##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	LW226_GTTTCG_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	8424700	
Filtered Sequences	0	
Sequence length	51	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.751618455256565	34.0	31.0	34.0	30.0	34.0
2	32.0625581919831	34.0	31.0	34.0	30.0	34.0
3	32.44765783944829	34.0	31.0	34.0	30.0	34.0
4	36.07766994670433	37.0	35.0	37.0	35.0	37.0
5	36.04291155768158	37.0	35.0	37.0	35.0	37.0
6	36.056139209704796	37.0	35.0	37.0	35.0	37.0
7	36.03997151233872	37.0	35.0	37.0	35.0	37.0
8	36.044620579961304	37.0	35.0	37.0	35.0	37.0
9	37.555417284888485	39.0	38.0	39.0	35.0	39.0
10	37.6865322207319	39.0	37.0	39.0	35.0	39.0
11	37.71687834581647	39.0	38.0	39.0	35.0	39.0
12	37.69394898334659	39.0	37.0	39.0	35.0	39.0
13	37.69945754744976	39.0	37.0	39.0	35.0	39.0
14	39.12164278846725	40.0	39.0	41.0	36.0	41.0
15	39.086458034113974	40.0	38.0	41.0	36.0	41.0
16	39.07999109760585	40.0	38.0	41.0	36.0	41.0
17	39.039466449843914	40.0	38.0	41.0	36.0	41.0
18	39.00688356855437	40.0	38.0	41.0	36.0	41.0
19	39.04674124894655	40.0	39.0	41.0	36.0	41.0
20	39.01348119220862	40.0	39.0	41.0	36.0	41.0
21	38.959471553883226	40.0	38.0	41.0	35.0	41.0
22	38.889997982123994	40.0	38.0	41.0	35.0	41.0
23	38.80250062316759	40.0	38.0	41.0	35.0	41.0
24	38.73532351300343	40.0	38.0	41.0	35.0	41.0
25	38.705067361449075	40.0	38.0	41.0	35.0	41.0
26	38.60850985791779	40.0	38.0	41.0	34.0	41.0
27	38.50705805547972	40.0	38.0	41.0	34.0	41.0
28	38.356562607570595	40.0	38.0	41.0	34.0	41.0
29	38.07693152278419	40.0	38.0	41.0	34.0	41.0
30	38.012467031467	40.0	38.0	41.0	33.0	41.0
31	38.29546607000843	40.0	38.0	41.0	34.0	41.0
32	38.28465666433226	40.0	38.0	41.0	34.0	41.0
33	38.31477797428989	40.0	38.0	41.0	34.0	41.0
34	38.44704001329424	40.0	38.0	41.0	34.0	41.0
35	38.44481524564673	40.0	38.0	41.0	34.0	41.0
36	38.48501738934324	40.0	38.0	41.0	34.0	41.0
37	38.46753866606526	40.0	38.0	41.0	34.0	41.0
38	38.33299690196684	40.0	38.0	41.0	34.0	41.0
39	38.278577990907685	40.0	38.0	41.0	34.0	41.0
40	38.14831804099849	40.0	38.0	41.0	33.0	41.0
41	38.21228198036725	40.0	38.0	41.0	33.0	41.0
42	38.185393663869334	40.0	38.0	41.0	33.0	41.0
43	38.155445416454	40.0	38.0	41.0	33.0	41.0
44	38.09005163388607	40.0	38.0	41.0	33.0	41.0
45	37.94190546844398	40.0	38.0	41.0	33.0	41.0
46	37.85554880292473	40.0	37.0	41.0	33.0	41.0
47	37.7718915807091	40.0	37.0	41.0	33.0	41.0
48	37.749220150272414	40.0	37.0	41.0	33.0	41.0
49	37.67336463019455	40.0	37.0	41.0	32.0	41.0
50	37.594053082008855	40.0	37.0	41.0	32.0	41.0
51	36.55194202760929	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	4.0
12	3.0
13	6.0
14	12.0
15	32.0
16	84.0
17	191.0
18	398.0
19	903.0
20	1755.0
21	2766.0
22	4908.0
23	8128.0
24	12849.0
25	19450.0
26	28055.0
27	39326.0
28	53824.0
29	72299.0
30	94441.0
31	121017.0
32	154620.0
33	199705.0
34	265854.0
35	368143.0
36	543823.0
37	896747.0
38	1860217.0
39	3674187.0
40	948.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.717605166209857	26.205722311113387	30.65301222304047	23.423660299636275
2	19.788410244686485	31.142631920736875	28.51183169867553	20.557126135901115
3	19.317886690327253	28.93165335264164	31.774199674765867	19.976260282265244
4	19.109986112265126	27.801322302277825	30.06391919000083	23.024772395456218
5	22.2613980319774	28.87789476183128	30.407041200280126	18.45366600591119
6	22.178130972022743	29.376606882144173	28.530084157299367	19.915177988533717
7	19.977696535188198	31.88178807553978	29.475601505098105	18.664913884173917
8	19.100668273054232	32.29227153489145	29.253279048512116	19.353781143542204
9	21.81994460096839	28.80695042289953	29.616107216212907	19.75699775991917
10	19.116645103089724	31.78782627274562	29.03260650230869	20.062922121855973
11	21.993922632259903	28.79849727586739	29.07713034292022	20.130449748952483
12	19.192374802663597	28.87999572685081	28.951642194974305	22.975987275511294
13	19.1134521110544	32.00828516148943	28.914810022908828	19.963452704547343
14	19.130770235141902	29.27376642491721	28.959357603238097	22.636105736702792
15	19.029745866321647	32.11782021911759	29.034019015513906	19.81841489904685
16	19.06823981862856	29.151601837454155	29.104300449867647	22.67585789404964
17	22.026113689508232	29.12278182012416	29.10877538666065	19.742329103706957
18	19.231485987631608	28.91396726292924	31.956734364428407	19.897812385010745
19	19.22771683496632	28.87630070592793	29.02711992038607	22.868862538719682
20	19.327572495163032	28.82597599914537	31.836825050150154	20.009626455541444
21	22.25845171788269	28.789945660108955	28.888397985751357	20.063204636256998
22	19.385833635619505	31.715845144651055	28.87805459383184	20.0202666258976
23	19.390059856612872	31.76209625630871	28.9049537383141	19.942890148764324
24	19.41168447277256	28.82833453011479	28.890846527111936	22.86913447000071
25	19.31041097135933	28.895240055460736	31.738938091438047	20.05541088174189
26	19.396901883149738	28.86669371792191	28.887486846950576	22.848917551977777
27	19.440914534649966	28.919033038768266	28.77782609222243	22.862226334359338
28	19.44328678616709	31.74100258023522	28.75148088874109	20.0642297448566
29	22.358659873466046	28.911063574209088	28.796771143263555	19.933505409061304
30	19.605831958665053	28.76276766837217	31.52807488520545	20.103325487757324
31	19.489254651395242	28.77315844729843	28.75080544411369	22.986781457192635
32	19.55704090397682	31.641861796857086	28.699838304009845	20.10125899515625
33	19.578914821170482	28.801471995653166	28.717273483322497	22.902339699853854
34	22.366958459448085	28.866198951350526	28.74504568623738	20.021796902964013
35	19.591321141804073	28.904356654048097	31.58306788589082	19.921254318257013
36	19.523109582150433	28.836085213241457	31.633126368030208	20.007678836577902
37	19.51494215230497	28.874699564009354	31.586374464154947	20.02398381953072
38	19.630361149021795	28.920466567765104	28.74197829880922	22.707193984403887
39	22.479179942076765	28.89535409028668	28.651899839561214	19.97356612807534
40	19.601900115010963	31.66161747254686	28.731972690893144	20.004509721549034
41	19.622268575479502	28.89543174521122	31.453952666567492	20.02834701274179
42	19.66059259642698	28.79813810090186	28.677501229401443	22.863768073269714
43	19.58329093803646	28.76660495652765	31.5322940559162	20.117810049519687
44	19.650412976486447	28.807053900038014	28.71076355359933	22.831769569876208
45	22.40289424496146	28.846570687234795	28.744993866455147	20.005541201348596
46	19.544819441372013	28.929051949203892	31.500763091021504	20.025365518402587
47	19.666228602837563	31.693253223449354	28.744739375764976	19.895778797948104
48	19.733499442089695	28.8398255478164	31.490644451401256	19.936030558692654
49	22.493450914924935	28.84056085288997	28.680160801318817	19.985827430866284
50	19.744825630174535	28.765586743982592	28.67806555375139	22.811522072091485
51	19.777197399433305	28.72673356965567	28.604583437657787	22.89148559325324
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.14126546584613
2	40.3455363805876
3	39.2941469725925
4	42.134758507721344
5	40.71506403788859
6	42.093308960556456
7	38.642610419362114
8	38.45444941659644
9	41.576942360887564
10	39.17956722494569
11	42.12437238121239
12	42.168362078174894
13	39.07690481560174
14	41.7668759718447
15	38.8481607653685
16	41.7440977126782
17	41.768442793215186
18	39.12929837264235
19	42.096579373686
20	39.337198950704476
21	42.321656354139684
22	39.4061002615171
23	39.33295000537719
24	42.280818942773266
25	39.36582185310122
26	42.245819435127515
27	42.303140869009304
28	39.50751653102369
29	42.29216528252736
30	39.70915744642238
31	42.47603610858787
32	39.65829989913306
33	42.48125452102434
34	42.3887553624121
35	39.512575460061086
36	39.530788418728335
37	39.538925971835695
38	42.33755513342568
39	42.4527460701521
40	39.60640983656
41	39.65061558822129
42	42.5243606696967
43	39.701100987556146
44	42.48218254636266
45	42.40843544631006
46	39.5701849597746
47	39.56200740078567
48	39.669530000782345
49	42.479278345791215
50	42.55634770226602
51	42.66868299268655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	17.0
2	15.0
3	46.0
4	77.0
5	166.0
6	255.0
7	532.5
8	810.0
9	1552.0
10	2294.0
11	4042.0
12	5790.0
13	9547.5
14	13305.0
15	19901.0
16	26497.0
17	37400.5
18	48304.0
19	64559.0
20	80814.0
21	102587.5
22	124361.0
23	150091.5
24	175822.0
25	204722.5
26	263439.5
27	293256.0
28	324059.5
29	354863.0
30	384523.0
31	414183.0
32	440364.0
33	466545.0
34	491956.0
35	517367.0
36	540921.0
37	564475.0
38	582069.5
39	599664.0
40	609806.0
41	619948.0
42	621912.0
43	623876.0
44	612404.5
45	600933.0
46	568052.5
47	535172.0
48	500480.0
49	465788.0
50	432647.0
51	399506.0
52	461529.0
53	523552.0
54	377807.5
55	232063.0
56	197244.0
57	162425.0
58	139859.0
59	117293.0
60	99830.5
61	82368.0
62	67933.5
63	53499.0
64	43525.0
65	33551.0
66	27636.5
67	21722.0
68	17309.5
69	12897.0
70	10170.5
71	7444.0
72	5920.5
73	4397.0
74	3491.5
75	2022.5
76	1459.0
77	1177.0
78	895.0
79	717.5
80	540.0
81	377.5
82	215.0
83	165.0
84	115.0
85	87.5
86	60.0
87	42.5
88	25.0
89	20.0
90	15.0
91	12.0
92	9.0
93	7.0
94	5.0
95	2.5
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05093356439991928
2	1.1869858867378067E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.747943546951227E-5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	5.22273790164635E-4
20	0.0
21	0.003774615119826225
22	1.424383064085368E-4
23	0.002777546974966468
24	0.008510688807910075
25	1.1869858867378067E-5
26	0.043313115007062565
27	0.035823234061747006
28	0.05845905492183697
29	0.6313815328735741
30	0.0604531912115565
31	0.00921101048108538
32	0.09104181751278977
33	0.03484990563462201
34	0.017626740418056428
35	0.010362386791221052
36	0.018386411385568624
37	0.03785297992806866
38	0.033924056642966514
39	0.025793203318812536
40	0.034351371562192125
41	0.023063135779315583
42	0.022576471565753084
43	0.023609149287214977
44	0.014457488100466485
45	0.006338504635179888
46	0.03597754222702292
47	0.015074720761570144
48	0.015466426104193621
49	0.016451624390186
50	0.018956164611202773
51	0.017318124087504598
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	8424700.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	15.971097904886356
#Duplication Level	Relative count
1	100.0
2	7.724104809984735
3	1.1880599404254832
4	0.5494348425163087
5	0.3876345715151481
6	0.3390373169741634
7	0.29672911890318854
8	0.33389172531688266
9	0.3047333725922919
10++	3.2228555746768284
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC	212364	2.5207307085118758	TruSeq Adapter, Index 9 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2865375	3.2407036	3.607802	3
TTTTT	2838300	3.1593072	3.5486457	4
CACGT	768160	2.5525568	38.730377	14
GAGCA	734050	2.541878	41.273544	9
CTCCA	784765	2.5104	37.81573	24
GAAGA	1013860	2.479878	29.900915	6
GGAAG	688735	2.4774315	43.03344	5
TCCAG	704485	2.3409681	38.890293	25
CGGAA	652730	2.260282	41.619072	4
CTGAA	933160	2.1902976	28.184824	19
TCGGA	596620	2.059406	41.218327	3
AGCAC	607685	2.025756	39.512295	10
TTTCG	865005	2.017418	26.873314	35
TCTCG	606035	2.0074124	37.993416	41
AGAGC	579575	2.00696	40.762985	8
GCACA	573960	1.9133314	39.27671	11
CACAC	593480	1.9045582	37.790356	12
GTTTC	816270	1.9037552	26.794865	34
TTCGA	798900	1.8691946	26.609123	36
TCTGA	782590	1.8310342	27.814215	18
ATCGG	526740	1.8181952	40.41521	2
AAGAG	730455	1.7866757	29.156086	7
CCAGT	537085	1.7847062	38.21435	26
CTCGT	529840	1.7550263	37.558247	42
CGTCT	524700	1.7380006	38.57608	16
GTCTG	496030	1.7067397	39.89711	17
GTCAC	510930	1.6977946	38.136765	29
ACTCC	523080	1.6732907	37.02111	23
TGAAC	711135	1.6691642	27.660748	20
GATCG	479600	1.6554778	39.8594	1
ACACG	492675	1.6423628	38.846252	13
ATGCC	492240	1.6356887	37.473988	47
ACGTC	491380	1.632831	38.65092	15
CGTTT	696310	1.6239771	26.807283	33
TCACG	485895	1.6146045	37.926205	30
CAGTC	481670	1.6005651	37.981194	27
TCGAT	682320	1.5964313	26.384981	37
CGATC	474080	1.5753438	36.682915	38
AACTC	680905	1.5385551	26.588703	22
GAACT	648905	1.523099	27.516346	21
ATCTC	667055	1.5024616	25.697777	40
GATCT	610695	1.4288495	26.536924	39
AGTCA	582305	1.3667766	27.020704	28
ACGTT	541695	1.2674094	26.626202	32
TCGTA	533915	1.2492065	26.44637	43
TATGC	487460	1.1405152	26.245848	46
GTATG	466945	1.1348757	27.276318	45
CGTAT	479270	1.121353	26.273157	44
>>END_MODULE