##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW227_CGTACG_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5742321	
Filtered Sequences	0	
Sequence length	51	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.669405280547707	34.0	31.0	34.0	30.0	34.0
2	31.99758547110132	34.0	31.0	34.0	30.0	34.0
3	32.380539506586274	34.0	31.0	34.0	30.0	34.0
4	36.03027765253806	37.0	35.0	37.0	35.0	37.0
5	36.002487670055366	37.0	35.0	37.0	35.0	37.0
6	36.00626541079818	37.0	35.0	37.0	35.0	37.0
7	35.97310303621131	37.0	35.0	37.0	35.0	37.0
8	36.00355953629203	37.0	35.0	37.0	35.0	37.0
9	37.4957916842336	39.0	37.0	39.0	35.0	39.0
10	37.63276800443584	39.0	37.0	39.0	35.0	39.0
11	37.65021234445096	39.0	37.0	39.0	35.0	39.0
12	37.63406208047234	39.0	37.0	39.0	35.0	39.0
13	37.659084715048145	39.0	37.0	39.0	35.0	39.0
14	39.08126870650387	40.0	38.0	41.0	36.0	41.0
15	39.04399527647444	40.0	38.0	41.0	36.0	41.0
16	39.05279572493422	40.0	38.0	41.0	36.0	41.0
17	39.00576491631171	40.0	38.0	41.0	36.0	41.0
18	38.88642884993716	40.0	38.0	41.0	35.0	41.0
19	38.98441292292785	40.0	38.0	41.0	36.0	41.0
20	38.9717025572064	40.0	38.0	41.0	36.0	41.0
21	38.907278607378444	40.0	38.0	41.0	35.0	41.0
22	38.829255452629695	40.0	38.0	41.0	35.0	41.0
23	38.77965895671802	40.0	38.0	41.0	35.0	41.0
24	38.72246622924772	40.0	38.0	41.0	35.0	41.0
25	38.671263762509966	40.0	38.0	41.0	34.0	41.0
26	38.59441783209263	40.0	38.0	41.0	34.0	41.0
27	38.49587684143746	40.0	38.0	41.0	34.0	41.0
28	38.24137643994475	40.0	38.0	41.0	34.0	41.0
29	38.0211917794216	40.0	38.0	41.0	33.0	41.0
30	37.87212191725262	40.0	38.0	41.0	33.0	41.0
31	38.20301999835955	40.0	38.0	41.0	34.0	41.0
32	38.175489144546255	40.0	38.0	41.0	34.0	41.0
33	38.2571773678274	40.0	38.0	41.0	34.0	41.0
34	38.277656717553754	40.0	38.0	41.0	34.0	41.0
35	38.373115156745854	40.0	38.0	41.0	34.0	41.0
36	38.33089564306837	40.0	38.0	41.0	34.0	41.0
37	38.33653482624883	40.0	38.0	41.0	34.0	41.0
38	38.230416063469804	40.0	38.0	41.0	33.0	41.0
39	38.16833837746096	40.0	38.0	41.0	33.0	41.0
40	37.96306998511577	40.0	38.0	41.0	33.0	41.0
41	38.06046370448465	40.0	38.0	41.0	33.0	41.0
42	38.091999907354534	40.0	38.0	41.0	33.0	41.0
43	38.076487538749575	40.0	38.0	41.0	33.0	41.0
44	38.04403236948962	40.0	38.0	41.0	33.0	41.0
45	37.84790209394424	40.0	37.0	41.0	33.0	41.0
46	37.610489730546234	40.0	37.0	41.0	32.0	41.0
47	37.56284575522685	40.0	37.0	41.0	32.0	41.0
48	37.60607844807004	40.0	37.0	41.0	32.0	41.0
49	37.53300886523063	40.0	37.0	41.0	32.0	41.0
50	37.47279175093137	40.0	37.0	41.0	32.0	41.0
51	36.44514700588839	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	13.0
15	13.0
16	58.0
17	134.0
18	264.0
19	586.0
20	1147.0
21	1948.0
22	3531.0
23	5810.0
24	9289.0
25	14200.0
26	20487.0
27	28676.0
28	39600.0
29	52729.0
30	68613.0
31	88083.0
32	112691.0
33	143845.0
34	190138.0
35	261914.0
36	384916.0
37	625911.0
38	1262533.0
39	2424573.0
40	616.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.485571792582267	25.728109414813687	29.003898338306072	20.782420454297974
2	18.761873465450645	34.376796420820085	28.272418069278952	18.58891204445032
3	18.009634083500384	27.49901999557322	35.87178424891259	18.619561672013806
4	17.941961099005088	26.912654308249223	29.024187258079092	26.12119733466659
5	25.564418986678035	27.750225039666017	28.951986487693738	17.733369485962207
6	25.61627954968035	28.154730465259604	27.62428293367786	18.604707051382185
7	18.616427051012995	35.395739806256046	28.22891649561214	17.758916647118824
8	18.024262314837504	35.626291180865714	28.124655518212933	18.22479098608385
9	25.26119683047801	27.889369395663806	28.355191815429208	18.494241958428976
10	18.122323708479552	35.257520434681375	27.943718228221652	18.676437628617418
11	25.381496436719576	27.831342065342568	28.036886130190215	18.75027536774764
12	18.02959117053888	27.885083400945366	27.97224327932904	26.113082149186713
13	18.022468615042595	35.34891205141614	27.932172374202004	18.696446959339262
14	18.00968632718373	28.20760107280662	27.965974037327417	25.816738562682236
15	17.93511717648665	35.45092655043144	28.0196979583691	18.594258314712814
16	18.029747901588923	28.131464611609136	28.00299739425922	25.83579009254272
17	25.398649779418463	28.003711391264964	28.065254450247558	18.532384379069022
18	18.179983320333363	27.86488251005125	35.26734224715059	18.687791922464804
19	18.20095059851693	27.816648343505545	27.98525741617829	25.997143641799237
20	18.317819571563483	27.79433612297188	35.084175893336514	18.803668412128125
21	25.536916737284965	27.831358285015096	27.831776247801777	18.799948729898166
22	18.271797745647486	35.104698210512346	27.860231955270994	18.76327208856917
23	18.257505872813553	35.117692245935615	27.84635754946286	18.778444331787973
24	18.307785657981952	27.796476862538565	27.89259631189725	26.003141167582232
25	18.250198830751536	27.813143848976747	35.04995628074432	18.886701039527395
26	18.315078747997177	27.845726643065117	27.807852203973543	26.031342404964164
27	18.377450852299948	27.86866654472288	27.726480092677274	26.027402510299897
28	18.441388543985905	34.9899828092531	27.68105780419136	18.88757084256963
29	25.701333346417037	27.828060483167228	27.72486859637306	18.745737574042675
30	18.56260390625667	27.711791030654233	34.777233523850285	18.948371539238817
31	18.441493752808363	27.762401206594472	27.6549954892037	26.14110955139347
32	18.459429729959144	34.91729670854861	27.618031215636158	19.005242345856086
33	18.498098147272298	27.76981963973239	27.621225146787143	26.110857066208165
34	18.585327161534572	27.793159280062092	27.659933563057272	25.961579995346067
35	25.64074296227715	27.87954659293333	27.716495883152447	18.76321456163707
36	18.503273775103704	27.910918343033664	34.793579387827045	18.792228494035584
37	18.446269255004257	35.00419231808449	27.68281790671191	18.866720520199344
38	18.597660450549057	27.86971603727685	27.626479682021117	25.906143830152985
39	25.693646572248145	27.90195165829293	27.576501089364086	18.827900680094842
40	18.585850737979772	34.81478603925916	27.70691732678985	18.892445895971218
41	18.535157413481198	27.81818606188825	34.6692684809694	18.977388043661154
42	18.569106727651207	27.675890117044965	27.800552051888072	25.95445110341576
43	18.517135380233487	27.68556361285216	34.67427170801992	19.12302929889443
44	18.664435454262353	27.694558215067232	27.773108131361933	25.86789819930848
45	25.51926085787411	27.7730194733271	27.85140668560545	18.856312983193334
46	18.59679697218704	27.90976766176322	34.67431865585229	18.81911671019746
47	18.62116039291214	34.76746126212175	27.866209367594536	18.745168977371566
48	18.780048642137523	27.75606267604211	34.62005906445745	18.843829617362918
49	25.65243342859935	27.692765629411436	27.61618241470342	19.0386185272858
50	18.760224115396298	27.681992737568727	27.52342315227418	26.03435999476079
51	18.836688079495346	27.621242696490654	27.537761577366425	26.00430764664757
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.26799224688024
2	37.350785509900966
3	36.62919575551419
4	44.06315843367168
5	43.29778847264024
6	44.220986601062535
7	36.375343698131815
8	36.249053300921354
9	43.755438788906986
10	36.79876133709697
11	44.13177180446722
12	44.142673319725596
13	36.71891557438185
14	43.82642488986596
15	36.52937549119946
16	43.86553799413164
17	43.93103415848748
18	36.86777524279816
19	44.198094240316166
20	37.121487983691615
21	44.33686546718313
22	37.035069834216664
23	37.03595020460153
24	44.31092682556418
25	37.13689987027893
26	44.34642115296134
27	44.40485336259984
28	37.32895938655553
29	44.447070920459716
30	37.51097544549549
31	44.58260330420183
32	37.46467207581523
33	44.60895521348046
34	44.546907156880636
35	44.40395752391422
36	37.295502269139284
37	37.312989775203604
38	44.503804280702035
39	44.521547252342984
40	37.47829663395099
41	37.51254545714235
42	44.52355783106697
43	37.64016467912792
44	44.53233365357083
45	44.37557384106744
46	37.4159136823845
47	37.366329370283715
48	37.62387825950044
49	44.69105195588514
50	44.794584110157096
51	44.840995726142914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19.0
1	21.5
2	24.0
3	51.5
4	79.0
5	173.5
6	268.0
7	557.0
8	846.0
9	1544.0
10	2242.0
11	3896.0
12	5550.0
13	8613.5
14	11677.0
15	17114.0
16	22551.0
17	31442.5
18	40334.0
19	52691.5
20	65049.0
21	81110.0
22	97171.0
23	116441.5
24	135712.0
25	155688.0
26	195716.0
27	215768.0
28	235500.0
29	255232.0
30	273252.5
31	291273.0
32	305024.0
33	318775.0
34	331015.0
35	343255.0
36	353984.5
37	364714.0
38	370976.5
39	377239.0
40	377629.5
41	378020.0
42	375767.0
43	373514.0
44	362751.5
45	351989.0
46	331001.5
47	310014.0
48	289393.5
49	268773.0
50	248385.0
51	227997.0
52	214044.0
53	200091.0
54	361607.5
55	523124.0
56	320699.0
57	118274.0
58	98894.0
59	79514.0
60	68910.0
61	58306.0
62	49707.5
63	41109.0
64	34916.5
65	28724.0
66	24467.5
67	20211.0
68	17002.5
69	13794.0
70	11629.5
71	9465.0
72	7961.5
73	6458.0
74	5292.5
75	3301.5
76	2476.0
77	1971.5
78	1467.0
79	1095.5
80	724.0
81	568.5
82	413.0
83	286.5
84	160.0
85	115.5
86	71.0
87	53.5
88	36.0
89	24.5
90	13.0
91	11.0
92	9.0
93	6.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04694965676770769
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.965824446247433E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	7.662406890872176E-4
20	0.0
21	0.0031868646841582
22	1.2190192780933007E-4
23	0.0032042792452738186
24	0.00882918248561862
25	0.0
26	0.04008831968815397
27	0.034324099958884226
28	0.055500206275476416
29	0.6178163846988004
30	0.058756729204097094
31	0.009421277563549652
32	0.08714246382255537
33	0.03249557104174427
34	0.01631744376533461
35	0.009560594052474601
36	0.01631744376533461
37	0.03587399589817428
38	0.03159001386373211
39	0.023631559433894413
40	0.03225176718612561
41	0.02088005877762668
42	0.020792985972048584
43	0.021193520877707813
44	0.013043506275598317
45	0.005450757629188616
46	0.03378424856430005
47	0.013635601353529349
48	0.013879405209148009
49	0.014872035192738267
50	0.01729265918780925
51	0.015725348687403575
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5742321.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	21.898467732298954
#Duplication Level	Relative count
1	100.0
2	11.227624192352504
3	1.736515670420977
4	0.8008803210582683
5	0.60904565194365
6	0.5313702644493874
7	0.46134472572349916
8	0.38543468794501523
9	0.3307088467558757
10++	1.6070566912638726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC	362672	6.315773708923622	TruSeq Adapter, Index 12 (97% over 36bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CACCG	571790	3.8331487	129.20018	31
GGAAG	702515	3.6898572	105.35108	5
GAGCA	714135	3.5719445	99.94574	9
TTTTT	2033450	3.4943783	4.0157247	2
TCCAG	708620	3.4123774	94.706955	25
CGGAA	680350	3.4029593	100.892006	4
CTCCA	738020	3.3844044	90.26742	24
AAAAA	2030165	3.303115	3.5614066	14
TCGGA	630915	3.1903923	101.71277	3
GAAGA	892130	3.1705034	71.831894	6
AGAGC	626265	3.1324382	99.57582	8
TCTCG	630915	3.0715904	92.984955	41
AGCAC	643225	3.0637813	95.20084	10
ATCGG	596940	3.018588	100.43052	2
TCACC	656985	3.012795	88.61489	30
GTCTG	587030	3.0011127	100.929436	17
GCACA	627870	2.9906425	94.950325	11
CTGAA	868350	2.9710786	68.431526	19
CCAGT	610975	2.9421654	93.894745	26
CGTCT	592655	2.8853226	96.20351	16
CACAC	634435	2.8777456	90.2959	12
GATCG	568075	2.8726246	99.4756	1
CTCGT	582450	2.8356402	92.226425	42
GTCAC	587715	2.8301563	93.92944	29
CACGT	586835	2.8259187	95.30911	14
ATGCC	580560	2.7957013	91.04223	47
ACGTC	570270	2.746149	95.18783	15
CAGTC	568450	2.7373848	93.66609	27
ACACG	571745	2.7233107	94.38445	13
ACTCC	592255	2.7159564	89.98906	23
CGATC	559165	2.6926727	90.967995	38
ACCGT	556775	2.6811638	92.42767	32
TCTGA	766535	2.651552	68.914276	18
CCGTA	535870	2.5804956	92.3307	33
AAGAG	725140	2.5770447	71.15019	7
GTACG	508090	2.5692945	97.016266	35
TGAAC	723045	2.4739144	67.836586	20
CGTAC	509840	2.455147	91.93108	34
GAACT	680250	2.3274903	67.61884	21
AACTC	696780	2.2703114	64.42245	22
ACGAT	657510	2.2496848	65.46277	37
ATCTC	678595	2.235369	62.767204	40
GATCT	640465	2.2154582	65.62526	39
AGTCA	638220	2.1836836	66.7741	28
TCGTA	604835	2.092209	65.447716	43
GTATG	566285	2.0569928	68.49853	45
TACGA	599830	2.0523314	65.40083	36
TATGC	577730	1.9984488	65.14119	46
CGTAT	573485	1.9837648	65.29386	44
TGCCG	210770	1.5000519	5.243103	47
>>END_MODULE