FastQCFastQC Report
Wed 23 Jul 2014
LW230_ATCACG_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW230_ATCACG_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12516168
Filtered Sequences 0
Sequence length 51
%GC 37

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC 16852 0.1346418488470273 TruSeq Adapter, Index 1 (100% over 51bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 6270600 3.6164432 3.8069541 6
TTTTT 6518815 3.5059657 4.599283 7
GAAAA 3144255 3.1059086 3.796855 2
TTTTC 3377810 3.027293 3.9332345 7
Produced by FastQC (version 0.10.1)