FastQCFastQC Report
Wed 23 Jul 2014
LW225_GTGGCC_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LW225_GTGGCC_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9287907
Filtered Sequences 0
Sequence length 51
%GC 39

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC 218624 2.3538564716464108 TruSeq Adapter, Index 1 (97% over 35bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 3814250 3.4279227 3.6232622 3
TTTTT 3851445 3.3731549 3.6561723 5
GTGGC 527780 2.7729576 60.745415 34
CACGT 785955 2.5407634 38.86751 14
CGTGG 481935 2.5320878 60.93336 33
GAGCA 740130 2.489049 41.17301 9
GGAAG 705650 2.4560187 42.791542 5
CTCCA 783795 2.4482293 37.882607 24
CGGAA 709180 2.384964 41.66402 4
GGCCA 467040 2.3832483 58.400063 36
TGGCC 465595 2.3636417 57.93553 35
TCCAG 715315 2.312405 38.99145 25
GAAGA 1042375 2.310253 27.893745 6
CTGAA 1039195 2.2139804 26.612173 19
TCGGA 614325 2.0553308 41.032406 3
TCTCG 624450 2.008271 37.90778 41
AGCAC 615790 2.0009725 39.614143 10
AGAGC 589540 1.9826164 40.734016 8
CCATC 626480 1.9568468 36.253468 38
GCACA 597065 1.9401264 39.415024 11
CACAC 606750 1.9050275 38.070934 12
ATCGG 547915 1.8331447 40.187225 2
TCTGA 853830 1.8096985 26.119131 18
CCAGT 554265 1.7917771 38.34144 26
ACGTG 527690 1.765478 39.075653 32
GTCAC 536805 1.735334 38.236134 29
CTCGT 538365 1.7314163 37.388317 42
GTCTG 513355 1.7086749 39.875423 17
CGTCT 528665 1.7002205 38.603317 16
AAGAG 754080 1.6712945 27.239668 7
ACTCC 530760 1.6578597 37.290203 23
GCCAT 512710 1.6574419 37.14452 37
ACACG 508755 1.6531684 39.07172 13
TGAAC 775495 1.6521738 26.034481 20
ATGCC 503505 1.6276848 37.370773 47
GATCG 483195 1.6166126 39.577644 1
TCACG 500055 1.6165322 37.9838 30
ACGTC 495760 1.6026475 38.731762 15
CAGTC 489670 1.5829602 38.161175 27
AACTC 724145 1.490683 24.99923 22
GAACT 690750 1.4716265 25.78085 21
CATCT 715935 1.466194 24.097904 39
ATCTC 696305 1.4259932 24.29403 40
AGTCA 628115 1.3381841 25.360622 28
TCGTA 564475 1.1964086 24.573133 43
TATGC 512275 1.0857704 24.417978 46
GTATG 491920 1.0790615 25.25489 45
CGTAT 508855 1.0785217 24.420204 44
Produced by FastQC (version 0.10.1)