##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW228_GAGTGG_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	9431558	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.649744082578934	34.0	31.0	34.0	30.0	34.0
2	31.961011319656837	34.0	31.0	34.0	30.0	34.0
3	32.39566485197886	34.0	31.0	34.0	30.0	34.0
4	36.05443968006134	37.0	35.0	37.0	35.0	37.0
5	36.030562712968525	37.0	35.0	37.0	35.0	37.0
6	36.04454513241609	37.0	35.0	37.0	35.0	37.0
7	36.014903052072626	37.0	35.0	37.0	35.0	37.0
8	36.02779159074248	37.0	35.0	37.0	35.0	37.0
9	37.5457970994824	39.0	37.0	39.0	35.0	39.0
10	37.65857327071519	39.0	37.0	39.0	35.0	39.0
11	37.668995832926015	39.0	37.0	39.0	35.0	39.0
12	37.663396439909505	39.0	37.0	39.0	35.0	39.0
13	37.64806260004975	39.0	37.0	39.0	35.0	39.0
14	39.10514890540884	40.0	38.0	41.0	36.0	41.0
15	39.042985262880215	40.0	38.0	41.0	36.0	41.0
16	39.06240093100207	40.0	38.0	41.0	36.0	41.0
17	39.022044608112466	40.0	38.0	41.0	36.0	41.0
18	38.96589503028026	40.0	38.0	41.0	36.0	41.0
19	39.02869674342246	40.0	39.0	41.0	36.0	41.0
20	38.996293613419965	40.0	39.0	41.0	36.0	41.0
21	38.932674856052415	40.0	38.0	41.0	35.0	41.0
22	38.871295071291506	40.0	38.0	41.0	35.0	41.0
23	38.81649914043894	40.0	38.0	41.0	35.0	41.0
24	38.73964481796115	40.0	38.0	41.0	35.0	41.0
25	38.69869633415815	40.0	38.0	41.0	35.0	41.0
26	38.602055461038354	40.0	38.0	41.0	34.0	41.0
27	38.49186497077153	40.0	38.0	41.0	34.0	41.0
28	38.341869286071294	40.0	38.0	41.0	34.0	41.0
29	38.048715705294924	40.0	38.0	41.0	34.0	41.0
30	38.0229990633573	40.0	38.0	41.0	33.0	41.0
31	38.301476171805334	40.0	38.0	41.0	34.0	41.0
32	38.27622647286906	40.0	38.0	41.0	34.0	41.0
33	38.32679955952134	40.0	38.0	41.0	34.0	41.0
34	38.436111509890516	40.0	38.0	41.0	34.0	41.0
35	38.42629690661924	40.0	38.0	41.0	34.0	41.0
36	38.45333740194356	40.0	38.0	41.0	34.0	41.0
37	38.25045533304254	40.0	38.0	41.0	34.0	41.0
38	38.28167371711015	40.0	38.0	41.0	34.0	41.0
39	38.23949224507764	40.0	38.0	41.0	34.0	41.0
40	38.09623319922329	40.0	38.0	41.0	33.0	41.0
41	38.12939813337309	40.0	38.0	41.0	33.0	41.0
42	38.14382883506627	40.0	38.0	41.0	33.0	41.0
43	38.12395862910454	40.0	38.0	41.0	33.0	41.0
44	38.07956628162601	40.0	38.0	41.0	33.0	41.0
45	37.925396207074165	40.0	37.0	41.0	33.0	41.0
46	37.774638612199595	40.0	37.0	41.0	33.0	41.0
47	37.70334795163217	40.0	37.0	41.0	33.0	41.0
48	37.71090386127085	40.0	37.0	41.0	33.0	41.0
49	37.64928519763119	40.0	37.0	41.0	32.0	41.0
50	37.583390146145526	40.0	37.0	41.0	32.0	41.0
51	36.550691730889	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	3.0
13	9.0
14	34.0
15	67.0
16	217.0
17	403.0
18	798.0
19	1534.0
20	2689.0
21	4120.0
22	6737.0
23	10620.0
24	15748.0
25	23170.0
26	32907.0
27	45249.0
28	61447.0
29	81787.0
30	107219.0
31	137821.0
32	177461.0
33	229198.0
34	305475.0
35	419421.0
36	618804.0
37	999371.0
38	2008273.0
39	4139858.0
40	1114.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.966976961521915	25.763931577783307	31.271802275472034	20.997289185222744
2	19.354751151851417	32.529991077823325	28.630379613966095	19.48487815635916
3	19.21862750565707	28.201576028053903	33.091468026809565	19.488328439479456
4	19.00185526081693	27.23984732957164	29.557640423777283	24.200656985834154
5	23.616893412519968	28.090014396348938	29.616506625946638	18.67658556518446
6	23.740414892216112	28.79055613081105	27.9343773319318	19.53465164504104
7	19.672295923960814	32.89422595927417	28.9271189341146	18.506359182650417
8	19.119004516539047	33.3638620469704	28.535614158339477	18.981519278151076
9	23.285773494238544	28.631052115785337	28.934427263706304	19.148747126269818
10	19.109387865716354	32.98949123782094	28.486820523183976	19.41430037327873
11	23.404203207995963	28.502830603384933	28.538699544656353	19.554266643962748
12	19.044658369274725	28.638661820242213	28.42652295622844	23.89015685425462
13	19.00573584979279	33.047848510288546	28.466505745922362	19.479909893996307
14	19.060615435965087	28.802834059865823	28.385607128747974	23.750943375421112
15	18.982197851086745	33.117518865918015	28.506265878871762	19.394017404123474
16	19.06246030613394	28.726303755964818	28.534924982701693	23.676310955199554
17	23.398223284000373	28.63463279343667	28.64154575521881	19.325598167344143
18	19.056639422670145	28.69463348473285	32.84408578094944	19.404641311647556
19	19.00606329466365	28.668920954935512	28.637918483007912	23.68709726739293
20	19.067432973428144	28.71412125122912	32.866680139166824	19.351765636175912
21	23.376510736387402	28.765544739851418	28.53378318557094	19.324161338190237
22	19.02406233549222	33.094493001941885	28.55622276095804	19.32522190160785
23	19.013523294076034	33.00097484096012	28.603163387543706	19.382338477420145
24	19.07100204840722	28.68551594349628	28.58219427485133	23.661287733245167
25	19.0437889477009	28.66671657005131	32.93145204641693	19.358042435830853
26	19.072585790492138	28.702492227864408	28.586640762553394	23.638281219090057
27	19.08386979761345	28.79412870581959	28.512984123768526	23.609017372798437
28	19.135620614278203	33.01592319692431	28.521729628688597	19.326726560108888
29	23.44876360228484	28.775228575150958	28.542169257062337	19.233838565501866
30	19.19913946470741	28.69395903418302	32.774446540709015	19.332454960400543
31	19.092268295398515	28.746809604852263	28.542772767518677	23.618149332230544
32	19.139830909579455	33.06215645681835	28.448813790416	19.349198843186187
33	19.14450948959953	28.80344986096147	28.51076787576835	23.54127277367065
34	23.390912599071235	28.817140947726482	28.534435522876723	19.25751093032556
35	19.17647318303308	33.063461056807	28.537710190004194	19.222355570155727
36	23.42111868716567	28.799535348203054	28.591981829629017	19.187364135002262
37	19.156525563979233	28.829001879603748	32.79011749087908	19.224355065537935
38	23.46697472831067	28.768957585240194	28.577249879513168	19.18681780693597
39	23.439999380648075	28.85011269765837	28.520838063757402	19.18904985793615
40	19.16442760079084	33.015764798543465	28.597257798795578	19.222549801870116
41	19.116658142009303	28.78390592765753	32.83940871152464	19.260027218808528
42	19.112341145586438	28.775054493609765	28.638420131785775	23.474184229018025
43	19.114953043661963	28.742863542296128	32.837483368503484	19.304700045538432
44	19.15588189010111	28.785883921856726	28.599621983171637	23.45861220487053
45	23.35334700094528	28.792356740576743	28.654546071462843	19.199750187015134
46	19.132315629852123	28.843384133655753	32.817803061680465	19.206497174811663
47	19.116863285702923	33.07007767556534	28.664478672357575	19.14858036637417
48	19.167599130285033	28.890197212874018	32.80251480984105	19.1396888469999
49	23.36429510364869	28.77852931732393	28.59733339667644	19.25984218235094
50	19.17112297480427	28.7656135155751	28.55832585975808	23.504937649862548
51	19.204320580562374	28.774057852844354	28.515002445909765	23.50661912068351
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.96426614674466
2	38.839629308210576
3	38.70695594513653
4	43.20251224665108
5	42.293478977704424
6	43.27506653725715
7	38.17865510661123
8	38.10052379469012
9	42.434520620508366
10	38.523688238995085
11	42.95846985195871
12	42.93481522352935
13	38.4856457437891
14	42.8115588113862
15	38.37621525521022
16	42.73877126133349
17	42.72382145134452
18	38.461280734317704
19	42.69316056205658
20	38.41919860960405
21	42.70067207457764
22	38.34928423710007
23	38.39586177149618
24	42.73228978165239
25	38.40183138353176
26	42.710867009582195
27	42.692887170411886
28	38.46234717438709
29	42.682602167786705
30	38.531594425107954
31	42.71041762762906
32	38.48902975276564
33	42.68578226327018
34	42.648423529396794
35	38.39882875318881
36	42.60848282216793
37	38.38088062951717
38	42.653792535246644
39	42.62904923858423
40	38.38697740266096
41	38.37668536081783
42	42.58652537460446
43	38.41965308920039
44	42.61449409497164
45	42.55309718796042
46	38.33881280466379
47	38.26544365207709
48	38.30728797728493
49	42.62413728599963
50	42.67606062466682
51	42.710939701245884
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117.0
1	132.0
2	147.0
3	214.0
4	281.0
5	351.5
6	422.0
7	716.5
8	1011.0
9	1668.0
10	2325.0
11	3839.5
12	5354.0
13	8557.0
14	11760.0
15	17742.5
16	23725.0
17	34311.0
18	44897.0
19	62198.5
20	79500.0
21	105188.0
22	130876.0
23	165257.0
24	199638.0
25	240653.5
26	327943.5
27	374218.0
28	419392.5
29	464567.0
30	506100.0
31	547633.0
32	581421.0
33	615209.0
34	639030.5
35	662852.0
36	670886.0
37	678920.0
38	673934.0
39	668948.0
40	654000.5
41	639053.0
42	616351.0
43	593649.0
44	564670.0
45	535691.0
46	504910.5
47	474130.0
48	442671.0
49	411212.0
50	380525.0
51	349838.0
52	325983.0
53	302128.0
54	456812.5
55	611497.0
56	404199.0
57	196901.0
58	170433.0
59	143965.0
60	126678.5
61	109392.0
62	95156.5
63	80921.0
64	69983.0
65	59045.0
66	50745.0
67	42445.0
68	36353.0
69	30261.0
70	25542.5
71	20824.0
72	17408.5
73	13993.0
74	11496.0
75	7374.0
76	5749.0
77	4621.0
78	3493.0
79	2731.5
80	1970.0
81	1505.0
82	1040.0
83	804.5
84	569.0
85	432.5
86	296.0
87	228.5
88	161.0
89	138.0
90	115.0
91	89.0
92	63.0
93	48.0
94	33.0
95	30.5
96	28.0
97	23.0
98	18.0
99	14.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04774396764564243
2	3.1808106359521936E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0602702119840645E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	5.513405102317135E-4
20	0.0
21	0.0033186457635101217
22	1.3783512755792838E-4
23	0.002926345785076018
24	0.008651804929789966
25	0.0
26	0.04208212471364752
27	0.035126752123032054
28	0.05696831848990379
29	0.6329283030438874
30	0.06146386418871623
31	0.009627253524815306
32	0.08942318967873601
33	0.03431034405980433
34	0.016974926093864875
35	0.009796896758732757
36	0.01803519630584894
37	0.03718367633428114
38	0.03331369006053931
39	0.024884541875265998
40	0.033366703571138516
41	0.021724936643553485
42	0.02133263666511938
43	0.022901836578855795
44	0.013677485734594434
45	0.005333159166279845
46	0.03538121697390823
47	0.013868334372751565
48	0.014228826244826147
49	0.015204274839851487
50	0.017674704433774356
51	0.016211531541236345
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	9431558.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	22.681492915606626
#Duplication Level	Relative count
1	100.0
2	17.145850021566332
3	3.042085156201861
4	0.970484934376733
5	0.4929447285723088
6	0.3315053299648777
7	0.2384620124468544
8	0.14849959948240804
9	0.10844784028590794
10++	0.7579025201799249
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC	359815	3.8150112632504616	TruSeq Adapter, Index 7 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	461200	3.0390239	3.4538465	47
CACAC	892570	2.6461737	58.748768	12
GGAAG	811865	2.4899714	60.64439	5
GCACA	797075	2.3899388	59.10931	11
TCCAG	775215	2.3418343	58.724724	25
GTCTG	757755	2.3324952	60.27331	17
CTCCA	779265	2.3275964	58.11251	24
GAGCA	759565	2.3033712	59.564423	9
GAAGA	1083165	2.2664077	41.920383	6
ATGCC	747130	2.2569926	57.253735	47
GTCAC	744000	2.2475376	58.601475	29
AGAGC	733650	2.2247846	59.466896	8
CCAGT	735665	2.2223582	58.53915	26
GTGGA	717490	2.2170367	58.018517	36
AGTGG	708930	2.190586	58.391346	35
CAGTC	722860	2.1836758	58.434235	27
GAGTG	697810	2.1562254	58.588646	34
AGCAC	710170	2.1293638	58.96429	10
AAGAG	983000	2.0568233	41.56079	7
CTGAA	973080	2.0282753	41.280537	19
ACGTC	671315	2.0279644	59.05383	15
GATCG	662535	2.0242043	58.877407	1
CACGT	668555	2.0196269	59.0599	14
ACACG	666705	1.999039	58.670746	13
TCTCG	652595	1.9862056	57.94613	41
ACTCC	663380	1.9814581	57.936935	23
TCACG	655445	1.980023	58.11322	30
CGGAA	650255	1.9718902	59.87058	4
GGATC	642675	1.9635268	57.624836	38
CGTCT	644680	1.9621159	59.323288	16
ATCGG	639100	1.9526047	59.30699	2
CACGA	640540	1.9205863	57.45097	31
CTCGT	617920	1.8806704	57.475765	42
ACGAG	618920	1.8768673	58.008766	32
TCTGA	890605	1.8702943	41.37595	18
TGAAC	887270	1.8494142	41.031944	20
TCGGA	604975	1.8483446	60.121822	3
CGAGT	599170	1.8306091	57.668747	33
TGGAT	839140	1.7822587	40.128838	37
ATCTC	848800	1.7624576	39.71787	40
GTATG	814435	1.7297876	40.37557	45
AGTCA	822560	1.7145333	40.58623	28
TATGC	797365	1.6744878	39.75883	46
GATCT	780380	1.6388187	39.973484	39
GAACT	778020	1.6216949	40.819035	21
AACTC	783865	1.6155044	40.30456	22
TCGTA	652235	1.3697108	39.579494	43
CGTAT	650755	1.3666029	39.545067	44
>>END_MODULE