FastQCFastQC Report
Wed 23 Jul 2014
LW227_CGTACG_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW227_CGTACG_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5742321
Filtered Sequences 0
Sequence length 51
%GC 41

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC 362672 6.315773708923622 TruSeq Adapter, Index 12 (97% over 36bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CACCG 571790 3.8331487 129.20018 31
GGAAG 702515 3.6898572 105.35108 5
GAGCA 714135 3.5719445 99.94574 9
TTTTT 2033450 3.4943783 4.0157247 2
TCCAG 708620 3.4123774 94.706955 25
CGGAA 680350 3.4029593 100.892006 4
CTCCA 738020 3.3844044 90.26742 24
AAAAA 2030165 3.303115 3.5614066 14
TCGGA 630915 3.1903923 101.71277 3
GAAGA 892130 3.1705034 71.831894 6
AGAGC 626265 3.1324382 99.57582 8
TCTCG 630915 3.0715904 92.984955 41
AGCAC 643225 3.0637813 95.20084 10
ATCGG 596940 3.018588 100.43052 2
TCACC 656985 3.012795 88.61489 30
GTCTG 587030 3.0011127 100.929436 17
GCACA 627870 2.9906425 94.950325 11
CTGAA 868350 2.9710786 68.431526 19
CCAGT 610975 2.9421654 93.894745 26
CGTCT 592655 2.8853226 96.20351 16
CACAC 634435 2.8777456 90.2959 12
GATCG 568075 2.8726246 99.4756 1
CTCGT 582450 2.8356402 92.226425 42
GTCAC 587715 2.8301563 93.92944 29
CACGT 586835 2.8259187 95.30911 14
ATGCC 580560 2.7957013 91.04223 47
ACGTC 570270 2.746149 95.18783 15
CAGTC 568450 2.7373848 93.66609 27
ACACG 571745 2.7233107 94.38445 13
ACTCC 592255 2.7159564 89.98906 23
CGATC 559165 2.6926727 90.967995 38
ACCGT 556775 2.6811638 92.42767 32
TCTGA 766535 2.651552 68.914276 18
CCGTA 535870 2.5804956 92.3307 33
AAGAG 725140 2.5770447 71.15019 7
GTACG 508090 2.5692945 97.016266 35
TGAAC 723045 2.4739144 67.836586 20
CGTAC 509840 2.455147 91.93108 34
GAACT 680250 2.3274903 67.61884 21
AACTC 696780 2.2703114 64.42245 22
ACGAT 657510 2.2496848 65.46277 37
ATCTC 678595 2.235369 62.767204 40
GATCT 640465 2.2154582 65.62526 39
AGTCA 638220 2.1836836 66.7741 28
TCGTA 604835 2.092209 65.447716 43
GTATG 566285 2.0569928 68.49853 45
TACGA 599830 2.0523314 65.40083 36
TATGC 577730 1.9984488 65.14119 46
CGTAT 573485 1.9837648 65.29386 44
TGCCG 210770 1.5000519 5.243103 47
Produced by FastQC (version 0.10.1)