FastQCFastQC Report
Wed 23 Jul 2014
LW228_GAGTGG_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW228_GAGTGG_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9431558
Filtered Sequences 0
Sequence length 51
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC 359815 3.8150112632504616 TruSeq Adapter, Index 7 (97% over 36bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 461200 3.0390239 3.4538465 47
CACAC 892570 2.6461737 58.748768 12
GGAAG 811865 2.4899714 60.64439 5
GCACA 797075 2.3899388 59.10931 11
TCCAG 775215 2.3418343 58.724724 25
GTCTG 757755 2.3324952 60.27331 17
CTCCA 779265 2.3275964 58.11251 24
GAGCA 759565 2.3033712 59.564423 9
GAAGA 1083165 2.2664077 41.920383 6
ATGCC 747130 2.2569926 57.253735 47
GTCAC 744000 2.2475376 58.601475 29
AGAGC 733650 2.2247846 59.466896 8
CCAGT 735665 2.2223582 58.53915 26
GTGGA 717490 2.2170367 58.018517 36
AGTGG 708930 2.190586 58.391346 35
CAGTC 722860 2.1836758 58.434235 27
GAGTG 697810 2.1562254 58.588646 34
AGCAC 710170 2.1293638 58.96429 10
AAGAG 983000 2.0568233 41.56079 7
CTGAA 973080 2.0282753 41.280537 19
ACGTC 671315 2.0279644 59.05383 15
GATCG 662535 2.0242043 58.877407 1
CACGT 668555 2.0196269 59.0599 14
ACACG 666705 1.999039 58.670746 13
TCTCG 652595 1.9862056 57.94613 41
ACTCC 663380 1.9814581 57.936935 23
TCACG 655445 1.980023 58.11322 30
CGGAA 650255 1.9718902 59.87058 4
GGATC 642675 1.9635268 57.624836 38
CGTCT 644680 1.9621159 59.323288 16
ATCGG 639100 1.9526047 59.30699 2
CACGA 640540 1.9205863 57.45097 31
CTCGT 617920 1.8806704 57.475765 42
ACGAG 618920 1.8768673 58.008766 32
TCTGA 890605 1.8702943 41.37595 18
TGAAC 887270 1.8494142 41.031944 20
TCGGA 604975 1.8483446 60.121822 3
CGAGT 599170 1.8306091 57.668747 33
TGGAT 839140 1.7822587 40.128838 37
ATCTC 848800 1.7624576 39.71787 40
GTATG 814435 1.7297876 40.37557 45
AGTCA 822560 1.7145333 40.58623 28
TATGC 797365 1.6744878 39.75883 46
GATCT 780380 1.6388187 39.973484 39
GAACT 778020 1.6216949 40.819035 21
AACTC 783865 1.6155044 40.30456 22
TCGTA 652235 1.3697108 39.579494 43
CGTAT 650755 1.3666029 39.545067 44
Produced by FastQC (version 0.10.1)