.067134117000066
38	39.218423205641415
39	37.15427155580257
40	37.102157582809944
41	37.14707651398291
42	39.291314223415625
43	37.188563937894855
44	39.27562303120148
45	39.289981597277176
46	37.135012593731204
47	37.101233014843906
48	37.16576874888749
49	39.289673001803685
50	39.35847689018091
51	39.40973608721464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	139.0
2	137.0
3	235.0
4	333.0
5	673.5
6	1014.0
7	2197.0
8	3380.0
9	6362.0
10	9344.0
11	15648.0
12	21952.0
13	34091.5
14	46231.0
15	66188.5
16	86146.0
17	117316.0
18	148486.0
19	192160.0
20	235834.0
21	291080.0
22	346326.0
23	408092.0
24	469858.0
25	531421.0
26	650329.0
27	707674.0
28	758924.0
29	810174.0
30	852440.5
31	894707.0
32	920844.5
33	946982.0
34	973829.0
35	1000676.0
36	1015254.5
37	1029833.0
38	1031411.0
39	1032989.0
40	1020113.0
41	1007237.0
42	985279.5
43	963322.0
44	920507.5
45	877693.0
46	812541.0
47	747389.0
48	679792.5
49	612196.0
50	557018.5
51	501841.0
52	577810.5
53	653780.0
54	461758.0
55	269736.0
56	226275.5
57	182815.0
58	155564.5
59	128314.0
60	107330.0
61	86346.0
62	70517.0
63	54688.0
64	44010.5
65	33333.0
66	26952.0
67	20571.0
68	16279.5
69	11988.0
70	9437.5
71	6887.0
72	5495.5
73	4104.0
74	3197.5
75	1785.5
76	1280.0
77	996.0
78	712.0
79	567.0
80	422.0
81	300.5
82	179.0
83	138.0
84	97.0
85	77.0
86	57.0
87	42.5
88	28.0
89	21.5
90	15.0
91	13.0
92	11.0
93	6.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049297242858943234
2	4.810157513417935E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	7.558818949656753E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	6.047055159725403E-4
20	0.0
21	0.0037656661676471826
22	8.245984308716458E-5
23	0.002968554351137925
24	0.009283604000896614
25	6.871653590597049E-6
26	0.043284545967170816
27	0.03688016482073436
28	0.05900001772886626
29	0.6350713531893887
30	0.062154106726950306
31	0.010032614242271691
32	0.09182590693114837
33	0.03530655614848764
34	0.017612048152700236
35	0.01036245361462035
36	0.01811367886481382
37	0.03805521758472646
38	0.034165861652448525
39	0.025864904115007293
40	0.03505230496563554
41	0.022779531652829216
42	0.022463435587661753
43	0.023391108822392356
44	0.014451087501025594
45	0.006095156734859582
46	0.0358769033965072
47	0.01502143474904515
48	0.015172611128038284
49	0.01625833239535262
50	0.018738999341558153
51	0.01720662059085501
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.4552538E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	16.036906213712037
#Duplication Level	Relative count
1	100.0
2	9.359303752054256
3	1.3019929171585307
4	0.6255352637548318
5	0.4652454690646483
6	0.36860866143554843
7	0.3066916649306761
8	0.29396106751845935
9	0.2592412563942319
10++	2.7521236951137653
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC	277259	1.905227802875347	TruSeq Adapter, Index 2 (100% over 51bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	6757355	3.513844	3.721446	13
AAAAA	6760340	3.4912324	3.6729507	11
CTCCA	1104750	2.36695	33.1168	24
GAGCA	1059720	2.353274	34.764187	9
GGAAG	1029140	2.3282712	35.677624	5
CGGAA	1042025	2.3139796	35.18489	4
TCCAG	1033375	2.255593	33.637653	25
CACCG	640890	2.243671	52.255756	31
GAAGA	1601325	2.2140577	22.532602	6
CTGAA	1596625	2.1698704	21.947351	19
TCGGA	900650	2.0027952	34.82468	3
TCACC	924780	1.9813606	32.645023	30
TCTCG	903095	1.9739463	33.182537	41
GCACA	841725	1.8347375	33.74178	11
AGAGC	825375	1.8328744	34.314262	8
CACAC	848870	1.8162147	33.07981	12
CGATG	810720	1.8028159	33.39662	34
AGCAC	812840	1.771776	33.691116	10
TCTGA	1296585	1.7645378	21.602837	18
ATCGG	781025	1.7367824	34.11936	2
CCAGT	780325	1.7032497	32.97595	26
CCGAT	773970	1.6893783	32.76027	33
GTCAC	765720	1.6713709	32.984776	29
CTCGT	751810	1.6432741	32.681053	42
CACGT	737950	1.6107559	33.44758	14
CGTCT	736065	1.6088593	33.431316	16
GTCTG	721555	1.6067528	34.08084	17
TGAAC	1167575	1.586776	21.354053	20
ACTCC	740425	1.5863761	32.540127	23
ACCGA	727190	1.5850815	32.67882	32
AAGAG	1118975	1.5471407	21.789253	7
GATCG	687240	1.5282307	33.613342	1
ACACG	699630	1.5250081	33.38356	13
ATGCC	695745	1.5186331	32.400402	47
ACGTC	685880	1.4971004	33.327835	15
CAGTC	682890	1.490574	32.704662	27
AACTC	1066560	1.4227823	20.733335	22
GAACT	1026940	1.3956482	21.11217	21
ATCTC	1014560	1.355283	20.448244	40
GATGT	940420	1.3038555	21.012121	35
AGTCA	929090	1.2626666	20.731802	28
TCGTA	744950	1.0138112	20.282614	43
TATGC	721945	0.9825035	20.190134	46
GTATG	700470	0.9711743	20.587772	45
GTATC	713295	0.97073156	20.304108	38
CGTAT	672930	0.9157985	20.149813	44
TGTAT	1019065	0.8646872	12.955917	37
ATGTA	1000660	0.84789985	12.881766	36
TATCT	919665	0.76596534	12.608032	39
>>END_MODULE