##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename LW230_ATCACG_L004_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12516168 Filtered Sequences 0 Sequence length 51 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77239351533153 34.0 31.0 34.0 30.0 34.0 2 32.10865202512462 34.0 31.0 34.0 30.0 34.0 3 32.50692815884223 34.0 31.0 34.0 30.0 34.0 4 36.137858008936924 37.0 35.0 37.0 35.0 37.0 5 36.1048830600548 37.0 35.0 37.0 35.0 37.0 6 36.117736674675506 37.0 36.0 37.0 35.0 37.0 7 36.12453124630478 37.0 36.0 37.0 35.0 37.0 8 36.11352444294452 37.0 36.0 37.0 35.0 37.0 9 37.6681608939733 39.0 38.0 39.0 35.0 39.0 10 37.79017787233281 39.0 38.0 39.0 35.0 39.0 11 37.82460973678206 39.0 38.0 39.0 35.0 39.0 12 37.804374869368964 39.0 38.0 39.0 35.0 39.0 13 37.78724286858406 39.0 38.0 39.0 35.0 39.0 14 39.248794439320406 40.0 39.0 41.0 36.0 41.0 15 39.211245886121056 40.0 39.0 41.0 36.0 41.0 16 39.19656447564462 40.0 39.0 41.0 36.0 41.0 17 39.17502849114841 40.0 39.0 41.0 36.0 41.0 18 39.16059484020988 40.0 39.0 41.0 36.0 41.0 19 39.18702105948083 40.0 39.0 41.0 36.0 41.0 20 39.148402130748 40.0 39.0 41.0 36.0 41.0 21 39.10528270314045 40.0 39.0 41.0 36.0 41.0 22 39.05728310773713 40.0 39.0 41.0 36.0 41.0 23 38.99089873194416 40.0 39.0 41.0 36.0 41.0 24 38.89935234170714 40.0 38.0 41.0 35.0 41.0 25 38.88351650441253 40.0 38.0 41.0 35.0 41.0 26 38.77307711114137 40.0 38.0 41.0 35.0 41.0 27 38.67312711047023 40.0 38.0 41.0 35.0 41.0 28 38.61109838091019 40.0 38.0 41.0 34.0 41.0 29 38.30292993830061 40.0 38.0 41.0 34.0 41.0 30 38.309530281153144 40.0 38.0 41.0 34.0 41.0 31 38.54728260279025 40.0 38.0 41.0 34.0 41.0 32 38.52749403811134 40.0 38.0 41.0 34.0 41.0 33 38.535418348491326 40.0 38.0 41.0 34.0 41.0 34 38.66930213784283 40.0 38.0 41.0 35.0 41.0 35 38.72397030784502 40.0 38.0 41.0 35.0 41.0 36 38.73208772844851 40.0 38.0 41.0 35.0 41.0 37 38.69391166689357 40.0 38.0 41.0 35.0 41.0 38 38.64660126006618 40.0 38.0 41.0 35.0 41.0 39 38.57446320631043 40.0 38.0 41.0 34.0 41.0 40 38.53119125598186 40.0 38.0 41.0 34.0 41.0 41 38.52101761497609 40.0 38.0 41.0 34.0 41.0 42 38.47131462281427 40.0 38.0 41.0 34.0 41.0 43 38.42713880158848 40.0 38.0 41.0 34.0 41.0 44 38.35767672661473 40.0 38.0 41.0 34.0 41.0 45 38.26127126129978 40.0 38.0 41.0 34.0 41.0 46 38.22757900021796 40.0 38.0 41.0 33.0 41.0 47 38.15618286683272 40.0 38.0 41.0 33.0 41.0 48 38.09926624506798 40.0 38.0 41.0 33.0 41.0 49 38.05795352059832 40.0 38.0 41.0 33.0 41.0 50 37.96655733607922 40.0 37.0 41.0 33.0 41.0 51 36.9693959045612 39.0 36.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 3.0 12 5.0 13 8.0 14 19.0 15 39.0 16 94.0 17 221.0 18 474.0 19 1069.0 20 2149.0 21 3255.0 22 5583.0 23 9380.0 24 15076.0 25 23189.0 26 34158.0 27 48816.0 28 67472.0 29 91962.0 30 121995.0 31 158398.0 32 205385.0 33 265895.0 34 357725.0 35 497392.0 36 742289.0 37 1230137.0 38 2594727.0 39 6036990.0 40 2262.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.808124776928548 28.80245051860491 31.982145490749204 27.407279213717338 2 19.807194920104916 29.82140534432115 33.63345191066528 16.73794782490865 3 17.349151912949715 33.1941134059562 29.612290279261195 19.84444440183289 4 17.345668418640596 30.466409527261057 32.072867670040864 20.115054384057483 5 18.60311398824305 31.485163829696116 32.41302769346017 17.498694488600663 6 17.949199787027467 32.17253875147728 31.008947786574932 18.86931367492031 7 18.663092409753528 32.041332458944304 32.18290134808034 17.11267378322183 8 16.2995654900126 31.315327502794787 34.33630804572134 18.048798961471274 9 17.268423238016315 29.48203854487993 34.833143352319716 18.41639486478404 10 17.910298104020335 30.206617552592775 32.898711490609585 18.984372852777305 11 18.32198960576432 30.22914042061436 32.241433640072586 19.207436333548735 12 18.402165902535025 30.747781589381027 31.410356588374334 19.43969591970961 13 18.33562796536448 31.37295696254637 31.179934625358175 19.11148044673098 14 18.429250869755023 31.455306448427343 31.14181592960401 18.97362675221362 15 18.189137442066933 31.711415187140346 31.183162450360207 18.916284920432517 16 18.182114525787764 31.36860259465996 31.36971315821264 19.07956972133963 17 18.49096304875422 31.257402425406884 31.432511931766978 18.819122594071924 18 18.31323293199644 31.113244884536545 31.569502742372908 19.004019441094112 19 18.308300707266962 31.092509419493314 31.30351022437881 19.29567964886091 20 18.417242401987576 31.169396256106502 31.46585280734487 18.94750853456106 21 18.637963477796617 31.145153430535288 31.2258918357852 18.990991255882896 22 18.329843707479924 31.309893636587493 31.333535099382832 19.026727556549755 23 18.374831542404813 31.385266944614877 31.401438554155835 18.83846295882448 24 18.40683081296886 31.15919985968899 31.39987798695251 19.034091340389633 25 18.22421207513354 31.227433188816256 31.639060773233467 18.909293962816733 26 18.29052751391602 31.200510600475113 31.614074604580377 18.894887281028492 27 18.30649688038773 31.224552219367375 31.474431312062652 18.994519588182246 28 18.267299595413895 31.36809804719593 31.465261387693 18.899340969697175 29 18.537902845282517 31.197618493934304 31.5318051450592 18.73267351572398 30 18.466641035720475 31.163425103473152 31.51909245664305 18.850841404163326 31 18.368306608335303 31.124439539727604 31.389378947732617 19.11787490420447 32 18.48607840435512 31.355016543063186 31.34574798132885 18.81315707125285 33 18.501919949410908 31.234288816274702 31.307060189958463 18.95673104435593 34 18.42356793640233 31.457104917668865 31.294885526250493 18.824441619678314 35 18.475664914857706 31.343010831991663 31.545681686102604 18.635642567048023 36 18.45489909652755 31.28094748736209 31.345403605898703 18.91874981021165 37 18.397725866747148 31.479898640385972 31.33109944041846 18.79127605244842 38 18.48181009570639 31.357962607316725 31.36499590430336 18.795231392673525 39 18.642944760369442 31.323358260227312 31.306655618948337 18.72704136045491 40 18.436504447765728 31.440101023825317 31.34607853323636 18.777315995172593 41 18.4497498940527 31.31155601916905 31.486553863462202 18.752140223316047 42 18.525945525077894 31.261558118019327 31.33312145364913 18.87937490325364 43 18.421530861955954 31.246804384284864 31.48446469251204 18.847200061247136 44 18.51572439217164 31.253128908763678 31.394478673158094 18.836668025906587 45 18.689625741018784 31.310402224592465 31.319151465825502 18.68082056856325 46 18.41992337936438 31.331418816501866 31.5000679759775 18.74858982815625 47 18.558890532968746 31.54262964469097 31.339868970289174 18.55861085205111 48 18.555130290641316 31.309452529112512 31.473632825063763 18.661784355182405 49 18.607116835187792 31.29178966735117 31.334373435272273 18.766720062188764 50 18.52953092698239 31.27023461242481 31.428459552351278 18.77177490824152 51 18.561312268015364 31.18464884490838 31.3279679949599 18.92607089211636 >>END_MODULE >>Per base GC content pass #Base %GC 1 39.21540399064589 2 36.54514274501357 3 37.19359631478261 4 37.46072280269808 5 36.10180847684371 6 36.818513461947774 7 35.775766192975354 8 34.34836445148387 9 35.68481810280035 10 36.89467095679764 11 37.52942593931306 12 37.84186182224464 13 37.44710841209546 14 37.40287762196864 15 37.105422362499446 16 37.2616842471274 17 37.31008564282614 18 37.31725237309055 19 37.60398035612788 20 37.36475093654863 21 37.628954733679514 22 37.356571264029675 23 37.213294501229285 24 37.4409221533585 25 37.133506037950276 26 37.18541479494451 27 37.301016468569976 28 37.16664056511107 29 37.2705763610065 30 37.3174824398838 31 37.48618151253977 32 37.299235475607965 33 37.45865099376684 34 37.24800955608064 35 37.11130748190573 36 37.373648906739206 37 37.18900191919557 38 37.277041488379915 39 37.36998612082435 40 37.21382044293832 41 37.20189011736875 42 37.405320428331535 43 37.268730923203094 44 37.35239241807823 45 37.370446309582036 46 37.16851320752063 47 37.11750138501986 48 37.21691464582372 49 37.373836897376556 50 37.30130583522391 51 37.48738316013172 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 204.0 1 201.5 2 199.0 3 308.0 4 417.0 5 833.5 6 1250.0 7 2442.5 8 3635.0 9 6942.5 10 10250.0 11 17200.5 12 24151.0 13 36932.0 14 49713.0 15 70058.0 16 90403.0 17 121866.0 18 153329.0 19 196894.0 20 240459.0 21 293355.5 22 346252.0 23 403613.0 24 460974.0 25 516705.5 26 622522.0 27 672607.0 28 712878.5 29 753150.0 30 784472.0 31 815794.0 32 834757.5 33 853721.0 34 869192.0 35 884663.0 36 890719.0 37 896775.0 38 889586.0 39 882397.0 40 865931.5 41 849466.0 42 822599.5 43 795733.0 44 754571.5 45 713410.0 46 657509.0 47 601608.0 48 545772.5 49 489937.0 50 438348.5 51 386760.0 52 346180.5 53 305601.0 54 256954.0 55 208307.0 56 177421.0 57 146535.0 58 125100.5 59 103666.0 60 87535.5 61 71405.0 62 59131.5 63 46858.0 64 38558.5 65 30259.0 66 25206.5 67 20154.0 68 16530.0 69 12906.0 70 10705.0 71 8504.0 72 6915.5 73 5327.0 74 4325.5 75 2572.5 76 1821.0 77 1391.5 78 962.0 79 712.5 80 463.0 81 332.5 82 202.0 83 150.5 84 99.0 85 72.5 86 46.0 87 30.0 88 14.0 89 12.5 90 11.0 91 7.0 92 3.0 93 3.5 94 4.0 95 2.0 96 0.0 97 0.5 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04924031061264119 2 3.1958663386429456E-5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 3.994832923303682E-5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 8.14945916353951E-4 20 0.0 21 0.003507463306660633 22 1.75772648625362E-4 23 0.0032517939995691976 24 0.008892498087273997 25 0.0 26 0.043208112898452625 27 0.03608932062912546 28 0.05860419898486501 29 0.6392052263919755 30 0.06272686656171442 31 0.009835278657173666 32 0.09212883687722952 33 0.034994736408140256 34 0.01798473782071318 35 0.010346617271356537 36 0.01780097550624121 37 0.03735967749873603 38 0.033996028177314334 39 0.0253831683946716 40 0.034099893833320225 41 0.022938330645609743 42 0.02218730205602865 43 0.02340972093055958 44 0.01454918150667201 45 0.006367763679746069 46 0.03511458139583937 47 0.015236292769480242 48 0.014996602794082022 49 0.0160592283516808 50 0.018679838749368016 51 0.017089895245893154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1.2516168E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Duplicate Percentage 13.131263046722761 #Duplication Level Relative count 1 100.0 2 8.14116760656636 3 1.1364414712664672 4 0.6244434804447591 5 0.49601589077376196 6 0.41096828694719056 7 0.3407612045937122 8 0.29110253658759333 9 0.26598780794082055 10++ 2.4509692002100505 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC 16852 0.1346418488470273 TruSeq Adapter, Index 1 (100% over 51bp) >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position AAAAA 6270600 3.6164432 3.8069541 6 TTTTT 6518815 3.5059657 4.599283 7 GAAAA 3144255 3.1059086 3.796855 2 TTTTC 3377810 3.027293 3.9332345 7 >>END_MODULE