FastQCFastQC Report
Wed 23 Jul 2014
LW226_GTTTCG_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW226_GTTTCG_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8424700
Filtered Sequences 0
Sequence length 51
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC 212364 2.5207307085118758 TruSeq Adapter, Index 9 (97% over 36bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2865375 3.2407036 3.607802 3
TTTTT 2838300 3.1593072 3.5486457 4
CACGT 768160 2.5525568 38.730377 14
GAGCA 734050 2.541878 41.273544 9
CTCCA 784765 2.5104 37.81573 24
GAAGA 1013860 2.479878 29.900915 6
GGAAG 688735 2.4774315 43.03344 5
TCCAG 704485 2.3409681 38.890293 25
CGGAA 652730 2.260282 41.619072 4
CTGAA 933160 2.1902976 28.184824 19
TCGGA 596620 2.059406 41.218327 3
AGCAC 607685 2.025756 39.512295 10
TTTCG 865005 2.017418 26.873314 35
TCTCG 606035 2.0074124 37.993416 41
AGAGC 579575 2.00696 40.762985 8
GCACA 573960 1.9133314 39.27671 11
CACAC 593480 1.9045582 37.790356 12
GTTTC 816270 1.9037552 26.794865 34
TTCGA 798900 1.8691946 26.609123 36
TCTGA 782590 1.8310342 27.814215 18
ATCGG 526740 1.8181952 40.41521 2
AAGAG 730455 1.7866757 29.156086 7
CCAGT 537085 1.7847062 38.21435 26
CTCGT 529840 1.7550263 37.558247 42
CGTCT 524700 1.7380006 38.57608 16
GTCTG 496030 1.7067397 39.89711 17
GTCAC 510930 1.6977946 38.136765 29
ACTCC 523080 1.6732907 37.02111 23
TGAAC 711135 1.6691642 27.660748 20
GATCG 479600 1.6554778 39.8594 1
ACACG 492675 1.6423628 38.846252 13
ATGCC 492240 1.6356887 37.473988 47
ACGTC 491380 1.632831 38.65092 15
CGTTT 696310 1.6239771 26.807283 33
TCACG 485895 1.6146045 37.926205 30
CAGTC 481670 1.6005651 37.981194 27
TCGAT 682320 1.5964313 26.384981 37
CGATC 474080 1.5753438 36.682915 38
AACTC 680905 1.5385551 26.588703 22
GAACT 648905 1.523099 27.516346 21
ATCTC 667055 1.5024616 25.697777 40
GATCT 610695 1.4288495 26.536924 39
AGTCA 582305 1.3667766 27.020704 28
ACGTT 541695 1.2674094 26.626202 32
TCGTA 533915 1.2492065 26.44637 43
TATGC 487460 1.1405152 26.245848 46
GTATG 466945 1.1348757 27.276318 45
CGTAT 479270 1.121353 26.273157 44
Produced by FastQC (version 0.10.1)